Bone histomorphometry of the clavicle in a forensic sample from Albania.

Forensic assessment of skeletal material includes age estimation of unknown individuals. When dealing with extremely fragmented human remains that lack macro-features used in age estimation, histological assessment of the skeletal elements can be employed. Historically, microscopic methods for age assessment used by forensic anthropologists have been available since 1965. Several skeletal elements have been used for this purpose. Among them, the clavicle has garnered very little attention. The purpose of this study is to explore the validity of clavicular histomorphometry as an age marker in a modern Balkan sample. This study examined a modern clavicular autopsy sample from Albania. The sample consisted of 33 individuals of known age and cause of death. Data were collected for micro-anatomical features including osteon population density (OPD) and cortical area. Intra- and inter-observer errors were assessed through technical error of measurement (TEM) and R coefficient. A validation study was performed in order to test the accuracy of existing histological formulae. Regression analysis was run to developed age prediction models with the best models tested through cross-validation and the comparison between OPD for the Albanian sample and a European-American sample examined. Intra- and inter-observer error TEM results demonstrated values falling within the limits of acceptance. The existing histological methods did not perform accurately on the sample under study. Regression equations for Albanians produced age estimations deviating 8 and 11 years from known age. Cross-validation on the most accurate regression formula which includes OPD as a single variable demonstrated similar mean errors. Statistically significant differences were observed between the Albanian and the European-American population when the two samples were compared. The research presented is the fifth article published and the fifth population explored on clavicular microstructure. The potential of histology to estimate age on the Albanian population is shown here; however, population effect, diet and health status might be considered. Further inclusion of individuals will corroborate our preliminary findings.

The auricular surface as age indicator in a modern Greek sample: A test of two qualitative methods.

The auricular surface is often found very well preserved, thus age-related changes in this anatomical area can be important for any set of human remains that require identification under different taphonomic conditions. This study tests the Buckberry and Chamberlain (2002) and Schmitt et al. (2005) methods in predicting the age of individuals in a documented sample from Crete, Greece. Both methods were used to record changes on the auricular surface in a mixed-sex sample of 74 individuals, directly as well as through photographs, by two independent observers. Cohen's kappa and intra class correlation coefficients (ICC) were used in order to assess inter-observer and intra-observer agreement. Results showed than none of the methods predicted age with sufficient accuracy, as high error rates were recorded. The Schmitt et al. (2005) method performed better, mainly because the age ranges it uses are broader. Scoring through photographs does not seem to introduce bias in predicting age, as demonstrated by the high intra-observer agreement rates. Inter-observer agreement was also high. The low intra- and inter-observer error rates suggest that the poor performance of both methods in the Cretan sample is not due to a lack of clarity in the description of the morphological changes recorded on the auricular surface; rather it should be attributed to a poor correlation between these changes and age at death in our material.

Sexual dimorphism of the calcaneus in contemporary Cretans.

During the past decade, several studies have been carried out using the calcaneus bone for sex estimation. This paper collected data using ten variables for metric characteristics of the calcaneus of 144 modern Cretans and examined their correlation with known sex. Secondly, the formulae developed by Peckmann et al. (2015) for modern Athenians was put to the test in order to investigate if it could be applied to this modern Cretan sample as well. Results showed a high correlation between the calcaneus metrics and the known sex of the individuals, however the formulae for Athenians do not seem to be suitable for the Cretans due to the high sex bias reported in this study. Thus, new standards were created for sex estimation from the calacanei in our sample. Bilateral asymmetry was noted in the majority of cases, thus formulae were developed for left, right and mean values. Maximum width (MAXW) was the variable that performed the best in the Cretan sample. Overall, the cross-validated accuracies for univariate and multivariate equations reached 84.2% with males most often correctly identified. The calcaneus was proved to be useful for sex estimation in this modern Cretan population. Further work will explore the suitability of the produced standards for other regions of mainland Greece and islands.