ABSTRACT
Heat production (HP) represents a major energy cost in lactating dairy cows. Better understanding of factors that affect HP will improve our understanding of energy metabolism. Our objective was to derive models to explain variation in HP of lactating Jersey cows. Individual animal-period data from 9 studies (n = 293) were used. The data set included cows with a wide range (min to max) in days in milk (44-410) and milk yield (7.8-43.0 kg/d). Diets included corn silage as the predominate forage source, but diets varied (min to max on DM basis) in crude protein (CP; 15.2-19.5%), neutral detergent fiber (NDF; 35.5-43.0%), starch (16.2-31.1%), and crude fat (2.2 to 6.4%) contents. Average HP was (mean ± standard deviation) 22.1 ± 2.86 Mcal/d, or 28.1 ± 3.70% of gross energy intake. Eight models were fit to explain variation in HP: (1) dry matter intake (DMI; INT); (2) milk fat, protein, and lactose yield (MILKCOMP); (3) INT and milk yield (INT+MY); (4) INT and MILKCOMP/DMI (INT+MILKCOMP); (5) mass of digested NDF, CP, and starch (DIG); (6) INT and digested energy (INT+DE); (7) INT and NDF, CP, and starch digestibility (INT+DIG); or (8) INT+MILKCOMP model plus urinary N excretion (INT+MILKCOMP+UN). For all HP models, metabolic body weight was included. All models were derived via a backward elimination approach and included the random effects of study, cow, and period within block within study. The INT models adequately explained variation in HP with a nonrandom effect-adjusted concordance correlation coefficient of 0.84. Similar adjusted concordance correlation coefficients (0.79-0.85) were observed for other HP models. The HP associated with milk protein yield and supply of digestible protein was greater than other milk production and nutrient digestibility variables. The HP associated with urinary N excretion was 5.32. Overall, HP can be adequately predicted from metabolic body weight and DMI. Milk component yield, nutrient digestibility, or urinary N excretion explained similar variation as DMI. Coefficients for milk protein and protein digestion suggest that digestion and metabolism of protein and synthesis of milk protein contribute substantially to HP of a dairy cow.
Subject(s)
Cattle/physiology , Diet/veterinary , Lactation/physiology , Thermogenesis/physiology , Animals , Dietary Fiber/administration & dosage , Dietary Proteins/administration & dosage , Digestion , Energy Intake , Energy Metabolism/physiology , Female , Lactose/analysis , Milk/chemistry , Milk Proteins/analysis , Silage/analysis , Zea mays/metabolismABSTRACT
Millions of feed composition records generated annually by testing laboratories are valuable assets that can be used to benefit the animal nutrition community. However, it is challenging to manage, handle, and process feed composition data that originate from multiple sources, lack standardized feed names, and contain outliers. Efficient methods that consolidate and screen such data are needed to develop feed composition databases with accurate means and standard deviations (SD). Considering the interest of the animal science community in data management and the importance of feed composition tables for the animal industry, the objective was to develop a set of procedures to construct accurate feed composition tables from large data sets. A published statistical procedure, designed to screen feed composition data, was employed, modified, and programmed to operate using Python and SAS. The 2.76 million data received from 4 commercial feed testing laboratories were used to develop procedures and to construct tables summarizing feed composition. Briefly, feed names and nutrients across laboratories were standardized, and erroneous and duplicated records were removed. Histogram, univariate, and principal component analyses were used to identify and remove outliers having key nutrients outside of the mean ± 3.5 SD. Clustering procedures identified subgroups of feeds within a large data set. Aside from the clustering step that was programmed in Python to automatically execute in SAS, all steps were programmed and automatically conducted using Python followed by a manual evaluation of the resulting mean Pearson correlation matrices of clusters. The input data set contained 42, 94, 162, and 270 feeds from 4 laboratories and comprised 25 to 30 nutrients. The final database included 174 feeds and 1.48 million records. The developed procedures effectively classified by-products (e.g., distillers grains and solubles as low or high fat), forages (e.g., legume or grass-legume mixture by maturity), and oilseeds versus meal (e.g., soybeans as whole raw seeds vs. soybean meal expellers or solvent extracted) into distinct sub-populations. Results from these analyses suggest that the procedure can provide a robust tool to construct and update large feed data sets. This approach can also be used by commercial laboratories, feed manufacturers, animal producers, and other professionals to process feed composition data sets and update feed libraries.
Subject(s)
Animal Feed/analysis , Diet/veterinary , Livestock , Animal Feed/classification , Animals , Cluster Analysis , Databases, Factual , Dietary Carbohydrates/analysis , Dietary Carbohydrates/classification , Dietary Fats/analysis , Dietary Fats/classification , Dietary Fiber/analysis , Dietary Fiber/classification , Dietary Proteins/analysis , Dietary Proteins/classification , Fabaceae/chemistry , Glutens/analysis , Glycine max/chemistry , Zea mays/chemistryABSTRACT
Although the inclusion of fat has reduced methane production in ruminants, relatively little research has been conducted comparing the effects of source and profile of fatty acids on methane production in lactating dairy cows. A study using 8 multiparous (325 ± 17 DIM; mean ± SD) lactating Jersey cows was conducted to determine effects of feeding canola meal and lard versus extruded byproduct containing flaxseed as a high-C18:3 fat source on methane production and diet digestibility in late-lactation dairy cows. A crossover design with 32-d periods (28-d adaptation and 4-d collections) was used to compare 2 different fat sources. Diets contained approximately 50% forage mixture of corn silage, alfalfa hay, and brome hay; the concentrate mixture changed between diets to include either (1) a conventional diet of corn, soybean meal, and canola meal with lard (control) or (2) a conventional diet of corn and soybean meal with an extruded byproduct containing flaxseed (EXF) as the fat source. Diets were balanced to decrease corn, lard, and canola meal and replace them with soybean mean and EXF to increase the concentration of C18:3 (0.14 vs. 1.20% of DM). Methane production was measured using headbox-style indirect calorimeters. Cattle were restricted to 95% ad libitum feed intake during collections. Milk production (17.4 ± 1.04 kg/d) and dry matter intake (15.4 ± 0.71 kg/d) were similar among treatments. Milk fat (5.88 ± 0.25%) and protein (4.08 ± 0.14%) were not affected by treatment. For methane production, no difference was observed for total production (352.0 vs. 349.8 ± 16.43 L/d for control vs. EXF, respectively). Methane production per unit of dry matter intake was not affected and averaged 23.1 ± 0.57 L/kg. Similarly, methane production per unit of energy-corrected milk was not affected by fat source and averaged 15.5 ± 0.68 L/kg. Heat production was similar, averaging 21.1 ± 1.02 Mcal/d. Digestibility of organic matter, neutral detergent fiber, and crude protein was not affected by diet and averaged 69.9, 53.6, and 73.3%, respectively. Results indicated that increasing C18:3 may not affect methane production or digestibility of the diet in lactating dairy cows.
