ABSTRACT
PURPOSE: To assess oxidative stress and the profile of fatty acids incorporated into the hepatic tissue of animals refed with high-fat (HF) diets after acute food restriction. METHODS: Fifty male Wistar rats were divided into five groups and fasting for 48 hours. One group was sacrificed without refeeding (NR), a control group (C) was refed with the standard AIN-93 diet and the remaining groups with HF diets respectively consisting of hydrogenated vegetable oil (PHVO), trans-free (TF) margarine and trans-free margarine enriched with ω-3 and ω-6 (O). After this period the animals were sacrificed for malondialdehyde (MDA), catalase and hepatic fatty acid determination. RESULTS: The groups refed with HF diets showed elevation of MDA levels compared to the C group (p<0.001 for GVH and p<0.01 for TF and O). Hepatic catalase activity was higher in the TF and O groups compared to group C (p<0.05 for both). The amount of saturated fatty acids was lower in the PHVO and O groups compared to the remaining ones (p<0.001). CONCLUSION: The consumption of high-fat diets after prolonged fasting favors oxidative imbalance in hepatic tissue.
Subject(s)
Diet, High-Fat , Fasting/physiology , Fatty Acids/analysis , Liver/metabolism , Oxidative Stress/physiology , Animals , Catalase/physiology , Fatty Liver/metabolism , Liver/chemistry , Male , Malondialdehyde/analysis , Models, Animal , Random Allocation , Rats , Rats, Wistar , Reference Values , Time FactorsABSTRACT
To assess oxidative stress and the profile of fatty acids incorporated into the hepatic tissue of animals refed with high-fat (HF) diets after acute food restriction. METHODS: Fifty male Wistar rats were divided into five groups and fasting for 48 hours. One group was sacrificed without refeeding (NR), a control group (C) was refed with the standard AIN-93 diet and the remaining groups with HF diets respectively consisting of hydrogenated vegetable oil (PHVO), trans-free (TF) margarine and trans-free margarine enriched with ω-3 and ω-6 (O). After this period the animals were sacrificed for malondialdehyde (MDA), catalase and hepatic fatty acid determination. RESULTS: The groups refed with HF diets showed elevation of MDA levels compared to the C group (p<0.001 for GVH and p<0.01 for TF and O). Hepatic catalase activity was higher in the TF and O groups compared to group C (p<0.05 for both). The amount of saturated fatty acids was lower in the PHVO and O groups compared to the remaining ones (p<0.001). CONCLUSION: The consumption of high-fat diets after prolonged fasting favors oxidative imbalance in hepatic tissue.(AU)
Subject(s)
Animals , Rats , Diet , Oxidative Stress , Fats/analysis , Rats/classificationABSTRACT
To assess oxidative stress and the profile of fatty acids incorporated into the hepatic tissue of animals refed with high-fat (HF) diets after acute food restriction. METHODS: Fifty male Wistar rats were divided into five groups and fasting for 48 hours. One group was sacrificed without refeeding (NR), a control group (C) was refed with the standard AIN-93 diet and the remaining groups with HF diets respectively consisting of hydrogenated vegetable oil (PHVO), trans-free (TF) margarine and trans-free margarine enriched with ω-3 and ω-6 (O). After this period the animals were sacrificed for malondialdehyde (MDA), catalase and hepatic fatty acid determination. RESULTS: The groups refed with HF diets showed elevation of MDA levels compared to the C group (p<0.001 for GVH and p<0.01 for TF and O). Hepatic catalase activity was higher in the TF and O groups compared to group C (p<0.05 for both). The amount of saturated fatty acids was lower in the PHVO and O groups compared to the remaining ones (p<0.001). CONCLUSION: The consumption of high-fat diets after prolonged fasting favors oxidative imbalance in hepatic tissue.
Subject(s)
Animals , Rats , Diet , Fats/analysis , Oxidative Stress , Rats/classificationABSTRACT
O enfermeiro nas instituições hospitalares é visto como fonte de influência e liderança, principalmente por sua competência na área de gestão e atuação em tempo integral. Cabe a ele conciliar os objetivos individuais dos integrantes da equipe aos objetivos da organização, com foco em assistência de qualidade. Assim, é fundamental que detenha conhecimentos sobre liderança e habilidades interpessoais para promover mudanças. Essa pesquisa utilizou a Metodologia da Educação Permanente (EP) proposta pelo Ministério da Saúde (MS) e foi realizada com enfermeiros de um hospital do interior do Estado de São Paulo. As considerações desta ação educativa, oriunda de profundas reflexões, mostram que o exercício da liderança na enfermagem deve ser uma escolha pessoal, pois o mesmo faz parte de um contínuo processo de auto desenvolvimento, provocado por mudanças interna de paradigmas.