Recording maximal intercuspation and border positions of the mandible with intraoral scanner using the acquisition software's multi-occlusion function.

PURPOSE: This in vitro study was conducted to investigate the accuracy of intraoral scanner (IOS) for recording maximal intercuspal position (MIP) and border positions of the mandible. MATERIALS AND METHODS: Maxillary and mandibular master casts were articulated in MIP, protrusive, and lateral interocclusal position sequentially on a semi-adjustable articulator. For each articulation relation, sites of occlusal contacts (SOCs) and sites of clearance (SCs) were identified on the master casts with articulating paper (reference sites). IOS was used to take full arch scans and nine virtual interocclusal records (VIRs) for virtual articulation of models. Virtual SOCs and SCs were detected with 3D processing software and compared to those identified with the articulating paper. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were calculated for each articulation relation. RESULTS: For MIP, IOS showed adequate sensitivity and NPV of 100%, and specificity and a PPV of 99%. For protrusive position, the IOS showed a sensitivity and a NPV of 100%, a high PPV of 86%, and a specificity of 83%. For lateral positions, the specificity and the PPV were high (93% and 79%, respectively), but the sensitivity and the NPV were below the clinically acceptable limits (28% and 56%, respectively). CONCLUSION: IOS displayed clinically acceptable accuracy for recording MIP and protrusive border mandibular position. However, IOS had less accuracy for lateral border mandibular position.

Surface roughness, wear, and abrasiveness of printed and milled occlusal veneers after thermomechanical aging.

STATEMENT OF PROBLEM: Occlusal veneers are a conservative method of reestablishing vertical dimensions of occlusion (VDO) for worn teeth. A restorative material used for occlusal veneers should have a smooth surface, adequate wear resistance, and low abrasiveness to the antagonists, as total wear of occlusal veneers and their antagonists determines the stability of the reestablished VDO. Studies on roughness, wear, and abrasiveness of occlusal veneers are scarce. PURPOSE: The purpose of this in vitro study was to assess the surface roughness, wear, and abrasiveness of printed and milled occlusal veneers after thermomechanical aging against natural cusps and restorative materials. MATERIAL AND METHODS: Forty-eight extracted mandibular first molars were prepared for occlusal veneers and scanned with an intraoral scanner. The scans were exported to a computer-aided design program to design the occlusal veneers in 3 groups (n=16) according to the restorative material: group IP: milled lithium disilicate; group EN: milled hybrid ceramic (Vita Enamic), group VA: printed hybrid ceramic (Varseosmile Crown plus). The occlusal veneers in each group were bonded to their corresponding abutments and subjected to 250 000 mechanical cycles and a simultaneous 1000 thermal cycles in a mastication simulator. During thermomechanical aging, half of the specimens of each study group (n=8) were opposed by natural cusps (antagonist C) and the other half by antagonist cusps fabricated from the same restorative material as the occlusal veneers (antagonist R). The wear of the occlusal veneers and their antagonists was assessed with a 3-dimensional processing software program. The surface roughness of the veneers was assessed with a contact profilometer. The results were statistically analyzed with a 2-way ANOVA followed by the post hoc Tukey HSD test (α=.05). RESULTS: The 2-way ANOVA indicated a significant effect for the material and the antagonist on surface roughness, wear, and abrasiveness (P<.05). When opposed by antagonist C, VA showed significantly higher surface roughness than IP and EN (P<.001). VA had significantly higher surface wear followed by EN and IP (P<.001). IP caused significantly higher wear to antagonist C than EN and VA (P<.001). In addition, IP had significantly higher total wear (combined wear of veneers and their antagonists) followed by VA and EN (P<.001). When opposed by antagonist R, no significant difference was found among the 3 materials for surface roughness (P=.08), material wear (P=.12), opposing wear (P=.11), or total wear (P=.11). CONCLUSIONS: Both material and antagonist had a significant effect on surface roughness and occlusal stability when occlusal veneers were fabricated to restore VDO. VA had significantly more surface roughness and wear than EN and IP when opposed by natural cusps. IP abraded natural cusps significantly more than EN and VA. The 3 tested materials showed similar roughness, wear, and abrasiveness when opposed by the same material.

