Time-frequency representation of epicardial electrograms during ventricular fibrillation.

In the present study we quantified changes in dominant frequency, which is reciprocal of activation interval or cycle period, during ventricular fibrillation (VF). We used a Smoothed Pseudo Wigner Distribution (SPWD) to estimate time-frequency representations of epicardial electrograms recorded in swines. We used a sock with 64 electrodes spaced equally to record electrograms during 30 seconds of electrically induced VF. Results from 29 trials in three animals showed a mean dominant frequency of 6.64 Hz. We observed considerable variation in dominant frequencies during VF. Temporally, the frequencies varied by as much as +/- 1.24 Hz (2 standard deviations). Spatial variation in frequencies was +/- 1.20 Hz. Cycle periods were computed as the reciprocal of dominant frequencies obtained from the SPWD. These cycle periods were verified to be numerically similar to the cycle periods estimated using activation times detected from differentiated electrograms. Results of recent studies by others have shown that cycle periods during VF are correlated with refractory periods. Our results show that a non-stationary analysis technique such as the SPWD can be used to track spatio-temporal variation in cycle periods. These changes can be used to investigate spatio-temporal variation in cellular properties such as the effective refractory periods during VF. The substantial temporal variation in dominant frequencies that we observed suggest the possibility that the excitable gap at any epicardial location also varies considerably from one instance to another during a VF episode.

Time averaged spatial distribution of epicardial dominant frequencies during ventricular fibrillation.

Recent evidence suggests that the dominant frequencies during ventricular fibrillation (VF) may be used as indicators of dispersion in repolarization and in activation patterns. In the present study, we quantified dominant frequencies from multiple epicardial electrodes to investigate if there are differences in the averaged frequencies within the electrograms recorded from the left and the right ventricles. Further, we quantified whether the difference in average frequency between the two ventricles changed during 30 seconds of VF. Results from eighteen trials in two pigs showed that during the entire duration of VF the average dominant frequencies of all electrodes over the left ventricle were higher than those over the right ventricle (p < 0.005). The dominant frequencies are reciprocal of cycle periods or activation intervals during VF. Our results show that on average, activations in the left ventricle occurred at a faster rate than those in the right ventricle. Activation intervals at any site are determined by the refractory period at that site and the arrival time of next activation. Although differences in cellular properties may have contributed to the observed differences in activation intervals between the ventricles, it is possible that activation arrival times may be different as well. It is possible that the increased tissue mass of the left ventricle may increase the probability that any site will get excited at a faster rate after it is recovered from previous activation.