Environmental Distractions during Unsupervised Remote Digital Cognitive Assessment.

The current demand for cognitive assessment cannot be met with traditional in-person methods, warranting the need for remote unsupervised options. However, lack of visibility into testing conditions and effort levels limit the utility of existing remote options. This retrospective study analyzed the frequency of and factors associated with environmental distractions during a brief digital assessment taken at home by 1,442 adults aged 23-84. Automated scoring algorithms flagged low data capture. Frequency of environmental distractions were manually counted on a per-frame and per-trial basis. A total of 7.4% of test administrations included distractions. Distractions were more frequent in men (41:350) than women (65:1,092) and the average age of distracted participants (51.7) was lower than undistracted participants (57.8). These results underscore the challenges associated with unsupervised cognitive assessment. Data collection methods that enable review of testing conditions are needed to confirm quality, usability, and actionability.

Adoption of rollover protective structures (ROPS) on U.S. farm tractors by state: 1993-1995, 2001, and 2004.

This research compares state-level rollover protective structure (ROPS) prevalence rates from the early and mid-1990s to those observed in the years 2001 and 2004. In addition, state-level ROPS prevalence rates are compared to state-level tractor overturn fatality rates. Tractor data for 1993-1995 and for 2001 and 2004 for all tractors and ROPS-equipped tractors in use on U.S. farms were derived from surveys conducted for NIOSH by the USDA-NASS. Changes in ROPS prevalence rates at the state level between the two time periods were assessed using a two-sample paired t-test with unequal sample sizes. Poisson regression was used to assess the association between ROPS prevalence rates and tractor overturn fatality rates at the state level. Overall, 49 of the 50 states had an observed increase in the percentage of farm tractors equipped with ROPS from 1993-1995 to 2001 and 2004. This increase was statistically significant for 34 states. Large shifts in ROPS prevalence were found within individual states and in clusters of states. These include a major increase in the southeastern U.S. and some western states. However, a core of states in the northeast (many of them in or near the Appalachian Mountains) through the upper midwest remain in the bottom quartile for ROPS prevalence. For the years 1992 through 2004, the highest fatality rates were observed in many of the same states that were identified previously as having persistently low ROPS prevalence rates. There is a clear relationship between low state-level ROPS prevalence rates and high state-specific tractor overturn fatality rates. While progress has been made in increasing the percentage of ROPS-equipped farm tractors, it is projected that ROPS prevalence rates will not reach a protective level nationally until after 2015. Regionally, the northeast and midwest will not reach protective levels of ROPS-equipped tractors until after 2020. Based on the adoption rates observed, tractor overturn rates will likely continue to be a more localized, but significant, public health issue for several states beyond the year 2020. The results of this study show the geographic areas of the U.S. where the greatest need exists, and where a greater emphasis should be placed on ROPS promotion activities. However, addressing this public health issue on a large scale will require resources and an organized commitment, which have historically been lacking.

Factors associated with the prevalence of non-ROPS tractors on farms in the U.S.

Rollover protective structures (ROPS) are an effective engineering control known to prevent tractor overturn deaths, the leading cause of occupational fatalities for farmers and farm workers in the U.S. However, the use of ROPS is known to vary greatly from farm to farm. A national sample of 11,458 farm operators from the 2004 Occupational Injury Surveillance of Production Agriculture (OISPA) survey was used to assess the association between the prevalence of ROPS and ten farm operator and farm demographic variables using logistic regression. The variable were: operator's age, operator's sex, operator's education, farm sales, full- or part-time farming, acreage, type of operation, number of hired workers, number of injuries, and region. All ten variables were found to have significant associations with the prevalence of non-ROPS tractors on farms in the univariate logistic regressions. For the multivariate model, all variables except for the sex of the farm operator remained significant. Farms with less than three adult injuries, no hired workers, less than 300 acres in size, a Midwest location, and a primary farm type of tobacco, fruit and nuts, dairy, or poultry and eggs all had adjusted odds ratios of 2 or greater. Increasing the prevalence of ROPS-equipped tractors is essential for reducing the leading cause of death on farms, tractor overturns. Economic factors play a major role in the prevalence and distribution of non-ROPS tractors on farms. The identified associations can be used to effectively target areas of the U.S. for ROPS promotion activities.

