ABSTRACT
Petaurus breviceps and Petaurus norfolcensis have produced hybrids in captivity, however there are no reported cases of Petaurus hybridisation in the wild. This study uses morphological data, mitochondrial DNA, and nuclear genome-wide SNP markers to confirm P. breviceps breviceps × P. norfolcensis hybridisation within their natural range on the central coast of New South Wales, Australia. Morphological data identified a potential hybrid that was confirmed with next-generation sequencing technology and 10,111 genome-wide SNPs. Both STRUCTURE and NewHybrid analyses identified the hybrid as a P. norfolcensis backcross, which suggests an initial F1 hybrid was fertile. The mitochondrial DNA matched that of a P. b. breviceps, indicating that a P. b. breviceps female initially mated with a P. norfolcensis male to produce a fertile female offspring. Our study is an important example of how genome-wide SNPs can be used to identify hybrids where the distribution of congeners overlaps. Hybridisation between congeners is likely to become more frequent as climate changes and habitats fragment, resulting in increased interactions and competition for resources and mates.
Subject(s)
Marsupialia , AnimalsABSTRACT
The codling moth (CM) (Cydia pomonella L.) is the most important apple pest in Croatia and Europe. Owing to its economic importance, it is a highly controlled species and the intense selection pressure the species is under has likely caused it to change its phenotype in response. Intensive application of chemical-based insecticide treatments for the control of CM has led to resistance development. In this study, the forewing morphologies of 294 CM (11 populations) were investigated using geometric morphometric procedures based on the venation patterns of 18 landmarks. Finite element method (FEM) was also used to further investigate the dispersal capabilities of moths by modelling wing deformation versus wind speed. Three treatments were investigated and comprised populations from integrated and ecological (susceptible) orchards and laboratory-reared non-resistant populations. Forewing shape differences were found among the three treatment populations investigated. Across all three population treatments, the movement of landmarks 1, 7, 8, 9, and 12 drove the wing shape differences found. A reliable pattern of differences in forewing shape as related to control practice type was observed. FEM revealed that as wind speed (m/s-1) increased, so too did wing deformation (mm) for CM from each of the three treatments modelled. CM from the ecological orchards displayed the least deformation followed by integrated then laboratory-reared CM, which had the highest wing deformation at the highest wind speeds. This study presents an affordable and accessible technique that reliably demonstrates wing shape differences, and thus its use as a population biomarker to detect resistance should be further investigated.