Data quantifying the behaviour of macro and trace elements along the feed - manure - treated waste continuum in pig production.

Manure from animal production is commonly spread on agricultural soil as an organic fertiliser to provide macro and trace elements to crops. However, some trace elements can accumulate in the soil and become toxic to plants and microorganisms. These elements include copper (Cu) and zinc (Zn), which can be applied in large quantities when pig manure is spread. The feeding strategy and manure management (e.g. through treatment chains) are two mechanisms identified to better control the use of these elements, but their fate from the feed to the soil in pig production remains poorly documented. Better understanding the fate of Cu and Zn, as well as that of other trace and macro elements, along the feed - excreta - waste chain is required to develop alternative ways to reduce their environmental impacts. This dataset provides insight into the composition (Cu, Zn and other trace and macro elements) of organic products along two contrasting manure management chains: (1) only storage or (2) in-building separation, anaerobic digestion (AD) of solids, and digestate drying. Feed, raw slurry, liquid and solid phases after separation of the manure and AD products were sampled and then analysed to measure their total compound contents.

Porous Zinc Oxide and Plant Polyphenols as a Replacement for High-Dose Zinc Oxide on Growth Performance, Diarrhea Incidence, Intestinal Morphology and Microbial Diversity of Weaned Piglets.

The aim of this experiment is to evaluate the effects of adding porous zinc oxide, plant polyphenols, and their combination to diets without antibiotics and high-dose zinc oxide on the growth performance, diarrhea incidence, intestinal morphology, and microbial diversity of weaned piglets. A total of 150 Duroc × Landrace × Large White weaned piglets were allocated to one of five diets in a randomized complete block design with six replicates and five piglets per replicate. The experimental period was 42 d, divided into two feeding stages: pre-starter (0-14 d) and starter (14-42 d). In the pre-starter stage, the negative control group (NC) was fed a basal diet, the positive control group (PC) was fed a basal diet with 2000 mg/kg of zinc oxide, the porous zinc oxide group (PZ) was fed a basal diet with 500 mg/kg of porous zinc oxide, the plant polyphenol group (PP) was fed a basal diet with 1500 mg/kg of plant polyphenols, and the combination group (PZ + PP) was fed a basal diet with 500 mg/kg of porous zinc oxide and 1500 mg/kg of plant polyphenols. In the starter stage, the NC, PC, and PZ groups were fed a basal diet, while the PP and PZ + PP groups were fed a basal diet with 1000 mg/kg of plant polyphenols. The results showed that, (1) compared with the PZ group, adding plant polyphenols to the diet showed a trend of increasing the ADFI of weaned piglets from 14 to 28 d (p = 0.099). From days 28 to 42 and days 0 to 42, porous zinc oxide and the combination of porous zinc oxide and plant polyphenols added to the control diet improved the FCR to the level observed in pigs fed the PC diet. (2) Dietary PZ + PP tended to increase the jejunal villus height (VH) of weaned piglets (p = 0.055), and significantly increased the villus-height-to-crypt-depth ratio compared to the NC group (p < 0.05). (3) Compared with the NC group, PZ supplementation decreased the relative abundance of Firmicutes and increased the relative abundance of Bacteroidetes, and the relative abundance of Lactobacillus in the PZ and PZ + PP groups were both increased. In conclusion, porous zinc oxide and plant polyphenols may have synergistic effects in modulating intestinal health in weaned piglets and be a potential alternative to high-dose zinc oxide.

Impact of Trace Mineral Source and Phytase Supplementation on Prececal Phytate Degradation and Mineral Digestibility, Bone Mineralization, and Tissue Gene Expression in Broiler Chickens.

The objective of this study was to determine how different sources of Zn, Mn, and Cu in the feed without and with phytase affect prececal myo-inositol hexakisphosphate (InsP6) breakdown to myo-inositol (MI), prececal P digestibility, bone mineralization, and expression of mineral transporters in the jejunum of broiler chickens. A total of 896 male broiler chicks (Cobb 500) were distributed to 7 diets with 8 replicate pens (16 birds per floor pen). Experimental diets were fed from day 0 to 28. Diets were without or with phytase supplementation (0 or 750 FTU/kg) and were supplemented with three different trace mineral sources (TMS: sulfates, oxides, or chelates) containing 100 mg/kg Zn, 100 mg/kg Mn, and 125 mg/kg Cu. Prececal InsP6 disappearance and P digestibility were affected by interaction (phytase × TMS: P ≤ 0.010). In diets without phytase supplementation, prececal InsP6 disappearance and P digestibility were greater (P ≤ 0.001) in birds fed chelated minerals than in birds fed sulfates or oxides. However, no differences were observed between TMS in diets with phytase supplementation. Ileal MI concentration was increased by exogenous phytase but differed depending on TMS (phytase × TMS: P ≤ 0.050). Tibia ash concentration as well as Zn and Mn concentration in tibia ash were increased by phytase supplementation (P < 0.010), but the Cu concentration in tibia ash was not (P > 0.050). Gene expression of the assayed mineral transporters in the jejunum was not affected by diet (P > 0.050), except for Zn transporter 5 (phytase × TMS: P = 0.024). In conclusion, the tested TMS had minor effects on endogenous phytate degradation in the digestive tract of broiler chickens. However, in phytase-supplemented diets, the choice of TMS was not relevant to phytate degradation under the conditions of this study.

