Knowledge Enhancement of Elementary School Staff about Traumatic Dental Injuries Using Different Educational Tools.

Introduction: This study aimed to assess the efficacy of different educational tools for knowledge enhancement of elementary school staff about the management of traumatic dental injuries (TDIs). Materials and Methods: This quasi-experimental (pretest-posttest) study was conducted on 126 elementary school staff in Qazvin city, who were randomly selected by the cluster sampling in 2020. The baseline knowledge level of the participants about TDIs was assessed by an online questionnaire. Next, they were randomized into the poster, video clip, and no-intervention control groups (56 samples in each group). An informatory poster and a video clip about TDIs were sent to the participants in the first two groups, respectively, through the WhatsApp instant messaging app, and the knowledge level of the three groups was assessed again after 3 weeks. Data were analyzed by ANOVA, McNemar test, chi-square test, and t-test. Results: A significant correlation was noted between the baseline knowledge level of the participants and their educational level, participation in first aid courses covering TDIs, and history of encountering TDIs (P < 0.05). The knowledge level of the participants significantly increased after the intervention in the poster and video clip groups (P < 0.05). Knowledge enhancement was 43.26% in the poster and 36.61% in the video clip group (P > 0.05). Conclusion: Despite the low-baseline knowledge level of the elementary school staff in Qazvin city about TDIs, their knowledge level significantly improved after the educational interventions.

The emerging role of regulatory cell-based therapy in autoimmune disease.

Autoimmune disease, caused by unwanted immune responses to self-antigens, affects millions of people each year and poses a great social and economic burden to individuals and communities. In the course of autoimmune disorders, including rheumatoid arthritis, systemic lupus erythematosus, type 1 diabetes mellitus, and multiple sclerosis, disturbances in the balance between the immune response against harmful agents and tolerance towards self-antigens lead to an immune response against self-tissues. In recent years, various regulatory immune cells have been identified. Disruptions in the quality, quantity, and function of these cells have been implicated in autoimmune disease development. Therefore, targeting or engineering these cells is a promising therapeutic for different autoimmune diseases. Regulatory T cells, regulatory B cells, regulatory dendritic cells, myeloid suppressor cells, and some subsets of innate lymphoid cells are arising as important players among this class of cells. Here, we review the roles of each suppressive cell type in the immune system during homeostasis and in the development of autoimmunity. Moreover, we discuss the current and future therapeutic potential of each one of these cell types for autoimmune diseases.

VEGFR-3 Expression in Oral Lichen Planus

Background and objective: Given the postulated the role of inflammation and possible contribution of lymphangiogenesis in oral lichen planus, this study aimed to assess any associated presence of VEGFR-3. Material and Methods: This cross-sectional study was performed on 52 formalin fixed and paraffin embedded blocks of oral lichen planus (pathological diagnosis based on Modified WHO criteria), comprising 25 of erosive and 27 of reticular type, along with 60 samples of normal mucosa (with minimal inflammation from clinical and histopathological aspects) obtained at crown lengthening surgery. Four micron sections were cut from paraffin blocks and stained with H and E for confirmation of diagnosis and by immunohistochemistry with primary antibodies against VEGFR-3. Negative controls were provided by omission of primary antibody and placenta was considered as a positive control. Data were analyzed by Chi-square, Mann-Whitney and Kruskal-wallis tests and P <0.05 was considered statistically significant. Findings: VEGFR-3 expression was apparent in 61.5% of lichen planus specimens and 5% of those from normal mucosa (p<0.001). Also, the average number of stained vessels was significantly higher in oral lichen planus than in normal mucosa (p<0.001). VEGFR-3 expression in oral lichen planus (p=0.262) and the average number of stained vessels (p=0.092) demonstrated no significant difference according to the type. Conclusion: It appears that VEGFR-3 expression might be involved in the pathogenesis of the oral lichen planus through increasing lymphatic vessels and lymphangiogenesis.