Effects of chironomid larvae density and mosquito biocide on methane and carbon dioxide dynamics in freshwater sediments.

Small lentic water bodies are important emitters of methane (CH4) and carbon dioxide (CO2), but the processes regulating their dynamics and susceptibility to human-induced stressors are not fully understood. Bioturbation by chironomid larvae has been proposed as a potentially important factor controlling the dynamics of both gases in aquatic sediments. Chironomid abundance can be affected by the application of biocides for mosquito control, such as Bti (Bacillus thuringiensis var. israelensis). Previous research has attributed increases in CH4 and CO2 emissions after Bti application to reduced bioturbation by chironomids. In this study, we separately tested the effect of chironomid bioturbation and Bti addition on CH4 production and emission from natural sediments. In a set of 15 microcosms, we compared CH4 and CO2 emission and production rates with high and low densities of chironomid larvae at the bioturbating stage, and standard and five times (5x) standard Bti dose, with control sediments that contained neither chironomid larvae nor Bti. Regardless of larvae density, chironomid larvae did not affect CH4 nor CO2 emission and production of the sediment, although both rates were more variable in the treatments with organisms. 5xBti dosage, however, led to a more than three-fold increase in CH4 and CO2 production rates, likely stimulated by bioavailable dissolved carbon in the Bti excipient and priming effects. Our results suggest weak effects of bioturbating chironomid larvae on the CH4 and CO2 dynamics in aquatic ecosystems. Furthermore, our results point out towards potential functional implications of Bti for carbon cycling beyond those mediated by changes in the macroinvertebrate community.

Organic Matter Accumulation and Hydrology as Drivers of Greenhouse Gas Dynamics in Newly Developed Artificial Channels.

Artificial channels, common features of inland waters, have been suggested as significant contributors to methane (CH4) and carbon dioxide (CO2) dynamics and emissions; however, the magnitude and drivers of their CH4 and CO2 emissions (diffusive and ebullitive) remain unclear. They are characterized by reduced flow compared to the donor river, which results in suspended organic matter (OM) accumulation. We propose that in such systems hydrological controls will be reduced and OM accumulation will control emissions by promoting methane production and outgassing. Here, we monitored summertime CH4 and CO2 concentrations and emissions on six newly constructed river-fed artificial channels, from bare riparian mineral soil to lotic channels, under two distinct flow regimes. Chamber-based fluxes were complemented with hydrology, total fluxes (diffusion + ebullition), and suspended OM accumulation assessments. During the first 6 weeks after the flooding, inflowing riverine water dominated the emissions over in-channel contributions. Afterwards, a substantial accumulation of riverine suspended OM (≥50% of the channel's volume) boosted in-channel methane production and led to widespread ebullition 10× higher than diffusive fluxes, regardless of the flow regime. Our finding suggests ebullition as a dominant pathway in these anthropogenic systems, and thus, their impact on regional methane emissions might have been largely underestimated.

Does biocide treatment for mosquito control alter carbon dynamics in floodplain ponds?

Shallow lentic aquatic ecosystems, such as ponds, are important repositories of carbon (C) and hotspots of C cycling and greenhouse gas emission. Tube-dwelling benthic invertebrates, such as chironomids, may be key players in C dynamics in these water bodies, yet their role in the C-budget at ecosystem level remains unclear. We tested whether a 41 % reduction in chironomid abundance after application of the mosquito control biocide Bacillus thuringiensis israelensis (Bti) had implications for the C-fluxes to the atmosphere, C-pools, and C-transformation (i.e. organic matter decomposition) in ponds. Data were collected over one year in the shallow, deep and riparian zones of 12 experimental floodplain pond mesocosms (FPMs), half of them treated with Bti. C-fluxes were measured as CO2 and CH4 emissions, atmospheric deposition, and emerging insects. C-pools were measured as dissolved inorganic and organic C in surface and porewater, sediment organic C, C in plant and in macroinvertebrate biomass. Despite seasonal variability, treated FPMs, for which higher CH4 emissions have been reported, showed a trend towards less dissolved organic C in porewater, while no effect was observed for all remaining components of the C-budget. We attribute the effect of Bti on the C-budget to the reduction in macroinvertebrates biomass, the increase in CH4 emissions, and the input of C from the Bti excipients. This finding suggests that changes in tube-dwelling macroinvertebrates have a weak influence on C cycling in ponds and confirms the existence of long-lasting effects of Bti on specific components of C-budgets.

Carbon limitation may override fine-sediment induced alterations of hyporheic nitrogen and phosphorus dynamics.