Effect of dietary zinc source, zinc concentration, and exogenous phytase on intestinal phytate degradation products, bone mineralization, and zinc status of broiler chickens.

This study aimed to determine the effect of Zn source and dietary level on intestinal myo-inositol hexakisphosphate (InsP6) disappearance, intestinal accumulation of lower InsP and myo-inositol (MI), prececal mineral digestibility, bone mineralization, and Zn status of broilers without and with exogenous phytase in the feed. Male Ross 308 broilers were allocated in groups of 10 to 8 treatments with 8 pens each. Experimental diets were fed from d 7 to d 28 and contained 33 mg/kg dry matter plant-intrinsic Zn. Experimental factors were phytase supplementation (0 or 750 FTU/kg) and Zn source (none [0 mg/kg Zn], Zn-sulfate [30 mg/kg Zn], Zn-oxide [30 mg/kg Zn]). Additional treatments with 90 mg/kg Zn as Zn-sulfate or Zn-oxide and phytase were included to test the effect of Zn level. No Zn source or Zn level effects were observed for ADG, feed conversion ratio, prececal P digestibility, intestinal InsP6 disappearance, and bone ash concentration. However, those measurements were increased by exogenous phytase (P < 0.001), except the feed conversion ratio, which was decreased (P < 0.001). Ileal MI concentrations were affected by phytase × Zn source interaction (P < 0.030). Birds receiving exogenous phytase and Zn supplementation had the highest MI concentrations regardless of exogenous Zn source, whereas MI concentrations were intermediate for birds receiving exogenous phytase only. Exogenous phytase and exogenous Zn source increased the Zn concentration in bone and blood of broilers (P < 0.001). In conclusion, measures of exogenous phytase efficacy were not affected by phytase × Zn source interaction. Further studies are needed to rule out an effect from Zn sources other than those tested in this study and to investigate the effect of Zn supplementation on endogenous phosphatases. The missing effect of increasing Zn supplementation from 30 to 90 mg/kg in phytase-supplemented diets gives reason to reconsider the Zn supplementation level used by the industry.

Unexpected Cu and Zn speciation patterns in the broiler feed-animal-excreta system revealed by XAS spectroscopy.

Trace minerals such as copper (Cu) and zinc (Zn) are animal nutrition supplements necessary for livestock health and breeding performance, yet they also have environmental impacts via animal excretion. Here we investigated changes in Cu and Zn speciation from the feed additive to the broiler excreta stages. The aim of this study was to assess whether different Cu and Zn feed additives induce different Cu and Zn speciation patterns, and to determine the extent to which this speciation is preserved throughout the feed-animal-excreta system. Synchrotron-based X-ray absorption spectroscopy (XAS) was used for this investigation. The principal findings were: (i) in feed, Cu and Zn speciation changed rapidly from the feed additive signature (Cu and Zn oxides or Cu and Zn sulfates) to Cu and Zn organic complexes (Cu phytate and Zn phytate). (ii) in the digestive tract, we showed that Cu and Zn phytate were major Cu and Zn species; Cu sulfide and Zn amorphous phosphate species were detected but remained minor species. (iii) in fresh excreta, Cu sulfide and Zn amorphous phosphate were major species. These results should help to: (i) enhance the design of future research studies comparing different feed additive performances; (ii) assess Cu and Zn bioavailability in the digestive tract; (iii) gain further insight into the fate of Cu and Zn in cultivated soils when poultry manure is used as fertilizer.

Interactions of zinc with phytate and phytase in the digestive tract of poultry and pigs: a review.

Phytase supplementation is gaining importance in animal nutrition because of its effect on phosphorus (P) digestibility and the increasing relevance of P for sustainable production. The potential inhibitors of phytase efficacy and phytate degradation, such as calcium (Ca) and zinc (Zn), have been a subject of intense research. This review focuses on the interactions of Zn with phytate and phytase in the digestive tract of poultry and pigs, with an emphasis on the effects of Zn supplementation on phytase efficacy and P digestibility. In vitro studies have shown the inhibitory effect of Zn on phytase efficacy. However, relevant in vivo studies are scarce and do not show consistent results for poultry and pigs. The results could be influenced by different factors, such as diet composition, amount of Zn supplement, mineral concentrations, and phytase supplementation, which limit the comparability of studies. The chosen response criteria to measure phytase efficacy, which is mainly tibia ash, could also influence the results. Compared to poultry, the literature findings are somewhat more conclusive in pigs, where pharmacological Zn doses (≥ 1000 mg kg-1 Zn) appear to reduce P digestibility. To appropriately evaluate the effects of non-pharmacological Zn doses, further studies are needed that provide comprehensive information on their experimental setup and include measurements of gastrointestinal phytate degradation to better understand the mechanisms associated with Zn and phytase supplements. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Limited impacts of high doses of dietary copper on the gut bacterial metal resistome explain negligible co-selection of antibiotic resistance.

