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Am J Trop Med Hyg ; 57(6): 719-22, 1997 Dec.
Article in English | MEDLINE | ID: mdl-9430534

ABSTRACT

Genotyping of hepatitis C virus (HCV) of liver disease patients in the Dominican Republic was performed. Eighty-four samples positive for HCV antibody, which were confirmed by ELISA, particle agglutination, and recombinant immunoblot assay III tests, were subjected to HCV genotyping by polymerase chain reaction using type-specific primers located in the nonstructural protein 5 region. Of the 84 samples tested, 50 (59%) were found to have genotype 1a/I and this genotype was the most frequent type detected in the present study. The numbers of isolates of genotypes 1b/II, 2a/III, 2b/IV, and 3a/V were three (3.6%) six (7.1%), two (2.4%), and two 2.4%), respectively. The number of samples having mixed genotype populations was 16 (19%). The possible causes of the high prevalence of genotype 1a/I in the Dominican Republic compared with other countries and of the high detection ratio of samples having mixed genotypes are discussed.


Subject(s)
DNA, Complementary/analysis , Hepatitis C Antibodies/analysis , Hepatitis C/genetics , RNA, Viral/genetics , Viral Nonstructural Proteins/genetics , Agglutination Tests , Dominican Republic/epidemiology , Enzyme-Linked Immunosorbent Assay , Female , Genotype , Hepacivirus/genetics , Hepacivirus/immunology , Hepatitis C/epidemiology , Hepatitis C/immunology , Humans , Immunoblotting , Liver Diseases/diagnosis , Liver Diseases/epidemiology , Liver Diseases/virology , Male , Polymerase Chain Reaction , Prevalence , RNA, Viral/analysis , RNA, Viral/isolation & purification , Recombinant Proteins/immunology , Sensitivity and Specificity
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