ABSTRACT
Considering the antioxidant activity of the Trichilia catigua extract (TCE), the aim of the current study was to develop and characterize W/O/W multiple emulsions containing different vegetable oils as a platform to deliver a TCE. The extract displayed antioxidant activity (IC50) of 4.59 µg/mL and total phenol content (TPC) of 50.84%. Formulations were prepared by the phase-inversion emulsification method and analyzed for morphological appearance, pH, conductivity, droplet size and distribution, content of active, rheological properties, in vitro release, skin permeation, and stability. Formulations prepared with canola oil were selected and displayed regular morphology, mean diameter 2.77 µm (without TCE), 3.07 µm with 0.5% and 3.23 µm with 1.0% TCE. Rheometry (flow) showed pseudoplastic behavior with minimal thixotropy for both systems. TCE could be released from emulsions containing 1.0% and 0.5% TCE in a controlled manner for 16 and 23 h, respectively. The emulsions allowed good retention of TCE in the skin (stratum corneum, epidermis, and dermis). In a 180-d assessment of accelerated chemical stability, TPC was more reduced for the emulsions at 40 °C; other parameters remained stable. Multiple emulsions containing TCE were developed, exhibited good characteristics, and may be considered for future investigations as anti-aging formulations for the skin.
Subject(s)
Delayed-Action Preparations/chemistry , Emulsions/chemistry , Meliaceae/chemistry , Animals , Antioxidants/chemistry , Chemistry, Pharmaceutical/methods , Drug Liberation , Drug Stability , Particle Size , Permeability , Plant Oils/chemistry , Rapeseed Oil , Rheology , Skin/metabolism , Skin Absorption , Swine , Water/chemistryABSTRACT
Tagetes patula L. (Asteraceae), popularmente conhecida como cravo de defunto ou cravo francês, é utilizada na medicina popular como antisséptica, depurativa do sangue e repelente de insetos. O presente estudo avaliou a atividade antisséptica de sabonete líquido contendo extrato glicólico de T. patula 7,5% (p/v). O extrato glicólico foi obtido por turbo-extração utilizando propilenoglicol e etanol (7:3; v/v) a 10 % (p/v). A avaliação fitoquímica preliminar da droga vegetal indicou a presença de compostos fenólicos e teor de flavonoides de 5,73% ± 0,19 (CV = 3,33%). No teste para avaliação da atividade antimicrobiana houve a inibição do crescimento de Staphylococcus aureus na concentração de avaliada.
Tagetes patula L. (Asteraceae), or French marigold, known in Brazil as cravo-de-defunto, is used in folk medicine as antiseptic, blood thinner and insect repellent. Current study analyzes the antiseptic activity of the liquid soap with the glycolic extract of T.patula 7.5% (p/v). Glycolic extract was obtained by turbo-extraction with propylenoglycol and ethanol (7:3; v/v) 10 % (p/v). Preliminary photochemical evaluation of the vegetal drug showed phenolic compounds and flavonoid rates at 5.73% ± 0.19 (CV = 3.33%). When the pharmaceutical formulation was evaluated for antimicrobial activity, growth inhibition of Staphylococcus aureus occurred at the assessed concentration.
Subject(s)
Flavonoids , Anti-Infective Agents, Local , Pharmaceutical Preparations , Phenolic Compounds , PhytochemicalsABSTRACT
Sabe-se que o número de casos de câncer de pele tem crescido muito nos últimos anos. Com isso, novos produtos são constantemente desenvolvidos no intuito de aumentar a gama de substâncias para proteção solar. O principal objetivo desse trabalho foi a análise in vitro da propriedade fotoprotetora de uma formulação contendo filtros solares químicos, adicionada de extratos brutos de Guazuma ulmifolia, Maytenus ilicifolia, Stryphnodendron adstringens e Trichilia catigua, respectivamente, e a avaliação quanto ao acréscimo do Fator de Proteção Solar (FPS). O ensaio foi conduzido pelo método espectrofotométrico in vitro do Fator de Proteção Solar (FPS). O teor de Polifenóis Totais (PT) e sua correlação com a Capacidade Antioxidante (CA) dos extratos avaliados foram realizados. Os resultados demonstram que as formulações foram estáveis após a adição dos extratos, quanto a características físico-químicas (características macroscópicas, ensaio de centrifugação e pH) quando comparadas ao controle. O ensaio de FPS in vitro demonstrou decaimento do FPS das formulações acrescidas de extratos vegetais ricas em compostos fenólicos, exceto para o extrato de S. adstringens. Os FPS obtidos para os extratos foram ≤2. O teor em PT foram (%): G. ulmifolia, 24,26±0,34; M. ilicifolia, 14,66±0,18; S. adstringens, 34,38±0,62 e T. catigua, 41,60±0,13. Os valores da CA (IC50) frente ao radical DPPH foram (miug/mL): vitamina C (7,52±0,12), G. ulmifolia (8,94±0,13), M. ilicifolia (38,41±0,93), S. adstringens (7,31±0,15) e T. catigua (5,48±0,04). A CA foi diretamente proporcional ao teor de PT.
It is known that the number of cases of skin cancer has increased greatly in recent years. Therefore, new products are constantly developed in order to increase the range of substances for sun protection. The aim of this study was evaluate in vitro the property the sunscreen containing chemical sunscreens, with added to extracts of Guazuma ulmifolia, Maytenus ilicifolia, Stryphnodendron adstringens and Trichilia catigua, Sun Protection Factor (SPF). The test was conducted by the spectrophotometric method of in vitro Sun Protection Factor (SPF). The percentage of the Total Polyphenol (TP) and its correlation with Antioxidant Capacity (CA) of the extracts evaluated were performed. The results show that the formulations were stable after the addition of extracts, in relation as the physico-chemical characteristics (macroscopic characteristics, test centrifugation and pH) when compared to control (time 0). The FPS test in vitro showed decrease SPF of the formulations added of plant extracts rich in phenolic compounds, except for the extract of S. adstringens. The FPS for the extracts obtained were ≤2. The percentage of the PT were (%): G. ulmifolia, 24,26±0,34; M. ilicifolia, 14,66±0,18; S. adstringens, 34,38±0,62 e T. catigua, 41,60±0,13. The values of CA (IC50) against the DPPH radical were (miug/mL): vitamin C (7,52±0,12), G. ulmifolia (8,94±0,13), M. ilicifolia (38,41±0,93), S. adstringens (7,31±0,15) e T. catigua (5,48±0,04). CA was directly proportional to the content of PT.