Evaluation of fracture resistance and marginal fit of implant-supported interim crowns fabricated by conventional, additive and subtractive methods.

BACKGROUND: Interim crowns are utilized for restoring implants during and after the process of osseointegration. However, studies on adaptation and fracture strength of implant-supported interim crowns are rare. AIM OF THE STUDY: The aim of this in vitro study is evaluating marginal fit and fracture resistance of conventional, subtractive, and additive methods of fabricating implant-supported interim crowns. MATERIALS AND METHODS: An implant was placed in an epoxy resin model with a missing first molar. A scan body was attached, and scanned with an intraoral scanner (IOS), the STL file was used to fabricate eighteen master models with standardized implant digital analogue spaces. The digital analogues and their corresponding abutments were attached to the master models and scanned with the IOS, the STL files were used to fabricate eighteen crowns using three different techniques (n = 6): conventional (CR); from Autopolymerizing composite resin, subtractive (SM); milled from PMMA resin blanks, and additive (AM); from 3D printed resin material. The crowns were fitted and cemented on their corresponding abutments and subjected to cyclic loading and thermocycling. The marginal fit was evaluated using a stereomicroscope. The crowns were then loaded until fractured in a universal testing machine. The Shapiro-Wilk and the Kolmogorov-Smirnov tests revealed that data of Marginal gap was non-parametric. Kruskal-Wallis test followed by the Dunn test was used (α = 0.05). While data of Fracture resistance test was parametric. ANOVA (F-test) was used followed by the Tukey test (α = 0.05). RESULTS: For marginal gap, a significant difference was shown between the study groups (P = .001) according to Kruskal-Wallis test. Groups SM and AM had significantly lower marginal gap values compared to group CR (P = .003). No significant difference was found between groups SM and AM (P = .994). For fracture resistance, One-way ANOVA revealed a significant difference in fracture resistance between study groups (P < .001). Group SM had significantly higher fracture strength followed by group AM and group CR (P = .001). CONCLUSIONS: Group SM and AM showed better marginal adaptation than group CR. Group SM showed superior fracture resistance compared to other groups. All study groups showed acceptable marginal gap and fracture resistance.

Accuracy of virtual articulation in maximal intercuspal position in direct and indirect digital workflows for fixed partial denture fabrication.

PURPOSE: To assess the accuracy of virtual articulation in direct digital workflow (DDW) and indirect digital workflow (IDW) in arches prepared for fixed partial dentures (FPDs). MATERIALS AND METHODS: Five pairs of master models were used in this study representing different clinical scenarios of full dentate (FD), and prepared arches for fixed partial dentures as follows: FD group, short span posterior (SSP group), long span posterior (LSP group), short span anterior (SSA group), and long span anterior (LSA). Fourteen pairs of interarch reference points were added to each set of master models to measure linear interarch distance with a caliper (reference measurements). The direct digital workflow included digital scans and virtual articulation with buccal scan images using an intraoral scanner. The indirect digital workflow included conventional polyvinylsiloxane impressions and bites followed by pouring, mounting, and scanning the stone models in a laboratory scanner. The scanned stone models were virtually articulated with buccal scanning in the laboratory scanner. Digital linear interarch measurements on all virtually-articulated models were compared with reference measurements. The absolute mean differences in linear interarch distances were calculated. The Mann-Whitney test was used for statistical analysis (α = .05). RESULTS: The direct digital workflow produced significantly less linear interarch deviations in the virtually articulated models compared to the indirect digital workflow for all study groups (P < .05). However, the direct digital workflow had significantly less accuracy for virtual articulation in long span posterior, long span anterior, and short span anterior groups compared to the full dentate group.  CONCLUSIONS: Both workflows produced virtually-articulated models with acceptable accuracy. However, the direct digital workflow had significantly better accuracy in all assessed clinical scenarios.

Effect of cement spacer on fit accuracy and fracture strength of 3-unit and 4-unit zirconia frameworks.