Youth living on Hispanic-operated farms: injuries and population estimates in the U.S., 2000.

A dearth of information exists in public health surveillance on the injury burden among Hispanic farm youth in the U.S. In this study, data were collected via a telephone survey with Hispanic farm operators sampled from the 1991 Census of Agriculture active list of farms. There were an estimated 307 nonfatal injuries among an estimated 21,631 youth less than 20 years old living on Hispanic-operated farms in the U.S., 2000. Males accounted for 73% of the injuries and had an injury rate of 20.2 compared to 8.2 per 1,000 for females. Injury rates were higher for work-related incidents than for nonwork, and this relationship was true for both males and females. Work and nonwork injury rates were also similar for youth < 10 and 10-15 years old, but for youth 16-19 years of age, the risk of injury for work-related incidents was higher than for nonwork. Livestock operations had a larger number of injuries and higher injury rates compared to crop operations. These data provide previously unavailable descriptive statistics for both the nonfatal injury burden and population estimate for youth on Hispanic-operated farms.

Prevalence of ROPS-equipped tractors in U.S. aquaculture.

Aquaculture involves the production of plant and animal products that are cultured in water. The principal freshwater fishes raised in the U.S. are catfish (raised mainly in ponds) and trout (raised mostly in concrete raceways), and the principal crustaceans grown are shrimp, crayfish, oysters, and clams. Tractors are used on aquaculture farms mostly in pond culture. Ponds present overturn hazards because of the slopes of levees, slippery conditions, and nighttime driving. Protection is afforded to the tractor operator when a rollover protective structure (ROPS) is attached to the tractor. The purpose of this study was to analyze and describe the prevalence of ROPS-equipped tractors on farms engaged in aquaculture in the U.S. The analysis concluded that 78% of tractors used in aquaculture were equipped with a ROPS, in contrast with the prevalence of ROPS at 49% for all of agriculture. Moreover, 91% of the tractors in the South used for aquaculture were equipped with a ROPS. The national sample for aquaculture included 75 farms and 137 tractors, which is small, but nonetheless, several hypotheses can be generated as a result of this descriptive study.

Nonfatal injuries to household youth on racial minority-operated farms in the U.S., 2000.

The National Institute for Occupational Safety and Health (NIOSH) estimated that 32,808 nonfatal injuries occurred to youth less than 20 years of age on U.S. farms during 1998. These data, however, do not allow for the identification of minority farm operators. The Minority Farm Operator Childhood Agricultural Injury Survey (M-CAIS) was conducted to provide an overview of the number of youth on minority-operated farms and their associated farm-related injuries during 2000. M-CAIS was conducted by the USDA for NIOSH through a telephone survey of 49,270 minority-operated farms identified in the 1997 Census of Agriculture. These minority-operated farms included four racial categories (black, Asian, Native American, and other) and operators of Hispanic ethnicity. This study included only racial minority-operated farms for analysis, white Hispanic farms were excluded. In 2000, there were an estimated 28,577 youth living on U.S. farms operated by racial minorities. In that year, these youth sustained an estimated 348 nonfatal injuries. Males accounted for 245 (70%) of the injuries to household youth. The majority of all injuries to household youth (247, 71%) occurred on livestock operations. Native American household youth accounted for both the largest number of injuries (177) and the highest rate of injury (24.0/1,000 household youth) on these farms. M-CAIS data indicated significant variation in injury rates among specific racial categories. Results of the M-CAIS suggest the need for prevention strategies to address issues found within these specific sub-populations of the agricultural community.

On-farm falls among youth less than 20 years old in the U.S.