High dietary intake of Cu has previously been linked to the selection of Cu resistance and co-selection of antibiotic resistance in specific gut bacteria. Based on a novel HT-qPCR metal resistance gene chip as combined with 16S rRNA gene amplicon sequencing and phenotypic resistance typing of Escherichia coli isolates, we here report the impacts of two contrasting Cu-based feed additives on the swine gut bacterial metal resistome and community assembly. DNA was extracted from fecal samples (n = 80) collected at day 26 and 116 of the experiment from 200 pigs allotted to five dietary treatments: negative control (NC) diet with 20 µg CuSO4 g-1 and four diets added 125 or 250 µg CuSO4 g-1 feed or 125 or 250 µg Cu2O g-1 feed to the NC diet. Dietary Cu supplementation reduced the relative abundance of Lactobacillus, but it had negligible impacts on bacterial community composition relative to the gut microbiome maturation effect (time). The relative importance of different bacterial community assembly processes was not markedly affected by the dietary Cu treatments, and differences in swine gut metal resistome composition could be explained primarily by differences in bacterial community composition rather than by dietary Cu treatments. High dietary Cu intake (250 µg Cu g-1) selected for phenotypic Cu resistance in E. coli isolates, but surprisingly it did not result in increased prevalence of the Cu resistance genes targeted by the HT-qPCR chip. In conclusion, the lacking impacts of dietary Cu on the gut bacterial metal resistome explain results from a previous study showing that even high therapeutic doses of dietary Cu did not cause co-selection of antibiotic resistance genes and mobile genetic elements known to harbor these genes.

Impacts of dietary copper on the swine gut microbiome and antibiotic resistome.

Restrictions on antibiotic growth promoters have prompted livestock producers to use alternative growth promoters, and dietary copper (Cu) supplementation is currently being widely used in pig production. However, elevated doses of dietary Cu constitute a risk for co-selection of antibiotic resistance and the risk may depend on the type of Cu-based feed additives being used. We here report the first controlled experiment investigating the impact of two contrasting Cu-based feed additives on the overall swine gut microbiome and antibiotic resistome. DNA was extracted from fecal samples (n = 96) collected at four time points during 116 days from 120 pigs allotted to three dietary treatments: control, divalent copper sulfate (CuSO4; 250 µg Cu g-1 feed), and monovalent copper oxide (Cu2O; 250 µg Cu g-1 feed). Bacterial community composition, antibiotic resistance genes (ARGs), and mobile genetic elements (MGEs) were assessed, and bioavailable Cu ([Cu]bio) was determined using whole-cell bacterial bioreporters. Cu supplementation to feed increased total Cu concentrations ([Cu]total) and [Cu]bio in feces 8-10 fold and at least 670-1000 fold, respectively, but with no significant differences between the two Cu sources. The swine gut microbiome harbored highly abundant and diverse ARGs and MGEs irrespective of the treatments throughout the experiment. Microbiomes differed significantly between pig growth stages and tended to converge over time, but only minor changes in the bacterial community composition and resistome could be linked to Cu supplementation. A significant correlation between bacterial community composition (i.e., bacterial taxa present) and ARG prevalence patterns were observed by Procrustes analysis. Overall, results of the experiment did not provide evidence for Cu-induced co-selection of ARGs or MGEs even at a Cu concentration level exceeding the maximal permitted level for pig diets in the EU (25 to 150 µg Cu g-1 feed depending on pig age).

How copper can impact pig growth: comparing the effect of copper sulfate and monovalent copper oxide on oxidative status, inflammation, gene abundance, and microbial modulation as potential mechanisms of action.

The beneficial effect of elevated concentrations of copper (Cu) on growth performance of pigs has been already demonstrated; however, their mechanism of action is not fully discovered. The objective of the present experiment was to investigate the effects of including Cu from copper sulfate (CuSO4) or monovalent copper oxide (Cu2O) in the diet of growing pigs on oxidative stress, inflammation, gene abundance, and microbial modulation. We used 120 pigs with initial body weight (BW) of 11.5 ± 0.98 kg in 2 blocks of 60 pigs, 3 dietary treatments, 5 pigs per pen, and 4 replicate pens per treatment within each block for a total of 8 pens per treatment. Dietary treatments included the negative control (NC) diet containing 20 mg Cu/kg and 2 diets in which 250 mg Cu/kg from CuSO4 or Cu2O was added to the NC. On day 28, serum samples were collected from one pig per pen and this pig was then euthanized to obtain liver samples for the analysis of oxidative stress markers (Cu/Zn superoxide dismutase, glutathione peroxidase, and malondialdehyde, MDA). Serum samples were analyzed for cytokines. Jejunum tissue and colon content were collected and used for transcriptomic analyses and microbial characterization, respectively. Results indicated that there were greater (P < 0.05) MDA levels in the liver of pigs fed the diet with 250 mg/kg CuSO4 than in pigs fed the other diets. The serum concentration of tumor necrosis factor-alpha was greater (P < 0.05) in pigs fed diets containing CuSO4 compared with pigs fed the NC diet or the diet with 250 mg Cu/kg from Cu2O. Pigs fed diets containing CuSO4 or Cu2O had a greater (P < 0.05) abundance of genes related to the intestinal barrier function and nutrient transport, but a lower (P < 0.05) abundance of pro-inflammatory genes compared with pigs fed the NC diet. Supplementing diets with CuSO4 or Cu2O also increased (P < 0.05) the abundance of Lachnospiraceae and Peptostreptococcaceae families and reduced (P < 0.05) the abundance of the Rikenellaceae family, Campylobacter, and Streptococcus genera in the colon of pigs. In conclusion, adding 250 mg/kg of Cu from CuSO4 or Cu2O regulates genes abundance in charge of the immune system and growth, and promotes changes in the intestinal microbiota; however, Cu2O induces less systemic oxidation and inflammation compared with CuSO4.