BACKGROUND: Cement spacer is essential for compensating deformation of zirconia restoration after sintering shrinkage, allowing proper seating and better fracture resistance of the restoration. Studies assessing the effect of cement spacer on fit accuracy and fracture strength of zirconia frameworks are missing in the literature. Therefore, the aim of this study was to evaluate the effect of different cement spacer settings on fit accuracy and fracture strength of 3-unit and 4-unit zirconia frameworks. METHODS: Sixty standardized stainless-steel master dies were manufactured with 2 prepared abutments for fabricating 3-unit and 4-unit zirconia frameworks. The frameworks were assigned into 6 groups (n = 10) according to cement spacer setting (30 µm, 50 µm, and 80 µm) as follows: 3-unit frameworks; 3u-30, 3u-50, 3u-80, and 4-unit frameworks; 4u-30, 4u-50, and 4u-80. The frameworks were assessed for fit accuracy with the replica method. The specimens were cemented to their corresponding dies, and the fracture strength was measured in a universal testing machine. The Weibull parameters were calculated for the study groups and fractured specimens were inspected for failure mode. Two-Way ANOVA followed by Tukey test for pairwise comparison between study groups (α = 0.05). RESULTS: The cement spacer had a significant effect on both fit accuracy and fracture strength for 3-unit and 4-unit frameworks. The 50 µm spacer had significantly better fit accuracy followed by 80 µm, and 30 µm spacers. Both 50 µm and 80 µm spacers had similar fracture strength, and both had significantly better strength than 30 µm spacer. CONCLUSIONS: For both 3-unit and 4-unit zirconia frameworks, 50 µm cement spacer can be recommended over 30 µm and 80 µm spacers for significantly better fit accuracy and adequate fracture strength.

Effect of laser etching on surface characteristics and porcelain bond to soft milled and direct metal laser sintered cobalt chromium alloys.

STATEMENT OF PROBLEM: The surface topography of metal substrate can affect its bond to porcelain. A neodynium-doped yttrium aluminum garnet (Nd:YAG) laser has been introduced to modify the metal surface topography and improve porcelain bond strength. However, studies on the effect of laser etching on metal to porcelain bond strength are lacking. PURPOSE: The purpose of this in vitro study was to determine the effect of Nd:YAG laser etching on the surface roughness and wettability of and the porcelain bond strength to cobalt chromium (Co-Cr) substrate fabricated by milling and direct metal laser sintering (DMLS). MATERIAL AND METHODS: Thirty-two 0.5×3×25-mm Co-Cr specimens were fabricated by milling soft Co-Cr (M group) and DMLS Co-Cr metal powder (DML group). The surface topography of representative specimens from each study group was assessed under a scanning electron microscope (SEM) and an atomic force microscope (AFM). All specimens were assessed for surface roughness using a contact profilometer, and for wettability with a contact angle goniometer. Half of the specimens of each study group (n=8) were subjected to surface laser etching by using a Nd:YAG laser. The specimens subjected to etching were assessed again for surface topography and wettability. All specimens in both study groups were veneered with porcelain. The porcelain bond strength was tested with a 3-point bend test in a universal testing machine. The results were statistically analyzed with 2-way ANOVA test followed by the post hoc Tukey test for pairwise comparisons (α=.05). RESULTS: After etching, the M group had a higher mean ±standard deviation Ra and Rz of 2.9 ±0.6 and 17.7 ±3.2 µm and significantly better wettability and bond strength of 79 ±6 and 52 ±13 MPa. In contrast, after etching, the DMLS group had a significantly lower Ra and Rz of 7.9 ±2.4 and 41.8 ±9.3 µm and significantly lower wettability and bond strength of 87 ±4 and 70 ±10 MPa. The DMLS group had a significantly higher roughness and bond strength than the M group before and after laser etching. The SEM and AFM showed different surface topography in the study groups. CONCLUSIONS: The manufacturing process of Co-Cr substrate had a significant effect on surface characteristics and porcelain bond strength. Laser etching improved the surface topography and bond strength of milled Co-Cr but not of DMLS Co-Cr.

Effect of modifying occlusal cement spacer on the fit accuracy of digitally manufactured zirconia crowns.

