Madagascar Terrestrial Camera Survey Database 2021: A collation of protected forest camera surveys from 2007-2021.

Madagascar is a threatened global biodiversity hotspot and conservation priority, yet we lack broad-scale surveys to assess biodiversity across space and time. To fill this gap, we collated camera trap surveys, capturing species occurrences within Madagascar into a single standardized database. This data set includes nine distinct protected areas of Madagascar and encompasses 13 subprojects, 38 camera arrays, and 1156 sampling units (independent camera site per survey) within two important biodiversity eco-regions: western dry deciduous forest and eastern humid rainforest. Camera surveys were conducted from June 2007 to January 2021. The final data set includes 17 unique families of mammals (Bovidae, Canidae, Cheirogaleidae, Daubentoniidae, Equidae, Eupleridae, Felidae, Hominidae, Indriidae, Lemuridae, Lepilemuridae, Muridae, Nesomyidae, Pteropodidae, Soricidae, Suidae, Tenrecidae) comprising 45 species and 27 unique families of birds (Accipitridae, Acrocephalidae, Alcedinidae, Bernieridae, Brachypteraciidae, Caprimulgidae, Cisticolidae, Columbidae, Coraciidae, Corvidae, Cuculidae, Dicruridae, Mesitornithidae, Monarchidae, Motacillidae, Muscicapidae, Numididae, Phasianidae, Rallidae, Sarothruridae, Strigidae, Sturnidae, Sulidae, Threskiornithidae, Upupidae, Vangidae, Zosteropidae) comprising 58 species. Images were processed and verified by individual project data set creators and camera operation and species tables were then collated. The final product represents the first broad-scale freely available standardized formal faunal database for Madagascar. Data are available through this publication and at DOI: 10.5281/zenodo.5801806. These data will be useful for examining species-level and community-level trends in occurrence across space or time within Madagascar and globally, evaluating native and invasive species dynamics, and will aid in determining species conservation status and planning for at-risk species. There are no copyright restrictions; please cite this paper when using the data for publication.

Retaliatory killing and human perceptions of Madagascar's largest carnivore and livestock predator, the fosa (Cryptoprocta ferox).

Fosas (Cryptoprocta ferox) are Madagascar's largest carnivores, occupying much of the island's forested landscape. This study provides the first evaluation of fosas' conflict with humans, a problem for many small and medium sized carnivores worldwide. We examined fosas' predation of poultry, and the subsequent retaliatory killing. Over 1750 households were interviewed across four regions, encompassing Madagascar's major forest types (deciduous/rainforest) and protected area classifications (national park, reserve and unprotected forest). Predation by fosa was the third highest reported cause (15%) of poultry mortality, with little evidence that coops were effective in reducing predation. Predation of poultry was more prevalent in deciduous forests, and most common during the evenings of the dry season. Over half of all interviewees said they disliked fosas, with loss of poultry the most commonly stated reason. Respondents' that had suffered poultry depredation and those with lower educational attainment were more likely to dislike fosas. Interviewees that disliked fosas and those that were wealthier were most likely to report having killed a fosa. A minimum of thirty fosas was killed in retaliation by our respondents during the year before the interviews. Given that the fosa population is in decline, and most of Madagascar's forests are likely to be too small to support sustainable populations, these killings may be detrimental to vulnerable sub-populations. These results shed insight into the cultural perceptions and predation patterns of a medium sized carnivore, with relevance to worldwide human-wildlife conflict of often overlooked smaller carnivores. We suggest that educational programs, guard dogs, poultry disease vaccinations and robust coop construction may be effective for improving attitudes and reducing retaliatory killing.

Variation in the hindgut microbial communities of the Florida manatee, Trichechus manatus latirostris over winter in Crystal River, Florida.

The Florida manatee, Trichechus manatus latirostris, is a hindgut-fermenting herbivore. In winter, manatees migrate to warm water overwintering sites where they undergo dietary shifts and may suffer from cold-induced stress. Given these seasonally induced changes in diet, the present study aimed to examine variation in the hindgut bacterial communities of wild manatees overwintering at Crystal River, west Florida. Faeces were sampled from 36 manatees of known sex and body size in early winter when manatees were newly arrived and then in mid-winter and late winter when diet had probably changed and environmental stress may have increased. Concentrations of faecal cortisol metabolite, an indicator of a stress response, were measured by enzyme immunoassay. Using 454-pyrosequencing, 2027 bacterial operational taxonomic units were identified in manatee faeces following amplicon pyrosequencing of the 16S rRNA gene V3/V4 region. Classified sequences were assigned to eight previously described bacterial phyla; only 0.36% of sequences could not be classified to phylum level. Five core phyla were identified in all samples. The majority (96.8%) of sequences were classified as Firmicutes (77.3 ± 11.1% of total sequences) or Bacteroidetes (19.5 ± 10.6%). Alpha-diversity measures trended towards higher diversity of hindgut microbiota in manatees in mid-winter compared to early and late winter. Beta-diversity measures, analysed through PERMANOVA, also indicated significant differences in bacterial communities based on the season.