Multivariate Statistical Analysis for the Classification of Sausages Based on Physicochemical Attributes, Using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) and Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).

Sausage is a convenient food that is widely consumed in the world and in Vietnam. Due to the rapid development of this product, the authenticity of many famous brands has faded by the rise of adulteration. Therefore, in this study, principal component analysis (PCA) was combined with chemical analysis to identify 6 sausage brands. Sausage samples were dried and then ground to a fine powder for both instrumental analyses of attenuated total reflectance-Fourier transform infrared (ATR-FTIR) and inductively coupled plasma-mass spectrometry (ICP-MS). Dried measurements of ATR-FTIR was performed directly on the ZnSe crystal, while elemental data were obtained through microwave digestion before the ICP-MS analysis. Principal component analysis (PCA) within the framework software of XLSTAT and STATISTICA 12 was performed on the spectroscopy and elemental dataset of sausage samples. PCA visualized the distinction of 6 sausage brands on both datasets of ATR-FTIR and ICP-MS. The classification on the spectroscopy profile showed that although more than 90% variation of the dataset was explained on the first two PCs, the difference between several brands was not detected as the distribution of data overlapped with one another. The PCA observation of the elemental composition on PC1 and PC3 has separated the sausage brands into 6 distinctive groups. Besides, several key elements contributed to the brands' identification have been detected, and the most distinctive elements are Na, K, Ca, and Ba. PCA visualization showed the feasibility of the classification of sausage samples from different brands when combined with the results of FT-IR and ICP-MS methods. The experiment was able to differentiate the sausages from the 5 brands using multivariate statistics.

Classification of Vietnamese Cashew Nut (Anacardium occidentale L.) Products Using Statistical Algorithms Based on ICP/MS Data: A Study of Food Categorization.

Fingerprinting techniques, which utilize the unique chemical and physical properties of food samples, have emerged as a promising approach for food authentication and traceability. Recent studies have demonstrated significant advancements in food authentication through the use of fingerprinting methods, such as multivariate statistical analysis techniques applied to trace elements and isotope ratios. However, further research is required to optimize these methods and ensure their validity and reliability in real-world applications. In this study, the inductively coupled plasma mass spectrometry (ICP-MS) analytical method was employed to determine the content of 21 elements in 300 cashew nut (Anacardium occidentale L.) samples from 5 brands. Multivariate statistical methods, such as principal components analysis (PCA), were employed to analyze the data obtained and establish the provenance of the cashew nuts. While cashew nuts are widely marketed in many countries, no universal method has been utilized to differentiate the origin of these nuts. Our study represents the initial step in identifying the geographical origin of commercial cashew nuts marketed in Vietnam. The analysis showed significant differences in the means of 21 of the 40 analyzed elements among the cashew nut samples from the 5 brands, including 7Li, 11B, 24Mg, 27Al, 44Ca, 48Ti, 51V, 52Cr, 55Mn, 57Fe, 60Ni, 63Cu, 66Zn, 93Nb, 98Mo, 111Cd, 115In, 121Sb, 138Ba, 208Pb, and 209Bi. The PCA analysis indicated that the cashew nut samples can be accurately classified according to their original locations. This research serves as a prerequisite for future studies involving the combination of elemental composition analysis with statistical classification methods for the accurate establishment of cashew nut provenance, which involves the identification of key markers for the original discrimination of cashew nuts.

Towards a Standardized Approach for the Geographical Traceability of Plant Foods Using Inductively Coupled Plasma Mass Spectrometry (ICP-MS) and Principal Component Analysis (PCA).

This paper presents a systematic literature review focused on the use of inductively coupled plasma mass spectrometry (ICP-MS) combined with PCA, a multivariate technique, for determining the geographical origin of plant foods. Recent studies selected and applied the ICP-MS analytical method and PCA in plant food geographical traceability. The collected results from many previous studies indicate that ICP-MS with PCA is a useful tool and is widely used for authenticating and certifying the geographic origin of plant food. The review encourages scientists and managers to discuss the possibility of introducing an international standard for plant food traceability using ICP-MS combined with PCA. The use of a standard method will reduce the time and cost of analysis and improve the efficiency of trade and circulation of goods. Furthermore, the main steps needed to establish the standard for this traceability method are reported, including the development of guidelines and quality control measures, which play a pivotal role in providing authentic product information through each stage of production, processing, and distribution for consumers and authority agencies. This might be the basis for establishing the standards for examination and controlling the quality of foods in the markets, ensuring safety for consumers.

