Analysis of Protection and Immune Response against Teladorsagia circumcincta in Goats Immunised with Thiol-Binding Proteins from Adult Worms.

In view of the increasing occurrence of anthelmintic-resistant strains of gastrointestinal nematodes in ruminants, various alternative control strategies have been investigated, such as those based on the induction of protective immune responses by immunisation with parasite antigens. In this study, the protective activity of somatic antigens from adult worms of Teladorsagia circumcincta purified by affinity chromatography on thiol-sepharose was analysed in goats. After challenge, the enriched products induced a slight reduction in the cumulative faecal egg counts (21%) and in the number of worms (23.3%), with a greater effect on female worms, which also showed a reduction in parameters related to their fertility. These parasitological findings were associated with a Th2 immune response, with a prominent local humoral response and an eosinophilic infiltrate in the gastric mucosa (negatively associated with the fertility of female worms and the number of worms, respectively), as well as an infiltration of MCHII+, CD4+, IgG+ and IgA+ cells. However, several analyses showed an increase in CD8+ cells in the mucosa, as well as IL-2 expression in the gastric lymph nodes, which may have been associated with inhibition of protective responses or with the development of mixed Th1/Th2 responses, a finding that should be analysed in future studies.

Cultural pathways to psychosis care: Patient and caregiver narratives from Puebla, Mexico.

The current study used the McGill Illness Narrative Interview (MINI) to explore patients' (n = 6) and caregivers' (n = 3) narratives about how they identified and sought care for psychosis. Participants were recruited from an outpatient clinic at the Hospital Psiquiátrico Dr. Rafael Serrano, a public psychiatric hospital in Puebla, Mexico. All participants consented to complete semi-structured interviews in Spanish. Thematic analyses were used to inductively identify common themes in participants' narratives. The results indicated that during the initial symptom onset, most participants noticed the presence of hallucinations but did not seek help for this hallmark symptom. Participants described seeking care only when they or their ill relative exhibited escalating aggressive behaviors or physical symptoms that were interpreted as common medical problems. As participants became connected to specialty mental health services, they began to develop a conceptualization of psychosis as a disorder of aggression. For some participants, this conceptualization of psychosis as an illness of aggression contributed to their ambivalence about the diagnosis. These results can be understood using a cultural scripts framework, which suggests that cultural norms are influenced by collective understandings of normalcy and valorization of behaviors. Implications for community campaigns are discussed.

Arabinoxylan source and xylanase specificity influence the production of oligosaccharides with prebiotic potential.

Cereal arabinoxylans (AXs) are complex polysaccharides in terms of their pattern of arabinose and ferulic acid substitutions, which influence their properties in structural and nutritional applications. We have evaluated the influence of the molecular structure of three AXs from wheat and rye with distinct substitutions on the activity of ß-xylanases from different glycosyl hydrolase families (GH 5_34, 8, 10 and 11). The arabinose and ferulic acid substitutions influence the accessibility of the xylanases, resulting in specific profiles of arabinoxylan-oligosaccharides (AXOS). The GH10 xylanase from Aspergillus aculeatus (AcXyn10A) and GH11 from Thermomyces lanuginosus (TlXyn11) showed the highest activity, producing larger amounts of small oligosaccharides in shorter time. The GH8 xylanase from Bacillus sp. (BXyn8) produced linear xylooligosaccharides and was most restricted by arabinose substitution, whereas GH5_34 from Gonapodya prolifera (GpXyn5_34) required arabinose substitution and produced longer (A)XOS substituted on the reducing end. The complementary substrate specificity of BXyn8 and GpXyn5_34 revealed how arabinoses were distributed along the xylan backbones. This study demonstrates that AX source and xylanase specificity influence the production of oligosaccharides with specific structures, which in turn impacts the growth of specific bacteria (Bacteroides ovatus and Bifidobacterium adolescentis) and the production of beneficial metabolites (short-chain fatty acids).

Transfusion-transmission of hepatitis E virus through red blood cell transfusion but not through platelet concentrates: A case report from Spain.