This article examines the magnitude and characteristics of fall-related injuries on U.S. farms for youth less than 20 years old for work and non-work exposures at a national level. To examine the problem, data from the Childhood Agricultural Injury Survey (CAIS) and Census of Fatal Occupational Injuries (CFOI) were used. Findings indicate that falls are an important contributor to on-farm injuries, with youth appearing to be at considerable risk. Thus, a reduction of the exposure of youth to fall-related hazards on farms is needed. Strategies such as providing safe play areas for young children and continuing efforts to prevent extra riders on farm equipment will help in reducing these hazardous fall exposures.

Characterization and inheritance of the Anthocyanin fruit (Aft) tomato.

Tomato (Lycopersicon esculentum) accession LA1996 with the Anthocyanin fruit (Aft) gene has dark green foliage, elevated anthocyanin expression in the hypocotyls of seedlings, and anthocyanin in the skin and outer pericarp tissues of the fruit. Interest in the health benefits and antioxidant capacity of anthocyanins led to this study of the genetic potential for increased levels of this important class of phytonutrients in tomato fruit. In order to conform to tomato gene nomenclature rules, we propose changing the symbol Af for Anthocyanin fruit to Aft. Segregation ratios of anthocyanin expression in F(2) and BC(1) populations of a cross between the processing tomato UC82B and LA1996 were consistent with a single dominant gene hypothesis. Anthocyanin expression was reduced in backcross populations compared to F(2 )populations. Anthocyanin concentration, as measured by the pH differential method, of pigment-rich pericarp and skin tissues from LA1996 was estimated to be 20.6 mg/100 g and 66.5 mg/100 g, respectively. Anthocyanidin composition was characterized by high-performance liquid chromatography (HPLC). Fruit of accession LA1996 contained predominantly petunidin, followed by malvidin and delphinidinin. Lycopene, beta-carotene, phytoene, and phytofluene levels were similar to those of normal tomatoes and lower than those found in high pigment tomatoes.

Genetic Characterization of Differential Reactions Among Host Group 3 Common Bean Cultivars to NL-3 K Strain of Bean common mosaic necrosis virus.

ABSTRACT A previously unrecognized recessive resistance gene (or allele) was identified in three host group (HG) 3 common bean (Phaseolus vulgaris) cvs. Olathe, Victor, and UI 37, based on genetic analysis of plants from five populations screened with the NL-3 K strain of Bean common mosaic necrosis virus (BCMNV). The gene (or allele) was associated with resistance to leaf stunting and deformity and reduction in plant height. The gene (or allele) provides similar, but slightly better resistance than the bc-1(2) gene that is characteristic of HG 3 cultivars. Traditional HG 3 cultivars like Redlands Greenleaf B with bc-1(2) are susceptible to NL-3 K, whereas this newly identified gene (or allele) conditions resistance to NL-3 K. Other slight variations in disease reaction pattern to a wide array of bean common mosaic (BCM)-inducing strains were noted among HG 3 differentials, indicating that additional resistance to BCM exists in common bean that remains to be exploited. To gauge the full breeding value of this newly identified gene (or allele), allelism tests with existing genes, namely bc-1(2), and further characterization of responses to all Bean common mosaic virus (BCMV) and BCMNV strains need to be conducted. Meanwhile, breeders should consider introgressing this more effective gene (or allele) into susceptible cultivars while plant pathologists continue to decipher the genetic variability present among HG 3 differential cultivars.

A Quantitative Method to Screen Common Bean Plants for Resistance to Bean common mosaic necrosis virus.