Copper is a nonrenewable mineral resource that is essential for all biological organisms. After banning the antibiotics, copper has received considerable attention due to its antimicrobial properties that improve performance in animals when fed over the minimum requirement. The present study evaluated two sources of Cu (copper sulfate and monovalent copper oxide) compared with a nonsupplemented diet and the likely mechanism of action which leads to improved pig performance. Pigs fed high concentrations of copper sulfate showed increased liver oxidation and inflammatory indicators in the blood. Elevated concentrations of Cu improved intestinal epithelial barrier function, modulation of inflammatory responses, increased beneficial microbes, and reduced pathogens in the gut. Therefore, supplementation of high levels of Cu appears to be effective in promoting pig growth, but therapeutic doses of Cu sulfate increase the inflammatory response.

Environmental Impacts and Their Association With Performance and Excretion Traits in Growing Pigs.

The selection of pigs for improved production traits has been, for a long time, the major driver of pig breeding. More recently, because of the increasing concern with the environment, new selection criteria have been explored, such as nitrogen (N) excretion. However, many studies indicate that life cycle assessment (LCA) provides much better indicators of environmental impacts than excretion. Therefore, the objective of this study was to investigate, using a modeling approach, the relationships between production traits and LCA impacts of individual growing pigs calculated at the farm gate for 1 kg of body weight gain. Performances of pigs were simulated for 2-phase (2P) and precision feeding (PR), using the InraPorc population model (on 1,000 pigs). Nitrogen excretion was positively correlated with feed conversion ratio (FCR; r = +0.96), climate change (CC; r = +0.96), acidification potential (AC; r = +0.97), eutrophication potential (EU; r = +0.97), and land occupation (LO; r = +0.96), whatever the feeding program. However, FCR appeared to be a better indicator of LCA impacts, with very high and positive correlations (r > +0.99) with CC, AC, EU, and LO for both feeding programs. The CC, AC, and EU impacts of pig production for PR feeding were 1.3, 10, and 7.5% lower than for 2P, respectively, but the correlations within each outcome were very similar among feeding programs. It was concluded that the use of FCR as a selection criterion in pig breeding seems to be a promising approach to associate improved performance and low environmental impact of pig fattening.

Recent Advances in Understanding the Influence of Zinc, Copper, and Manganese on the Gastrointestinal Environment of Pigs and Poultry.

Zinc, copper, and manganese are prominent essential trace (or micro) minerals, being required in small, but adequate, amounts by pigs and poultry for normal biological functioning. Feed is a source of trace minerals for pigs and poultry but variable bioavailability in typical feed ingredients means that supplementation with low-cost oxides and sulphates has become common practice. Such trace mineral supplementation often provides significant 'safety margins', while copper and zinc have been supplemented at supra-nutritional (or pharmacological) levels to improve health and/or growth performance. Regulatory mechanisms ensure that much of this oversupply is excreted by the host into the environment, which can be toxic to plants and microorganisms or promote antimicrobial resistance in microbes, and thus supplying trace minerals more precisely to pigs and poultry is necessary. The gastrointestinal tract is thus central to the maintenance of trace mineral homeostasis and the provision of supra-nutritional or pharmacological levels is associated with modification of the gut environment, such as the microbiome. This review, therefore, considers recent advances in understanding the influence of zinc, copper, and manganese on the gastrointestinal environment of pigs and poultry, including more novel, alternative sources seeking to maintain supra-nutritional benefits with minimal environmental impact.

Inclusion of dicopper oxide instead of copper sulfate in diets for growing-finishing pigs results in greater final body weight and bone mineralization, but reduced accumulation of copper in the liver.

