ABSTRACT
The synthesis of polymer-encapsulated metal nanoparticles is a growing field of area due to their long-term uses in the development of new technologies. The present study describes the synthesis of chitosan/silver nanocomposite using kaempferol for anticancer and bactericidal activity. The formation of Kf-CS/Ag nanocomposite was confirmed by the development of a brown color and UV-absorbance around 438 nm. The IR study was utilized to determine the existence of Kf and CS in the synthesized nanocomposite. TEM analysis demonstrated that the synthesized nanocomposite have a predominantly uniform spherical shape and size ranges 7-10 nm. EDX spectrum showed the existence of Ag, C, and N elements in the nanocomposite material. Further, Kf-CS/Ag nanocomposite exhibited potential in vitro inhibitory property against triple-negative breast cancer (TNBC) cells and their IC50 values was found to be 53 µg/mL. Moreover, fluorescent assays such as DAPI and AO/EtBr confirmed the apoptosis induction ability of Kf-CS/Ag nanocomposite in MDA-MB-231 cells. The synthesized Kf-CS/Ag nanocomposite showed significant and dose-depended antibacterial property against S. aureus and P. aeruginosa. Thus, the obtained findings demonstrated that the synthesized nanocomposite can be potentially used to improve human health as biocidal nanocomposite in biomedical sectors.
Subject(s)
Chitosan , Metal Nanoparticles , Nanocomposites , Triple Negative Breast Neoplasms , Humans , Staphylococcus aureus , Silver , Kaempferols , Anti-Bacterial Agents/pharmacology , Microbial Sensitivity TestsABSTRACT
The coloured effluents produced from different industries, such as textile, plastics, printing, cosmetics, leather and paper, are extremely toxic and a tremendous threat to the aquatic organisms and human beings. The removal of coloured dye pollutants from the aqueous environment is a great challenge and a pressing task. The growing demand for low-cost and efficient treatment approaches has given rise to alternative and eco-friendly methods, such as biodegradation and microbial remediation. This work summarizes the overview and current research on the remediation of dye pollutants from the aqueous environment by microbial bio-sorbents, such as bacteria, fungi, algae, and yeast. In addition, dye degradation capabilities of microbial enzymes have been highlighted and discussed. Further, the influence of various experimental parameters, such as temperature, pH, and concentrations of nutrients, and dye, has been summarized. The proposed mechanism for dye removal by microorganisms is also discussed. The object of this review is to provide a state-of-the-art of microbial remediation technologies in eliminating dye pollutants from water resources.