BACKGROUND: Few cases of transfusion-transmitted hepatitis E virus (HEV) have been published in Spain. Here, we describe a well-characterized lookback investigation of a transfusion-transmitted HEV case at the Community Centre for Blood and Tissues of Asturias (Spain). CASE REPORT: A female patient with chronic myeloid leukemia underwent an allogeneic bone marrow transplant in March 2019 and showed alterations in liver function shortly afterwards. This patient received blood components from 30 different donors in the 3 months before the transplant. Frozen plasma samples from these donations were investigated for the presence of HEV-RNA. One frequent donor was identified as asymptomatic HEV RNA-positive at the time of his whole blood donation. The investigation revealed that this donor's plasma unit, originally intended for the fractionation industry, had a viral RNA concentration of 1.9 × 104 copies/mL. HEV RNA was detected initially in the index patient who received the red cell concentrate from this donor 25 days after the transfusion. HEV RNA isolated from both donor and recipient were identified as subtype 3f. The recipient of platelet concentrate (PC), treated with a riboflavin-based pathogen reduction technology (PRT) was not infected, being negative for the presence of HEV IgM, IgG, and HEV RNA before and after the transfusion. CONCLUSION: This case study shows that HEV was transmitted through red cell transfusion to a recipient, while the patient who received riboflavin/UV light treated PC did not develop signs of infection. A causal relationship between PRT treatment of the PC and the non-transmission of HEV remains to be established.

Validation study of the Spanish brief version of TEMPS-A.

INTRODUCTION: Identifying affective temperaments could be useful both for understanding the normal behavioral variations in the general population and to establish if there is a clinical predisposition to certain disorders. Five affective temperaments have been proposed: depressive, cyclothymic, hyperthymic, irritable and anxious. Original instrument for measuring them (TEMPS-A) is a 110-item scale but many short versions in different languages have been validated. The aim of this study was to obtain a short self-administered Spanish version of TEMPS-A with good psychometric properties. MATERIALS AND METHODS: A sample of 550 students who answered the argentinean version of TEMPS-A was included, after psychometric analysis a comparison between inpatients with major depression and their matched controls by sex and age who answered the brief version was performed to get an external validation. RESULTS: The sample was composed by 298 (54.2%) women. The mean age was 23.3 year (SD=6.2). A forced five factor analysis was performed. The 7 items with the highest factorial load (more than 0.350) for each subscale were included in the brief version. The Cronbach alpha's ranged from 0.690 to .800. The most prevalent temperament was hyperthymic followed by cyclothymic for students sample. Similarities between students and controls sample were observed, but not with patients with major depression. CONCLUSIONS: This brief Spanish version of TEMPS-A (35 items) has good psychometric properties and can be used in general and clinical population.

Physical activity in health care professionals as a means of primary prevention of cardiovascular disease: A STROBE compliant cross-sectional study.

ABSTRACT: The aim of this study was to assess the physical activity level of health care professionals, as well as the differences by sex, age, academic background, and among different health care professions.This is an cross-sectional study.Health care settings in the Valencian Community, Spain.A total of 647 health care professionals.Physical activity was assessed with the European Health Interview Survey-Physical Activity Questionnaire (EHIS-PAQ) that includes the assessment of work-related physical activity, transport-related physical activity, health-enhancing physical activity, muscle-strengthening physical activity, and total physical activity.93.51% of all health care professionals were physically active at work. Transport-related physical activity and health-enhancing physical activity were significantly lower in women (21.62% vs 41.86%, P < .001; and 50.19% vs 68.99%, P < .001, respectively). In addition, compliance with health-enhancing and muscle-strengthening physical activity guidelines were lower in older professionals (42.7% vs 61.84%, P < .001; and 47.57% vs 61.84%, P < .001, respectively). Those with higher education were more compliant with health-enhancing and muscle-strengthening physical activity guidelines (58.55% vs 45.69%, P = .002; and 60.24% vs 48.28%, P = .003, respectively). Moreover, 67.98% of physiotherapists performed health-enhancing physical activity and 67.54% muscle-strengthening physical activity regularly, and significant differences in all outcomes were observed compared to the rest of health care professionals (P < .05). Technicians showed lower work-related and total physical activity than nurses and nursing assistants (74.55% vs 90.37%, P = .002; and 83.64% vs 95.72%, P < .001, respectively). Additionally, nursing assistants showed higher work-related physical activity compared to nurses (97.18% vs 90.37%, P = .008).Most health care professionals showed an appropriate level of physical activity. Men performed more transport-related and health-enhancing physical activity than women. Younger professionals and those with higher education were more compliant with health-enhancing and muscle-strengthening physical activity guidelines. Physiotherapists were more physically active when compared to the rest of health care professionals.