An experiment was conducted to test the hypothesis that inclusion of Cu oxide (Cu2O) in diets for growing-finishing pigs improves body weight (BW) and bone mineralization, and reduces accumulation of Cu in the liver compared with pigs fed diets containing Cu sulfate (CuSO4). Two hundred growing pigs (initial BW: 11.5 ± 0.98 kg) were allotted to a randomized complete block design with 2 blocks of 100 pigs, 5 dietary treatments, 5 pigs per pen, and a total of 8 pens per treatment. Treatments included the negative control (NC) diet that contained 20 mg Cu/kg, and 4 diets in which 125 or 250 mg Cu/kg from CuSO4 or Cu2O were added to the NC diet. The experiment was divided into 4 phases and concluded when pigs reached market weight. Pig weights were recorded on day 1 and at the end of each phase and feed provisions were recorded throughout the experiment. On the last day of phases 1 and 4, 1 pig per pen was sacrificed to obtain samples of liver and spleen tissue, and the right metacarpal was collected. Results indicated that pigs fed diets containing 250 mg Cu/kg from CuSO4 had greater BW at the end of phases 1 and 2 than pigs fed NC diets. Pigs fed diets containing 250 mg Cu/kg from Cu2O had greater (P < 0.05) BW at the end of phases 1, 2, 3, and 4 compared with pigs fed NC diets, and these pigs also had greater BW at the end of phases 3 and 4 than pigs fed all other diets. Pigs fed the diets with 250 mg Cu/kg tended to have greater (P < 0.10) feed intake than pigs fed the NC diet at the end of phase 2, and for the overall experimental period, pigs fed diets containing 250 mg Cu/kg from Cu2O had greater (P < 0.05) feed intake than pigs on all other treatments. However, no differences in gain:feed ratio were observed among treatments. Copper accumulation in liver and spleen increased with Cu dose, but at the end of phase 1, pigs fed 250 mg Cu/kg from CuSO4 had greater (P < 0.05) Cu concentration in liver and spleen than pigs fed 250 mg Cu/kg from Cu2O. Pigs fed diets containing 250 mg Cu/kg from Cu2O had greater (P < 0.05) quantities of bone ash and greater (P < 0.05) concentrations of Ca, P, and Cu in bone ash than pigs fed NC diets or the 2 diets containing CuSO4, but Zn concentration in bone ash was less (P < 0.05) in pigs fed diets containing 250 mg Cu/kg from Cu2O. To conclude, supplementing diets for growing pigs with Cu2O improves growth performance and bone mineralization with less Cu accumulation in liver compared with pigs fed diets containing CuSO4.

Pesquisa de enteroparasitas em morangos frescos comercializados in natura em Goiânia, Goiás / Research on enteroparasites in fresh strawberries commercialized in natura in Goiânia, Goiás

Objetivo: O presente estudo objetivou pesquisar enteroparasitas em morangos frescos comercializados in natura no município de Goiânia, Goiás. Métodos: Foram analisadas 51 amostras de morangos, sendo 25 oriundas de supermercados e 26 de rua/comércio livre, entre agosto/2019 e dezembro/2019, em diversos bairros das regiões norte, noroeste, sul e central do município de Goiânia, Goiás, empregando-se as técnicas de Faust, Hoffman, Ritchie e Coloração de Kinyoun (Ziehl-Neelsen modificado). Resultados: Das 51 amostras analisadas, 78,4% foram positivas para enteroparasitas. Das quarenta amostras positivas, em 25 (62,5%) foram identificados apenas cistos de protozoários, em oito (20,0%) apenas ovos de nematódeos e em sete (17,5%) cistos de protozoários e ovos de nematódeos, simultaneamente. As espécies encontradas foram: Ascaris lumbricoides, Entamoeba coli, Entamoeba histolytica, Endolimax nana e Giardia lamblia. Todos os enteroparasitas foram identificados pelas técnicas de Hoffman e Faust. Conclusão: O estudo evidenciou elevada contaminação dos morangos comercializados em Goiânia, Goiás por enteroparasitas, com destaque para os protozoários. O consumo de morangos frescos in natura pode oferecer risco à saúde de seus consumidores, sendo crucial orientar a população sobre a correta higienização deste alimento antes de seu consumo a fim de evitar a ocorrência de enteroparasitoses.

Objective: This study aimed to research enteroparasites in fresh in natura strawberries marketed in the city of Goiânia, Goiás. Methods: A total of 51 strawberry samples were analyzed. These samples, 25 were from supermarkets and 26 from street/free trade, between August/2019 and December/2019, in several neighborhoods in the north, northwest, south and central regions of the municipality of Goiânia, Goiás, using the techniques of Faust, Hoffman, Ritchie and Kinyoun Coloring (modified Ziehl-Neelsen). Results: Of the 51 samples analyzed, 78.4% were positive for enteroparasites. Of the 40 positive samples, in 25 (62.5%) just protozoan cysts were identified, in eight (20.0%) just nematode eggs and in seven (17.5%) protozoan cysts and nematode eggs, simultaneously. The species found were: Ascaris lumbricoides, Entamoeba coli, Entamoeba histolytica, Endolimax nana, and Giardia lamblia. The enteroparasites founded were identified by the techniques of Hoffman and Faust.Conclusion: The study showed high contamination of strawberries marketed in Goiânia, Goiás by enteroparasites, with emphasis on protozoa. The consumption of fresh strawberries, in natura, can pose a risk to the health of its consumers, being crucial to guide the population on the correct hygiene of this food before its consumption to avoid the occurrence of enteroparasitosis.

