ABSTRACT
Total resolution of 17 DL-amino acids after derivatization with a fluorescent chiral tagging reagent, 4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfony l)-2,1,3- benzoxadiazole [R(-)-DBD-PyNCS], was studied by reversed-phase liquid chromatography. The reaction of the reagent with amino acids proceeds effectively at 55 degrees C for 20 min in the presence of 1% TEA to produce the corresponding fluorescent diastereomers (excitation at 460 nm, emission at 550 nm). Each pair of the resulting derivatives was efficiently separated with water-acetonitrile containing 1% acetic acid as the mobile phase. Peak resolution was in the range of 0.92 (DL-Arg)-9.8 (DL-Cys). Although mutual separation of some DL-amino acids was possible using the elution solvent, simultaneous resolution of 17 DL-amino acids was difficult with a single chromatographic run, even if some gradient elutions were adopted. Therefore, both gradient and isocratic elution systems were used for total resolution of the DL-amino acids. Thus, 17 DL-amino acids were well resolved by a gradient and an isocratic elution systems. The proposed derivatization and elution methods were applied to the determination of DL-amino acids in yogurt. The results showed that some of the L-amino acids, i.e., Glu, Asp, Ser, Gly, Ala, Thr, Pro, Lys, Phe and Met, were found in the methanol extracts of yogurt. On the other hand, the D-amino acids that were identified in the extracts were D-Glu, D-Asp and D-Ala, and the mean % to each L-amino acid were 11.9% (D-Glu), 27.6% (D-Asp) and 56.7% (D-Ala), respectively.
Subject(s)
Amino Acids/analysis , Fluorescent Dyes , Isothiocyanates , Oxadiazoles , Chromatography, High Pressure Liquid , Mass Spectrometry , Stereoisomerism , Valine/analysis , Yogurt/analysisSubject(s)
Coproporphyrins/isolation & purification , Photosensitizing Agents/isolation & purification , Porphyrins/isolation & purification , Streptococcus/chemistry , Zinc , Animals , Coproporphyrins/chemistry , Coproporphyrins/pharmacology , Cromolyn Sodium/pharmacology , Hematoporphyrin Photoradiation , Histamine Antagonists/chemistry , Histamine Antagonists/isolation & purification , Histamine Antagonists/pharmacology , Magnetic Resonance Spectroscopy , Male , Mast Cells/drug effects , Mice , Mice, Inbred ICR , Photosensitizing Agents/chemistry , Photosensitizing Agents/pharmacology , Porphyrins/chemistry , Porphyrins/pharmacology , Rats , Sarcoma 180/drug therapy , Spectrophotometry, InfraredABSTRACT
Sporeamicin A, a novel antibiotic, was isolated from the culture filtrate of an actinomycete. The producing organism, strain L53-18, was taxonomically assigned as a species of the genus Saccaropolyspora. The antibiotic was extracted with chloroform and was then purified by crystallization. It was obtained as colorless prisms from ethanolic solutions. Sporeamicin A exhibited a strong UV absorption peak at 276 nm. The molecular formula of sporeamicin A was determined to be C37H63NO12.
Subject(s)
Anti-Bacterial Agents/isolation & purification , Erythromycin/analogs & derivatives , Saccharopolyspora/chemistry , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Erythromycin/chemistry , Erythromycin/isolation & purification , Erythromycin/pharmacology , Gram-Positive Bacteria/drug effects , Microbial Sensitivity Tests , Saccharopolyspora/classificationABSTRACT
Antitumor activity of Sarcodon aspratus (Berk.) S. Ito and Ganoderma lucidum (Fr.) Karst. was investigated. Methanol and aqueous extracts of these Japanese mushrooms were tested for antitumor activity against solid type of sarcoma 180 by intraperitoneal or oral administration. The aqueous extract was remarkably effective for inhibition of tumor growth, but the methanol extract was not. The fraction of molecular weight more than 10000 had a high inhibitory activity against the tumor growth, but the fraction of molecular weight less than 10000 did not. Fractionation was carried out by using an ion-exchanger, and fraction S-4 having the highest carbohydrate content had the highest antitumor activity by intraperitoneal administration.