Intervenciones para normalizar las actividades de la vida cotidiana en pacientes a los que se ha practicado una reciente ostomía / Interventions to normalize activities of daily living in patients who have had a recent ostomy

CASO: ejemplo mujer de mediana edad procedente de entorno rural a la que se ha practicado una ostomía digestiva definitiva. OBJETIVO: identificar intervenciones basadas en evidencias destinadas a fomentar la autonomía de la persona portadora de un estoma, garantizando su seguridad y motivación hacia el autocuidado. METODOLOGÍA: búsqueda de evidencias siguiendo el modelo PRAXIS. Recomendaciones de buena práctica: (a) colaborar conjuntamente con la paciente en el cuidado del estoma y manejo de dispositivos, (b) para satisfacer las necesidades básicas al descubierto, (c) educar en la prevención de complicaciones, (d) involucrar a la familia y entorno social, (e) apoyarse en el uso de tecnologías visuales y (f) apoyar el establecimiento de relaciones con pares para compartir experiencias. PRÁCTICAS DE AUTOCUIDADO: automanejo del estoma, compartir temores y experiencia con entorno social y grupos de apoyo, y utilización de recursos complementarios

CASE: example of a middle-aged woman from a rural environment who has undergone a definitive digestive ostomy. OBJECTIVE: to identify evidence-based interventions aimed at promoting the autonomy of the person with a stoma, guaranteeing their safety and motivation towards self-care. METHODOLOGY: search for evidence following the PRAXIS model. Good practice recommendations: (a) collaborate together with the patient in the care of the stoma and management of devices, (b) to satisfy the basic needs in the open, (c) educate in the prevention of complications, (d) involve the family and social environment, (e) rely on the use of visual technologies and (f) support the establishment of relationships with peers to share experiences. SELF-CARE PRACTICES: self-management of the stoma, sharing fears and experience with the social environment and support groups, and use of complementary resources

Evaluation of quality of life and depression in patients with rheumatoid arthritis in a General Hospital / Evaluación de la calidad de vida y depresión en pacientes con artritis reumatoide en un hospital general

abstract Introduction: Rheumatoid arthritis (RA) is a chronic, inflammatory, and multisystemic disease that has an impact on the quality of life and is associated with depressive symptoms. Objectives: To evaluate the quality of life, overall health status, and depression in patients with RA. Patients and methods: A cross-sectional study was conducted in the outpatient Rheumatology Outpatient Department of a General Hospital in Mexico City. All patients met the criteria for rheumatoid arthritis according to the American Rheumatism Association, 1987. Clinical and sociodemographic characteristics were recorded. Four measurement tools were used: Arthritis Impact Measurement Scale (AIMS), Dunbar Questionnaire, a Visual Analog Scale, and the Beck Depression Inventory. Results: A total of 43 patients aged ≥ 18 years were evaluated. The highest AIMS scores were in social activity (6.49±1.93), pain (4.70±2.04), depression (4.70±2.23), and physical activity (4.03±2.10). The mean score with the Dunbar questionnaire was 6.48±1.87, the mean Visual Analog Scale score was 69.41±24.67, and the mean of the Beck inventory was 15.74±9.85. Conclusions: The presence of anxiety and depression has an impact on the quality of life of patients with RA, but not on the presence of comorbidity or disease activity. Depressive symptoms are common in patients with RA, and should therefore be considered when evaluating this rheumatological disease.

resumen Introducción: La artritis reumatoide (AR) es una enfermedad que afecta la calidad de vida de quienes la padecen y se asocia a sintomatologia depresiva. Objetivos: Evaluar la calidad de vida, el estado global de salud y depresión en pacientes con AR. Pacientes y métodos: Se trata de un estudio transversal y comparativo. Se incluyeron 43 adultos mayores, ≥ de 18 anos, con diagnóstico de AR según los criterios de la American Rheumatism Association de 1987, que acudían al servicio de consulta externa de Reumatologia de un hospital general en la ciudad de México. Se determinaron características clínicas y sociodemográficas. Se les aplicaron 4 índices: Escala de Medición del Impacto de la Artritis (AIMS), Cuestionario de Dunbar, Escala Análoga Visual y el Inventario de Depresión de Beck. Resultados: Se evaluaron 43 pacientes mayores de 18 anos, en su mayoría del sexo femenino. Para la AIMS, las puntuaciones más altas fueron en actividad social (6, 49 ± 1, 93), dolor (4,70 ± 2,04), depresión (4,70 ± 2,23) y actividad física (4,03 ± 2,10). Para el cuestionario de Dunbar la puntuación promedio fue de 6, 48 ± 1, 87, en la escala análoga visual la puntuación media fue de 69,41 ± 24,67, finalmente, en el inventario de Beck el promedio fue de 15,74 ±9,85. Conclusiones: La presencia de ansiedad y depresión se relacionan con la calidad de vida de los pacientes con AR mas no con la presencia de comorbilidad o actividad de la enfermedad. Los síntomas depresivos son comunes en pacientes con AR por lo que deben considerarse al evaluar esta enfermedad reumatológica.

In silico genomic insights into aspects of food safety and defense mechanisms of a potentially probiotic Lactobacillus pentosus MP-10 isolated from brines of naturally fermented Aloreña green table olives.

