[Preliminary Study of Lonicera hypoglauca on Germination Conditions of Sand Culture Seeds and Sterilization Method of Sand Culture Seedling Sterilization].

OBJECTIVE: To explore the germination conditions of Lonicera hypoglauca sand culture seeds and the effects of sand culture seedlings sterilization. METHODS: 0.1% HgCl2 with different sterilization time, different illumination time and temperature culture condition were adopted to study the germination conditions of sand culture seeds. Different sterilization treatments and different hardening-seedling days were used to test the sterilization effect of sand culture seedlings. RESULTS: The sterilization effect of the combination of 75% ethanol 30 s + 0.1% HgCl2 5 min on Lonicera hypoglauca seeds was the optimum,with the average pollution rate of 15.56%, and the average germination rate reached 51.11%. The combination of varied temperature-room temperature under light for 12 h/d was the best, with the average germination rate peaked at 75.49%, and the average germination potential reached 68.36%. The treatment of detergent liquor scrub-tap water wash on the part above the hypocotyl, which was sand cultured under the opening condition and had no root, showed the best sterilization effect, with the average pollution rate was zero, and the average survival rate peaked at 100.00%. The sterilization effect of sand culture seedlings, which was disinfected after cleaning by detergent liquor scrub-tap water wash after hardening-seeding for 30 days, was the best, with the average pollution rate of 50.00%, and the average survival rate of 100.00%. CONCLUSION: The best sterilization effect is the combination of 75% ethanol 30 s + 0.1% HgCl2 5 min; Lighting for 12 h/d of varied temperature-room temperature is regarded as the optimum culture condition. The treatment of detergent liquor scrub-tap water wash treatment on the part above the hypocotyl,which is sand cultured under the opening condition and had no root, shows the best sterilization effect. For the sand culture seedlings, before inoculated in subculture medium, should be hardening-seedling for some days and sterilized after detergent liquor scrub-tap water wash.

[Determination of chlorogenic acid and luteoloside in lonicerae flos of different habitats and germplasms].

OBJECTIVE: To establish an UPLC-PDA method for simultaneous determination of chlorogenic acid and luteoloside in Lonicerae Flos. On the basis of developed method, the quality of Lonicerae Flos from nine habitats and two local germplasms introduced from Qufu in Shandong to Wuming in Guangxi was evaluated. METHODS: The analysis was performed on a Waters Acquity UPLC H-Class system. An Acquity UPLC BEH RP18 (100 mm x 2.1 mm,1.7 µm) column was used for all analyses. The investigated compounds were separated with a gradient mobile phase consisting of acetonitrile and 0.4% phosphoric acid solution at a flow rate of 0.2 mL/min, and the detection wavelength was set at 242 nm. RESULTS: The quality of Lonicerae Flos from Qufu was the best among Lonicerae Flos of nine habitats for its content of chlorogenic acid and luteoloside at 35.715 and 1.270 mg/g, respectively. The content of chlorogenic acid and luteoloside in Lonicerae Flos of "Jiufengyihao" and "Shuxing" introduced from Qufu to Wuming both complied with the standard of Chinese Pharmacopoeia (2010 edition). CONCLUSION: The developed UPLC-PDA method is simple, reliable and repeatable, which is helpful for the quality control of Lonicerae Flos. "Jiufengyihao" and "Shuxing" are potential germplasms for the introduction of Lonicerae Flos in Wuming.