Whole-genome sequencing of marine water-derived Curvularia verruculosa KHW-7: a pioneering study.

Marine microorganisms are renowned for being a rich source of new secondary metabolites that are significant to humans. The fungi strain KHW-7 was isolated from the seawater collected from the Gulf of Khambhat, India, and identified as Curvularia verruculosa KHW-7. On a next-generation sequencing platform, C. verruculosa KHW-7's whole-genome sequencing (WGS) and gene annotation were carried out using several bioinformatic methods. The 31.59 MB genome size, 52.3% GC, and 158 bp mean read length were discovered using WGS. This genome also contained 9,745 protein-coding genes, including 852 secreted proteins and 2048 transmembrane proteins. The antiSMASH algorithm used to analyze genomes found 25 secondary metabolite biosynthetic gene clusters (BGCs) that are abundant in terpene, non-ribosomal peptide synthetase (NRPS), and polyketides type 1 (T1PKS). To our knowledge, this is the first whole-genome sequence report of C. verruculosa. The WGS analysis of C. verruculosa KHW-7 indicated that this marine-derived fungus could be an efficient generator of bioactive secondary metabolites and an important industrial enzyme, both of which demand further investigation and development.

Effect of earthquake sequences on risk-based catastrophe bond pricing.

Catastrophe bonds (cat bond in short) are an alternative risk-transfer instrument used to transfer peril-specific financial risk from governments, financial institutions, or (re)insurers, to the capital market. Current approaches for cat bond pricing are calibrated on seismic mainshocks, and thus do not account for potential effects induced by earthquake sequences. This simplifying assumption implies that damage arises from mainshocks only, while aftershocks yield no damage. Postearthquake field surveys reveal that this assumption is inaccurate. For example, in the 2011 Christchurch Earthquake sequence and 2016-2017 Central Italy Earthquake sequence, aftershocks were responsible for higher economic losses when compared to those caused by mainshocks. This article proposes a time-dependent aggregate loss model that takes into account seismicity clustering and damage accumulation effects in the computation of damage. The model is calibrated on the seismic events recorded during the recent 2016-2017 Central Italy Earthquake sequence. Furthermore, the effects of earthquake sequence on cat bond pricing is explored by implementing the proposed model on five Italian municipalities. The investigation showed that neglecting time-dependency may lead to higher difference (up to 45%) in the cat bond price when compared to standard approaches.

Iontophoresis-Infused Deep Topical Anesthesia and Injectable Local Anesthesia for Dental Procedures Among Pediatric Patients: Performances and Consequences.

INTRODUCTION: Exploring routes of needle-free anesthesia has drawn particular attention to the iontophoretic technique. Iontophoresis has a wide range of applications in dentistry, treating hypersensitivity, oral ulcers, non-invasive procedures of deep topical anesthesia, etc. Hence, this research was performed for a comparative assessment of topical anesthesia spray infused via iontophoresis and local anesthesia (LA) infiltration for dental procedures among 5-12-year-old patients. MATERIALS AND METHODS: A split-mouth, randomized clinical trial was undertaken over two years among study subjects aged 5 to 12 years. They were randomly assigned to one of two groups: the first (Group A - iontophoresis group) received topical anesthesia spray (Lidayn®; Pyrax Polymers, Roorkee, India) applied by iontophoresis, and the second (Group B - LA infiltration group) received local infiltration of 2% lignocaine solution (LignoTer®; Lusture Pharma, Ahmedabad, India), where primary teeth extraction or pulpectomy was performed. The Wong-Baker Facial Pain Rating Scale (WBFPRS) was used for a subjective assessment immediately following anesthesia. RESULTS: The mean value of current intensity for the extraction procedure was 9.43±0.95 mA, and the duration of application was 1.85±0.80 minutes. The mean value of current intensity for pulpectomy was 9.07±1.34 mA, and the time was 2.40±0.74 minutes. In inter-group comparison, WBFPRS scores were lower in Group A (1.96±1.64) compared to Group B (3.62±1.11), which was statistically significant with p=0.001. CONCLUSION: Compared to local infiltration, iontophoresis as a non-invasive approach for topical anesthesia was more well-received by pediatric patients.

Biogenesis and characterization of proficient silver nanoparticles employing marine procured fungi Hamigera pallida and assessment of their antioxidative, antimicrobial and anticancer potency.