Lactobacillus pentosus MP-10, isolated from brines of naturally fermented Aloreña green table olives, exhibited high probiotic potential. The genome sequence of L. pentosus MP-10 is currently considered the largest genome among lactobacilli, highlighting the microorganism's ecological flexibility and adaptability. Here, we analyzed the complete genome sequence for the presence of acquired antibiotic resistance and virulence determinants to understand their defense mechanisms and explore its putative safety in food. The annotated genome sequence revealed evidence of diverse mobile genetic elements, such as prophages, transposases and transposons involved in their adaptation to brine-associated niches. In-silico analysis of L. pentosus MP-10 genome sequence identified a CRISPR (clustered regularly interspaced short palindromic repeats)/cas (CRISPR-associated protein genes) as an immune system against foreign genetic elements, which consisted of six arrays (4-12 repeats) and eleven predicted cas genes [CRISPR1 and CRISPR2 consisted of 3 (Type II-C) and 8 (Type I) genes] with high similarity to L. pentosus KCA1. Bioinformatic analyses revealed L. pentosus MP-10 to be absent of acquired antibiotic resistance genes, and most resistance genes were related to efflux mechanisms; no virulence determinants were found in the genome. This suggests that L. pentosus MP-10 could be considered safe and with high-adaptation potential, which could facilitate its application as a starter culture and probiotic in food preparations.

Humoral immune responses of experimentally Eimeria ninakholyakimovae-infected goat kids.

Although cellular immune reactions seem to be crucial for protective immune responses in Eimeria spp. infections, there are also evidences on an active involvement of the humoral counterpart. In the present study, we have analyzed the humoral response of goat kids subjected to primary and challenge infections with Eimeria ninakholyakimovae. Specific levels of IgG and IgM in serum samples and IgA in the ileal mucus were estimated. In infected kids, significantly increased levels of IgG were observed from 3 weeks post infection onwards in addition to an enhancement of specific IgM and secretory IgA levels. A wide range of peptides of sporulated oocyst antigen (SOA) was recognized by specific IgG as determined by immunoblotting. However, no correlations were found between immunoglobulin levels and OPG counts after challenge infection. Overall, these data indicate a significant specific humoral response of E. ninakohlyakimovae-infected goat kids that does not seem to convey immunoprotection. Further studies should be addressed to clarify if the lack of correlation might be associated to the type of antigen used for the immunoenzimatic assays, the age of the animals or other factors.

Complete Genome Sequence of a Potential Probiotic, Lactobacillus pentosus MP-10, Isolated from Fermented Aloreña Table Olives.

We report here a 3,698,214-bp complete genome sequence of a potential probiotic Lactobacillus pentosus strain, MP-10, isolated from brines of naturally fermented Aloreña green table olives; it is considered the largest sequenced genome among lactobacilli to date. The annotated genome sequence revealed the presence of 3,558 open reading frames (ORFs) and 87 structural RNAs.

Biocide tolerance, phenotypic and molecular response of lactic acid bacteria isolated from naturally-fermented Aloreña table to different physico-chemical stresses.

Lactic acid bacteria (LAB) isolated throughout the fermentation process of Aloreña table olives were found to be resistant at least to three antibiotics (Casado Muñoz et al., 2014); however, most were sensitive to the biocides tested in this study (with minimum inhibitory concentrations [MIC] below the epidemiological cut-off values). 2-15% of the isolates were found to be biocide resistant: Leuconostoc Pseudomesenteroides, which were resistant to hexachlorophene, and Lactobacillus pentosus to cetrimide and hexadecylpiridinium. We analyzed the effect of different physico-chemical stresses, including antimicrobials, on the phenotypic and genotypic responses of LAB, providing new insights on how they become resistant in a changing environment. Results indicated that similar phenotypic responses were obtained under three stress conditions: antimicrobials, chemicals and UV light. Susceptibility patterns to antibiotics changed: increasing MICs for ampicillin, chloramphenicol, ciprofloxacin, teicoplanin and tetracycline, and decreasing the MICs for clindamycin, erythromycin, streptomycin and trimethoprim in most strains. Statistically, cross resistance between different antibiotics was detected in all stress conditions. However, expression profiles of selected genes involved in stress/resistance response (rpsL, recA, uvrB and srtA) differed depending on the stress parameter, LAB species and strain, and the target gene. We conclude that, despite the uniform phenotypic response to stresses, the repertoire of induced and repressed genes differs. So, a search for a target to improve stress tolerance of LAB, especially those of importance as starter/protective cultures or probiotics, may depend on the individual screening of each strain, even though we could predict the antibiotic phenotypic response to all stresses.

Comparative proteomic analysis of a potentially probiotic Lactobacillus pentosus MP-10 for the identification of key proteins involved in antibiotic resistance and biocide tolerance.