Geographical origin traceability of Sengcu rice using elemental markers and multivariate analysis.

Multi-element analysis combined with chemometric method has been used to investigate the distinguish between Sengcu rice and other types of rice origins in Vietnam. In Sengcu rice, As, Ba Sr, Pb, Ca, Se were confirmed as the key elements for geographical traceability among three fields of Lao Cai, whereas Al, Ca, Fe, Mg, Ag, As were major factors to distinguish between Sengcu and other types of rice. Based on linear discriminant analysis and partial least squares-discriminant analysis model, overall correct identification rates distinguishing between Sengcu and other types of rice were approximately 100% in both training and validation test. Moreover, to distinguish geographical origin of Sengcu rice samples, these rates vary from 80% to 99%. These results suggest the presence of food adulteration illustrated in the latter.

Reproducibility of apparent diffusion coefficient measurement in normal prostate peripheral zone at 1.5T MRI.

PURPOSE: The purpose of this study was to quantify the influence of factors of variability on apparent diffusion coefficient (ADC) estimation in the normal prostate peripheral zone (PZ). MATERIALS AND METHODS: Fifty healthy volunteers underwent in 2017 (n = 17) or 2020 (n = 33) two-point (0, 800 s/mm²) prostate diffusion-weighted imaging in the morning on 1.5 T scanners A and B from different manufacturers. Additional five-point (50, 150, 300, 500, 800 s/mm²) acquisitions were performed on scanner B in the morning and evening. ADC was measured in PZ at midgland using ADC maps reconstructed with various b-value combinations. ADC distributions from 2017 and 2020 were compared using Wilcoxon rank sum test. ADC obtained in the same volunteers were compared using Bland Altman methodology. The 95% confidence interval upper limit of the repeatability/reproducibility coefficient defined the lowest detectable ADC difference. RESULTS: Forty-nine participants with a mean age of 24.6 ± 3.8 [SD] years (range: 21-37 years) were finally included. ADC distributions from 2017 and 2020 were not significantly different and were combined. Despite high individual variability, there was no significant bias (10 × 10-6 mm²/s, P = 0.58) between ADC measurements made on both scanners. On scanner B, differences in lowest b-values chosen within the 0-500 s/mm² range for two-point ADC computation induced significant biases (56-109 × 10-6 mm²/s, P < 0.0001). ADC was significantly lower in the morning (bias: 33 × 10-6 mm²/s, P = 0.006). The number of b-values had little influence on ADC values. The lowest detectable ADC difference varied from 85 × 10-6 to 311 × 10-6 mm²/s across scanners, b-value combinations and periods of the day. CONCLUSIONS: The MRI scanner, the lowest b-value used and the period of the day induce substantial variability in ADC computation.

Effective mitigation of single-component and mixed textile dyes from aqueous media using recyclable graphene-based nanocomposite.

The present study reported the synthesis and utilization of a graphene-based hybrid nanocomposite (MnFe2O4/G) to mitigate several synthetic dyes, including methylene blue, malachite green, crystal violet, and Rhodamine B. This adsorbent was structurally analyzed by several physicochemical techniques such as X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, Raman spectroscopy, N2 adsorption-desorption isotherm measurement, point of zero charge, and Boehm titrations. BET surface area of MnFe2O4/G was measured at 382.98 m2/g, which was substantially higher than that of MnFe2O4. MnFe2O4/G possessed diverse surface chemistry properties with the presence of many functional groups such as carboxylic acid, phenolic, lactone, and basic groups. MnFe2O4/G was used to remove synthetic dyes in the aqueous media. The effect of many factors, e.g., concentration (5-50 mg/L), pH (4-10), dose (5-20 mg), and temperature (25-45 °C) on adsorption performance of MnFe2O4/G was conducted. Kinetic, isotherm, intraparticle, and thermodynamic models were adopted for investigating adsorption phenomenon of dyes on MnFe2O4/G. The maximum adsorption capacity of dyes over MnFe2O4/G was found as Rhodamine B (67.8 mg/g) < crystal violet (81.3 mg/g) < methylene blue (137.7 mg/g) < malachite green (394.5 mg/g). Some tests were performed to remove mixed dyes, and mixed dyes in the presence of antibiotics with total efficiencies of 65.8-87.9% after 120 min. Moreover, the major role of π-π stacking interaction was clarified to gain insight into the adsorption mechanism. MnFe2O4/G could recycle up to 4 cycles, which may be beneficial for further practical water treatment.

