1.
J Biol Chem
; 287(46): 38427-32, 2012 Nov 09.
Article
in English
| MEDLINE
| ID: mdl-23019344
ABSTRACT
Within the past 2 years, transcription activator-like effector (TALE) DNA binding domains have emerged as the new generation of engineerable platform for production of custom DNA binding domains. However, their recently described sensitivity to cytosine methylation represents a major bottleneck for genome engineering applications. Using a combination of biochemical, structural, and cellular approaches, we were able to identify the molecular basis of such sensitivity and propose a simple, drug-free, and universal method to overcome it.