Molecular characterization of pathogenic group B streptococcus from a tertiary hospital in Shanxi, China: High incidence of sequence type 10 strains in infants/pregnant women.

BACKGROUND: Group B streptococcus (GBS) is a leading cause of serious infection in infants. Understanding its regional molecular epidemiology is helpful for regulating efficient prevention practice. METHODS: A retrospective study was conducted to collected data from infants and pregnant women with culture-proven GBS disease in the largest women and children's medical center in Shanxi between January 2017 and September 2019. All GBS isolates were analyzed by multi-locus sequence typing (MLST) as well as distribution of pilus island (PI) genes. RESULTS: A total of 54 GBS isolates were obtained from 36 (66.7%) pregnant women and 18 (33.3%) infants with invasive disease. Among invasive GBS strains, the most common sequence type was ST10 (72.2%, P < 0.05), followed by ST23 and ST19. The ST10 strain was also the leading sequence type in colonizing pregnant women (44.4%, P < 0.05). All of the isolates carried at least one pilus island. The most frequently detected pilus island was PI-1+PI-2a (85.2%, P < 0.05), followed in turn by PI-2a and PI-2b. CONCLUSIONS: Our study demonstrates that one hypervirulent clone, sequence type 10, accounts for a large proportion of invasive GBS disease in infants and colonizing pregnant women, and the PI-1+PI-2a sub-lineages should be noted in infant infections.

[Impact of changes in land use and climate on the runoff in Liuxihe Watershed based on SWAT model].

SWAT model, an extensively used distributed hydrological model, was used to quantitatively analyze the influences of changes in land use and climate on the runoff at watershed scale. Liuxihe Watershed' s SWAT model was established and three scenarios were set. The calibration and validation at three hydrological stations of Wenquan, Taipingchang and Nangang showed that the three factors of Wenquan station just only reached the standard in validated period, and the other two stations had relative error (RE) < 15%, correlation coefficient (R2) > 0.8 and Nash-Sutcliffe efficiency valve (Ens) > 0.75, suggesting that SWAT model was appropriate for simulating runoff response to land use change and climate variability in Liuxihe watershed. According to the integrated scenario simulation, the annual runoff increased by 11.23 m3 x s(-1) from 2001 to 2010 compared with the baseline period from 1991 to 2000, among which, the land use change caused an annual runoff reduction of 0.62 m3 x s(-1), whereas climate variability caused an annual runoff increase of 11.85 m3 x s(-1). Apparently, the impact of climate variability was stronger than that of land use change. On the other hand, the scenario simulation of extreme land use showed that compared with the land use in 2000, the annual runoff of the farmland scenario and the grassland scenario increased by 2.7% and 0.5% respectively, while that of the forest land scenario were reduced by 0.7%, which suggested that forest land had an ability of diversion closure. Furthermore, the scenario simulation of climatic variability indicated that the change of river runoff correlated positively with precipitation change (increase of 11.6% in annual runoff with increase of 10% in annual precipitation) , but negatively with air temperature change (reduction of 0.8% in annual runoff with increase of 1 degrees C in annual mean air temperature), which showed that the impact of precipitation variability was stronger than that of air temperature change. Therefore, in face of climate variability, we need to pay attention to strong rainfall forecasts, optimization of land use structure and spatial distribution, which could reduce the negative hydrological effects (such as floods) induced by climate change.

[The forensic applications of sex chromosomes and mtDNA genetic markers].

This article summarizes the structure and heredity characteristics of sex chromosomes and mtDNA, presents the advantages and disadvantages of genetic markers' on them in the practice of forensic medicine which aims at promoting more widely using of the non-euchromosome DNA genetic markers in the forensic science.