STATEMENT OF PROBLEM: Cement spacer has a crucial influence on the adaptation of fixed restorations. Recently, digitally fabricated zirconia crowns have become more popular, but studies on the effect of occlusal cement spacer on the fit accuracy of digitally designed and milled zirconia crowns are lacking. PURPOSE: The purpose of this in vitro study was to investigate the effect of modifying digital occlusal spacer on the marginal and internal fit of digitally manufactured zirconia crowns. MATERIAL AND METHODS: A maxillary molar typodont tooth was prepared for a zirconia crown, scanned with the Medit i700 intraoral scanner (IOS), and the standard tessellation language (STL) file was used to produce 3-dimensionally (3D) printed definitive dies assigned to 3 groups (n=12). All dies were scanned with the IOS, and the obtained STL files were exported to a computer-aided design (CAD) software program for the designing and milling of 36 complete contour zirconia crowns. The zirconia crown design was identical in the 3 groups for all parameters (default parameters in the CAD software program) with a 80-µm radial spacer 1 mm from the finish lines. The occlusal cement spacer was adjusted to 80 µm, 40 µm, and 0 µm for group 80-80, group 40-80, and group 0-80 respectively. The internal and marginal fit of the crowns were measured on their corresponding definitive dies with the replica technique. The Kruskal-Wallis test followed by the Dunn test with the Bonferroni correction was used for statistical analysis of the results (α=.05). RESULTS: The modification of occlusal cement spacer significantly affected the marginal and internal fit of digitally manufactured crowns (P<.05). Group 0-80 and group 40-80 had similar marginal gap values, which were significantly lower than those of group 80-80 (P<.017). For internal fit accuracy, group 0-80 displayed significantly lower gap values than group 40-80 and group 80-80 for all measured areas. Group 40-80 had significantly lower gap values than group 80-80 at the mid-occlusal and axio-occlusal areas (P<.017). CONCLUSIONS: Modifying occlusal cement spacer significantly affected the fit of digitally fabricated zirconia crowns. Reducing or eliminating occlusal spacer resulted in significantly improved fit accuracy.

Color difference for shade determination with visual and instrumental methods: a systematic review and meta-analysis.

BACKGROUND: Shade determination is a critical step for the fabrication of a satisfactory restoration. Visual shade selection with conventional shade guides is subjective and influenced by variables related to light, observer, and object. Shade selection devices have been introduced to provide subjective and quantitative shade values. This systematic review and meta-analysis aimed to compare the color difference for shade selection with visual and instrumental methods. METHODS: An initial search was conducted on databases (MEDLINE via PubMed, Scopus, and Web of Science) in addition to a manual search through references of identified articles. Studies comparing the accuracy of visual and instrumental shade selection based on ΔΕ were included in data synthesis. Mean differences (MDs) and 95% confidence intervals (CIs) were calculated to estimate the effect size for global and subgroup meta-analysis using the inverse variance weighted method and random-effects model (P ˂ 0.05). Results were presented as forest plots. RESULTS: The authors identified 1776 articles from the initial search. Seven in vivo studies were included in the qualitative analysis of which six studies were included in the meta-analysis. For the global meta-analysis, the pooled mean (95% CI) was - 1.10 (- 1.92, - 0.27). Test for overall effect showed that instrumental methods were significantly more accurate than visual methods with significantly less ΔΕ (P = 0.009). Test for subgroup difference showed that the type of instrumental shade selection method used had a significant effect on accuracy (P ˂ 0.001). Instrumental methods including spectrophotometer, digital camera, and smartphone showed significantly better accuracy compared with visual shade selection (P ˂ 0.05). The greatest mean difference was found between the smartphone and visual method with a mean (95% CI) of - 2.98 (- 3.37, - 2.59) with P ˂ 0.001 followed by digital camera and spectrophotometer. There was no significant difference in accuracy between IOS and visual shade selection (P = 1.00). CONCLUSIONS: Instrumental shade selection with a spectrophotometer, digital camera, and smartphone showed significantly better shade matching compared with a conventional shade guide, whereas IOS did not improve the shade matching significantly compared with shade guides. REVIEW REGISTRATION: PROSPERO CRD42022356545.

Fit of zirconia fixed partial dentures fabricated from conventional impressions and digital scans: A systematic review and meta-analysis.

