ABSTRACT
The article deals with the results of using cultivated allofibroblasts in topical treatment of burn wounds. Allofibroblasts obtained from the derma of burnt individuals were transplanted to the burn wounds of 28 patients. In 12 among 15 patients (80%) with IIIa-IIIb degree burns transplantation induced epithelialization of the burns in 12.1 +/- 2.4 days. Thirteen patients had deep burns, only in 3 of them with a circumscribed burn area transplantation proved effective. Complications of transplantation were encountered in 5 patients--rejection of the transplant in 2 and its suppuration in 3 patients. Allofibroblast transplantation may be applied as an independent method of treatment in IIIa, b degree burns.
Subject(s)
Burns/surgery , Skin Transplantation/methods , Adolescent , Adult , Burns/pathology , Cells, Cultured/transplantation , Fibroblasts/transplantation , Graft Rejection/pathology , Humans , Male , Middle Aged , Skin/cytology , Skin Transplantation/adverse effects , Skin Transplantation/pathology , Time Factors , Transplantation, HomologousABSTRACT
The authors discuss the first results of the use of cultivated human allofibroblasts in the treatment of persisting wounds of the donor areas in 13 patients with deep burns and cicatricial deformities caused by the burns. Transplantation of cell cultures led to complete epithelialization of the wounds in 6-10 days. Complication, suppuration of the grafted material, was recorded in one case. Reaction of graft rejection was not encountered. The authors believe that the effect of the transplantation is due to stimulation of the epithelialization processes by the cultivated fibroblasts.
Subject(s)
Burns/surgery , Cicatrix/surgery , Fibroblasts/transplantation , Skin Transplantation , Burns/complications , Cells, Cultured , Chronic Disease , Cicatrix/etiology , Cicatrix/physiopathology , Humans , Reoperation , Treatment Outcome , Wound HealingABSTRACT
The work discusses the results of experience of long-year's standing of the staff of the Vishnevsky Institute of Surgery in the creation of a biological bandage with the use of cultivated human skin cells. A graft of cultivated human allofibroblasts was used for closure of burn wounds for the first time. Its efficacy was studied in 86 cases of burns. The use of cultivated allofibroblasts is indicated in bordering III ab degree burns and sluggishly healing donor areas formed after autodermoplasty. Study of the efficacy of cultivated allofibroblasts in the treatment of deep burns in combination with dermoplasty with a 1:6 perforated skin autograft are continued. The low cost and technical simplicity of the method allow cultivated fibroblast grafts to be recommended for wide clinical practice.
Subject(s)
Biological Dressings , Burns/surgery , Skin Transplantation/methods , Burns/physiopathology , Cells, Cultured , Fibroblasts/transplantation , Humans , Severity of Illness Index , Skin/cytology , Skin/injuries , Wound HealingABSTRACT
An autoplasty method for treatment of burns was worked out by the authors. In lab conditions a monolayer of epithelium is grown out from a piece of owner skin. Its size is 10 times (and more) as big as the initial material. The article deals with the results of treatment of 12 patients who had the transplantation of monolayer epithelium. Indications and contraindications are enclosed. The article concludes by the appreciation of data which were obtained during this work.
Subject(s)
Burns/surgery , Adolescent , Adult , Child , Culture Techniques , Epithelium/transplantation , Humans , Middle Aged , Skin Transplantation/methods , Time Factors , Transplantation, AutologousABSTRACT
An allograft from man cultured fibroblasts has been received. Results of its transplantation on long not-healing donor wounds in 13 patients after autodermatoplastic operations have been studied. Expressed stimulating influence of cultured fibroblasts on donor wound epithelialization processes has been stated. In spite of allograft applications there has been no tearing away reactions. It has been connected with elimination of superficial cell antigens during cultivation. Microbe contamination of more than 10(2) is the contraindication for grafting.
Subject(s)
Fibroblasts/transplantation , Skin Transplantation , Wound Healing , Wounds and Injuries/surgery , Cells, Cultured , Humans , Transplantation, HomologousABSTRACT
This paper deals with the possibility of closing of burn surfaces of skin wounds with cultivation of patients' fibroblasts. Transplantation of cultured fibroblasts was performed in 11 patients with 30-75% of a body surface burned. The results obtained indicate a prospect of this method of burn treatment.
Subject(s)
Burns/therapy , Fibroblasts/transplantation , Occlusive Dressings , Wound Healing/physiology , Body Surface Area , Burns/pathology , Burns/physiopathology , Cells, Cultured , Humans , Time FactorsABSTRACT
Experimental animal and human biopsy material has been evaluated for the possibility of culturing skin epidermocytes. Insulin and hydrocortisone were used as biostimulants of adequate cell proliferation. Morphological studies revealed that the culture obtained exists as a monolayer of epidermocytes free of other skin elements.