Parts, Period, and Extraction Solvents as Parameters Influencing Harungana madagascariensis Lam. ex Poir. (Hypericaceae) Antibacterial Activity.

The study of the conditions which affect the pharmacological activity of medicinal plants is the basis for the development of phytomedicine. This study aimed at identifying some optimal conditions for antibacterial activity of extracts of Harungana madagascariensis. The leaves, bark, and roots were harvested in Douala, Littoral Region of Cameroon, in August 2019 at three times of the day: early in the morning (6 a.m.), at midday (12 p.m.), and in the afternoon (6 p.m.). They were dried at room temperature and ground to give a fine powder. Each powder was macerated in methanol for 72 h and boiled in distilled water and palm wine. The obtained extracts were evaluated for their antibacterial activity by broth microdilution method on 31 clinical and 4 reference bacterial strains. The results show that the best extraction yield was obtained using the bark extracted from palm wine by decoction. The methanol bark extract was found to be more active than other solvents (8 and 512 µg/mL). In fact, the activity of the 6 a.m. extract was significant on 4 of the 35 strains tested (MIC< 100 µg/mL) and moderate on 27 of the 35 strains tested (100≤ MIC≤ 625 µg/mL). The activity of the noon extract was significant on 8 strains and moderate on 23 strains and, finally, the activity of the extract of 6 p.m. was significant on 9 strains and moderate on 25 strains. The extract harvested at 6 p.m. inhibited the growth of the 35 strains tested and revealed significant activity (MIC <100 µg/mL) on Staphylococcus aureus, Escherichia coli, Citrobacter freundii, Serratia marcescens, Salmonella enterica serovar typhi, and Yersinia enterocolitica, making this hour the best harvesting time. This study further confirms that the bark of H. madagascariensis is a potent antibacterial agent, being better still when harvested at 6 p.m. and extracted with methanol.

Does an Optimal Relationship Between Injury Risk and Workload Represented by the "Sweet Spot" Really Exist? An Example From Elite French Soccer Players and Pentathletes.

OBJECTIVE: To examine the relationships between the occurrence and severity of injuries using three workload ratios (ACWR, EWMA, REDI) in elite female soccer players and international male and female pentathletes. MATERIALS AND METHODS: Female soccer players in the U16 to U18 national French teams (n = 24) and international athletes (n = 12, 4 women and 8 men) in the French modern pentathlon team were monitored throughout an entire season. The Acute Chronic Workload Ratio (ACWR), the Exponentially Weighted Moving Averages (EWMA), and the Robust Exponential Decreasing Index (REDI) were calculated for internal load by the ROE method in soccer and external load in pentathlon. The occurrence and severity of injuries (determined according to time-loss) were quantified in the sweet spot zone [0.8; 1.3] and compared to the other zones of load variation: [0; 0.8], [1.3; 1.5], [1.5; +8], using the three ratios. RESULTS: Over the study period, a total of sixty-six injuries (2.75 per athlete) were reported in the soccer players and twelve in pentathletes (1 per athlete). The cumulative severity of all injuries was 788 days lost in soccer and 36 in pentathlon: respectively, 11.9 days lost per injury in soccer player and 3.0 per pentathlete. The mean values across the three methods in soccer showed a higher number of injuries detected in the [0; 0.8] workload ratio zone: 22.3 ± 6.4. They were 17.3 ± 3.5 in the sweet spot ([0.8-1.3] zone) and 17.6 ± 5.5 in the [1.5; +8] zone. In comparison to the [1.5; +8] zone, soccer players reported a higher number of days lost to injuries in the presumed sweet spot and in the [0-0.8] zone: 204.7 ± 28.7 and 275.0 ± 120.7 days, respectively. In pentathletes, ten of the twelve injuries (83.3%) occurred in the presumed sweet spot. REDI was the only method capable of tracking workloads over all-time series. CONCLUSION: In the present cohort of elite soccer players and pentathletes, acute chronic workload calculations showed an association with injury occurrence and severity but did not provide evidence supporting existence of a sweet spot diminishing injury risk.

Robust Exponential Decreasing Index (REDI): adaptive and robust method for computing cumulated workload.

OBJECTIVE: The purpose of this study was to define a new index the Robust Exponential Decreasing Index (REDI), which is capable of an improved analysis of the cumulative workload. This allows for precise control of the decreasing influence of load over time. Additionally, REDI is robust to missing data that are frequently present in sport. METHODS: 200 cumulative workloads were simulated in two ways (Gaussian and uniform distributions) to test the robustness and flexibility of the REDI, as compared with classical methods (acute:chronic workload ratio and exponentially weighted moving average). Theoretical properties have been highlighted especially around the decreasing parameter. RESULTS: The REDI allows practitioners to consistently monitor load with missing data as it remains consistent even when a significant portion of the dataset is absent. Adjusting the decreasing parameter allows practitioners to choose the weight given to each daily workload. DISCUSSION: Computation of cumulative workload is not easy due to many factors (weekends, international training sessions, national selections and injuries). Several practical and theoretical drawbacks of the existing indices are discussed in the paper, especially in the context of missing data; the REDI aims to settle some of them. The decreasing parameter may be modified according to the studied sport. Further research should focus on methodology around setting this parameter. CONCLUSION: The robust and adaptable nature of the REDI is a credible alternative for computing a cumulative workload with decreasing weight over time.