Burnout inventory for referees (bir): psychometric properties for brazilian soccer referees / Inventário de burnout para árbitros (bir): propriedades psicométricas para árbitros brasileiros de futebol

ABSTRACT The objective of the study is to analyze the psychometric measurements of the Burnout Inventory for Referees (BIR) offering validity evidence of the instrument through the analysis of its internal structure, estimating reliability indicators and comparing the total score of Burnout in referees and assistant referees. Participants were 284 soccer referees from six Brazilian states. The mean age was 31.3 years old (±6.1), being 255 male participants (89.7%), and the average experience time of the referees was 6.9 years (±5.5 years). The BIR consists of a questionnaire of 9 items, arranged on a 7 points likert scale. The result of the exploratory factor analysis pointed to one factor that explained 53.1% of the data variance. The Comparative Fit Index = 0.923 (95% CI 0.883-0.950) and Non-Normed Fit Index = 0.897 (95% CI 0.844-0.933) indicates suitability to a single-factor. There was no burnout difference between referees and assistant referees. The instrument presented a reliability index (McDonald´s omega) of 0.823. It is concluded that the factor structure of BIR´s version for Brazilian referees focuses on a single dimension that guided the development of the instrument. This result is also different from that found in other studies carried out previously using the instrument.

RESUMO O objetivo do estudo é analisar as propriedades psicométricas do Inventário de Burnout para Árbitros (BIR) oferecendo evidências de validade do instrumento por meio da análise de sua estrutura interna, estimando indicadores de confiabilidade e comparar o escore total de Burnout em árbitros centrais e árbitros assistentes. Participaram 284 árbitros de futebol de seis Estados brasileiros. A idade média foi 31,3 anos(±6,1), sendo 255 do sexo masculino (89,7 %) e tempo de experiência na arbitragem médio 6,9 anos (±5,5 anos). O BIR consiste em um questionário de 9 itens, dispostos em uma escala likert de 7 pontos. O resultado da análise fatorial exploratória apontou para a presença de um fator que explicou 53,1% da variância dos dados. O ajuste do modelo Comparative Fit Index = 0,923 (IC de 95% 0,883-0,950) e Non-Normed Fit Index = 0,897 (IC de 95% 0,844- 0,933), indicam adequação do modelo de um fator único. Não houve diferença de burnout entre árbitros assistentes e centrais. O instrumento apresentou índice de confiabilidade ômega de McDonald = 0,823. Conclui-se que o BIR apresenta estrutura fatorial centrada na dimensão exaustão física e emocional, diferente do seu modelo inicialmente proposto, e de outros estudos realizados anteriormente com o instrumento.

Educação em saúde sexual e reprodutiva na adolescência / Sexual and reproductive health education in adolescence

Objetivo: Relatar a experiência de discentes de enfermagem em oficinas com foco na saúde sexual e reprodutiva de adolescentes. Metodologia: Trata-se de um estudo descritivo, do tipo relato de experiência, realizado em ambiente escolar com a participação de adolescentes entre 15 e 17 anos. As atividades desenvolvidas consistiram em oficinas sobre Mudanças Biopsicossociais, Sexualidade, Violência Sexual, Gravidez na Adolescência e Infecções Sexualmente Transmissíveis. Para o desenvolvimento das oficinas, foram considerados os preceitos éticos da Resolução n° 466/2012 não havendo variáveis que possibilitem a identificação dos participantes da oficina. Resultados: Verificou-se a participação assídua do público alvo com diversos questionamentos e a aplicabilidade positiva das oficinas na prevenção e promoção da saúde. Destacou-se o papel do enfermeiro como principal mediador em promover educação em saúde nas escolas e nas comunidades. Conclusão: A implementação de atividades de educação sexual e reprodutiva na adolescência mostrou-se relevante para a redução de vulnerabilidades, esclarecimentos de dúvidas e conscientização dessa população.

Objective: To report the experience of nursing students in workshops focusing on the sexual and reproductive health of adolescents. Methodology: This is a descriptive, experience-type study conducted in a school environment with the participation of adolescents between 15 and 17 years of age. The activities developed consisted of workshops about Biopsychosocial Changes, Sexuality, Sexual Violence, Teen Pregnancy and Sexually Transmitted Infections. For the development of the workshops, the ethical precepts of Resolution No. 466/2012 were considered and there were no variables that allowed the identification of workshop participants. Results: It was verified the assiduous participation of the target public with several questions and the positive applicability of the workshops in the prevention and promotion of health. The role of the nurse as the main mediator in promoting health education in schools and in communities was highlighted. Conclusion: The implementation of sexual and reproductive education activities in adolescence was relevant for the reduction of vulnerabilities, clarification of doubts and awareness of this population.

Effects of dietary lycopene on the protection against oxidation of muscle and hepatic tissue in finishing pigs.