ABSTRACT A quantitative method to screen common bean (Phaseolus vulgaris) plants for resistance to Bean common mosaic necrosis virus (BCMNV) is described. Four parameters were assessed in developing the quantitative method: symptoms associated with systemic virus movement, plant vigor, virus titer, and plant dry weight. Based on these parameters, two rating systems (V and VV rating) were established. Plants from 21 recombinant inbred lines (RILs) from a Sierra (susceptible) x Olathe (partially resistant) cross inoculated with the BCMNV-NL-3 K strain were used to evaluate this quantitative approach. In all, 11 RILs exhibited very susceptible reactions and 10 RILs expressed partially resistant reactions, thus fitting a 1:1 susceptible/partially resistant ratio (chi(2) = 0.048, P = 0.827) and suggesting that the response is mediated by a single gene. Using the classical qualitative approach based only on symptom expression, the RILs were difficult to separate into phenotypic groups because of a continuum of responses. By plotting mean percent reduction in either V (based on visual symptoms) or VV (based on visual symptoms and vigor) rating versus enzyme-linked immunosorbent assay (ELISA) absorbance values, RILs could be separated clearly into different phenotypic groups. The utility of this quantitative approach also was evaluated on plants from 12 cultivars or pure lines inoculated with one of three strains of BCMNV. Using the mean VV rating and ELISA absorbance values, significant differences were established not only in cultivar and pure line comparisons but also in virus strain comparisons. This quantitative system should be particularly useful for the evaluation of the independent action of bc genes, the discovery of new genes associated with partial resistance, and assessing virulence of virus strains.

Traumatic injuries in agriculture.

The National Coalition for Agricultural Safety and Health (NCASH) in 1988 addressed issues in agriculture and noted "a sense of urgency... arose from the recognition of the unabating epidemic of traumatic death and injury in American farming . . ." This article provides an update to the NCASH conference on traumatic injuries in agriculture, a history on how the facts and figures were arrived at for the NCASH conference, and a current report on the status of traumatic injuries in agriculture in the U.S. Fatal and nonfatal injuries are addressed along with national and regional surveillance systems. The Census of Fatal Occupational Injuries (CFOI) was used for reporting national agricultural production fatal injuries from 1992-1998 (25.8 deaths per 100,000 workers), the Traumatic Injury Surveillance of Farmers (TISF) 1993-1995 was used to report nonfatal injuries occurring nationally (7.5/100 workers), and Regional Rural Injury Studies I and II (RRIS-I and RRIS-II) were used to illustrate a regional approach along with in-depth, specific analyses. Fatality rates, which showed some decline in the 1980s, were fairly constant during the 1990s. Changes in nonfatal injury rates for this sector could not be assessed due to a lack of benchmark data. The main concerns identified in the 1989 NCASH report continue today: tractors are the leading cause of farm-related death due mostly to overturns; older farmers continue to be at the highest risk for farm fatalities; and traumatic injuries continue to be a major concern for youth living or working on U.S. farms. Fatal and nonfatal traumatic injuries associated with agricultural production are a major public health problem that needs to be addressed through comprehensive approaches that include further delineation of the problem, particularly in children and older adults, and identification of specific risk factors through analytic efforts. Continued development of relevant surveillance systems and implementation of appropriate interventions are the primary challenges for the current decade.

Fatal on-farm injuries among youth 16 to 19 years of age: 1982-1994.

Data from the Vital Statistics Mortality (VSM) public use file and the National Traumatic Occupational Fatalities (NTOF) surveillance systems were used to describe fatal injuries among youth 16-19 years of age in the United States that occurred on farms for the years 1982 through 1994. The VSM captures all deaths in the United States, while the NTOF only captures occupational injury deaths. There were 550 total on-farm fatalities to youth 16-19 years of age in the VSM, and 221 occupational on-farm deaths from the NTOF for the same age group. These numbers suggest that 40% of the on-farm deaths were occupational. It was found that the proportions of deaths attributable to work increased with age. Fatality rates for on-farm nonoccupational deaths decreased slightly during the time period (from 8.4 deaths/100,000 for 1982-1985 to 6.8 deaths/100,000 for 1991-1994), while on-farm occupational fatality rates dropped dramatically (12.0 deaths/100,000 for 1982-1985 down to 4.9 deaths/100,000 for 1991-1994). The leading causes of death for on-farm occupational fatalities were machinery (54%) and electrical current (20%). The most common causes of on-farm fatalities that were nonoccupational were drowning (38.9%) and firearms (28.6%). For the years 1991 through 1994, drowning and firearms accounted for approximately the same number of on-farm deaths as machinery. Nonoccupational risks are a concern for youth 16-19 years of age on the farm.