Subject(s)
Animal Feed , Diet/veterinary , Lactation/drug effects , Methane/biosynthesis , alpha-Linolenic Acid/pharmacology , Animals , Cattle , Dietary Fiber/metabolism , Dietary Supplements , Fatty Acids/metabolism , Female , Milk/chemistry , Silage/analysis , Glycine max/metabolism , Zea mays/metabolismABSTRACT
The addition of fat and calcium sulfate to diets fed to ruminants has resulted in a reduction in methane production, but the effects on energy balance have not been studied. A study using indirect calorimetry and 16 multiparous (8 Holstein and 8 Jersey; 78 ± 15 d in milk; mean ± standard deviation) lactating dairy cows was conducted to determine how mitigating methane production by adding corn oil or calcium sulfate to diets containing reduced-fat distillers grains affects energy and nitrogen balance. A replicated 4 × 4 Latin square design with 35-d periods (28 d of adaption and 4 d of collections) was used to compare 4 different dietary treatments. Treatments were composed of a control (CON) diet, which did not contain reduced-fat distillers grain and solubles (DDGS), and treatment diets containing 20% (dry matter basis) DDGS (DG), 20% DDGS with 1.38% (dry matter basis) added corn oil (CO), and 20% DDGS with 0.93% (dry matter basis) added calcium sulfate (CaS). Compared with CON, dry matter intake was not affected by treatment, averaging 29.6 ± 0.67 kg/d. Milk production was increased for diets containing DDGS compared with CON (26.3 vs. 27.8 ± 0.47 kg/d for CON vs. DDGS, respectively), likely supported by increased energy intake. Compared with CON, energy-corrected milk was greater in DG and CO (30.1 vs. 31.4, 31.7, and 31.0 ± 0.67 kg/d for CON, DG, CO, and CaS, respectively). Compared with CON, the addition of calcium sulfate and corn oil to diets containing DDGS reduced methane production per kg of dry matter intake (22.3, 19.9, and 19.6 ± 0.75 L/kg per d for CON, CO, and CaS, respectively). Similarly, methane production per kilogram of energy-corrected milk was reduced with the addition of calcium sulfate and corn oil to diets containing DDGS (14.2, 12.5, and 12.4 ± 0.50 L/kg per d for CON, CO, and CaS, respectively). Compared with CON and CaS, the intake of digestible energy was greater for DG and CO treatments (57.7, 62.1, 62.0, and 59.0 ± 1.38 Mcal/d for CON, DG, CO, and CaS, respectively). Intake of metabolizable energy was greater in all treatments containing DDGS compared with CON (50.5 vs. 54.0 ± 1.08 Mcal/d for CON vs. DDGS, respectively). Net balance (milk plus tissue energy) per unit of dry matter was greater in CO (containing DDGS and oil) than CON (1.55 vs. 1.35 ± 0.06 Mcal/kg for CO vs. CON, respectively). Tissue energy was greater in DG and CO compared with CON (6.08, 7.04, and 3.16 ± 0.99 Mcal/d for DG, CO, and CON, respectively. Results of this study suggest that the addition of oil and calcium sulfate to diets containing DDGS may be a viable option to reduce methane production and in the case of oil also improve net energy balance in lactating dairy cows.
Subject(s)
Calcium Sulfate/metabolism , Cattle/physiology , Corn Oil/metabolism , Energy Metabolism , Methane/metabolism , Nitrogen/analysis , Animal Feed/analysis , Animals , Calcium Sulfate/administration & dosage , Corn Oil/administration & dosage , Dairying , Diet/veterinary , Dietary Supplements/analysis , Energy Intake , Female , Random AllocationABSTRACT
Methane (CH4) production of ruminants typically increases with increased dry matter intake (DMI). However, few studies have observed the effects of feeding multiple times a day and its effects on diurnal variation in CH4 production and energy balance in late-lactation dairy cattle. A study using headbox-style indirect calorimetry and 12 multiparous (225 ± 16.2 d in milk; mean ± SD) lactating Jersey cows was conducted to determine the effects of feeding twice daily on diurnal variation in CH4 production and total energy balance. A crossover design with 14-d periods (10 d of adaption and 4 d of collection) was used to compare 2 treatments. Treatments consisted of either once a day feeding (1×; 100% of feed given at 1000 h) or twice a day feeding (2×; 50% of feed given at 1000 h and the final 50% at 2000 h) with a common diet fed in both treatments. Dry matter intake was not different between treatments, with a mean of 16.9 ± 0.88 kg/d. Once a day feeding tended to have greater milk yield compared with twice a day feeding (21.2 vs. 20.4 ± 1.59 kg/d, respectively). Milk fat and milk protein percentage were not different, with means of 6.18 ± 0.20% and 3.98 ± 0.08%, respectively. Total CH4 production did not differ between treatments, with a mean of 402.1 ± 20.8 L/d. Similarly, CH4 per unit of milk yield and DMI was not different between treatments, with means of 20.5 ± 1.81 and 23.8 ± 1.21 L/kg, respectively. Feeding frequency did not affect diurnal variation of hourly CH4 production, with a mean of 17.1 ± 0.74 L/h. A trend was observed for a treatment × hour interaction. Methane production per hour increased after the second feeding for cattle fed twice versus once daily. Gross energy, digestible energy, metabolizable energy, and balance (milk plus tissue) per kilogram of DMI did not differ by feeding frequency, with means of 4.41 ± 0.01, 3.05 ± 0.03, 2.63 ± 0.03, and 1.32 ± 0.08 Mcal/kg of DM, respectively. Metabolizable energy for maintenance was 146 kcal/kg of metabolic body weight, with an efficiency of converting metabolizable energy to net energy balance (milk plus tissue) of 76%. Nitrogen balance did not differ among treatments, with a mean balance of 17.3 ± 13.0 g/d. Therefore, total CH4 production and energy maintenance were not affected by feeding frequency. However, CH4 was variable throughout the day, and caution should be exercised when collecting CH4 samples at a limited number of time points because this may under- or overestimate total production.
