Fusarium solani PQF9 Isolated from Podocarpus pilgeri Growing in Vietnam as a New Producer of Paclitaxel.

Endophytic fungi are known as an alternative promising source of anticancer drug, paclitaxel, however fungi inhabiting in medicinal plant Podocarpus pilgeri and their paclitaxel production have not been reported to date. In the present study, a total of 15 culturable fungi classified into 5 genera, were successfully recovered from P. pilgeri collected in Vietnam. Screening fungal dichloromethane extracts for anticancer activity revealed that only PQF9 extract displayed potent inhibitory effects on A549 and MCF7 cancer cell lines with IC50 values of 33.9 ± 2.3 µg/mL and 43.5 ± 1.7 µg/mL, respectively. Through PCR-based molecular screening, the isolate PQF9 was found to possess 3 key genes involved in paclitaxel biosynthesis. Importantly, high-performance liquid chromatography quantification showed that fungal isolate PQF9 was able to produce 18.2 µg/L paclitaxel. The paclitaxel-producing fungus was identified as Fusarium solani PQF9 based on morphological and molecular phylogenetic analysis. Intensive investigations by chromatographic methods and spectroscopic analyses confirmed the presence of paclitaxel along with tyrosol and uracil. The pure paclitaxel had an IC50 value of 80.8 ± 9.4 and 67.9 ± 7.0 nM by using cell viability assay on A549 lung and MCF7 breast cancer cells. In addition, tyrosol exhibited strong antioxidant activity by scavenging 2, 2-diphenyl-picrylhydrazyl (DPPH) (IC50 5.1 ± 0.2 mM) and hydroxyl radical (IC50 3.6 ± 0.1 mM). In contrast, no biological activity was observed for uracil. Thus, the paclitaxel-producing fungus F. solani PQF9 could serve as a new material for large-scale production and deciphering paclitaxel biosynthesis. Supplementary Information: The online version contains supplementary material available at 10.1007/s12088-023-01119-z.

Take care when utilize antibiotics

Antibiotic use must carefully and must exactly consider according to indication and combined exactly method and disease. To against antibiotic resistance, urgent methods is needed such as drugs used exactly disease, sufficient dose and enough time. For physicians, should careful if prescribe antibiotic, don't use new drug if older drug still effective, review antibiotic resistance to choose exactly, preservation obey exact standards, limited using antibiotic for cattle and poultry

Ecstasy – is very dangerous

Ecstasy has been registered in 1914 as an anti-tired medicament for military use. From the year 1980, Ecstasy becomes an excitant using in amusing with the name of “shaking drug”, “Rabid drug”. After consumption, users become cheery, infatuated, vigilant, untiring, loosing of the feel of hunger, fond of groping for and embracing, drinking alcohol and beer, dancing in all night. In the state of passionately fond the user can kill and rape. After a stage of exciting, the user can get severe and long weakening, get psychologic disturbance, get acute hepatitis. The harmfulness of ecstasy can be continuously listed