Rapid Identification of Geographical Origin of Commercial Soybean Marketed in Vietnam by ICP-MS.

Inductively coupled plasma mass spectrometry (ICP-MS) analytical method was used to determine the content of 40 elements in 38 soybean samples (Glycine Max) from 4 countries. Multivariate statistical methods, such as principal components analysis (PCA), were performed to analyze the obtained data to establish the provenance of the soybeans. Although soybean is widely marketed in many countries, no universal method is used to discriminate the origin of these cereals. Our study introduced the initial step to the identification of the geographical origin of commercial soybean marketed in Vietnam. The analysis pointed out that there are significant differences in the mean of 33 of the 40 analyzed elements among 4 countries' soybean samples, namely, 11B, 27Al, 44Ca, 45Sc, 47Ti, 55Mn, 56Fe, 59Co, 60Ni, 63Cu, 66Zn, 69Ga, 75As, 78Se, 85Rb, 88Sr, 89Y, 90Zr, 93Nb, 95Mo, 103Rh, 137Ba, 163Dy, 165Ho, 175Lu, 178Hf, 181Ta, 182W, 185Re, 197Au, 202Hg, 205Tl, and 208Pb. The PCA analysis showed that the soybean samples can be classified correctly according to their original locations. This research can be used as a prerequisite for future studies of using the combination of elemental composition analysis with statistical classification methods for an accurate provenance establishment of soybean, which determined a variation of key markers for the original discrimination of soybean.

Auxin production of the filamentous cyanobacterial Planktothricoides strain isolated from a polluted river in Vietnam.

Cyanobacteria are photosynthetic microorganisms with widespread diversity and extensive global distribution. They produce a wide variety of bioactive substances (e.g., lipopeptides, fatty acids, toxins, carotenoids, vitamins and plant growth regulators) that are released into culture media. In this study, the capability of a cyanobacterial strain of Planktothricoides raciborskii to produce intra- and extracellular auxins was investigated. The filamentous cyanobacterial P. raciborskii strain was isolated from a river in Vietnam, and it was cultivated in the laboratory under the optimum conditions of the BG11 culture medium and a pH of 7.0. The auxins were identified and quantified by the Salkowski colorimetric method and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS). Colorimetric analysis revealed that P. raciborskii produces extracellular indole-3-acetic acid (IAA) in the absence and presence of l-tryptophan. The maximum extracellular IAA concentration of the culture reached 118 ± 2 µg mL-1, which was supplemented with 900 µg mL-1 of l-tryptophan. HPLC-MS analysis revealed that the isolated cyanobacteria accumulate other plant-growth-promoting hormones besides IAA, such as indole-3-carboxylic acid (ICA), indole-3 butyric acid (IBA) and indole propionic acid (IPA). This is the first report on the production of auxins in an isolated strain of cyanobacteria Planktothricoides from a polluted river. The capability of producing auxins makes the P. raciborskii strain an appropriate candidate for the formulation of a biofertilizer.

Synthesis, Characterization, and In Vitro Cytotoxicity of Unsymmetrical Tetradentate Schiff Base Cu(II) and Fe(III) Complexes.