STATEMENT OF PROBLEM: Available studies comparing fit accuracy of zirconia fixed partial dentures (FPDs) fabricated from conventional impressions and digital scans provide contradictory results. In addition, studies have been heterogeneous and of a limited number to provide conclusive evidence. PURPOSE: The purpose of this systematic review and meta-analysis was to compare the marginal and intaglio fit of tooth-supported zirconia FPDs fabricated from conventional impressions and digital scans and to investigate the effect of different variables on the fit results. MATERIAL AND METHODS: An electronic search was performed on the National Library of Medicine (NLM), Cochrane Central Register of Controlled Trials, and Scopus databases. In addition, a manual search was carried out. Studies comparing the fit of tooth-supported zirconia FPDs fabricated from conventional impressions and digital scans and reporting sufficient data for qualitative and quantitative analysis were included. Standard mean differences (SMDs) and 95% confidence intervals (CI) were calculated for meta-analysis. Subgroup analysis was performed to study the effect of variables including restoration form (monolithic or framework), units number, intraoral scanner (IOS) type, conventional impression material, spacer thickness, and abutments region. RESULTS: The initial search resulted in a total of 608 articles. Nine articles were included in the analysis (1 clinical and 8 in vitro) evaluating 118 restorations. Digital scan displayed significantly better marginal fit (P<.001; SMD: -0.68; 95% CI: -0.92, -0.09) and intaglio fit (P=.020; SMD: -0.51; 95% CI: -0.94, -0.42). Test for subgroup difference showed a significant influence of only impression material type (P=.008) and units number (P=.030) on marginal fit. Digital scan showed significantly better marginal accuracy for 3-unit FPDs than 4-unit FPDs (P<.001; SMD: -1.02; 95% CI: -1.41, -0.63). In addition, digital scanning had significantly better marginal fit with polyvinyl siloxane than polyether (P<.001; SMD: -0.98; 95% CI: -1.32, -0.64). A cement spacer ≤50 µm improved both marginal and intaglio fit in the digital group. The TRIOS scanner resulted in the best performance in the digital group for marginal fit. CONCLUSIONS: Digital scanning provides significantly better marginal and intaglio fit than conventional impression making for fabricating zirconia FPDs up to 4 units, either in monolithic form or frameworks and at any region of the arch. However, further clinical studies are recommended to obtain more substantial results.

In vivo precision of digital static interocclusal registration for full arch and quadrant arch scans: a randomized controlled clinical trial.

BACKGROUND: Clinical studies comparing the accuracy of digital and conventional records for static interocclusal registration are lacking. Therefore, the purpose of this clinical study was to assess the precision of digital interocclusal registration compared to conventional registration for full arch and quadrant arch conditions. METHODS: Nine individuals with complete natural dentition were enrolled in this study. Each participant received digital scans, conventional impressions, and static interocclusal records according to the following groups: group DF, full arch digital scans and bilateral buccal scans with Medit i700 intraoral scanner (IOS); group DQ, quadrant arch digital scans and unilateral buccal scans with Medit i700 IOS; group CF, full arch polyvinyl siloxane (PVS) impressions and PVS interocclusal records; group CQ, quadrant arch PVS impressions and PVS interocclusal records. For group CF and group CQ, the impressions were poured, mounted with the silicone bites, scanned with a laboratory scanner, and articulated virtually with buccal scans with the laboratory scanner. In each group, each participant received three interocclusal records to repeat the virtual articulation three times and the articulated models were saved as STL files. The STL files were imported into a 3D-processing software to calculate the discrepancies between repeated measures using best-fit-alignment method. The significance between the study groups was calculated with two-tailed paired t-test at P < 0.05. RESULTS: For full arch, group DF showed significantly better precision with a mean value of 31 ± 19 µm compared to 204 ± 81 µm for group CF (P = 0.0003). Similarly, for quadrant arch, group DQ showed significantly better precision with a mean value of 18 ± 6 µm compared to 255 ± 136 µm for group CQ (P = 0.0009). No significant difference in precision was found between quadrant arch and full arch when the digital or the conventional method was used. CONCLUSIONS: The digital approach had significantly better precision for static interocclusal registration compared to the conventional method in both full and quadrant arch situations. Trial Registry This clinical trial was registered on 06/07/2022 in the Pan African Clinical Trial Registry database, the number for the registry is PACTR202207648490275.

Accuracy of intraoral scanners for static virtual articulation: A systematic review and meta-analysis of multiple outcomes.

