[Molecular characteristic marker method of 27 breeds of common Umbelliferae Chinese herb medicinal plants by sequencing rDNA].

OBJECTIVE: To probe a molecular marker method of accrediting fingerprinting of 27 kinds of common Umbelliferae Chinese herb medicinal plants by sequencing rDNA. METHOD: The rDNA sequences of the 27 breeds of common Umbelliferae Chinese herb medicinal plants were amplified, and were digested by restriction endonuclease, and were seperated via polypropylene electrophoresis, at last 6 breeds of them were sequenced. RESULTS: The rDNA sequence fragment we gained concluded ITS1, ITS2, 5.8S complete sequence and 18S, 26S part sequence. On the electrophoresis map of PCR products digested by restriction endonuclease MSP I, 27 breeds appeared 16 kinds of characteristic map, 11 of them differ from others; and PCR products digested by restriction endonuclease HaeIII, there appeared 5 kinds of characteristic map among 27 breeds, 3 of them differ from others. The sequenced result of 6 breeds showed genes whose length extented from 652bp to 656bp were acquired. These sequences of 3 breeds which showed the same electrophoresis map after digested by restriction endonuclease HaeIII exhibited great similarity according to similar phylogenetic tree constructed on rDNA sequence. CONCLUSION: The rDNA sequence character is effective molecular marker for classifying the different Umbellerae Chinese herb medicinal plants. And the method of sequencing rDNA surpassed that of restriction fragment long polymorphism (RFLP).

ITS Sequence analysis of Chinese and Japanese medicinal plants of Angelica L / 中草药

Objective To establish the base of molecular system-theory on medicinal plants of Angelica L.and to offer molecular gists for finding the efficacy difference of them.Methods The PCR technique was utilized together with the clone technique to get the ITS gene and measure its genic sequence.Statistical analyzing and branch analyzing were carried out by Phylip software and genetic distance difference in every kinds can be calculated with MEGA3.Results ITS1-5.8 S-ITS2,belongs to one of medicinal plants of Angelica L.,its sequence length extents 600 to 629 bp.Subtracting 5.8 S,its length is 165 bp,the length of ITS is 468 bp.The medicinal plants of Angelica L.are divided into two groups by dendrogram.The genetic distance of A.sinensis from Gansu Province is obviously different from the other medicinal plants of Angelica L.,but the distance difference compared with Levisicum officinale is smaller.Conclusion It suggests that the above result be identical to the efficacy of medicinal plants of Angelica L.It may be an important reason of explaining why the efficacy between A.sinensis and other medicinal plants of Angelica L.is so different.