Unsymmetrical tetradentate Schiff base Fe(III) and Cu(II) complexes were prepared by the coordination of some unsymmetrical tetradentate Schiff base ligands with CuCl2·2H2O or FeCl3·6H2O. The obtained complexes were characterized by ESI-MS, IR, and UV-Vis. The spectroscopic data with typical signals are in agreement with the suggested molecular formulae of the complexes. Their cyclic voltammetric studies in acetonitrile solutions showed that the Cu(II)/Cu(I) and Fe(III)/Fe(II) reduction processes are at (-)1.882-(-) 1.782 V and at (-) 1.317-(-) 1.164 V, respectively. The in vitro cytotoxicity of obtained complexes was screened for KB and Hep-G2 human cancer cell lines. The results showed that almost unsymmetrical tetradentate Schiff base complexes have good cytotoxicity. The synthetic complexes bearing the unsymmetrical tetradentate Schiff base ligands with different substituted groups in the salicyl ring indicate different cytotoxicity. The obtained Fe(III) complexes are more cytotoxic than Cu(II) complexes and relative unsymmetric Schiff base ligands.

Multielement Analysis of Pakchoi (Brassica rapa L. ssp. chinensis) by ICP-MS and Their Classification according to Different Small Geographical Origins.

Statistical interpretation of the concentrations of 42 elements, determined using solution-based inductively coupled plasma mass spectrometry (ICP-MS) analysis and multivariate statistical methods, such as principal components analysis (PCA), was used to establish the provenance of pakchoi (Brassica rapa L. ssp. chinensis) from 6 areas in Ha Noi, Vietnam. Although pakchoi is widely cultivated and manufactured, no universal method is used to discriminate the origin of this vegetable. Our study introduced for the first time a method to classify pakchoi in small geographical areas. 42 metallic elements of pakchoi were detected by ICP-MS, which were further analyzed using multivariate statistical analysis to perform clusters based on the geographical locations. Eleven elements, i.e., 28Si; 56Fe; 59Co; 63Cu; 69Ga; 75As; 85Rb; 93Nb; 107Ag; 118Sn, and 137Ba, were identified as discriminators to distinguish pakchoi from those areas. Results from this study presented a new method to discriminant the geographical origins of pakchoi, which could apply to other types of vegetables on the food market.

The enhancement of reactive red 24 adsorption from aqueous solution using agricultural waste-derived biochar modified with ZnO nanoparticles.

In this study, two types of agricultural wastes, sugarcane bagasse (SB) and cassava root husks (CRHs), were used to fabricate biochars. The pristine biochars derived from SB and CRHs (SBB and CRHB, respectively) were modified using ZnO nanoparticles to generate modified biochars (SBB-ZnO and CRHB-ZnO, respectively) for the removal of Reactive Red 24 (RR24) from stimulated wastewater. Batch experiments were performed to evaluate the effects of ZnO nanoparticles' loading ratio, solution pH, contact time, and initial RR24 concentration on the RR24 adsorption capacity of biochars. The RR24 adsorption isotherm and kinetic data on SBB, SBB-ZnO3, CRHB, and CRHB-ZnO3 were analyzed. Results indicate that SB- and CRH-derived biochars with a ZnO nanoparticle loading ratio of 3 wt% could generate maximum adsorption capacities of RR24 thanks to the double growth on the BET surface of modified biochars. The RR24 adsorption capacities of CRHB-ZnO3 and SBB-ZnO3 reached 81.04 and 105.24 mg g-1, respectively, which were much higher than those of pristine CRHB and SBB (66.19 and 76.14, respectively) at an initial RR24 concentration of 250 mg L-1, pH 3, and contact time of 60 min. The adsorption of RR24 onto biochars agreed well with the pseudo-first-order model and the Langmuir isotherm. The RR24 adsorption capacity on modified biochars, which were reused after five adsorption-desorption cycles showed no insignificant drop. The main adsorption mechanisms of RR24 onto biochars were controlled by electrostatic interactions between biochars' surface positively charged functional groups with azo dye anions, pore filling, hydrogen bonding formation, and π-π interaction.

Antibiotics in surface water of East and Southeast Asian countries: A focused review on contamination status, pollution sources, potential risks, and future perspectives.