STATEMENT OF PROBLEM: Static virtual articulation with intraoral scanners (IOSs) can eliminate the human errors related to conventional articulation methods and enhance accuracy. A systematic review and meta-analysis based on multiple accuracy outcomes can combine the available literature and provide an evidence-based conclusion. PURPOSE: The purpose of this systematic review and meta-analysis was to investigate whether static virtual articulation with IOSs has acceptable accuracy for completely dentate and partially edentulous patients. MATERIAL AND METHODS: An electronic search was conducted on the PubMed, Web of Science, and Scopus databases. In addition, a manual search through reference lists of selected articles was performed. Clinical and in vitro studies evaluating the accuracy of static virtual articulation with IOSs based on diagnostic accuracy, trueness, precision, and occlusion of fabricated fixed restorations were included. The pooled results included sensitivity, specificity, positive likelihood ratio (+LR), negative likelihood ratio (-LR), diagnostic odds ratios (DORs), mean values, and 95% confidence intervals (CIs). Results were presented as forest plots. The summary receiver operator characteristic (SROC) was displayed to summarize the diagnostic test performance. RESULTS: The initial search resulted in a total of 5061 articles, of which 29 articles were included in the analysis. The virtual interocclusal records (VIRs) with IOSs showed acceptable pooled results for diagnostic accuracy (sensitivity: 0.76; specificity: 0.80; DOR: 14.77, area under the SROC (AUC): 0.87; cut off point Q∗: 80; +LR: 3.66; -LR: 0.31). The pooled data for trueness and precision were within the acceptable limits. The pooled mean (95% CI) for trueness based on linear deviations was 243.53 (144.90, 342.17). The pooled mean (95% CI) for precision based on 3D deviation of articulated models was 54.97 (43.49, 66.46). In addition, the included studies reported accurate occlusion for fixed restorations fabricated by using VIRs with IOSs. Moreover, most of the studies on trueness based on virtual occlusal contact area reported acceptable accuracy. CONCLUSIONS: VIRs with IOSs had acceptable accuracy for static virtual articulation.

Fit of monolithic multilayer zirconia fixed partial dentures fabricated by conventional versus digital impression: a clinical and laboratory investigations.

OBJECTIVES: This study aimed to compare the accuracy of conventional and digital impressions based on the fit of produced three-unit fixed partial dentures (FPDs) in vivo and the trueness and precision of both impression techniques. MATERIALS AND METHODS: Twelve patients received a conventional polyether impression (group C, control, n=12) and a digital impression with CS3500 (group D, test, n=12) for each participant. Monolithic multilayer zirconia FPDs were fabricated, and the internal and marginal fit were assessed using the replica technique. Trueness and precision of both impression methods were assessed in vitro. A master model was used to create a reference scan. The master model received conventional impressions (group C, control, n=5) and digital impressions (group D, test, n=5). The virtual models of both groups were superimposed over the reference scan (5 superimpositions) using a three-dimensional (3D) processing software, and the 3D deviations were measured and averaged to obtain trueness value. For precision, the virtual models of each group were superimposed over each other (10 superimpositions) and the average deviation value was calculated. The data were analyzed using one-tailed Mann-Whitney U test at P ≤ 0.05. RESULTS: Group D resulted in a significantly better marginal and internal fit (30.91±15.15 and 30.86±13.57 µm for group D and 40.02±19.50 and 41.86±18.94 µm for group C). The mean values of trueness and precision for conventional and digital techniques were comparable (trueness: 62.8±5.45 and 62.72±12.01 µm and precision: 56.47±27 and 60.9±14.5 µm, respectively). CONCLUSIONS: No significant difference was found between conventional and digital impressions in 3D datasets accuracy. In addition, both techniques resulted in FPDs with an acceptable clinical fit. However, the FPDs fabricated using the digital technique displayed better internal and marginal fit. CLINICAL RELEVANCE: The applied impression technique as well as the computer-aided processing of the produced virtual models can significantly affect the fit of the final restoration. Direct digital impression is recommended over conventional impression for fabricating accurate monolithic zirconia 3-unit FPDs. TRIAL REGISTRATION: This clinical trial was retrospectively registered on August 11, 2020, in the Pan African Clinical Trial Registry database, and the number for the registry is PACTR202008685699453.