Subject(s)
Circadian Rhythm , Dairying/methods , Feeding Behavior , Lactation , Methane/biosynthesis , Animals , Body Weight , Cattle , Diet/veterinary , Energy Metabolism , Female , Milk , Milk Proteins/analysisABSTRACT
Feeding fat to lactating dairy cows may reduce methane production. Relative to cellulose, fermentation of hemicellulose is believed to result in less methane; however, these factors have not been studied simultaneously. Eight multiparous, lactating Jersey cows averaging (±SD) 98 ± 30.8 d in milk and body weight of 439.3 ± 56.7 kg were used in a twice-replicated 4 × 4 Latin square to determine the effects of fat and hemicellulose on energy utilization and methane production using a headbox-type indirect calorimetry method. To manipulate the concentration of fat, porcine tallow was included at either 0 or 2% of the diet dry matter. The concentration of hemicellulose was adjusted by manipulating the inclusion rate of corn silage, alfalfa hay, and soybean hulls resulting in either 11.3 or 12.7% hemicellulose (dry matter basis). The resulting factorial arrangement of treatments were low fat low hemicellulose (LFLH), low fat high hemicellulose (LFHH), high fat low hemicellulose (HFLH), and high fat high hemicellulose (HFHH). Neither fat nor hemicellulose affected dry matter intake, averaging 16.2 ± 1.18 kg/d across treatments. Likewise, treatments did not affect milk production, averaging 23.0 ± 1.72 kg/d, or energy-corrected milk, averaging 30.1 ± 2.41 kg/d. The inclusion of fat tended to reduce methane produced per kilogram of dry matter intake from 24.9 to 23.1 ± 1.59 L/kg, whereas hemicellulose had no effect. Increasing hemicellulose increased neutral detergent fiber (NDF) digestibility from 43.0 to 51.1 ± 2.35%. Similarly, increasing hemicellulose concentration increased total intake of digestible NDF from 6.62 to 8.42 ± 0.89 kg/d, whereas fat had no effect. Methane per unit of digested NDF tended to decrease from 64.8 to 49.2 ± 9.60 L/kg with increasing hemicellulose, whereas fat had no effect. An interaction between hemicellulose and fat content on net energy balance (milk plus tissue energy) was observed. Specifically, increasing hemicellulose in low-fat diets tended to increase net energy balance, but this was not observed in high-fat diets. These results confirm that methane production may be reduced with the inclusion of fat, whereas energy utilization of lactating dairy cows is improved by increasing hemicellulose in low-fat diets.
Subject(s)
Cattle/metabolism , Dietary Fats/metabolism , Energy Metabolism , Methane/biosynthesis , Polysaccharides , Animals , Diet , Digestion , Female , Lactation , Medicago sativa , Milk , Rumen , Silage , Swine , Zea maysABSTRACT
Genetic variants associated with traits such as age at puberty and litter size could provide insight into the underlying genetic sources of variation impacting sow reproductive longevity and productivity. Genomewide characterization and gene expression profiling were used using gilts from the University of Nebraska-Lincoln swine resource population ( = 1,644) to identify genetic variants associated with age at puberty and litter size traits. From all reproductive traits studied, the largest fraction of phenotypic variation explained by the Porcine SNP60 BeadArray was for age at puberty (27.3%). In an evaluation data set, the predictive ability of all SNP from high-ranked 1-Mb windows (1 to 50%), based on genetic variance explained in training, was greater (12.3 to 36.8%) compared with the most informative SNP from these windows (6.5 to 23.7%). In the integrated data set ( = 1,644), the top 1% of the 1-Mb windows explained 6.7% of the genetic variation of age at puberty. One of the high-ranked windows detected (SSC2, 12-12.9 Mb) showed pleiotropic features, affecting both age at puberty and litter size traits. The RNA sequencing of the hypothalami arcuate nucleus uncovered 17 differentially expressed genes (adjusted < 0.05) between gilts that became pubertal early (<155 d of age) and late (>180 d of age). Twelve of the differentially expressed genes are upregulated in the late pubertal gilts. One of these genes is involved in energy homeostasis (), a function in which the arcuate nucleus plays an important contribution, linking nutrition with reproductive development. Energy restriction during the gilt development period delayed age at puberty by 7 d but increased the probability of a sow to produce up to 3 parities ( < 0.05). Identification of pleotropic functional polymorphisms may improve accuracy of genomic prediction while facilitating a reduction in sow replacement rates and addressing welfare concerns.
Subject(s)
Genetic Variation , Genomics , Reproduction/genetics , Sexual Maturation/genetics , Swine/genetics , Animals , Female , Genome-Wide Association Study/veterinary , Litter Size/genetics , Phenotype , Pregnancy , Swine/physiologyABSTRACT
Two experiments were conducted to investigate the effects of collection method and diet type on digestibility coefficients. In Exp. 1, 24 barrows were fed either a corn-soybean meal (CSBM) diet or CSBM with 20% dried distillers' grains with solubles (CSBM-DDGS). In Exp. 2, the effects of basal diet and collection method on determination of dried distillers' grains with solubles (DDGS) digestibility were studied using 24 barrows. The 4 diets used in Exp. 2 were: a CSBM (basal 1) , a barley-canola meal (BCM; basal 2), 80% basal 1 with 20% DDGS (CSBM-DDGS), and 80% basal 2 with 20% DDGS (BCM-DDGS). In both experiments, feces were collected using a time-based collection method (DY) or a "marker-to-marker" collection method (MM). Diets contained 0.5% of titanium dioxide (TiO) for estimating digestibility using the index marker approach (IM). The apparent total tract digestibility (ATTD) of DM and GE were lower ( < 0.05) in the CSBM-DDGS diet than in the CSBM diet in Exp. 1 but were not different in Exp. 2. All the estimates of BCM-based diets were consistently lower ( < 0.05) than those of CSBM-based diets. In Exp. 1, digestibility coefficients determined by the DY and MM were not different from each other, whereas those estimates were lower ( < 0.05) using the IM than those using the total collection approach (TC; DY and MM). In Exp. 2, interactions ( < 0.05) were observed between diet type and method for dietary digestibility coefficients. Digestibility and energy values estimated by the DY and MM were not different in pigs fed CSBM-based diets and the BCM-DDGS diet, whereas those estimates were greater ( < 0.05) using the DY than those using the MM in pigs fed the BCM. There were no interactions between basal diet and method for estimating DDGS digestibility. The ATTD of DM and GE of DDGS using the MM were greater ( < 0.05) than those using the IM, and ATTD of N tended to be greater ( < 0.10) using the MM than that using the IM. All estimates using the DY were not different from those using the MM or the IM, except that DE of DDGS was greater ( < 0.05) using the DY than when using the IM. Digestibility estimates of DDGS were not affected by basal diets. The mean DE and ME (as-fed basis) of DDGS were 3,994 and 3,688 kcal/kg, respectively, when estimated using the basal 1 diet and were 3,919 and 3,547 kcal/kg, respectively, when estimated using the basal 2 diet. In conclusion, both collection methods can be used to estimate energy and nutrient digestibility of diets and DDGS when using CSBM-based diets.