Quantifying Collective Performance in Rugby Union.

Objectives: The aim of this study was to quantify collective experience based on cumulative shared selections of players and to assess its impact on team performance in international rugby union. We assume that the greater the experience, the better the group will perform. Methods: Scoresheets of all games involving at least one of all 10 nations participating at the Rugby Championship and the Six Nations Championship were collected from the end of the 1999 Rugby World Cup (RWC) up to the 2015 RWC. A single indicator quantifying the cumulative shared selections (CSS, the number of selections that each player has shared with the other ones) was computed for each match as a key collective experience indicator. The World Rugby Ranking points of each nation and the percentage of victories were used to estimate team performance. The study period was divided into sequences of 4 years corresponding to the period between two consecutive RWCs. For each sequence and nation, slopes and intercept of CSS trends were computed along with victory percentage and mean ranking points. Multiple linear regression analysis was used to establish the associations between team performance and experience. Results: In regards to the CSS trends, both intra- and inter-nation variability appears to exist. Positive and negative slopes can be observed for the same team from one 4-year cycle to the next. Still, CSS slope is found to be significantly associated with both ranking points (p value = 0.042, R 2 = 0.13) and victory percentage (p value = 0.001, R 2 = 0.42). Conclusion: The evolution of the CSS that quantifies the collective experience of a team is linked to its performance. Such an indicator could be helpful in the decision-making process of national coaching staff.

Prevalence of Bordetella infection in a hospital setting in niamey, niger.

Bordetella pertussis still poses an important health threat in developing countries. In Niger, notified pertussis cases are few despite the low diphtheria-tetanus-pertussis/pentavalent vaccine coverage. We aimed to estimate the prevalence of B. pertussis in children aged <5 years consulting at a pediatric ward. A 5-month study in 2011 recruited 342 children with respiratory symptoms at the National Hospital of Niamey. Nasopharyngeal aspirates were tested by culture and real-time polymerase chain reaction. Overall, 34 (11.2%) of the 305 available nasopharyngeal aspirates tested by real-time polymerase chain reaction were positive for a Bordetella spp., with an estimated prevalence of 8.2 cases per 1000 children aged <5. None was notified to the surveillance network. A single specimen was positive on culture. This study, the first to provide laboratory-confirmed data on pertussis in Niger, highlights the need to sensitize health care personnel to actively notify clinical cases and to integrate laboratory diagnosis in the existing surveillance system.

Modulation of fatty acid transport and metabolism by maternal obesity in the human full-term placenta.

Knowledge of the consequences of maternal obesity in human placental fatty acids (FA) transport and metabolism is limited. Animal studies suggest that placental uptake of maternal FA is altered by maternal overnutrition. We hypothesized that high maternal body mass index (BMI) affects human placental FA transport by modifying expression of key transporters. Full-term placentas were obtained by vaginal delivery from normal weight (BMI, 18.5-24.9 kg/m(2)) and obese (BMI > 30 kg/m(2)) women. Blood samples were collected from the mother at each trimester and from cord blood at delivery. mRNA and protein expression levels were evaluated with real-time RT-PCR and Western blotting. Lipoprotein lipase (LPL) activity was evaluated using enzyme fluorescence. In vitro linoleic acid transport was studied with isolated trophoblasts. Our results demonstrated that maternal obesity is associated with increased placental weight, decreased gestational age, decreased maternal high-density lipoprotein (HDL) levels during the first and third trimesters, increased maternal triglyceride levels during the second and third trimesters, and increased maternal T3 levels during all trimesters, and decreased maternal cholesterol (CHOL) and low-density lipoprotein (LDL) levels during the third trimester; and increased newborn CHOL, LDL, apolipoprotein B100, and T3 levels. Increases in placental CD36 mRNA and protein expression levels, decreased SLC27A4 and FABP1 mRNA and protein and FABP3 protein expression, and increased LPL activity and decreased villus cytotrophoblast linoleic acid transport were also observed. No changes were seen in expression of PPARA, PPARD, or PPARG mRNA and protein. Overall this study demonstrated that maternal obesity impacts placental FA uptake without affecting fetal growth. These changes, however, could modify the fetus metabolism and its predisposition to develop diseases later in life.