Probiotic bacterial cultures require resistance mechanisms to avoid stress-related responses under challenging environmental conditions; however, understanding these traits is required to discern their utility in fermentative food preparations, versus clinical and agricultural risk. Here, we compared the proteomic responses of Lactobacillus pentosus MP-10, a potentially probiotic lactic acid bacteria isolated from brines of naturally fermented Aloreña green table olives, exposed to sub-lethal concentrations of antibiotics (amoxicillin, chloramphenicol and tetracycline) and biocides (benzalkonium chloride and triclosan). Several genes became differentially expressed depending on antimicrobial exposure, such as the up-regulation of protein synthesis, and the down-regulation of carbohydrate metabolism and energy production. The antimicrobials appeared to have altered Lb. pentosus MP-10 physiology to achieve a gain of cellular energy for survival. For example, biocide-adapted Lb. pentosus MP-10 exhibited a down-regulated phosphocarrier protein HPr and an unexpressed oxidoreductase. However, protein synthesis was over-expressed in antibiotic- and biocide-adapted cells (ribosomal proteins and glutamyl-tRNA synthetase), possibly to compensate for damaged proteins targeted by antimicrobials. Furthermore, stress proteins, such as NADH peroxidase (Npx) and a small heat shock protein, were only over-expressed in antibiotic-adapted Lb. pentosus MP-10. Results showed that adaptation to sub-lethal concentrations of antimicrobials could be a good way to achieve desirable robustness of the probiotic Lb. pentosus MP-10 to various environmental and gastrointestinal conditions (e.g., acid and bile stresses).

The History of Spanish Nursing (1953-1980): introductory notes / Historia de la Enfermería Española (1953-1980): notas introductorias / História da Enfermagem Espanhola (1953-1980): notas introdutórias

ABSTRACT A historical-social documentary study aiming to analyze the history of Nursing in Spain, in the period 1953 - 1980. Undertaken between September and December 2014 through consultations with archive services, newspapers, legislation, specialized journals and academic works. This historical period corresponds to the unification of the teaching of practitioners, midwives and nurses (1953) and to the approval of the standards of the title of Sanitary Technical Assistant with Diploma in Nursing (1980). Thematic analysis was used for understanding the meanings and thus to (re-)construct part of this history. The empirical material revealed that the Real Escuela de Enfermeras de Santa Isabel de Hungria is considered the pioneer within the anglo-saxon model, that Spanish Nursing results from the fusion of three professions (practitioners, midwives and nurses) and training separated by sex was striking. Finally, it is concluded that the trajectory of Spanish Nursing towards the level of a graduate profession was long and arduous.

RESUMEN Investigación documental y socio-histórica que objetivó analizar la historia de la Enfermería en España de 1953 a 1980. Fue elaborado entre septiembre y diciembre de 2014, a través de consultas realizadas en los servicios de archivos, periódicos, legislaciones, revistas especializadas y trabajos académicos. El recorte histórico inicial, corresponde a la unificación de las enseñanzas de los practicantes, matronas y enfermeras (1953) y el final a la homologación de las normas de título de Ayudante de Técnico Sanitario con Diploma en Enfermería (1980). Se utilizó análisis temático para la comprensión criteriosa y sistémica de los significados, para así (re)construir parte de esa historia. El material empírico reveló que la Real Escuela de Enfermeras de Santa Isabel de Hungría es considerada la pionera en el modelo anglosajón; la Enfermería española resulta de la fusión de tres profesiones (practicantes, matronas y enfermeras) y que fue significativa la formación separada por sexo. Finalmente, se concluye que la trayectoria de la Enfermería española fue larga para llegar al nivel superior.

RESUMO Estudo histórico-social documental com o objetivo de analisar a história da Enfermagem na Espanha, no período de 1953 a 1980. Elaborado entre setembro e dezembro de 2014 através de consultas aos serviços de arquivos, jornais, legislação, revistas especializadas e trabalhos acadêmicos. O recorte histórico corresponde à unificação dos ensinos de praticantes, matronas e enfermeiras (1953) à homologação das normas do título de Ajudante Técnico Sanitário em Diplomado em Enfermagem (1980). Utilizou-se a análise temática para compreensão dos significados e assim (re)construir parte dessa história. O material empírico revelou que a Real Escuela de Enfermeras de Santa Isabel de Hungria é considerada a pioneira no modelo anglo-saxão, que a Enfermagem espanhola resulta da fusão de três profissões (praticantes, matronas e enfermeiras) e que foi marcante a formação separada por sexo. Por fim, conclui-se que longa e árdua foi a trajetória da Enfermagem espanhola rumo ao patamar de profissão de nível superior.