Subject(s)
Swine/physiology , Animal Feed/analysis , Animal Nutritional Physiological Phenomena , Animals , Brassica rapa , Diet/veterinary , Digestion , Feces , Hordeum , Male , Random Allocation , Glycine max , Zea maysABSTRACT
An experiment with 2 trials (28 d/trial) was conducted to determine body composition, tissue deposition, and utilization of Lys for protein deposition (PD) of barrows and gilts fed -Lys·HCl (CLys) or protein-bound Lys in soybean meal (SBM). Thirty-two growing pigs (16 barrows and 16 gilts; average initial BW of 18.6 kg) were used in each of 2 trials. Four pigs (2 barrows and 2 gilts) were euthanized at the start of each trial to determine initial body composition. The remaining pigs were euthanized at the end of the trials to determine empty-body composition and deposition rates of water, protein, fat, ash, and AA. Pigs were randomly allotted to 1 of 7 dietary treatments. There were 2 replications per treatment in each trial for a total of 4 replications. Dietary treatments consisted of a corn-SBM basal diet (0.48% Lys) and diets containing 0.56%, 0.65%, and 0.74% standardized ileal digestible (SID) Lys that were achieved by adding Lys to the basal diet from either SBM or CLys. Pigs fed the CLys-supplemented diets at 0.65% SID Lys had more ( < 0.05) body water (663 vs. 624 g/kg) and less ( < 0.01) body protein (153 vs. 160 g/kg) than pigs fed the SBM-supplemented diets. Body fat content decreased ( < 0.01) as the dietary Lys increased similarly for pigs fed Lys from SBM and pigs fed CLys. Gilts had greater ( = 0.05) body Lys content in body protein than barrows (7.68 vs. 7.52 g/100 g). Empty-body ash contents were not different between pigs fed CLys or SBM-supplemented diets. Water deposition and PD increased linearly ( < 0.01) with dietary Lys and were least ( < 0.01) in pigs fed the basal diet but were similar when comparing pigs fed CLys and SBM-supplemented diets at the same dietary Lys concentration. Lysine deposition showed a linear increase ( < 0.01) with dietary Lys but was not different between pigs fed the 2 Lys sources at the same concentration. Barrows and gilts did not differ in tissue deposition rates. Overall, empty-body contents and deposition rates of essential and nonessential AA were not different between pigs fed CLys and pigs fed SBM-bound Lys. The amount of SID Lys required for PD ranged between 0.09 and 0.13 g/g for both sources of Lys. The Lys deposition:SID Lys intake ratio was greater ( < 0.01) in gilts than barrows (0.62 vs. 0.56). Body composition, tissue deposition, and utilization of Lys for PD and Lys deposition were not different in pigs fed diets supplemented with -Lys·HCl with respect to protein-bound Lys in SBM.
Subject(s)
Animal Feed/analysis , Body Composition/drug effects , Dietary Supplements , Lysine/pharmacology , Swine/physiology , Animal Nutritional Physiological Phenomena , Animals , Diet/veterinary , Female , Ileum/metabolism , Lysine/chemistry , Lysine/metabolism , Male , Glycine max/metabolismABSTRACT
A cooperative study comprising growth performance, bone mineralization, and nutrient balance experiments was conducted at 11 stations to determine the standardized total-tract digestible (STTD) P requirement of 20-kg pigs using broken-line regression analysis. Monocalcium phosphate and limestone were added to a corn-soybean meal-based diet at the expense of cornstarch to establish 6 concentrations of STTD P from 1.54 to 5.15 g/kg in increments of 0.62 g/kg at a constant Ca:total P of 1.52:1.0. Diets were fed to 936 pigs (average initial BW of 19 kg) in 240 pens for 20 replicate pens of barrows and 20 replicate pens of gilts per diet. As STTD P increased from 1.54 to 5.15 g/kg of the diet for d 0 to 14, 14 to 28, and 0 to 28, the ADG, ADFI, and G:F increased ( < 0.01). Barrows gained and ate more ( < 0.05) than gilts during d 14 to 28 and 0 to 28. There was no interaction between sex and STTD P concentration for any of the growth performance response criteria. There were both linear and quadratic increases ( < 0.05) in mineral density and content of ash, Ca, and P in the femur expressed as a percentage of dry, fat-free metacarpal as dietary STTD P increased. Furthermore, the maximum load of the femur and mineral density and content and maximum load as well as the Ca and P expressed as a percentage of metacarpal ash linearly increased ( < 0.01) with increasing dietary concentrations of STTD P. There were both linear and quadratic increases ( < 0.01) in apparent digestibility and retention of P with increasing concentrations of STTD P in the diets. Digestibility and retention of Ca linearly ( < 0.01) increased with increasing dietary concentrations of STTD P. Breakpoints determined from nonlinear broken-line regression analyses revealed estimates of 4.20 ± 0.102, 3.20 ± 0.036, or 3.87 ± 0.090 g/kg for ADG during d 0 to 14, 14 to 28, or 0 to 28, respectively. Corresponding estimates using G:F as the response criterion were 4.34 ± 0.146, 3.38 ± 0.139, or 4.08 ± 0.195 g/kg. When mineralization of the femur was used as criteria of response, estimates of STTD P requirement were 4.28, 4.28, or 4.34, g/kg for mineral density, mineral content, or maximum load, respectively. Using mineralization of the metacarpal as criteria of response, estimates of STTD P requirement ranged from 3.5 to 5.0 g/kg depending on the metacarpal response criteria. The study provided empirical estimates of STTD P requirements of 20- to 40-kg pigs.