The hyporheic zone underneath stream channels is considered a biogeochemical hotspot reducing nutrient loads being transported downstream due to its high surface-to-volume ratio in combination with the hyporheic exchange. However, the effect of environmental stressors such as high amounts of fine sediment (FS; grain size <0.2 mm) on nutrient cycling in the hyporheic zone are not well understood. Physical clogging caused by fine sediment (FS) decreases the hyporheic exchange, thus, diminishing its potential to reduce nutrient loads despite increasing its surface-to-volume ratio. We determined the effect of physical clogging on nutrient cycling based on net change rates of dissolved inorganic nitrogen (DIN; nitrate-N, ammonium-N), soluble reactive phosphorus (SRP), and dissolved organic carbon (DOC) for a sand and gravel hyporheic zone. We performed three experimental runs in 12 flumes with four-week duration each following a factorial design. First, we determined nutrient cycling in sand and gravel in absence of clogging, and then tested the clogging effect for each sediment type under increasing clogging (0-480 g of FS addition increasing by 60 g per level). Without clogging, gravel acted as a source of nitrate-N; and both sand and gravel released SRP. Regardless of the clogging level and the resulting reduced hyporheic exchange, we found no changes in DOC and nitrate-N dynamics but net-release of ammonium-N and SRP for gravel. In contrast, in sand, physical clogging inhibited DOC release for flumes with the higher FS. We propose that not physical clogging but DOC availability limited the nutrient uptake, as molar ratios of DOC to DIN and SRP ranged 1.2-1.5 and 77-191, respectively, indicating severe C limitation of N-uptake and partial C limitation of P-uptake. Our results suggest an interplay between nutrient molar ratios and physical clogging, which emphasize the interactions between hydrology and the stoichiometry of organic carbon, nitrogen and phosphorus in the hyporheic zone.

When water returns: Drying history shapes respiration and nutrients release of intermittent river sediment.

Climate change and anthropogenic water demand have increased the frequency and duration of drying periods across rivers and streams worldwide. However, the biogeochemical processes during the water return in desiccated riverbeds are still unclear. Drying is a complex and diverse process and biogeochemical implications upon flow resumption may depend on attributes of the drying and river sediment characteristics (i.e., organic matter content [OM]). In order to understand the effect of drying duration and intensity on the biogeochemical dynamics following flow resumption, we exposed OM- and non-enriched river sediment from an intermittent river section to three different drying intensities (low: shade and rain; moderate: no shade and rain; high: no shade and no rain), each for three drying durations (10, 30 and 90 days). We determined the sediment-associated microbial respiration and dissolved organic carbon (DOC), ammonium­nitrogen (NH4-N), nitrate­nitrogen (NO3-N) and soluble reactive phosphorus (SRP) net release/retention rates of the nine drying treatments in flow-through microcosms over four days past flow resumption. Under the most intense and prolonged drying, non-enriched sediments showed a lag response in respiration on the first day after flow resumption, while all other treatments had either a linear increase or an early pulse in respiration. After 48 h, respiration remained constant, with minor changes in respiration dynamics regardless of the OM content of the sediment and drying attributes. The drying duration and intensity had greater effects on SRP release/retention soon after the flow resumption, while NH4-N and NO3-N release/retention rates were more strongly affected four days later. Our results suggest that drying attributes influence the biogeochemical dynamics more strongly during the first 24 h upon flow resumption. However, neither respiration nor nutrient dynamics recovered within four days to levels of the sediments before drying for any drying treatments. Hence, the atrributes of the drying have considerable implications in rivers biogeochemistry upon flow resumption.

Attributes of Drying Define the Structure and Functioning of Microbial Communities in Temperate Riverbed Sediment.

Combined effects of climate change and increasing anthropogenic water demand have increased and extended dry period occurrences in rivers worldwide. Riverbed drying can significantly affect sediment microorganisms, crucial drivers of biogeochemical processes in lotic systems. In this study, we evaluated how sediment bacterial and fungal community structure and composition (based on 16S rRNA gene and ITS metabarcoding) and microbial functions (community respiration and extracellular enzymatic activities) respond to different riverbed drying intensities over 90 days. Riverbed sediment collected in a flowing reach of the Spree river in northeastern Germany was dried under different rates in outdoor mesocosms during the summer months of 2018. Our results demonstrate that drying attributes (duration and intensity) and sediment organic matter (OM) content play a crucial role in sediment microbial community assembly and functioning throughout drying. Milder drying surprisingly triggered a more rapid and drastic change in the microbial community composition and diversity. After 90 days of drying, Bacilli (Firmicutes) became the dominant bacterial class in most treatments, except in sediments with low OM content under the most severe drying treatment. Fungal amplicon sequence variants (ASVs) from Dothideomycetes (Ascomycota) had by far the highest relative abundance in all our treatments at the end of the drying experiment, making up 65.1% to 94.0% of the fungal reads. CO2 fluxes, a proxy for sediment community respiration, were rapidly and strongly affected by drying in all treatments. Our results imply that even short riverbed drying periods are likely to have significant consequences for the biogeochemical dynamics in recently formed non-perennial temperate rivers.

The method controls the story - Sampling method impacts on the detection of pore-water nitrogen concentrations in streambeds.