Thermal inactivation kinetics of peroxidase and polyphenol oxidase from pomegranate arils (Punica granatum L. cv. Wonderful).

Thermal inactivation of peroxidase (POD) and polyphenol oxidase (PPO) in both pomegranate arils crude enzyme extract and fresh juice was investigated. The optimum conditions for reactions were studied using a mixture of guaiacol and H2 O2 as substrate for POD and pyrogallol for PPO. The experimental work indicated that optimum pH for both enzymes was 7.0; meanwhile optimum temperature was 30°C for POD and 25°C for PPO. Michaelis-Menten constant (Km) values for POD were 8.33 and 8.00 mM for guaiacol and H2 O2, respectively. The Km value was 5.88 mM pyrogallol for PPO. Thermal inactivation results revealed that the inactivation kinetics followed a monophasic first-order model. Activation energies (Ea) were 74.68 and 112.97 kJ/mol for POD and PPO, respectively. Therefore, POD was more heat-stable than PPO. This result is very useful to optimize pomegranate processing (canning or freezing), which represents the most important food industries in many tropical and subtropical regions. PRACTICAL APPLICATIONS: Pomegranate has been attracted more interest due to its antioxidant and nutritional values. For industrial applications, there is a growing interest to obtain pomegranate products such as juice, jams, food supplements, etc. It is well established that the residual activities of endogenous enzymes in either raw materials or processed products may cause loss of quality during storage. Inhibition of enzymatic browning is an important factor to maintain pomegranate quality. This work offers a better insight of characterization of browning enzymes and their thermosatbility, which will be useful to control the possible browning of pomegranate arils during processing and storage. In addition, the results of this study is very important in pomegranate processing where it showed a higher temperature with shortened time can be applied.

Enrichment of rice-based extrudates with Cactus Opuntia dillenii seed powder: a novel source of fiber and antioxidants.

The present study investigated the effects of adding the powder of cactus Opuntia dillenii (O. dillenii) seeds on the functional properties, fiber, antioxidants and acceptability of rice-based extrudates. The control blend consisting basically of rice flour was replaced with O. dillenii seed powder at 2, 4, 6, 8, 10, 15 and 20% then extruded at the optimum processing conditions. The extruded products were evaluated for their chemical composition, functional properties, color attributes, antioxidant activity and sensory characteristics. The results revealed that adding O. dillenii seeds powder enhanced the fiber, phenolics, flavonoid contents and antioxidant activity of extrudates. Expansion, bulk density and breaking strength were significantly decreased, while water absorption index, water solubility index and oil absorption index were significantly increased compared to the control. Furthermore, the mean scores of sensory evaluation indicated clear improvements in all tested sensory attributes, which significantly increased by increasing the level of O. dillenii seed powder up to 15%. The results confirmed that O. dillenii seed powder could be incorporated in rice to develop snack products of acceptable functional, nutritional and sensory properties.

Robust linear and non-linear models of NIR spectroscopy for detection and quantification of adulterants in fresh and frozen-thawed minced beef.

This study aimed to evaluate the potential of near infrared spectroscopy (NIRS) as a fast and non-destructive tool for detecting and quantifying different adulterants in fresh and frozen-thawed minced beef. Partial least squares regression (PLSR) models were built under cross validation and tested with different independent data sets, yielding determination coefficients (R(P)(2)) of 0.96, 0.94 and 0.95 with standard error of prediction (SEP) of 5.39, 5.12 and 2.08% (w/w) for minced beef adulterated by pork, fat trimming and offal, respectively. The performance of the developed models declined when the samples were in a frozen-thawed condition, yielding R(P)(2) of 0.93, 0.82 and 0.95 with simultaneous augments in the SEP of 7.11, 9.10 and 2.38% (w/w), respectively. Linear discriminant analysis (LDA), partial least squares-discriminant analysis (PLS-DA) and non-linear regression models (logistic, probit and exponential regression) were developed at the most relevant wavelengths to discriminate between the pure (unadulterated) and adulterated minced beef. The classification accuracy resulting from both types of models was quite high, especially the LDA, PLS-DA and exponential regression models which yielded 100% accuracy. The current study demonstrated that the VIS-NIR spectroscopy can be utilized securely to detect and quantify the amount of adulterants added to the minced beef with acceptable precision and accuracy.