Subject(s)
Phosphorus, Dietary/administration & dosage , Swine/physiology , Animal Feed/analysis , Animal Nutritional Physiological Phenomena/physiology , Animals , Calcification, Physiologic , Calcium Carbonate/administration & dosage , Calcium Phosphates/administration & dosage , Diet/veterinary , Female , Femur/physiology , Gastrointestinal Tract/physiology , Male , Metacarpal Bones/physiology , Minerals/administration & dosage , Phosphorus, Dietary/metabolism , Random Allocation , Regression Analysis , Glycine max , Swine/growth & developmentABSTRACT
Eight Holstein and 8 Jersey multiparous, lactating cows were used to complete 56 energy balances to determine the energy content of reduced-fat dried distillers grains with solubles (RFDDGS). A repeated switchback design was used to compare treatments with and without RFDDGS. Diets consisted of 24.2% corn silage, 18.4% alfalfa hay, 6.94% brome hay with either 22.9% rolled corn or 14.8% soybean meal (control), or 8.95% rolled corn, 28.8% RFDDGS, and 0% soybean meal [Co-P; dry-matter (DM) basis]. The inclusion of RFDDGS did not affect DM intake, averaging 21.4 ± 0.53 kg of DM for all cows, but milk production tended to increase from 29.8 to 30.9 ± 1.46 kg/d for control and Co-P treatments, respectively. Milk fat percentage and energy-corrected milk did not differ between treatments, averaging 4.33 ± 0.14% and 34.1 kg/d, respectively. Milk protein was significantly decreased by the Co-P treatment (3.56 and 3.41 ± 0.08% for control and Co-P treatments), but protein yield was not affected. Milk energies were 1.40 Mcal/d greater with Co-P. Energy lost as methane was reduced by 0.31 Mcal/d with the addition of RFDDGS to the diet. Heat loss averaged 29.9 ± 0.55 Mcal/d and was not different between diets. Average energy retained as tissue energy was -2.99 ± 0.93 Mcal/d and did not differ between treatments. Intake of digestible and metabolizable energy were not different between the control and Co-P treatments, averaging 2.68 and 2.31 Mcal/kg of DM, respectively. The net energy of lactation values of control and Co-P diets were calculated to be 1.43 and 1.47 Mcal/kg of DM, respectively. These energy estimates suggest greater energy content of diets containing RFDDGS than diets containing a mixture of corn and soybean meal in lactating dairy cows.
Subject(s)
Animal Feed/analysis , Cattle , Energy Intake , Lactation/physiology , Zea mays , Animals , Diet/veterinary , Energy Metabolism , Fats/analysis , Female , Milk/chemistry , Milk Proteins , Silage , Glycine max , Waste ProductsABSTRACT
Grains grown in various regions of the United States vary in their innate or natural Se contents. A regional study evaluated the effects of adding inorganic Se (sodium selenite) or organic Se (Se yeast) to diets with differing innate Se contents. A 2 × 2 + 1 factorial experiment evaluating 2 Se sources (organic or inorganic) at 2 Se levels (0.15 or 0.30 mg/kg) in 18 total replicates (n = 360 total pigs). A basal diet was fed without supplemental Se and served as the negative (basal) control. The study was conducted as a randomized complete block design in 9 states (Georgia, Illinois, Kentucky, Nebraska, North Carolina, Ohio, South Dakota, Texas, and Wisconsin) with each station conducting 2 replicates. Pigs were fed from 25 to approximately 115 kg BW. Similar dietary formulations were used at each station, incorporating a common source of trace mineral and Se premixes. Three pigs per treatment in 16 replicates (n = 240) were bled at 55, 85, and 115 kg BW and serum Se and glutathione peroxidase (GSH-Px) activities were determined. Three pigs (n = 260) from each treatment pen were killed at 115 kg BW and issues (liver, loin, and hair) were analyzed for Se. The corn Se content from the various states ranged from 0.026 to 0.283 mg Se/kg while the soybean meal Se content ranged from 0.086 to 0.798 mg Se/kg. Tissue and serum Se concentrations were greater (P < 0.01) when supplemental organic Se was fed, whereas serum GSH-Px was greater (P < 0.01) as Se level increased. There were linear increases (P < 0.01) in loin and quadratic increases (P < 0.01) in liver and hair Se concentrations as dietary Se level increased within each state. There was a source × level interaction (P < 0.01) for each tissue resulting in a greater increase when organic Se was fed. Serum Se and GSH-Px activity increased (P < 0.01) when both Se sources were fed and plateaued at each state at 0.15 mg Se/kg. There was a high and significant correlation between each tissue Se, serum Se, and GSH-Px activity to dietary Se level indicating that those states having greater grain natural Se contents also had greater tissue Se concentrations. These results indicate that a large difference in corn and soybean meal Se concentrations exists between states, that the addition of organic or inorganic Se to these grains increased tissue and serum Se in each state, and that organic Se was incorporated at greater concentrations in the loin, liver, and hair tissues of grower-finisher pigs than inorganic Se.
Subject(s)
Dietary Supplements , Edible Grain , Selenium Compounds/pharmacology , Selenium/metabolism , Sodium Selenite/pharmacology , Swine/growth & development , Animals , Diet/veterinary , Edible Grain/chemistry , Glutathione Peroxidase/metabolism , Liver/metabolism , Muscle, Skeletal/metabolism , Selenium/analysis , Selenium Compounds/administration & dosage , Sodium Selenite/administration & dosage , Glycine max/chemistry , Swine/metabolism , United States , Zea mays/chemistryABSTRACT
Four experiments were conducted to evaluate effects of yeast-dried milk (YDM) product in creep and phase-1 nursery diets. In Exp. 1, 24 parity-4 litters were allotted to 3 dietary treatments (8 litters/treatment) including no creep (NC), control creep (CTL), and experimental creep (EC; 10% YDM). Creep diets were fed twice daily from d 7 after birth until weaning (23.6 ± 1.8 d). In Exp. 2, 108 weaned pigs were selected based on mean BW of pigs from the respective treatments in Exp. 1. For phase 1 (d 0 to 7 postweaning) of Exp. 2, NC and CTL pigs were fed the CTL diet and EC pigs continued to receive the EC diet. For phase 2 and 3 (d 7 to 28 postweaning) of Exp. 2, all pigs received a common diet containing antibiotics. Blood and fecal samples were collected on d 0, 7, 14, and 21 postweaning to evaluate serum IgA and fecal microbiota. In Exp. 1, pigs fed EC and CTL tended to have greater (P < 0.10) weaning BW compared with NC pigs. Pigs fed EC had greater (P < 0.05) ADFI compared with CTL pigs. In Exp. 2, pigs fed EC tended to have greater BW (P < 0.10) and greater ADG (P < 0.05) and ADFI (P < 0.01) compared with CTL and NC pigs (d 0 to 28). For serum IgA, EC and CTL pigs tended (P < 0.10) to have greater IgA compared with NC pigs. For microbial data, EC pigs had greater (P < 0.01) microbial diversity compared with CTL (d 7 postweaning). On d 7 and 21 postweaning, microbial similarity decreased (P < 0.01) in pigs fed EC compared with NC and CTL. Overall (d 0 to 14), lactobacilli gene copy numbers tended (P < 0.10) to be greater in EC (7.3 log10) compared with NC pigs (6.9 log10). In Exp. 3, 23 parity-1 litters were allotted to 3 dietary treatments as described for Exp. 1. In Exp. 4, 108 weaned pigs were selected based on mean BW of all pigs from Exp. 3 and dietary treatments were the same as described for Exp. 2 except no antibiotic was included in phase-2 diet. In Exp. 3, there were no treatment effects on litter ADFI, ADG, and serum IgA, but a tendency (P < 0.10) for lower Lactobacillus reuteri in the CTL (5.1 log10) compared with NC pigs (5.6 log10) was observed. Overall (d 0 to 21) in Exp. 4, pigs fed EC had greater ADG (P < 0.01) and G:F (P < 0.05) and tended to exhibit greater ADFI (P < 0.10) compared with the CTL. For microbial data, pigs fed CTL (4.8 log10) and EC (4.8 log10) had lower (P < 0.01) fecal Lactobacillus johnsonii compared with NC (5.2 log10). Microbial ecology and immune parameters are affected by YDM.
