ABSTRACT
CASE: We reported a dynamic neurogenic left thoracic outlet syndrome (TOS) with a permanent abduction of the fifth left finger. Preoperative magnetic resonance imaging (MRI) assessed the presence of subclavius posticus muscle (SPM). Because of a nonoperative treatment failure, we performed a brachial plexus neurolysis and SPM resection. Immediate postoperative assessment showed an immediate disappearance of the Wartenberg sign. CONCLUSION: SPM constitutes an underestimated cause of TOS. A careful MRI reading is necessary to make correct diagnosis. Nonoperative treatment includes physiotherapy and can be proposed in first instance. When nonoperative treatment fails, brachial plexus exploration with release of the SPM may result in resolution of symptoms.
Subject(s)
Brachial Plexus , Thoracic Outlet Syndrome , Brachial Plexus/diagnostic imaging , Brachial Plexus/surgery , Humans , Muscle, Skeletal/diagnostic imaging , Muscle, Skeletal/surgery , Pectoralis Muscles , Shoulder , Thoracic Outlet Syndrome/diagnostic imaging , Thoracic Outlet Syndrome/surgeryABSTRACT
We report on the loading and release behaviors of polyelectrolyte multilayers based on hydrophobically modified carboxymethylpullulan (CMP) derivatives and poly(ethyleneimine) (PEI) toward hydrophobic dye. The dye-loaded films are obtained according to two different protocols: (i) the postdiffusion approach, which involves the diffusion of the dye within preassembled self-assemblies, and (ii) the precomplexation method, which requires the formation of a water-soluble amphiphilic CMP derivative-dye complex before the multilayer buildup. In both cases, we provide clear evidence for the entrapment of the dye in hydrophobic nanoreservoirs resulting from the aggregation of decyl pendent groups grafted on CMP chains. We show that the loading capacity of the multilayers, as well as their release behavior, can be tuned by varying the grafting degree of CMP chains. Moreover, we demonstrate the possibility to trigger the subsequent release of the loaded dye molecules by varying the composition of the surrounding solution.
Subject(s)
Polysaccharides/chemistry , Electrolytes , Fluorescent Dyes , Glucans/chemistry , Hydrophobic and Hydrophilic Interactions , Microscopy, Atomic Force , Oxazines , Polyethyleneimine/chemistry , Surface PropertiesABSTRACT
We describe a unique case of a patient presenting with rectal impaction following self-administration of a liquid used as masonry adhesive for anal sexual gratification. The solidified matter required laparotomy for its removal. Strategies for removing rectal foreign bodies are discussed as well as other consequences of inserting foreign material per rectum.
Subject(s)
Epoxy Resins/adverse effects , Fecal Impaction/chemically induced , Foreign Bodies/surgery , Paraphilic Disorders/complications , Rectum , Adult , Follow-Up Studies , Foreign Bodies/diagnostic imaging , Humans , Male , Radiography , Risk Assessment , Treatment OutcomeABSTRACT
The regenerating activities of chemically modified anionic bacterial polysaccharides by O-sulfonation were investigated using a in vivo model of rat injured muscle regeneration. Glucuronan (GA), a linear homopolysaccharide of -->4)-beta-D-GlcpA-(1--> residues partially acetylated at the C-3 and/or the C-2 position, and glucoglucuronan (GGA), a linear heteropolysaccharide of -->3)-beta-D-GlcpA-(1-->4)-beta-D-Glcp-(1--> residues were sulfated. SO3-DMF sulfatation complex provided polysaccharides with different sulfur contents, however, a depolymerization occurred because we did not use large excess of pyridine to obtain pure modified polysaccharides. A regenerating activity on injured extensor digitorum longus (EDL) muscles on rats was obtained with these two sulfated anionic polymers. The position of sulfate groups on glucoglucuronan (primary or secondary alcohol) seems to have no influence on the biological activity by opposition to the degree of sulfatation both for the glucuronans and the glucoglucuronans. The yield of acetate groups in the glucuronan polymer modulated the specific activity.
Subject(s)
Muscle, Skeletal/physiology , Polysaccharides, Bacterial/chemistry , Polysaccharides, Bacterial/pharmacology , Regeneration/drug effects , Rhizobium , Sinorhizobium meliloti , Acetylation , Animals , Anions , Glucuronates/chemistry , Glucuronates/pharmacology , Male , Muscle, Skeletal/drug effects , Rats , Rats, Wistar , Regeneration/physiology , Sulfates/chemistry , Sulfates/metabolismABSTRACT
The aim of this study was to develop new surfactants for membrane protein solubilization, from a natural, biodegradable polymer: the polysaccharide pullulan. A set of amphiphilic pullulans (HMCMPs), differing in hydrophobic modification ratio, charge ratio, and the nature of the hydrophobic chains introduced, were synthesized and tested in solubilization experiments with outer membranes of Pseudomonas fluorescens. The membrane proteins were precipitated, and then resolubilized with various HMCMPs. The decyl alkyl chain (C(10)) was the hydrophobic graft that gave the highest level of solubilization. Decyl alkyl chain-bearing HMCMPs were also able to extract integral membrane proteins from their lipid environment. The best results were obtained with an amphiphilic pullulan bearing 18% decyl groups (18C(10)). Circular dichroism spectroscopy and membrane reconstitution experiments were used to test the structural and functional integrity of 18C(10)-solubilized proteins (OmpF from Escherichia coli and bacteriorhodopsin from Halobacterium halobium). Whatever their structure type (alpha or beta), 18C(10) did not alter either the structure or the function of the proteins analyzed. Thus, HMCMPs appear to constitute a promising new class of polymeric surfactants for membrane protein studies.