Crg, a gene required for Ur-3-mediated rust resistance in common bean, maps to a resistance gene analog cluster.

Race-specific resistance to the bean rust pathogen (Uromyces appendiculatus) is provided by a number of loci in common bean (Phaseolus vulgaris). The Ur-3 locus controls hypersensitive resistance (HR) to 44 of the 89 races curated in the United States. To better understand resistance mediated by this locus, we developed new genetic material for analysis. We developed a population of mutagenized seed of cv. Sierra (genotype = Ur-3 ur-4 ur-6) that was screened with a bean rust race that is normally incompatible (HR response) on Ur-3 genotypes. We discovered two mutants of common bean, crg and ur3-delta3, in which uredinia formed on leaves (a compatible interaction) following infection. The F1 generation from a cross of these two mutants expressed the HR response, and the F2 generation segregated in a ratio of 9:7 (HR/uredinia formation). Therefore, the two genes are unlinked. Further genetic analysis determined that the mutation in ur3-delta3 was in the Ur-3 locus, and the mutation in crg was in a newly discovered gene given the symbol Crg (Complements resistance gene). Each mutation was inherited in a recessive manner. Unlike ur3-delta3, crg expressed reduced compatibility to bean rust races 49 and 47 that are normally fully compatible on genotypes, such as Sierra, that are homozygous recessive at the Ur-4 and Ur-6 loci. This suggests a gene mutated in crg is normally a positive compatibility factor for the bean-bean rust interaction. Polymerase chain reaction analysis of crg with primers to common bean resistance gene analogs (RGA) that contain a nucleotide-binding site sequence similar to those found in a number of plant disease resistance genes revealed that crg is missing the SB1 RGA, but not the linked SB3 and SB5 RGAs. Genetic analyses revealed that Crg cosegregates with the SB1 RGA. These results demonstrate that Crg is located near a RGA cluster in the common bean genome.

Fatal harmful substances or environmental exposures in agriculture, 1992 to 1996.

Data from the Census of Fatal Occupational Injuries surveillance system from 1992 through 1996 were analyzed to allow a better understanding of exposures to harmful substances or environments that resulted in agricultural work fatalities. There were 357 fatalities as a result of these exposures in the agriculture production and agriculture services sectors, representing 10% of all work-related deaths that occurred in these industry sectors during this period. Contact with electric current represented 52.9% of these fatalities. Agricultural services reported 87 electrocutions, 50 of which occurred among tree trimmers. The events most likely to result in fatalities were contact with overhead power lines (26.3%) and drowning (17.1%). The overall fatality rate was 2.1 deaths per 100,000 workers. The development of appropriate hazard-awareness training for workers, such as that for electrical and drowning-related hazards, may help prevent future deaths in these industry sectors.

First Report of Red Clover Vein Mosaic Carlavirus Naturally Infecting Lentil.