Subject(s)
Animal Feed/analysis , Animal Nutritional Physiological Phenomena , Diet/veterinary , Milk/metabolism , Sus scrofa/growth & development , Sus scrofa/microbiology , Yeast, Dried/pharmacology , Animals , Feces/microbiology , Feeding Methods/veterinary , Immunoglobulin A/blood , Microbiota , Sus scrofa/immunology , Swine , Yeast, Dried/administration & dosageABSTRACT
Two experiments were conducted to evaluate the effects of spray-dried porcine plasma (SDPP) on growth performance, immunity, antioxidant capacity, and gut morphology of nursery pigs. In Exp. 1, 96 weaned pigs (Nebraska female × Danbred sire; 20 ± 1 d of age; initial BW = 6.06 ± 0.02 kg) were assigned to 16 pens and randomly allotted to the control (CTL; no SDPP) or the CTL + SDPP treatment in 2 phases (phase 1: d 0 to 14, 5% SDPP; phase 2: d 14 to 28, 2.5% SDPP). Blood samples were collected on d 0 and weekly thereafter to quantify IgG, IgA, and total antioxidant capacity. On d 14, pigs (n = 16; 8 pigs/treatment) were selected and euthanized for small intestine tissue and alveolar macrophage collection. On d 7, pigs fed SDPP had greater ADG, ADFI (P = 0.001), and G:F (P = 0.019) compared with CTL pigs. On d 28, pigs fed SDPP had greater BW (P = 0.024) and tended to have greater ADG (P = 0.074) and ADFI (P = 0.062) compared with CTL pigs. There were no differences between treatments for serum IgG, IgA, and total antioxidant capacity. On d 14, greater villus height (P = 0.011) and villus:crypt (P = 0.008) were observed in duodenal tissue sections obtained from SDPP-fed pigs compared with CTL pigs. To evaluate effects of SDPP on immune biomarkers, alveolar macrophages collected from 3 pigs/treatment on d 14 were cultured in vitro and challenged with lipopolysaccharide (LPS; 10 ng/mL). Therefore, 4 treatments included 1) CTL diet with no LPS, 2) CTL diet with LPS (CTL+), 3) SDPP diet with no LPS, and 4) SDPP diet with LPS. There were no diet effects on tumor necrosis factor-α gene expression or secretion by alveolar macrophages. For IL-10 gene expression, a diet × LPS interaction (P = 0.009) was observed where CTL+ had greater (P < 0.05) IL-10 mRNA abundance compared with other treatments. A second experiment was conducted to evaluate the in vitro effects of porcine plasma using model porcine jejunal epithelial cells (IPEC-J2). The treatments applied to the IPEC-J2 cells were 1) CTL, 2) CTL + 10% porcine plasma, 3) CTL + 5% porcine plasma, 4) CTL + 2.5% porcine plasma, or 5) CTL + 5% fetal bovine serum. The addition of porcine plasma increased (P < 0.001) proliferation of IPEC-J2 cells at 24 and 48 h following exposure to the respective treatments. In conclusion, dietary SDPP increased growth performance of nursery pigs during the first week postweaning and provide benefits to gut morphology possibly via increased proliferation of enterocytes.
Subject(s)
Animal Feed/analysis , Animal Nutritional Physiological Phenomena/physiology , Biomarkers/metabolism , Dietary Supplements/analysis , Gastrointestinal Tract/anatomy & histology , Plasma/chemistry , Sus scrofa/growth & development , Animals , Cell Proliferation/drug effects , Epithelial Cells/drug effects , Female , Gastrointestinal Tract/drug effects , Immunoglobulin A/blood , Immunoglobulin G/blood , Macrophages/drug effects , Sus scrofa/immunology , Sus scrofa/metabolism , Swine , Tumor Necrosis Factor-alpha/metabolism , Weight Gain/physiologyABSTRACT
An experiment consisting of two 4-wk trials was conducted to determine Lys use for growth and carcass traits in barrows and gilts fed individually or in groups. One hundred twelve growing pigs (56 barrows and 56 gilts; average initial BW of 18.6 kg) were used in each trial. Pigs were fed individually or in groups of 3. There were 28 pigs individually penned, and 84 pigs in 28 pens (3 pigs/pen). There were 2 replications per treatment in each trial for a total of 4 replications. Dietary treatments consisted of a corn-soybean meal (SBM) basal diet (0.48% Lys) and diets containing 0.56%, 0.65%, and 0.74% standardized ileal digestible (SID) Lys that were achieved by adding Lys to the basal diet from either SBM or crystalline source as L-Lys HCL (CLys). At the end of each trial, all pigs were scanned using real-time ultrasound to determine 10th-rib back fat depth and LM area, and fat-free lean gain (FFLG) was calculated. Blood samples were taken from all pigs weekly to determine plasma urea N (PUN). Pigs fed CLys and Lys from SBM were not different in final BW, ADG, ADFI, or G:F. The ADG and G:F increased linearly (P < 0.01) as dietary Lys concentration increased. The SID Lys intake increased linearly (P < 0.01) as dietary Lys concentration increased and was not different when comparing pigs fed diets with CLys or SBM. The amount of SID Lys required per unit of growth or BW gain increased linearly (about 13 to 15.50 g/kg) in pigs fed either CLys or SBM Lys. Pigs fed individually had a greater (P < 0.05) ADG (0.59 vs. 0.57 kg) and ADFI (1.36 vs. 1.29 kg) than pigs fed in groups. The SID Lys intake was greater (P < 0.05) in pigs fed individually in comparison with pigs fed in a group (8.51 vs. 8.06 g/d). Fat-free lean gain and LM area increased (P < 0.01) as dietary Lys concentration increased regardless of Lys source. Pigs fed CLys diets had a greater (P < 0.05) LM area than pigs fed SBM at 0.74% SID Lys. Gilts had a greater (P < 0.01) LM area (14.28 vs. 13.58 cm(2)) and FFLG (264 vs. 245 g/d) than barrows. Pigs fed individually with CLys had less (P < 0.01) PUN than pigs fed Lys from SBM. Barrows fed individually had less (sex × feeding method, P < 0.01) PUN than gilts (26.75 vs. 29.32 g/100 mL). The results indicate that Lys from SBM-bound and CLys source were utilized similarly for growth and carcass traits regardless of sex or feeding method.
