Effect of Soil Type: Qualitative and Quantitative Analysis of Phytochemicals in Some Browse Species Leaves Found in Savannah Biome of South Africa.

In semi-arid regions, browse plant species are used as feed and for medicinal purposes for both animals and humans. The limitation of the utilization of these species to medicinal purposes or as feed for livestock is a lack of knowledge on the concentration level of phytochemicals and other bioactive compounds found in these plants. The study sought to assay the qualitative and quantitative bioactive constituents of some browse species found in the savannah biome of South Africa, viz. Adansonia digitate, Androstachys johnsonii, Balanites maughamii, Berchemia discolor, Berchemia zeyheri, Bridelia mollis hutch, Carissa edulis, Catha edulis, Colophospermum mopane, Combretum Imberbe, Combretum molle, Combretum collinum, Dalbergia melanoxylon, Dichrostachys cinerea, Diospros lycioides, Diospyros mespiliformis, Euclea divinorum, Flueggea virosa, Grewia flava, Grewia flavescens, Grewia monticola, Grewia occidentalis, Melia azedarach, Peltophorum africanum, Prosopis velutina, Pseudolachnostylis maprouneifolia, Pterocarpus rotundifolius, Schinus molle, Schotia brachypetala, Sclerocarya birrea, Searsia lancea, Searsia leptodictya, Searsia pyroides, Senegalia caffra, Senegalia galpinii, Senegalia mellifera, Senegalia nigrescens, Senegalia polyacantha, Strychnos madagascariensis, Terminalia sericea, Trichilia emetic, Vachellia erioloba, Vachellia hebeclada, Vachellia karroo, Vachellia nilotica, Vachellia nilotica subsp. Kraussiana, Vachellia rechmanniana, Vachellia robusta, Vachellia tortilis, Vachellia tortilis subsp. raddiana, Vangueria infausta, and Ziziphus mucronata. These browse species' leaf samples were harvested from two provinces (Limpopo and North-West) of South Africa. The Limpopo province soil type was Glenrosa, Mispah, and Lithosols (GM-L), and the soil types in the North-West Province were Aeolian Kalahari Sand, Clovelly, and Hutton (AKS-CH). The harvested browse samples were air dried at room temperature for about seven days and ground for analysis. The methanol and distilled water extracts of the browse species leaves showed the presence of common phytoconstituents, including saponins, flavonoids, tannins, phenols, cardio glycosides, terpenoids, and phlobatannins, as major active compounds in browse species leaves. In the quantitative analysis, phytochemical compounds, such as soluble phenols, insoluble tannins, and condensed tannins, were quantified for common species found in both sites. Two-way ANOVA and multivariate analysis were used to test soil type and species effect on soluble phenols, insoluble tannins, and condensed tannins of woody species. Dichrostachys cinerea (0.1011% DM) in GM-L soil type and Z. mucronata (0.1009% DM) in AKS-CH soil type showed the highest (p < 0.05) concentration of soluble phenols. In AKS-CH soil type, D. cinerea (0.0453% DM) had the highest insoluble tannins concentration, while V. hebeclada had the lowest (0.0064% DM) insoluble tannins content. Vacchelia hebeclada had lower (p < 0.05) condensed tannins concentration levels than all other browse plants in both soil types. Under multivariate analysis tests, there was a significant effect (p < 0.001) of soil type, species, and soil type x species interaction on soluble phenols, insoluble tannins, and condensed tannins of woody species. In this study, most of the woody species found in GM-L soil type showed a lower amount of tannins than those harvested in AKS-CH soil type. There is a need to identify the amount of unquantified phytochemicals contained in these browse species and valorize the high-bioactive-compound browse species to enhance and maximize browsing of these browse species for animal production.

A Review on Interactions between Amino Acids and Surfactants as Well as Their Impact on Corrosion Inhibition.

Amino acid-surfactant interactions are central to numerous studies because of their increased effectiveness in chemical, biological, household and industrial use. This review will focus on the impact and effect of the physicochemical properties, temperature, pH, and surfactant chain length of the amino acid for detailed exploration of amino acids and surfactants in aqueous medium. The impact of cosolvent on self-aggregation, critical micelle concentration (CMC), and binding affinity with other biomolecules, as well as amino acid-surfactant interactions, are the epicenters. The results show that increasing the temperature causes negative enthalpy for ionic surfactants and micellization, implying that micellization and amino acids are thermodynamically spontaneous and exothermic, accompanied by positive entropy. As these physicochemical studies are additive, the amino acid and ionic surfactant interactions provide clues on protein unfolding and denaturation under different media, which further changes with a change in physiological conditions like pH, cosolvent, chain length, and temperature. On varying the pH, the net charge of the amino acid also changes and, subsequently, the binding efficiency of the amino acids to the surfactants. The presence of cosolvent causes a lowering in the hydrophobic chain, which changes the surfactant's CMC. At a reduced CMC, the hydrophobic characteristic of amino acid-surfactant associations is amplified, leading to rapid denaturation of proteins that act as propulsion under the influence of extended chain surfactants. Amino acids are one of the most intriguing classes of chemicals that produce high inhibitory efficacy. Amino acids are also a component of proteins and therefore, found in a significant part of the human body, further making them a promising candidate as corrosion inhibitors. In this review article, authors have also focused on the collection and investigation for application of amino acid-surfactant interactions in corrosion inhibition. Various predictive studies/in silico studies are also reported by many research groups, such as density functional theory (DFT) calculations and molecular dynamics simulations to obtain tentative electronic, structural, and physiochemical characteristics like energies of the highest occupied molecular orbitals and lowest unoccupied molecular orbitals, binding energy, Gibb's free energy, electronegativity, polarizability, and entropy. In silico studies are helpful for the mechanism predictions of the process occurring on metal surfaces.

