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Vet Microbiol ; 146(1-2): 155-60, 2010 Nov 20.
Article in English | MEDLINE | ID: mdl-20570062

ABSTRACT

Here we report the isolation of Newcastle disease virus (NDV) from cloacal swabs obtained from penguins in the South Atlantic Antarctic region (62°08S, 58°25W). Samples of 100 penguins from King George Island were tested by real-time PCR, of which 2 (2%) were positive for NDV. The positive samples were isolated in embryonated chicken eggs and their matrix and fusion proteins genes were partially sequenced. This was complemented by the serological study performed on the blood of the same specimens, which resulted in a 33.3% rate of positivity.


Subject(s)
Newcastle Disease/epidemiology , Newcastle disease virus/isolation & purification , Spheniscidae/virology , Animals , Animals, Wild/virology , Antarctic Regions/epidemiology , Base Sequence , Molecular Sequence Data , Newcastle Disease/virology , Phylogeny , Reverse Transcriptase Polymerase Chain Reaction/veterinary
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