Biogeochemical gradients in streambeds are steep and can vary over short distances often making adequate characterisation of sediment biogeochemical processes challenging. This paper provides an overview and comparison of streambed pore-water sampling methods, highlighting their capacity to address gaps in our understanding of streambed biogeochemical processes. This work reviews and critiques available pore-water sampling techniques to characterise streambed biogeochemical conditions, including their characteristic spatial and temporal resolutions, and associated advantages and limitations. A field study comparing three commonly-used pore-water sampling techniques (multilevel mini-piezometers, miniature drivepoint samplers and diffusive equilibrium in thin-film gels) was conducted to assess differences in observed nitrate and ammonium concentration profiles. Pore-water nitrate concentrations did not differ significantly between sampling methods (p-value = 0.54) with mean concentrations of 2.53, 4.08 and 4.02 mg l-1 observed with the multilevel mini-piezometers, miniature drivepoint samplers and diffusive equilibrium in thin-film gel samplers, respectively. Pore-water ammonium concentrations, however, were significantly higher in pore-water extracted by multilevel mini-piezometers (3.83 mg l-1) and significantly lower where sampled with miniature drivepoint samplers (1.05 mg l-1, p-values <0.01). Differences in observed pore-water ammonium concentration profiles between active (suction: multilevel mini-piezometers) and passive (equilibrium; diffusive equilibrium in thin-film gels) samplers were further explored under laboratory conditions. Measured pore-water ammonium concentrations were significantly greater when sampled by diffusive equilibrium in thin-film gels than with multilevel mini-piezometers (all p-values ≤0.02). The findings of this study have critical implications for the interpretation of field-based research on hyporheic zone biogeochemical cycling and highlight the need for more systematic testing of sampling protocols. For the first time, the impact of different active and passive pore-water sampling methods is addressed systematically here, highlighting to what degree the choice of pore-water sampling methods affects research outcomes, with relevance for the interpretation of previously published work as well as future studies.

The interplay between total mercury, methylmercury and dissolved organic matter in fluvial systems: A latitudinal study across Europe.

Large-scale studies are needed to identify the drivers of total mercury (THg) and monomethyl-mercury (MeHg) concentrations in aquatic ecosystems. Studies attempting to link dissolved organic matter (DOM) to levels of THg or MeHg are few and geographically constrained. Additionally, stream and river systems have been understudied as compared to lakes. Hence, the aim of this study was to examine the influence of DOM concentration and composition, morphological descriptors, land uses and water chemistry on THg and MeHg concentrations and the percentage of THg as MeHg (%MeHg) in 29 streams across Europe spanning from 41°N to 64 °N. THg concentrations (0.06-2.78 ng L-1) were highest in streams characterized by DOM with a high terrestrial soil signature and low nutrient content. MeHg concentrations (7.8-159 pg L-1) varied non-systematically across systems. Relationships between DOM bulk characteristics and THg and MeHg suggest that while soil derived DOM inputs control THg concentrations, autochthonous DOM (aquatically produced) and the availability of electron acceptors for Hg methylating microorganisms (e.g. sulfate) drive %MeHg and potentially MeHg concentration. Overall, these results highlight the large spatial variability in THg and MeHg concentrations at the European scale, and underscore the importance of DOM composition on mercury cycling in fluvial systems.

Contrasting habitats but comparable microbial decomposition in the benthic and hyporheic zone.

Input of allochthonous leaf litter is the main carbon source for heterotrophic metabolism in low-order forested streams. A major part of this leaf litter is accumulated at benthic retention structures or buried in the hyporheic zone. As a result of hyporheic sediment characteristics, hyporheic physicochemistry differs from that of the benthic zone selecting the microbial community. The present study aimed at understanding the influence of the hydrological and physiochemical differences between the benthic and hyporheic zone on microbial leaf litter decomposition and on the structure and function of the associated microbial community. Leached leaves of Alnus glutinosa were exposed for 62days in 250-µm mesh bags in the benthic zone and buried in the hyporheic zone at a depth of 2-3cm. Decomposition rates were comparable for both zones. In contrast, respiration, bacterial abundance, ergosterol content, fungal spore production and richness of fungal morphotypes were lower associated with hyporheic than with benthic leaves. Microbial community structure displayed zone-dependent temporal dynamics. Thus, the microbial community carried out leaf litter decomposition independently of its structure. These results suggest that carbon processing is not necessarily impaired by environmental constraints because the community structure may compensate those constraints (i.e. functional redundancy).

Relating hydraulic conductivity and hyporheic zone biogeochemical processing to conserve and restore river ecosystem services.

River management practices commonly attempt to improve habitat and ecological functioning (e.g. biogeochemical processing or retention of pollutants) by restoring hydrological exchange with the hyporheic zone (i.e. hyporheic flow) in an effort to increase mass transfer of solutes (nutrients, carbon and electron acceptors such as oxygen or nitrate). However, even when hyporheic flow is increased, often no significant changes in biogeochemical processing are detected. Some of these apparent paradox result from the simplistic assumption that there is a direct relationship between hyporheic flow and biogeochemical processing. We propose an alternative conceptual model that hyporheic flow is non-linearly related with biogeochemical processing. Based on the different solute mass transfer and area available for colonization among hydraulic conductivities, we hypothesize that biogeochemical processing in the hyporheic zone follows a Gaussian function depending on hyporheic hydraulic conductivity. After presenting the conceptual model and its domain of application, we discuss the potential implications, notably for river restoration and further hyporheic research.