Actitudes hacia la homoparentalidad: Validación psicométrica de dos escalas en una muestra de estudiantes mexicanos / Atitudes em relação à homoparentalidade: validação psicométrica de duas escalas numa amostra de estudantes mexicanos / Attitudes Towards Homoparentality: Psychometric Validation of two Scales in a Sample of Mexican Students

Introducción: La legalización del llamado matrimonio igualitario, y de la adopción de menores por parejas homosexuales, es un fenómeno reciente y que debe ser estudiado, para lo cual es necesario contar con medidas confiables que permitan dar cuenta de cómo la sociedad percibe esta nueva configuración familiar. Objetivo: Determinar las propiedades psicométricas de dos escalas: Actitudes frente a las familias Homoparentales (AFFH); y creencias acerca del ajuste de los niños de familias homoparentales (CANFH). Metodología: La AFFH, con 20 reactivos, fue diseñada para dar cuenta de las actitudes hacia las familias homoparentales. La CANFH tiene 14 reactivos organizados en las subescalas de oposición individual (OI) y oposición normativa (ON). Ambas escalas tienen afirmaciones que deben ser respondidas con opciones tipo Likert que van del 1 totalmente de acuerdo, al 5 totalmente en desacuerdo. La CANFH fue aplicada a 170 estudiantes universitarios (78 (46%) hombres, 92 (54%) mujeres. Media de edad: 18.4 DE+0.94), y la AFFH a 88 (35 (40%) hombres, 53 (60%) mujeres. Media de edad: 18.2 DE+0.84). Se determinaron las propiedades psicométricas por medio de análisis factorial y de consistencia interna. Resultados: La AFFH resultó con un α=0.91; los reactivos se organizaron en dos factores que explican 46.14% de la varianza. En el caso de la CANFH se confirmó la estructura factorial de dos subescalas explicativas de 65.49% de la varianza y con un α=0.94. Conclusiones: Los datos indican que ambas escalas poseen las propiedades psicométricas adecuadas para medir las actitudes de estudiantes mexicanos hacia las familias homoparentales y hacia los efectos que estas puedan tener en los menores adoptados...(AU)

Introduction: Same-sex marriage and adoption legalization constitute a growing and relatively new research field. Its study needs reliable and valid measures that allow to understand the society's perception about this new family order. Objective: Assess the psychometric properties of two scalesdevised to measure same-sex parenting attitudes: Attitudes toward Same-sex Families Scale (AFFH for its acronym in Spanish), and Scale on Beliefs about Children's Adjustment in Same-sex families (CANFH in Spanish). Methodology: AFFH scale is a 20-item measure designed to account for attitudes towards same-sex families. CANFH is a 14-item measure consisting of two scales, Individual Opposition (IO) and Normative Opposition (NO). Both are Likert scales with five response options (1. Strongly Agree to 5. Strongly Disagree). The CANFH was answered by 170 college students (78 (46%) males, 92 (54%) females; Average age: 18.4 SD 0.94), and the AFFH by 88 college students (35 (40%) males, 53 (60%) females, average age: 18.2 SD + 0.84). Results: AFFH items were organized into two factors that explained 46.14% of variance with a Cronbach's alpha of 0.91. CANFH items were grouped in a two factors structure that explained 65.49% of the variance (α = 0.94). Conclusions: CANFH and AFFH scales have adequate psychometric properties to measure Mexican college students' attitudes toward same-sex families and to assess the beliefs about children's adjustment in same-sex families...(AU)

Introdução: A legalização do chamado casamento do mesmo sexo e adoção de crianças por casais homossexuais, é um fenômeno recente, que deve ser estudado, para a qual é necessário ter medidas confiáveis que permitem ver como a sociedade percebe esta nova configuração familiar. Objetivo: determinar as propriedades psicométricas de duas escalas: Atitudes para com as famílias homoparentais (AFFH); e as crenças sobre o equilíbrio das crianças de famílias homoparentais. Metodologia: O AFFH, com 20 itens, foi projetado para explicar atitudes das famílias homoparentais. O CANFH tem 14 reagentes organizados em sub-escalas de oposição individual (OI) e oposição regulamentar (ON). Ambas as escalas são declarações que devem ser respondidas com opções de tipo Likert, entre 1 que significa estar de acordo e 5 que discorda totalmente (CANFH). O CANFH foi aplicado a 170 estudantes universitários (78 (46%) do sexo masculino, 92 (54%) mulheres com idade média: 18.4 + 0.94), e AFFH a 88 (35 (40%) do sexo masculino, 53 (60% ) mulheres média de idade. 18.2 + 0.84). Foram determinadas as propriedades psicométricas por meio de análise fatorial e consistência interna. Resultados: A AFFH foi α = 0.91 com reagentes foram organizados em dois fatores que explicam 46.14% da variância. No caso de a estrutura fatorial CANFH dois explicativa 65.49% da variância e um α = 0,94 subescalas foi confirmada. Conclusões: Os dados indicam que ambas as escalas têm o direito de medir as atitudes dos estudantes em relação homoparentais famílias mexicanas e para os efeitos que podem ter sobre as crianças adotadas propriedades psicométricas...(AU)