OBJECTIVE: To assess the anticancer potential of biosynthesized silver nanoparticles using marine derived fungi Hamigera pallida with their antibacterial and antioxidant activities. RESULTS: The biosynthesis of silver nanoparticles (AgNPs) was assessed by the change in color from bright yellow to dark brown. UV-Visible spectroscopy revealed its stability at 429 nm; ATR-FTIR spectroscopy revealed the functional group responsible for its production; X-Ray Diffraction revealed its crystalline FCC structure resembling the peaks in the XRD pattern, corresponding to (110), (111), (200), and (311) planes; TEM imaging revealed its spherical morphology with an average particle size of 5.85 ± 0.84 nm ranging from 3.69 to 16.11 nm and Tauc's plot analysis revealed a band gap energy of 2.22 eV, revealing aptitude of AgNPs as a semiconductors. The subsequent characterization results revealed the effective synthesis of silver nanoparticles. The biosynthesized AgNPs were found to have significant antimicrobial effect against three Gram-positive and three Gram-negative bacteria. They also demonstrated higher antioxidative potential by demonstrating strong radical scavenging activity against DPPH (2, 2-diphenyl-1-picrylhydrazyl). AgNPs showed highest anticancer activity (62.69 ± 1.73%) against human breast cancer (MCF-7) cell line at 100 µg/mL with the IC50 value of 66.07 ± 2.17 µg/mL. CONCLUSIONS: This study revealed the prospect for further utilization of AgNPs by Cell free filtrate of Hamigera pallida as an antibacterial, antioxidative and anticancer agents.

Pricing risk-based catastrophe bonds for earthquakes at an urban scale.

Catastrophe risk-based bonds are used by governments, financial institutions and (re)insurers to transfer the financial risk associated to the occurrence of catastrophic events, such as earthquakes, to the capital market. In this study, we show how municipalities prone to earthquakes can use this type of insurance-linked security to protect their building stock and communities from economic losses, and ultimately increase their earthquake resilience. We consider Benevento, a middle-sized historical town in southern Italy, as a case study, although the same approach is applicable to other urban areas in seismically active regions. One of the crucial steps in pricing catastrophe bonds is the computation of aggregate losses. We compute direct economic losses for each exposed asset based on high spatial resolution hazard and exposure models. Finally, we use the simulated loss data to price two types of catastrophe bonds (zero-coupon and coupon bonds) for different thresholds and maturity times. Although the present application focuses on earthquakes, the framework can potentially be applied to other natural disasters, such as hurricanes, floods, and other extreme weather events.

Biogenic synthesis of silver nanoparticles mediated by the consortium comprising the marine fungal filtrates of Penicillium oxalicum and Fusarium hainanense along with their antimicrobial, antioxidant, larvicidal and anticancer potency.

AIM: To biosynthesize silver nanoparticles (AgNPs) using fungal isolates [DS-2 (Penicillium oxalicum) and DW-8 (Fusarium hainanense)] as well as their mixed cell-free filtrate (CFF) acting as a consortium (DSW-28) and their bio-potentials. METHODS AND RESULTS: The fungi (DS-2 and DW-8) were harvested and CFF was prepared. CFF of each fungus and their mixture were reacted with silver nitrate solution under dark conditions for the synthesis of AgNPs. The UV-Visible spectra determined the surface plasmon resonance at 438, 441 and 437 nm for the AgNPs synthesized by DS-2, DW-8 and DSW-28, respectively. The band gap energy was found between 2.21 and 2.24 eV which depicted their ability to act as a semiconductor. The TEM imaging revealed the spherical shape and small size of AgNPs. The XRD pattern exhibited the crystalline structure corresponding to their peaks. The FTIR spectra indicate the presence of different functional groups present on the surface of AgNPs. The broad-spectrum antimicrobial activity was exhibited by AgNPs. The AgNPs also act as an effective antioxidant by depicting their radical scavenging activity against DPPH. Moreover, the AgNPs also inhibited the growth of fourth instar larvae of Aedes aegypti and Culex quinquefasciatus more efficiently in a dose-dependent method. The biosynthesized AgNPs from DSW-28 showed a significant anticancer activity against MCF-7 cells. CONCLUSION: The silver nanoparticles synthesized by the CFF of two different fungi act synergistically in a consortium leading towards the production of silver nanoparticles with smaller size and higher bioactivity. SIGNIFICANCE AND IMPACT OF THE STUDY: The impressive bioactivity of the silver nanoparticles synthesized by the mixture of CFF of various fungi acting as a consortium recommends their prospective use in agriculture as well as in biomedical as an antimicrobial, antioxidant, larvicidal and anticancer agents in future.

Efficient biodegradation of Congo red dye using fungal consortium incorporated with Penicillium oxalicum and Aspergillus tubingensis.