This review provides focused insights into the contamination status, sources, and ecological risks associated with multiple classes of antibiotics in surface water from the East and Southeast Asia based on publications over the period 2007 to 2020. Antibiotics are ubiquitous in surface water of these countries with concentrations ranging from <1 ng/L to hundreds µg/L and median values from 10 to 100 ng/L. Wider ranges and higher maximum concentrations of certain antibiotics were found in surface water of the East Asian countries like China and South Korea than in the Southeast Asian nations. Environmental behavior and fate of antibiotics in surface water is discussed. The reviewed occurrence of antibiotics in their sources suggests that effluent from wastewater treatment plants, wastewater from aquaculture and livestock production activities, and untreated urban sewage are principal sources of antibiotics in surface water. Ecological risks associated with antibiotic residues were estimated for aquatic organisms and the prevalence of antibiotic resistance genes and antibiotic-resistant bacteria were reviewed. Such findings underline the need for synergistic efforts from scientists, engineers, policy makers, government managers, entrepreneurs, and communities to manage and reduce the burden of antibiotics and antibiotic resistance in water bodies of East and Southeast Asian countries.

Growth of Multiorientated Polycrystalline MoS2 Using Plasma-Enhanced Chemical Vapor Deposition for Efficient Hydrogen Evolution Reactions.

Molybdenum disulfide (MoS2) has attracted considerable attention as a promising electrocatalyst for the hydrogen evolution reaction (HER). However, the catalytic HER performance of MoS2 is significantly limited by the few active sites and low electrical conductivity. In this study, the growth of multiorientated polycrystalline MoS2 using plasma-enhanced chemical vapor deposition (PECVD) for the HER is achieved. The MoS2 is synthesized by sulfurizing a sputtered pillar-shaped Mo film. The relatively low growth temperature during the PECVD process results in multiorientated MoS2 with an expanded interlayer spacing of ~0.75 nm, which provides abundant active sites, a reduced Gibbs free energy of H adsorption, and enhanced intralayer conductivity. In HER applications, the PECVD-grown MoS2 exhibits an overpotential value of 0.45 V, a Tafel slope of 76 mV dec-1, and excellent stability in strong acidic media for 10 h. The high HER performance achieved in this study indicates that two-dimensional MoS2 has potential as an electrocatalyst for next-generation energy technologies.

Essential Oils of Lemongrass (Cymbopogon citratus Stapf) Induces Apoptosis and Cell Cycle Arrest in A549 Lung Cancer Cells.

Essential oils were extracted from the culm and leaf of Cymbopogon citratus collected from different regions of Vietnam and analyzed using GC/MS. The results showed that citral is the major component accounting for 61.20%-76.46% of the essential oils. The citral content was higher in the essential oil obtained from the leaf than in that from the culm of lemongrass in all regions. In particular, camphene, valerianol, and epi-α-muurolol can be used to differentiate essential oils originating from leaves versus culms. The cytotoxic effects of the essential oils on various lung cancer cell lines were evaluated in the present study. All essential oils exhibited cytotoxicity in the tested cells. The Ha Loc leaf essential oil (HLL) exhibited the most potent effects on A549 and H1975 cells, with IC50 values of 1.73 ± 0.37 and 4.01 ± 0.30 µg/mL, respectively. The Hy Cuong leaf essential oil (HCL) showed the strongest effect on H1299 cells, with an IC50 value of 2.45 ± 0.21 µg/mL. The Kim Duc culm (KDC) essential oil exerted the strongest cytotoxic effects against H1650 cells, with an IC50 value of 4.86 ± 0.29 µg/mL. The HLL induced apoptosis and cycle arrest in A549 cells according to flow cytometric analysis and fluorescent nuclear staining assays. The western blot analysis indicated that HLL induced the apoptotic effect by altering the regulating proteins of the apoptosis process such as caspase-3, Bcl-2, and Bax. The data strongly suggested that the intrinsic pathway may play an important role in the apoptotic effects of HLL.

Interaction between elevated temperature and different types of Na-salicylate treatment in Brachypodium dystachion.