THE BIRTH OF NURSING AT THE INCEPTION OF THE FRANCOISM IN SPAIN (1939-1942) / O NASCIMENTO DA ENFERMAGEM DURANTE O INÍCIO DO FRANQUISMO NA ESPANHA (1939-1942) / EL NACIMIENTO DE LA ENFERMERÍA DURANTE LOS INICIOS DEL FRANQUISMO EN ESPAÑA (1939-1942)

ABSTRACT This study is aimed at analyzing the creation of the Nursing science in the early days of the Franco regime in Spain (1939-1942). This was a qualitative and historical study, that used as documentary sources, printed newspapers in different regions of Spain. Results. The importance of the work of nursing during the state of need, hunger, misery, for the post-war Spanish population was identified. Nurses with this attitude and commitment to one another, facilitated the administration of food for the needy, stocked up on clothes for those who were suffering from cold, and taught hygiene principles to mothers in order to reduce the high mortality rate of children and youth who was suffering due to the performance of social assistance. Therefore, in the face of adversity, nurses attended and cared for the children, sick, elderly and the poor. It is concluded that the humanitarian work of nurses became an element of repression, distributing benefits in exchange for an ideological model.

RESUMO Estudo com o objetivo de analisar o nascimento da enfermagem sob o início de Franco na Espanha (1939-1942). Estudo histórico qualitativo documental, cujas fontes utilizadas foram os jornais impressos em diferentes regiões da Espanha, no período 1939-1942. Os resultados revelaram a importância do trabalho da enfermagem no estado de necessidade, fome, miséria para a população espanhola após a guerra. A atitude e compromisso dos enfermeiros com o outro facilitaram os cuidados aos necessitados em termos de alimentação, roupas e ensino de princípios de higiene para as mães a fim de reduzir a elevada taxa de mortalidade de crianças e jovens necessitados de assistência social. Portanto, diante da adversidade, enfermeiros participaram dos cuidados para as crianças, os doentes, os idosos e os pobres. Concluiu-se que o trabalho humanitário dos enfermeiros tornou-se um elemento de benefícios repressão distribuição em troca da exigência de um modelo ideológico.

RESUMEN Estudio con el objetivo de analizar el nacimiento de la Enfermería en el primer franquismo en España (1939-1942). Estudio histórico cualitativo, cuyas fuentes documentales utilizadas fueron los periódicos impresos en diferentes regiones de España, en el periodo 1939-1942. La importancia del trabajo de los cuidados de enfermería en el estado de necesidad, hambre, miseria, en que había quedado la población española tras el conflicto bélico. Las enfermeras con su actitud y compromiso hacia el otro, facilitaron la administración de alimentos a los más necesitados, abastecieron de ropas a los que padecían frio y enseñaron principios de higiene a las madres para reducir la elevada tasa de mortalidad infantil y juvenil que se padecía, puntos cruciales para la actuación de la ayuda social. Por tanto, ante la adversidad, las enfermeras atendieron y cuidaron a los niños, a los enfermos, a los ancianos y a los pobres. Se concluye que la labor humanitaria de las enfermeras se convertía en un elemento de represión distribuyendo prestaciones a cambio de la exigencia de un modelo ideológico.

The controversial nature of the Weissella genus: technological and functional aspects versus whole genome analysis-based pathogenic potential for their application in food and health.

Despite the use of several Weissella (W.) strains for biotechnological and probiotic purposes, certain species of this genus were found to act as opportunistic pathogens, while strains of W. ceti were recognized to be pathogenic for farmed rainbow trout. Herein, we investigated the pathogenic potential of weissellas based on in silico analyses of the 13 whole genome sequences available to date in the NCBI database. Our screening allowed us to find several virulence determinants such as collagen adhesins, aggregation substances, mucus-binding proteins, and hemolysins in some species. Moreover, we detected several antibiotic resistance-encoding genes, whose presence could increase the potential pathogenicity of some strains, but should not be regarded as an excluding trait for beneficial weissellas, as long as these genes are not present on mobile genetic elements. Thus, selection of weissellas intended to be used as starters or for biotechnological or probiotic purposes should be investigated regarding their safety aspects on a strain to strain basis, preferably also by genome sequencing, since nucleotide sequence heterogeneity in virulence and antibiotic resistance genes makes PCR-based screening unreliable for safety assessments. In this sense, the application of W. confusa and W. cibaria strains as starter cultures or as probiotics should be approached with caution, by carefully selecting strains that lack pathogenic potential.

