Mycoplasma genitalium and sexually transmitted infections: evidences and figures in a tertiary hospital.

OBJECTIVE: Mycoplasma genitalium is an emerging cause of sexually transmitted infections (STIs) and has been implicated in non-gonococcal urethritis in men and cervicitis in woman. The aim of this study is determinate the incidence and pathogenicity of M. genitalium within the diagnosis of STIs detected from clinical samples in a third level hospital. METHODS: A total of 8,473 samples from endocervix, urethra, vagina, rectum and others were processed applying Allpex STI Essential Assay. More than 190 records were reviewed to determinate M. genitalium pathogenicity. RESULTS: M. genitalium was detected in a rate 2.8%. Co-infections were detected in 20% of the patients. CONCLUSIONS: M. genitalium is considered a STI emerging pathogen thanks to the renewal of multiplex-PCR tests although with a low incidence in our approach. Emerging from our experience and the institutional recommendations both detection of acid nucleic techniques (NAATs) and gonococcal culture might be implemented accurately and coexist to adequate prescriptions.

Staphylococcus lugdunensis Skin Infection: Report of 16 Cases. / Infección cutánea por Staphylococcus lugdunensis: presentación de 16 casos.

INTRODUCTION AND OBJECTIVE: Staphylococcus lugdunensis belongs to the group of coagulase-negative staphylococci. The aim of this report was to review the clinical and microbiologic features of cases of S. lugdunensis skin infections. MATERIAL AND METHODS: Observational study of all cases of skin infections in which S. lugdunensis was isolated by the microbiology department of Hospital General San Jorge in Huesca, Spain, between 2009 and 2016. RESULTS: We studied the cases of 16 patients. The most frequent site of infection was the inguinal-perineal region (n=6, 37.5%), and pustules were the most common presentation (n=5, 31.3%). Response to treatment was good in 87.6% of the patients (n=14). However, infection recurred in 3 patients, 2 of whom were on anti-TNF therapy. CONCLUSIONS: S. lugdunensis should be considered a possible cause of infection when it is isolated in both skin and subcutaneous tissues, especially in patients on biologic therapies.

Mycobacterium fortuitum infection after acupuncture treatment.

Actinic keratoses are cutaneous lesions that appear as the result of the proliferation of atypical keratinocytes. These lesions are considered pre-malignant and they can progress to squamous cell carcinoma. Ingenol mebutate has been approved as an effective treatment for AK on the face and trunk. We studied the local skin reactions to this therapy. Data about local skin reactions were collected in a series of 5 patients with photographic documentation, a visual analog scale, and a ranking of satisfaction of the patient. Moderate to severe reactions were reported in most of patients, but only one stopped treatment early. The short duration of treatment contributes to high adherence to the therapy.

Paciente con coloración cutaenomucosa anaranjada y dolor abdominal inespecífico / Patient with orange muco-cutaneous discoloration and nonspecific abdominal pain

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[UTIscreen versus UROQUICK: two semiautomatic systems for bacteriuria detection]. / UTIscreen frente a UROQUICK: dos sistemas semiautomáticos de detección de bacteriuria.

BACKGROUND: The aim of the study is to find a rapid and reliable method for urine screen. With this purpose two semiautomatic systems have been evaluated: UTIscreen and UROQUICK. MATERIAL AND METHODS: We have carried out a prospective study of a total 1,070 urine specimens from patients with suspected urinary infection. Each sample has been inoculated in the two semiautomatic systems and compared with the semiquantitative plate culture as the reference method. RESULTS: Both semiautomatic systems showed similar specificity and sensitivity at an interpretative breakpoint of 30,000 CFU/ml, with a low number of false positives. Uninfected specimens can be reported the same day of reception. CONCLUSIONS: Both UTIscreen and UROQUICK systems seem to be adequate for urinary tract infection screening. They are reliable and easy to perform, but in our particular case we find advantage in using UTIscreen because the faster reading of this system makes posible to report the negative results in the same morning of reception of the specimen and do the culture of all the positive samples during our usual working schedule.

[Incidence of Cryptosporidium in Zaragoza: an 8-year study (1989-1996)]. / Incidencia de Cryptosporidium en Zaragoza: estudio de 8 años (1989-1996).

BACKGROUND: To study the incidence of Cryptosporidium infections over an eight year period in an urban area, together with the patients background and the season of the year. MATERIALS AND METHODS: From January 1989 to December 1996, all 32,733 stool samples from 13,639 patients: children and immunocompromised adults, with presumed infective diarrhoea, were investigated for Cryptosporidium oocysts by the Department of Microbiology, Miguel Servet Hospital, Zaragoza (Spain). RESULTS: Cryptosporidial infection was identified in a 3% of the total children, been the positivity rate highest in the 2-year old group. We visualized oocysts in the 8% of the immunocompromised patients, all of them HIV-positive. The prevalence was higher in winter (February-March). CONCLUSIONS: According to these findings we conclude that Cryptosporidium should be systematically searched by clinical laboratories, specially in children and in immunocompromised patients suffering from infectious diarrhoea.