Subject(s)
Animal Husbandry/methods , Body Composition/physiology , Lysine/pharmacology , Swine/growth & development , Animals , Female , Lysine/chemistry , Male , Swine/physiologyABSTRACT
Litter performance and progeny health status may be decreased in progeny derived from primiparous sows but improve with increasing parity. The objective was to evaluate litter performance, the production and passive transfer of Ig, and fecal microbial populations in progeny derived from first parity (P1) compared with fourth parity (P4) dams. Litter performance was recorded for P1 (n = 19) and P4 (n = 24) dams including number of pigs/litter (total born, born live, stillbirths, mummified fetuses, prewean mortality, and pigs weaned) and average litter and piglet BW at birth (d 0), d 7, d 14, and at weaning (average d 19). Blood samples were collected from all dams on d 90 and 114 of gestation and d 0 of lactation. Colostrum and milk samples were collected from each dam on d 0, 7, and 14 of lactation for quantification of IgG and IgA. Blood and fecal samples were collected from each litter (n = 6 pigs/litter) on d 1, 7, and 14 after parturition. Circulating IgG and IgA concentrations were quantified in all blood samples. Denaturing gradient gel electrophoresis (DGGE) was used to characterize similarity and diversity of fecal microbes among progeny. Progeny of P1 dams had decreased average litter BW at d 7 (25.7 vs. 30.0 kg; P < 0.03) and decreased average piglet BW throughout the experiment (d 0, 7, 14, and 19; P < 0.001) compared with P4 progeny. No parity × day interactions were observed with respect to immunoglobulin or microbial analyses. Concentrations of IgA tended to be greater (P = 0.09) in samples of colostrum and milk obtained from P4 compared with P1 dams. Serum IgG concentrations were greater (P < 0.02) in P4 progeny compared with P1 progeny. Results of DGGE revealed that P1 progeny had increased (P < 0.001) microbial similarity on d 7 and decreased (P < 0.03) microbial similarity on d 14 compared with P4 progeny. Progeny of P1 dams tended (P = 0.07) to have a greater Shannon's diversity index compared with P4 progeny on d 1, and P1 progeny had a greater (P < 0.03) Shannon's diversity index compared with P4 progeny on d 7. Litter performance, passive transfer of immunity, and progeny microbial ecology were affected by dam parity.
Subject(s)
Bacteria/isolation & purification , Immunity, Maternally-Acquired , Litter Size , Parity , Sus scrofa/physiology , Animals , Bacteria/genetics , Bacteria/metabolism , Colostrum/chemistry , Denaturing Gradient Gel Electrophoresis/veterinary , Enzyme-Linked Immunosorbent Assay/veterinary , Feces/microbiology , Female , Immunoglobulin A/blood , Immunoglobulin A/metabolism , Immunoglobulin G/blood , Immunoglobulin G/metabolism , Male , Milk/chemistry , Polymerase Chain Reaction/veterinary , Sus scrofa/immunology , Sus scrofa/microbiologyABSTRACT
Traditional selection for sow reproductive longevity is ineffective due to low heritability and late expression of the trait. Incorporation of DNA markers into selection programs is potentially a more practical approach for improving sow lifetime productivity. Using a resource population of crossbred gilts, we explored pleiotropic sources of variation that influence age at puberty and reproductive longevity. Of the traits recorded before breeding, only age at puberty significantly affected the probability that females would produce a first parity litter. The genetic variance explained by 1-Mb windows of the sow genome, compared across traits, uncovered regions that influence both age at puberty and lifetime number of parities. Allelic variants of SNPs located on SSC5 (27-28 Mb), SSC8 (36-37 Mb) and SSC12 (1.2-2 Mb) exhibited additive effects and were associated with both early expression of puberty and a greater than average number of lifetime parities. Combined analysis of these SNPs showed that an increase in the number of favorable alleles had positive impact on reproductive longevity, increasing number of parities by up to 1.36. The region located on SSC5 harbors non-synonymous alleles in the arginine vasopressin receptor 1A (AVPR1A) gene, a G-protein-coupled receptor associated with social and reproductive behaviors in voles and humans and a candidate for the observed effects. This region is characterized by high levels of linkage disequilibrium in different lines and could be exploited in marker-assisted selection programs across populations to increase sow reproductive longevity.
Subject(s)
Genetic Variation , Genome-Wide Association Study/veterinary , Receptors, Vasopressin/genetics , Reproduction/genetics , Sexual Maturation/genetics , Swine/genetics , Age Factors , Alleles , Animals , Breeding , DNA, Complementary/genetics , Female , Genetic Markers , Haplotypes , Linkage Disequilibrium , Litter Size , Parity , Phenotype , Polymorphism, Single Nucleotide , PregnancyABSTRACT
An experiment involving 854 crossbred pigs (20 replicate pens of 4 to 8 pigs per pen) was conducted at 8 experiment stations to determine the effects of acids in nursery pig diets and their inclusion amounts on growth performance using diets and weaning ages typical of those used in the United States commercial pork industry. Diets were formulated to have constant a ME and contain 1.45, 1.45, and 1.30% standardized ileal digestible Lys for phases 1, 2, and 3, respectively. The basal diets were supplemented with various types and concentrations of acid at the expense of corn (Zea mays). Treatment diets included 0% acid (control), 0.1 or 0.2% phosphoric acid, 1 or 2% organic acids, and 0.1% phosphoric acid plus 1% organic acids with or without an antibiotic. The organic acids consisted of 50% citric acid and 50% fumaric acid by weight. All but the final diet contained the antibiotic carbadox. All diets contained 3,000 mg of Zn/kg diet from zinc oxide during phases 1 and 2 and had limited acid buffering capacity, ranging from 142, 127, and 122 mEq/kg of feed for phases 1, 2, and 3, respectively. At each participating station, pigs were randomly allotted to dietary treatments on the basis of their initial BW. Sex and ancestry were equally distributed across the treatments. Results indicated that treatment effects on pig performance were observed in phases 1 and 2 but not in phase 3. In phase 1, ADG of pigs fed 0.2% phosphoric acid was greater than that of pigs fed the combination of acids with no antibiotic (P = 0.041). In phase 2, pigs fed treatments containing an antibiotic had a greater ADG than those fed the combination of acids without antibiotic (P < 0.05). Addition of acids to diets did not affect growth performance during any phase or the overall period. Over the 4-wk study, growth rate was slowest on the treatment without antibiotic, with specific differences that were often statistically significant (P < 0.05). In summary, under the conditions of this experiment, the acid treatments had no effect but the antibiotic improved growth performance.