Salicylic acid (SA) plays a role in several physiological processes in plants. Exogenously applied SA is a promising tool to reduce stress sensitivity. However, the mode of action may depend on how the treatment was performed and environmental conditions may alter the effects of SA. In the present study the physiological and biochemical effects of different modes of application (soaking seeds prior sowing; spraying leaves with 0.5 mM NaSA) were compared at normal and moderately elevated temperatures (4 h; 35°C) in Brachypodium distachyon (L.) P. Beauv. plants. While soaking the seeds stimulated plant growth, spraying caused mild stress, as indicated by the chlorophyll-a fluorescence induction parameters and changes in certain protective compounds, such as glutathione, flavonoids or antioxidant enzymes. Elevated temperature also caused an increase in the glutathione-S-transferase activity, and this increase was more pronounced in plants pre-treated with NaSA. Both seed soaking or spraying with NaSA and exposure to heat treatment at 35°C reduced the abscisic acid levels in the leaves. In contrast to abscisic acid, the jasmonic acid level in the leaves were increased by both spraying and heat treatment. The present results suggest that different modes of application may induce different physiological processes, after which plants respond differently to heat treatment. Since these results were obtained with a model plants, further experiments are required to clarify how these changes occur in crop plants, especially in cereals.

The potential health risks and environmental pollution associated with the application of plant growth regulators in vegetable production in several suburban areas of Hanoi, Vietnam.

Plant growth regulators (PGRs) play a pivotal role in increasing vegetable productivity, but they have many drawbacks that can include health and safe concerns of consumers. In this study, the plant growth regulators (PGRs) contents of 111 Brassica juncea (L.) Czern. samples collected from six main suburban areas of Hanoi, Vietnam, were determined by liquid chromatography-electrospray tandem mass spectrometry. Results showed the presence of PGRs residues in 83 analyzed samples (74.77%). Eight PGRs, including Indole-3-acetic acid (IAA), Indole-3-butyric acid (IBA), Indole-3-carboxylic acid (ICA), 3-Indolepropionic acid (IPA), Gibberellin A3 (GA3), Gibberellin A4 (GA4), Gibberellin A7 (GA7) and Trans-zeatin (tZ), were found in B. juncea with the average residues dropped in the range of 0.04 and 0.65 mg kg-1. GA4 was considered as the most popular PGR applying in vegetable production in the investigated areas. Notably, the concentrations of gibberellins were higher than the regulations in vegetables and fruits in Europe, USA and Japan. This might be the potential health risks to consumers and environmental pollution, which necessary to be controlled with consideration of residue regulations.

Highly sensitive and rapid determination of sunset yellow in drinks using a low-cost carbon material-based electrochemical sensor.

Starting from simple graphite flakes, an electrochemical sensor for sunset yellow monitoring is developed by using a very simple and effective strategy. The direct electrochemical reduction of a suspension of exfoliated graphene oxide (GO) onto a glassy carbon electrode (GCE) surface leads to the electrodeposition of electrochemically reduced oxide at the surface, obtaining GCE/ERGO-modified electrodes. They are characterized by cyclic voltammetry (CV) measurements and field emission scanning electron spectroscopy (FE-SEM). The GCE/ERGO electrode has a high electrochemically active surface allowing efficient adsorption of SY. Using differential pulse voltammetry (DPV) technique with only 2 min accumulation, the GCE/ERGO sensor exhibits good performance to SY detection with a good linear calibration for concentration range varying 50-1000 nM (R2 = 0.996) and limit of detection (LOD) estimated to 19.2 nM (equivalent to 8.9 µg L-1). The developed sensor possesses a very high sensitivity of 9 µA/µM while fabricated with only one component. This electrochemical sensor also displays a good reliability with RSD value of 2.13% (n = 7) and excellent reusability (signal response change < 3.5% after 6 measuring/cleaning cycles). The GCE/ERGO demonstrates a successful practical application for determination of sunset yellow in commercial soft drinks. Graphical abstract.

Multiresidue Pesticides Analysis of Vegetables in Vietnam by Ultrahigh-Performance Liquid Chromatography in Combination with High-Resolution Mass Spectrometry (UPLC-Orbitrap MS).