Nutrients Profile of 52 Browse Species Found in Semi-Arid Areas of South Africa for Livestock Production: Effect of Harvesting Site.

The use of these browse plant species as feed supplements to livestock is restricted due to a lack of knowledge about their nutritional status. This study was conducted to evaluate the nutritive value of woody browse species found in a semi-arid, as influenced by harvesting, site (Limpopo and North West Province). Limpopo had a Glenrosa, Mispah and Lithosols (GM-L) soil type and North West sites had an Aeolian Kalahari sand, Clovelly and Hutton (AKS-CH) soil type. Fresh leaves from fifty-two trees (five trees per species) were randomly selected and harvested from the site by hand-picking. Limpopo had forty-five browse species and North West had twenty-one browse species, respectively. The samples were air dried at room temperature and ground for laboratory analysis (nutritive value). The data were subjected to one-way analysis of variance (for those species that were not common in both sites) and two-way factorial (for those species that were common in both sites) in a completely randomized design. In the GM-L soil type, M. azedarach (223.2 g/kg DM) had the highest (p < 0.05) crude protein content (CP), whereas in the AKS-CH soil type, V. hebeclada (189.2 g/kg DM) had the highest (p < 0.05) CP content. Within each species, V. nilotica. Subsp. Krasssiana had the highest (p < 0.05) dry matter digestibility (725.4 g/kg DM), non-fibrous carbohydrates (607.3 g/kg DM), digestible energy (3.375 Mcal/kg) and metabolizable energy (2.771) content when compared to all the other browse species in both GM-L and AKS-CH soils. Melia azedarach in GM-L had the highest (p < 0.05) values in most amino acids' parameters measured when compared to the same species in AKS-CH. Though the harvesting site had an effect on the nutritive value, all species, irrespective of the harvesting site, had sufficient CP to be used as a supplement to livestock exposed to the low-quality roughages. The results from this study will be useful for farmers and researchers through the provision of relevant information on how to improve livestock production. There is a need to run in vivo trials to determine the best species suitable for livestock sustainability.

Synergistic broad-spectrum antibacterial activity of Hypoxis hemerocallidea-derived silver nanoparticles and streptomycin against respiratory pathobionts.

Respiratory tract infections arise due to the introduction of microbes into the airway, disrupting the normal, healthy, complex interdependent microbiome. The selective disruption of this community can be either beneficial or dangerous. Nanoparticles are a potential tool for modifying this population. Coated silver nanoparticles (AgNPs) were synthesized using ethanolic extracts of Hypoxis hemerocallidea (EEHH), a Southern African plant used extensively in traditional medicine and the source of many bioactive secondary metabolites. The room temperature reaction between silver nitrate and EEHH forms largely spherical AgNPs with an average diameter of 6-20 nm. These nanoparticles show similar levels of antibacterial activity as the broad-spectrum antibiotic streptomycin against Bacillus cereus, Streptococcus pneumonia, Escherichia coli, Pseudomonas aeuroginosa, and Moraxella catarrhalis. However, the AgNPs synergistically increase the antibacterial activity of streptomycin when they are applied in combination (30-52%). AgNPs are reiterated to be promising dual-function antibiotics, synergistically enhancing activity while also acting as delivery agents for small molecules.

Polyethylene glycol (5,000) succinate conjugate of lopinavir and its associated toxicity using Danio rerio as a model organism.

Lopinavir (LPV), a well-known drug administered in human immunodeficiency virus (HIV) infection, has shown limitation for pediatric treatment owing to poor aqueous solubility that gives rise to limited oral bioavailability and short plasma half-life (5-6 h). Polymers such as polyethylene glycol (PEG) have been used as drug carriers to improve their solubility. This study reports the preparation of polyethylene glycol (5,000) succinate (PEG-Suc-LPV) conjugate of LPV by the esterification method. The disappearance of the 3,395 cm-1 (O-H stretch of COOH) band for Polyethylene glycol (5,000) succinate (PEG-Suc )confirms the formation ester linkage with the OH group of LPV which is also confirmed by 1H NMR analysis. The XRD for the conjugate showed a broad, amorphous peak while pure PEG, Suc, LPV are crystalline. DSC analysis showed that the conjugate exhibited new broad and diffuse peaks, confirming that they did exist in an amorphous state as multiple complexes. The conjugate showed improved solubility and activity with reduced toxicity compared to pure LPV. The solubility of LPV increased significantly from 80 to 318 ppm. Furthermore, an aquatic toxicity test using Danio rerio showed that the conjugate had a lower LC50 (60.8 ppm) when compared to the pure LPV drug LC50 (6.42 ppm). These results suggest PEG-Suc conjugate of LPV as an efficient carrier for enhanced hydrophilicity and anti-HIV property of LPV.

1-(5,7-Dihy-droxy-2,2-dimethylchroman-6-yl)ethanone.

In the title mol-ecule, C(13)H(16)O(4), the pyran ring is in a half-chair conformation. There is an intra-molecular hydrogen bond involving the ketone O atom and an H atom of a phenol group which forms an S(6) ring. The ketone O atom is also involved in an inter-molecular hydrogen bond with a different phenolic H atom of a symmetry-related mol-ecule, forming C(6) chains along the c-axis direction.