Subject(s)
Acids/pharmacology , Animal Feed/analysis , Diet/veterinary , Swine/growth & development , Acids/chemistry , Animal Nutritional Physiological Phenomena , Animals , Buffers , Calcium/chemistry , Female , Hydrogen-Ion Concentration , Male , Phosphorus/chemistryABSTRACT
An experiment was conducted to evaluate the effects of dietary lactose alone or in combination with a yeast-dried milk product (50% dried near-dated milk and 50% dried yeast) on growth performance, fecal microbiota, and immune status in nursery pigs (Sus scrofa). A total of 108 pigs (age, 20 ± 1 d; initial BW, 6.07 ± 0.03 kg) were randomly allotted to 18 pens (6 pigs/pen; 6 pens/treatment). Dietary treatments were: 1) control, 2) control + lactose, and 3) control + lactose + 5% yeast-dried milk. Except for the control diet, diets in Phase 1 (wk 1 and 2), 2 (wk 3 and 4), and 3 (wk 5) contained 20, 15, and 5% total lactose, respectively. Blood samples were collected from all pigs at d 0, 14, 28, and 35 to determine circulating IgG, IgA, and tumor necrosis factor (TNF)-α concentrations. At d 0, 7, and 14, fecal samples were collected (n = 18; 6 pigs/treatment) to evaluate fecal microbiota using PCR-denaturing gradient gel electrophoresis. Compared with pigs fed the control diet, pigs fed lactose and lactose with yeast-dried milk had greater (P < 0.05) ADG and tended (P = 0.07) to have greater BW and ADFI during Phase 1. There were no differences for BW, ADG, or ADFI during Phase 2, 3, or the overall experimental period. A main effect of treatment was observed for circulating IgA where control pigs had greater (P < 0.01) IgA compared with pigs fed lactose with or without yeast-dried milk; however, no effects of treatment were observed (P > 0.10) for circulating IgG or TNF-α. No differences (P > 0.10) in microbial diversity indices were observed on d 7 or 14 among treatments. However, a shift in microbial composition was observed on d 7, with lactose-fed pigs having greater (P < 0.05) putative L. johnsonii staining intensity compared with control pigs and pigs fed lactose plus yeast-dried milk. On d 14, L. delbrueckii was eliminated (P < 0.04) by feeding lactose with or without yeast-dried milk. This research indicates that growth performance, immune status, and fecal microbiota are affected by dietary inclusion of lactose alone, or in combination with yeast-dried milk.
Subject(s)
Animal Feed/analysis , Lactose/pharmacology , Milk/chemistry , Swine/growth & development , Yeasts , Animals , Denaturing Gradient Gel Electrophoresis , Diet/veterinary , Feces/microbiology , Female , Gene Expression Regulation/drug effects , Gene Expression Regulation/immunology , Immunoglobulin A/blood , Immunoglobulin G/blood , Lactose/chemistry , Lactose/metabolism , Male , Milk/metabolism , Swine/blood , Swine/immunology , Swine/microbiology , Tumor Necrosis Factor-alpha/bloodABSTRACT
Two experiments were conducted to evaluate effects of corn distillers dried grains with solubles (DDGS) on growth performance and health status of weanling pigs. Experiment 1 evaluated effects of increasing concentrations of DDGS on growth performance and health of weanling pigs. Dietary treatments included 1) control (CTL), 2) 0% DDGS (0% DDGS in phase 2 and 30% DDGS in phase 3), 3) 5% DDGS (5% DDGS in phase 2 and 30% DDGS in phase 3), and 4) 30% DDGS (phases 2 and 3). Overall, pigs fed 30% DDGS during phases 2 and 3 had decreased (22.1 vs. 25.1 and 24.0 kg; P = 0.003) BW compared with CTL pigs and pigs that only received DDGS during phase 3. In addition, pigs fed 5 or 30% DDGS in phase 2 had decreased (422.7 or 390.0 vs. 468.2 g; P = 0.003) ADG compared with CTL pigs. However, pigs fed 0% DDGS during phase 2 had similar BW, ADG, and ADFI compared with CTL pigs. Experiment 2 was conducted to evaluate effects of DDGS, lactose, and their interaction on growth performance and health of weanling pigs. Dietary treatments included 1) CTL, 2) lactose (20%), 3) DDGS (15%), and 4) lactose + DDGS. Diets of interest were fed during phase 1 (d 0 to 14), and a common diet was fed during phase 2 (d 14 to 28). Pigs receiving DDGS in phase 1 had greater ADG (576.2 vs. 534.6 g; P = 0.01) and ADFI (814.9 vs. 751.6 g; P = 0.01) during phase 2 compared with non-DDGS-fed pigs. Pigs receiving lactose during phase 1 had greater ADG (214.7 vs. 177.2 g; P = 0.01) and G:F (741.0 vs. 660.3 g/kg; P = 0.01) and tended to have greater ADFI (289.3 vs. 267.6 g; P = 0.07) during phase 1 but decreased (537.7 vs. 573.1 g; P = 0.09) ADG during phase 2. Serum immunoglobulin analyses and fecal microbial profiling were conducted in both experiments as indicators of health status. No effects of dietary treatment were observed for serum immunoglobulin in either experiment. Fecal microbial profiling resulted in statistically significant effects of dietary treatment with respect to microbial similarity and diversity indices (Exp. 1) and lactic acid-producing bacteria (Exp. 2), where main effects of both lactose and DDGS were observed with respect to putative Lactobacillus reuteri (P < 0.05). Results from Exp. 1 indicate that decreased concentrations of DDGS early in the nursery phase may negatively affect growth performance; however, growth performance may be maintained when inclusion of high concentrations (30%) of DDGS is delayed until the late nursery period. Results from Exp. 2 indicate that lactose may be incorporated in nursery diets containing DDGS to help maintain growth performance, and DDGS and lactose may affect fecal microbial profiles.