OBJECTIVE: The objective of this study was to evaluate the effect of different levels of lycopene supplementation on the carcass traits, meat quality, concentration of lipid oxidation products and antioxidant potential in the meat and liver of finishing barrows and gilts. METHODS: A total of 40 barrows and 40 gilts were allotted in a completely randomized block design, arranged in a 2×5 factorial scheme, consisting of two sexes (barrows and gilts) and five dietary levels of lycopene (0, 12.5, 25.0, 37.5, and 50.0 mg/kg). In addition, four storage times (0, 24, 48, and 72 h), at 4°C, were added to the model to evaluate the longissimus lumborum muscle. RESULTS: An interaction (p = 0.010) was observed between storage periods and dietary lycopene levels. The unfolding of the interaction (lycopene×period) showed a decreasing concentration of malondialdehyde concentration as the dietary lycopene increased, at all storage periods. No interactions (p>0.050) were observed for the 2,2 diphenyl 1 picrylhydrazyl (DPPH) in the pork. However, the percentage of DPPH radical inhibition reduced (p = 0.001) up to 72 h. Additionally, there was a linear increase (p = 0.001) in the capture of DPPH radicals by antioxidants, as the dietary lycopene increased. No interactions were observed (p>0.05) between the evaluated factors in liver. However, lipid oxidation was reduced by supplementing lycopene in pig diets. The capture of the DPPH radical, resulted increase in the antioxidant power exerted by lycopene in the liver (p = 0.001). The concentrations of the thiobarbituric acid reactive substances and DPPH in the liver were affected by sex (p = 0.001). CONCLUSION: Dietary supplementation of lycopene reduced the water loss during thawing and was effective in protecting against oxidation of the longissimus lumborum muscle and liver until 72 hours of storage, and the best results were obtained by supplementing with 50.0 mg of lycopene/kg of diet.

Prática de enfermeiros obstetras na assistência ao parto humanizado em maternidade de alto risco / Practice of obstetric nurses in humanized childbirth care in a high-risk maternity

RESUMO Objetivo compreender a prática de enfermeiros obstetras na assistência ao parto de gestantes de alto risco. Métodos estudo qualitativo, com sete enfermeiros obstetras que atendem a gestantes de alto risco. Dados coletados por meio de entrevistas semiestruturadas, os quais foram submetidos à análise de conteúdo para tratamento dos dados. Resultados evidenciou-se que os enfermeiros conheciam, executavam e estimulavam as boas práticas durante a assistência, mas reconheciam fatores que impediam o desenvolvimento dessas práticas de forma satisfatória. Relataram sobre as motivações por essas práticas e as insatisfações encontradas no percurso da função. Conclusão os enfermeiros participantes eram motivados e demonstraram interesse pelas práticas humanizadas, mas eram insatisfeitos com as diversas dificuldades encontradas, como profissionais desatualizados e estrutura organizacional do serviço.

ABSTRACT Objective to understand the practice of obstetric nurses in childbirth care for high-risk pregnant women. Methods a qualitative study, with seven obstetric nurses that attend high-risk pregnant women. The data collection took place using semi-structured interviews that were submitted to content analysis for data treatment. Results it has been evident that nurses knew, performed, and encouraged good practices during care, but identified factors that prevented the development of these practices in an acceptable way. They reported on the reasons for these practices and the dissatisfaction met in the course of their work. Conclusion the enrolled nurses were motivated and showed interests in humanized practices, but were unpleased with the distinct challenges met, such as outdated professionals and the service's organizational structure.

Crescimento e desenvolvimento infantil em crianças hospitalizadas: atuação do enfermeiro / Child growth and development in hospitalized children: nurse's performance

Objetivo: descrever a experiência sobre a atuação da enfermagem frente aos marcos de desenvolvimento infantil e a orientação aos familiares para o conhecimento e acompanhamento eficaz do desenvolvimento. Metodologia: estudo descritivo de abordagem qualitativa, do tipo relato de experiência, realizado em um Hospital de Referência de uma capital do Nordeste no período de Abril de 2018 durante a disciplina de Saúde da Criança e do Adolescente. Resultados: É essencial a elaboração de ações para o conhecimento e acompanhamento dos pais e cuidadores ao desenvolvimento das crianças para a promoção de um ambiente seguro e suscetível ao estímulo do potencial de cada criança. Conclusão: O enfermeiro é responsável por realizar e orientar os pais e cuidadores a importância do acompanhamento e desenvolvimento infantil. O acompanhamento é primordial para a detecção precoce de atrasos motores, cognitivos ou psicossociais, sendo possível perceber sinais e sintomas com a correta orientação, através do cartão de vacina no qual a criança recebe após o nascimento, e quando bem orientados os pais evitam atrasos ou um diagnostico tardio.

Objective: to describe the experience about nursing performance in relation to child developmental milestones and guidance to family members for knowledge and effective monitoring of development. Methodology: this is a descriptive study of a qualitative approach, type of experience report, carried out in a Reference Hospital of a capital of the Northeast in the period of April 2018 during the discipline of Child and Adolescent Health. Results: it is essential to develop actions for the knowledge and monitoring of parents and caregivers to the development of children to promote a safe environment and susceptible to stimulate the potential of each child. Conclusion: nurses are responsible for conducting and guiding parents and caregivers on the importance of child follow - up and development. Follow-up is essential for the early detection of motor, cognitive or psychosocial delays, and it is possible to perceive signs and symptoms with the correct orientation through the vaccine card that the child receives after birth, and when well-oriented parents avoid delays or a late diagnosis.

Optimization and validation of an SBSE-HPLC-FD method using laboratory-made stir bars for fluoxetine determination in human plasma.