A novel approach had been carried out to develop fungal consortium, namely, RH-2, containing two marine procured fungal isolates in order to evaluate biodegradation of recalcitrant diazo dye Congo red. The fungi were isolated from the seacoast of Diu, India. According to the ITS sequencing, the strains were identified as Penicillium oxalicum (DS-2) and Aspergillus tubingensis (DS-4). Discs of 12 mm were cut out from the edge of both the fungal isolates (DS-2 and DS-4) and inoculated in flasks consisting of potato dextrose broth with 100 mg/L Congo red for the development of fungal consortium RH-2. The degradation by the fungal consortium RH-2 was more effective than the fungal monocultures DS-2 and DS-4 with the respective degradation reaching 97.15 ± 0.15%, 68.96 ± 0.09%, and 29.96 ± 0.21% in addition of yeast extract (1% w/v) within 12 h. The influence of dextrose (1% w/v), yeast extract (1% w/v), pH 5, and salt concentration (1% w/v) enhanced the degradation potential of fungal consortium RH-2. The maximal degradation was correlated with the production of laccase (12.498 ± 0.21 U/mL) and manganese peroxidase (10.314 ± 0.25 U/mL). The catabolism of Congo red was confirmed by UV-Visible spectroscopic analysis (Congo red λ-max = 499 nm) and ATR-FTIR spectroscopic analysis. The filtrates obtained after Congo red degradation were also evaluated for microbial toxicity against bacteria (Bacillus haynesii) and phytotoxicity analysis on plant seed (Trigonella foenum) which revealed that the filtrate acquired after the treatment of Congo red by fungal consortium RH-2 was less toxic than the original dye in nature. A novel aspect is determined by the evidence of mutualistic interaction between two different fungi for the rapid decolorization and degradation of dye providing a prospective of utilizing the developed consortium RH-2 as a cost-effective approach in textile wastewater treatment for cleaner environment.

Biogenically proficient synthesis and characterization of silver nanoparticles employing marine procured fungi Aspergillus brunneoviolaceus along with their antibacterial and antioxidative potency.

OBJECTIVES: To assess the extracellular synthesis of silver nanoparticles using marine derived fungi Aspergillus brunneoviolaceus with their antibacterial and antioxidant activities. RESULTS: The biosynthesis of silver nanoparticles was estimated by the change in color from light yellow to dark brown within 36 h as the reaction progressed. UV-Visible spectroscopy exhibited its stability at 411 nm; ATR-FTIR spectroscopy depicted the functional group responsible for its production; X-Ray Diffraction denoted its crystalline FCC structure resembling the peaks in XRD pattern, corresponding to [111], [200], [220], [311] and [222] planes; TEM imaging revealed its spherical morphology with the particle size ranging from 0.72 to 15.21 nm and Tauc's plot analysis that disclosed its band gap energy as 2.44 eV that manifested the potential of AgNPs to be semiconductors. The characterization data henceforth, confirmed the efficient production of silver nanoparticles. The biosynthesized AgNPs expressed strong antibacterial activity against two Gram-positive and three Gram-negative bacteria. They also proved to possess higher antioxidative potentials by showing their potent radical scavenging activity against DPPH (2, 2-diphenyl-1-picrylhydrazyl). CONCLUSIONS: The study unfolds the prospect for further utilization of this mycogenically synthesized AgNPs as antibacterial, antioxidative and anticancer agents.

Pathway Commons 2019 Update: integration, analysis and exploration of pathway data.

Pathway Commons (https://www.pathwaycommons.org) is an integrated resource of publicly available information about biological pathways including biochemical reactions, assembly of biomolecular complexes, transport and catalysis events and physical interactions involving proteins, DNA, RNA, and small molecules (e.g. metabolites and drug compounds). Data is collected from multiple providers in standard formats, including the Biological Pathway Exchange (BioPAX) language and the Proteomics Standards Initiative Molecular Interactions format, and then integrated. Pathway Commons provides biologists with (i) tools to search this comprehensive resource, (ii) a download site offering integrated bulk sets of pathway data (e.g. tables of interactions and gene sets), (iii) reusable software libraries for working with pathway information in several programming languages (Java, R, Python and Javascript) and (iv) a web service for programmatically querying the entire dataset. Visualization of pathways is supported using the Systems Biological Graphical Notation (SBGN). Pathway Commons currently contains data from 22 databases with 4794 detailed human biochemical processes (i.e. pathways) and ∼2.3 million interactions. To enhance the usability of this large resource for end-users, we develop and maintain interactive web applications and training materials that enable pathway exploration and advanced analysis.