Field Evaluation of Xpert HPV Point-of-Care Test for Detection of Human Papillomavirus Infection by Use of Self-Collected Vaginal and Clinician-Collected Cervical Specimens.

The World Health Organization has recommended that testing for high-risk human papillomavirus (HPV) (hrHPV) infection be incorporated into cervical screening programs in all settings worldwide. In many high-burden, low-income countries, it will not be feasible to achieve high cervical screening coverage using hrHPV assays that require clinician-collected samples. We conducted the first evaluation of self-collected vaginal specimens compared with clinician-collected cervical specimens for the detection of hrHPV infection using the Xpert HPV test. Women aged 30 to 54 years attending two well-woman clinics in Papua New Guinea were invited to participate and provided self-collected vaginal and clinician-collected cervical cytobrush specimens. Both specimen types were tested at the point of care by using the Xpert HPV test. Women were given their cervical test result the same day. Those with a positive hrHPV test and positive examination upon visual inspection of the cervix with acetic acid were offered same-day cervical cryotherapy. A total of 1,005 women were enrolled, with 124 (12.3%; 95% confidence interval [CI], 10.3%, 14.4%) being positive for any hrHPV infection. There was a 99.4% overall percent agreement (OPA) between vaginal and cervical tests for HPV-16 (95% CI, 98.9%, 99.9%), a 98.5% OPA for HPV-18/45 (95% CI, 97.7%, 99.3%), a 94.4% OPA for other hrHPV infections (95% CI, 92.9%, 95.9%), and a 93.4% OPA for all hrHPV types combined (95% CI, 91.8%, 95.0%). Self-collected vaginal specimens had excellent agreement with clinician-collected cervical specimens for the detection of hrHPV infection using the Xpert HPV test. This approach provides for the first time an opportunity to incorporate point-of-care hrHPV testing into clinical cervical screening algorithms in high-burden, low-income settings.

Operative vaginal delivery at Port Moresby General Hospital from 1977 to 2010.

This paper presents 35 years of history of operative vaginal delivery at the Port Moresby General Hospital (PMGH). From the early 1970s when Dr. G.C. Bird was appointed as Head of Obstetrics at PMGH, vacuum extraction has been the preferred method of assisted vaginal delivery. In the early 1970s, Dr Bird began to experiment with more effective configurations of the then standard metal Malmstrom vacuum extraction cup: the Bird anterior cup was introduced in 1973 and the posterior cup in 1974. These modifications to the vacuum extractor cup allowed for more effective placement of the cup on the flexion point on the fetal head thereby facilitating more successful vacuum-assisted delivery. Between 1977 and 2010 there were a total of 11,458 vacuum extractions (average rate 3.9%) performed, with an average failure rate of 2.5%. During the same period there were 565 vaginal forceps deliveries (rate 0.2%), 11,550 caesarean sections (rate 3.9%) and 182 symphysiotomies (all for failed vacuum extraction procedures) performed. Over the period trends that are noted include a slowly rising caesarean section rate from 2% in the 1970s to nearly 5% in the current decade. Over the same period the assisted vaginal delivery rate has dropped from 10-15% in the 1970s to 3-4% since 2000. The combined fresh stillbirth and early neonatal mortality rate for infants > or = 1.5 kg and > or = 2.5 kg for the period was 11.3/1000 and 9.5/1000 respectively, and compares to a combined fresh stillbirth and early neonatal mortality rate of 8.7/1000 for assisted vaginal delivery.

Poor weight gain in late third trimester: a predictor of poor perinatal outcome for term deliveries?

In many parts of the world weighing women in antenatal clinics is no longer thought to be important. At Port Moresby General Hospital we noticed that failure to gain weight in the third trimester (or weight loss) was associated with poor perinatal outcomes. To investigate this issue we designed a prospective case-control study to determine whether poor weight gain in the third trimester is a useful clinical indicator of poor placental function by being associated with intrauterine growth restriction (IUGR) or inadequate placental function in labour by being significantly associated with suspected intrapartum fetal compromise, birth asphyxia, meconium aspiration syndrome and neonatal intensive care unit admission. We found that a failure to gain weight for more than three weeks preceding the onset of labour was significantly associated with intrapartum fetal compromise (OR 2.24), IUGR (OR 2.88), meconium aspiration syndrome (OR 4.19), the presence of thick meconium or the passage of meconium during labour (OR 2.26) and the need for admission to the neonatal intensive care unit for more than 24 hours (OR 2.22). Weighing women in the antenatal clinic setting is a useful way of screening for deteriorating or inadequate placental function, and is particularly relevant in settings where more sophisticated modalities of screening and diagnosis of placental function are not available.