An ultrahigh-performance liquid chromatography in combination with high-resolution mass spectrometry Thermo Q-Extractive Focus Orbitrap MS has been introduced for analysis of multiclass pesticides in vegetable samples collected in Hanoi, Vietnam. Multiclass pesticides were separated on the Thermo Hypersil Gold PFP column utilizing a gradient of the mobile phase consisting of 5 mM ammonium formate, 0.1% formic acid in deionized water, and methanol. The target analytes were detected in the full-scan mode on Thermo Scientific Q-Exactive Focus Orbitrap MS for quantitation at the optimum operating conditions. These conditions included, but not limit to, the resolution of 70000 at the full width at half maximum in both positive and negative mode, mass range from 80 to 1000 m/z, and optimized parameters for the heated electrospray ionization source. The identification of the analytes in real samples was based on retention times, mass to charge ratios, mass accuracies, and MS/MS spectra at the confirmation mode with the inclusion list of target analytes. The mass accuracies of target analytes were from -4.14 ppm (dinotefuran) to 1.42 ppm (cinosulfuron) in the neat solvent and from -3.91 ppm (spinosad D) to 1.29 ppm (cinosulfuron) in the matrix-matched solution. Target analytes in the vegetable-based matrix were extracted by the QuEChERS method. Some critical parameters of the analytical method such as linearity, repeatability, limit of detection, and limit of quantitation have been evaluated and implemented. Excellent LOD and LOQ of the developed method were achieved at the range of 0.04-0.85 and 0.13-2.9 µg·kg-1, respectively. Intraday and interday repeatability of the analytical signal (peak area, n=6) of the developed method were below 3% and 10%, correspondingly. The matrix effect, extraction recovery, and overall recovery were fully investigated by spiking experiments. Experimental results demonstrated that the ionization suppression or enhancement was the main contribution on the overall recoveries of target analytes. Finally, the in-house validated method was applied to pesticides screening in vegetables samples in local villages in Hanoi, Vietnam. The concentrations of all target analytes were below limit of quantitation and lower than US-FDA or EU maximum residue levels.

Synthesis and anti-tubercular activity of N(2)-arylbenzo[g]isoquinoline-5,10-dione-3-iminium bromides.

Tuberculosis has remained a challenge for medicinal chemists worldwide. In the framework of a collaborative program to identify and evaluate novel antitubercular candidate compounds, the biological properties of benzo[g]isoquinoline-5,10-diones have been found to be very promising. In this paper we have further expanded the library by incorporation of an amidinium moiety into the benzo[g]isoquinoline-5,10-dione scaffold. The presence of this functional group also increased the solubility of the quinones in polar solvents. To this purpose N(2)-arylbenzo[g]isoquinoline-5,10-dione-3-iminium bromides were synthesized in a straightforward way by means of a reaction of anilines with 2-(bromomethyl)-3-(cyanomethyl)-1,4-dimethoxynaphthalene. Following the biological evaluation, N(2)-(4-chlorophenyl)-5,10-dioxobenzo[g]isoquinoline-3(2H)-iminium bromide (MIC = 1.16 µM, CC50 = 28.51 µM, SI = 24.58) was selected as the most promising representative. Apart from the nano-molar anti-mycobacterial activity, the compound was able to target intracellular residing Mycobacterium tuberculosis and the susceptibility of a multi-drug-resistant strain towards the compound was confirmed.

Dichlorido[(R,R)-N,N,N-tribenzyl-cyclo-hexane-1,2-diamine-κN,N]copper(II).

In the title compound, [CuCl(2)(C(27)H(32)N(2))], which bears a chiral diamine ligand, viz (R,R)-N,N,N''- tribenzyl-cyclo-hexane-1,2-diamine, the Cu(II) ion is ligated by two N and two Cl atoms in a distorted square-planar geometry. The coordination of the ligands to the Cu(II) ion results in the formation of a five-membered heterocyclic ring and a chiral center at the monosubstituted nitro-gen in an (S)-configuration. The catalytic capacity of the complex for the asymmetric nitro-aldol reaction is promising (49% ee).