Correlation between antibiotic and biocide resistance in mesophilic and psychrotrophic Pseudomonas spp. isolated from slaughterhouse surfaces throughout meat chain production.

The aim of this study was to evaluate biocide susceptibility in mesophilic and psychrotrophic pseudomonads isolated from surfaces of a goat and lamb slaughterhouse, which was representative of the region. To determine biocide resistance in pseudomonads, we determined for the first time the epidemiological cut-off values (ECOFFs) of benzalkonium, cetrimide, chlorhexidine, hexachlorophene, P3 oxonia, polyhexamethylene guanidine hydrochloride (PHMG), topax 66 and triclosan being generally very similar in different Pseudomonas spp. with some exceptions. Thus, resistance of pseudomonads was mainly shown to triclosan, and in lesser extent to cetrimide and benzalkonium chloride depending on the species, however they were highly susceptible to industrial formulations of biocides. By means of statistical analysis, positive correlations between antibiotics, biocides and both antimicrobials in pseudomonads were detected suggesting a co- or cross resistance between different antimicrobials in goat and lamb slaughterhouse environment. Cross-resistance between biocides and antibiotics in pseudomonads were especially detected between PHMG or triclosan and different antibiotics depending on the biocide and the population type. Thus, the use of those biocides as disinfectant in slaughterhouse zones must be carefully evaluated because of the selection pressure effect of antimicrobials on the emergence of resistant bacteria which could be spread to the consumer. It is noteworthy that specific industrial formulations such as topax 66 and oxonia P3 showed few correlations with antibiotics (none or 1-2 antibiotics) which should be taken into consideration for disinfection practices in goat and lamb slaughterhouse.

Intervención en el servicio de urgencias para lograr un inicio y seguimiento adecuados de la anticoagulació en pacientes con fibrilación auricular / Emergency department training on the appropriate prescription of anticoagulants in atrial fibrillation and on patient follow-up after discharge

Objetivos: Evaluar el resultado de una intervención formativa realizada en el servicio de urgencias (SU), en cuanto a la correcta prescripción y uso de un protocolo de anticoagulación en pacientes con fibrilación auricular (FA) y su posterior seguimiento. Método: Estudio cuasi-experimental con un grupo de control no equivalente, pre/post intervención formativa. Se seleccionaron dos grupos de pacientes (pre y postintervención) mediante muestreo consecutivo, mayores de 18 años, que se presentaron en el SU con FA identificada en el ECG y con criterios de anticoagulación según la estratificación de riesgo de la escala CHA2DS2-VASc 􀀀 2. El resultado primario fue la proporción de pacientes anticoagulados al alta del SU. El resultado secundario fue la proporción de pacientes anticoagulados al mes del alta del SU. Resultados: Se incluyeron 184 pacientes (86 preintervención, 98 postintervención). La intervención aumentó de manera significativa el comienzo de la anticoagulación [preintervención 32 (37%) vs postintervención 95 (97%);p < 0,001] y el seguimiento al mes de comenzar el tratamiento [preintervención 26 (30%) vs postintervención 91(93%); p < 0,001]. Conclusiones: Una intervención formativa en el SU es efectiva para implementar los protocolos adaptados a las guías de práctica clínica de la anticoagulación (AU)

Objectives: The main objective was to evaluate the effect of an emergency department training intervention on the use of a protocol for prescribing anticoagulant therapy in atrial fibrillation and on following patients after discharge. Methods: Quasi-experimental pre-post study of a training intervention; the control patients were not matched but were selected consecutively before and after the intervention. All patients were over the age of 18 years, had atrialfibrillation identified by electrocardiogram, and indications for anticoagulant therapy, specifically a score of 2 or more on the CHADS2-DS2-VASc scale (cardiac failure, hypertension, age >75 years, diabetes, stroke [doubled], vascular disease, age 65-74 years, sex). The primary outcome was percentage of patients on anticoagulant therapy on discharge from the department. The secondary outcome was the percentage of patients on therapy 1 month after discharge. Results: A total of 184 patients (86 before the intervention and 98 afterwards) were included. Training significantly increased the prescription of anticoagulants in the department (before the intervention, 32 patients [37%] vs 95[97%] afterwards; P<.001). In the preintervention period, 26 patients (30% were on anticoagulants 1 month later; in the postintervention period, 26 (30%) were still on therapy (P<.001). Conclusions: This study shows that emergency staff training is effective for implementing protocols based on anticoagulant therapy guidelines (AU)