Depression is the largest cause of disability worldwide, affecting 350 million people. Notwithstanding that clinical trials demonstrate antidepressants efficacy, the efficient response can vary individually concerning therapeutic dosage. Although important, plasma levels monitoring remains an analytical challenge whereas clean-up and pre-concentration represent critical steps. Therefore, this study aims to develop, optimize and validate a method for fluoxetine determination in human plasma, employing a laboratory-made device consisting of a PDMS stir bar sorptive for extraction, coupled with high-performance liquid chromatography-fluorescence detection (SBSE-HPLC-FD). Optimization involved sorption-desorption steps. For sorption, temperature and time were assessed by factorial and central composite design approaches, taking into account the desirability and the response surface results, with stirring speed also examined. For desorption kinetics and ultrasonic and magnetic stirring mode were evaluated. The proposed method after validation was robust, linear (25.00-1000.00 ng mL-1 , R2 > 0.98) and presented good intra- (RSD 4.18%) and inter-day-assay (RSD 11.60%) precision and accuracy (recovery 109.60%), allowing reliable quantitation without interference. The method was successfully applied to real samples. SBSE-HPLC-FD could represent a feasible alternative with good cost-benefit for low-volume samples and therapeutic drug monitoring, as well as contributing to correlation studies between plasma fluoxetine levels and clinical response, which is still little studied.

Estresse e Coping de Árbitros de Futebol no Teste Físico FIFA / Stress and Coping of Soccer Referees in the FIFA Physical Test / Estrés y Coping de Árbitros de Fútbol en la Prueba Física FIFA

Árbitros de futebol parecem sofrer o impacto do estresse em sua atuação, especialmente em situações definidoras de seu sucesso profissional, como a Avaliação Física FIFA, que determina a aptidão física para atuação em campeonatos estaduais e nacionais. Estratégias de coping determinam a percepção de situações estressoras, e consequentemente, a forma de enfrentamento das mesmas. Este estudo teve por objetivo avaliar o impacto do teste físico FIFA nos níveis de estresse e coping de árbitros de futebol atuantes em campeonatos estaduais e nacionais (Federação Mineira de Futebol ­ FMF e Confederação Brasileira de Futebol ­ CBF, respectivamente). Responderam a Escala de Estresse Percebido, que avalia estresse negativo (distress) e coping, 42 árbitros da FMF e 17 da CBF, antes e após o teste físico FIFA. Na avaliação pré-teste, verificou-se que os árbitros da CBF apresentaram histórico de mais testes físicos, treinos por semana, anos de experiência, além de escores mais altos em coping (p<0,05). Quando controladas as diferenças iniciais, árbitros CBF e FMF não diferiram significativamente em coping e distress no pós-teste físico. Diante da confirmação parcial das hipóteses, sugere-se a condução de novas pesquisas na área que incluam a avaliação do tipo de estratégia de coping utilizado e características de personalidade dos árbitros....(AU)

Soccer referees seem to feel the impact of stress in their performance, especially in situations that define their success at soccer, such as the FIFA Fitness Test, which determines the physical aptitude of the professional to perform in state and national tournaments. Coping strategies determine the perception of stressful situations and the consequent way of facing them. This study aimed to evaluate the impact of the FIFA Fitness Test on the stress and coping levels of soccer referees, which perform at the state and at the national level (FMF ­ Soccer Federation of Minas Gerais; and CBF ­ Brazilian Soccer Federation, respectively). 42 referees of FMF and 17 of CBF answered to The Perceived Stress Scale, which assess distress and coping, before and after the FIFA Test. At the pre-test, the CBF referees showed a major number of physical tests, weekly trainings and years of experience, and higher coping (p < 0,05). Controlling for initial differences, CBF and FMF referees were not significantly different on coping and distress after the test. Given the partial confirmation of the hypothesis, it is suggested to conduct further research in the area that includes the evaluation of the type of coping strategy used and personality characteristics....(AU)

Árbitros de fútbol parecen sufrir el impacto del estrés en su rendimiento, especialmente en la definición de las situaciones de su éxito profesional, como la prueba de Evaluación Física FIFA, que determina la aptitud física para trabajar en los campeonatos estatales y nacionales. Las estrategias de coping determinan la percepción de las situaciones de estrés, y por lo tanto, la forma de enfrentarlos. Este estudio tuvo como objetivo evaluar el impacto del teste físico FIFA en los niveles de estrés y coping en árbitros de fútbol activos en campeonatos estatales y nacionales (Federación Minera de Fútbol ­ FMF, y la Confederación Brasileña de Fútbol ­ CBF, respectivamente). 42 árbitros FMF y 17 CBF respondieron a la Escala de Estrés Percibido, que evalúa el estrés negativo (distress) y coping, antes y después de la prueba FIFA. En la evaluación previa a la prueba, se encontró que los árbitros CBF tenían una historia de más pruebas físicas, entrenamientos por semana, años de experiencia, y las puntuaciones más altas de coping (p < 0,05). Cuando se controlan las diferencias iniciales, CBF y FMF árbitros no difirieron significativamente en el coping y distress después de la prueba física. Dada la confirmación parcial de las hipótesis iniciales, se sugiere llevar a cabo nuevas investigaciones incluyendo la evaluación del tipo de estrategia de coping utilizado y de la personalidad de los árbitros....(AU)