Lentil (Lens culinaris Medik.) is an important legume crop grown in the dryland Pacific Northwest areas of eastern Washington and Oregon, and northern Idaho. Lentil is highly susceptible to pea enation mosaic enamovirus (PEMV) and bean leafroll luteovirus (BLRV), and infection may result in severe yield losses. Recently, lentil was also found to be infected experimentally with red clover vein mosaic carlavirus (RCVMV) (1). The virus is most commonly transmitted in the Pacific Northwest by the pea aphid (Acyrthosiphon pisum Harris) in a nonpersistent manner. In 1997, cv. Brewer lentil bait plants were planted at the Vegetable Research Farm at Oregon State University to monitor incidence of PEMV and BLRV. Many of the plants developed symptoms typical of PEMV. However, other plants exhibited severe stunting, proliferation of axillary branches, and general chlorosis or death. Bait plants were harvested in August, and 204 random samples were tested for PEMV, RCVMV, BLRV, alfalfa mosaic alfamovirus (AMV), and pea streak carlavirus (PeSV) by standard enzyme-linked immunosorbent assay (ELISA) protocols. Antiserum for RCVMV was made in the Prosser lab against an isolate from chickpea collected in Washington State (1). RCVMV was detected in 76 (34%) of the 204 samples. PEMV, AMV, BLRV, and PeSV were detected in 197 (89.5%), 23 (11.3%), 2 (0.9%), and 0 (0%) of samples, respectively. Results showed that 75/76 of the samples positive for RCVMV were also coinfected with PEMV. Plants infected with RCVMV in the greenhouse also produced mild systemic mosaic symptoms in selected hosts inoculated mechanically, including pea (Pisum sativum L.), chickpea (Cicer arietinum L.), faba bean (Vicia faba L.), and lentil. Lentil and chickpea also showed moderate to severe stunting. Chlorotic local lesions were formed on Chenopodium amaranticolor Coste & Reyn. and C. quinoa Willd. Oligonucleotide primers were designed with sequence data obtained from the Washington isolate of RCVMV (1), and identification of the virus was verified in pea and lentil by polymerase chain reaction (PCR). Primer design of RCV34V and RCV653C targeted a 619-bp fragment located in the viral coat protein gene. Plants testing positive by ELISA yielded PCR products of the expected size when visualized on agarose gels. This is the first report of natural infection of lentil by RCVMV. Reference: (1) R. C. Larsen et al. Phytopathology 87:S56, 1997.

Working lifetime risk of occupational fatal injury.

Estimates of risk accumulated over a working lifetime are used to assess the significance of many workplace health hazards. Utilizing data from the National Traumatic Occupational Fatalities (NTOF) surveillance system, estimates of the risk of work-related fatal injuries are provided for the 50 industries and the 50 occupations having the highest risks. Cause-specific risk estimates are provided for the six occupations at the greatest risk of occupational fatal injuries. Results suggest that the risks of certain work-related fatal injuries in some occupations (e.g., loggers being struck by falling objects) are of the same magnitude as risks previously identified for specific occupational illness exposures (e.g., lung cancer among uranium miners exposed to ionizing radiation). Assuming a 45-year working lifetime, cause-specific fatal injury risks reported in this paper range from a predetermined minimum of 1 death per 1,000 lifetime workers to 36.4 deaths per 1,000 lifetime workers. These results suggest that risk assessment for traumatic causes of death should be considered equally with risk assessments for health exposures, such as potential carcinogens.

Work-related fatalities in the agricultural production and services sectors, 1980-1989.

A total of 6,727 workers died of work-related injuries in the agricultural production and agricultural services sectors between 1980 and 1989, as established by data from the National Institute for Occupational Safety and Health (NIOSH) National Traumatic Occupational Fatalities (NTOF) surveillance system. The agricultural production sector accounted for the higher fatality rate (22.9 deaths per 100,000 workers), due largely to deaths caused by machinery and motor vehicles. The leading cause of death in the agricultural services sector was being struck by falling objects, primarily trees. Fatality rates were highest in the East South Central United States and lowest in the New England states. Blacks had the highest fatality rate (26.4 deaths per 100,000 workers) while workers other than white or black had the lowest rate (18.9 per 100,000 workers). Males were at higher risk of death than females, with the 65 years of age and older male group having the highest rate (60.5 deaths per 100,000 workers). Males 16-24 years of age exhibited the largest decrease in their average annual fatality rate during the 10-year period, down to 7.2 from 20.6 deaths per 100,000 workers. Possible reasons for this decrease are suggested.