Nutritional and Functional Properties of Defatted Flour, Protein Concentrates, and Isolates of Brachytrupes membranaceus (Orthoptera: Gryllidae) (Drury: 1773) and Macrotermes subhyalinus (Isoptera: Blattodea) (Rambur: 1842) from Burkina Faso.

Brachytrupes membranaceus and Macrotermes subhyalinus are edible insects in Burkina Faso. Our research aimed to evaluate the nutritional composition and functional properties of the defatted flours, protein concentrates, and isolates of Brachytrupes membranaceus and Macrotermes subhyalinus. Proximate and mineral composition were determined according to AOAC methods. The amino acid and fatty acid composition were determined by high-performance liquid chromatography (HPLC) and gas chromatography, respectively. The protein concentrates and isolates were obtained by solubilization, precipitation, and lyophilization. Macrotermes subhyalinus showed the highest protein (45.75 g/100 g), iron (11.76 mg/100 g), and zinc (13.18 mg/100 g) contents. The highest isoleucine and lysine contents, the best fat absorption (10.87 g/g), and foaming capacities (49.60%) were obtained with the isolate of Brachytrupes membranaceus. Consumption of Macrotermes subhyalinus could be used to fight or correct iron and zinc deficiencies. Macrotermes subhyalinus was a source of macronutrients and micronutrients, while the protein concentrates and isolates of Brachytrupes membranaceus were endowed with functional properties (fat absorption and foaming capacities).

Antifungal and Antiaflatoxinogenic Effects of Cymbopogon citratus, Cymbopogon nardus, and Cymbopogon schoenanthus Essential Oils Alone and in Combination.

The antifungal and antiaflatoxinogenic activities of the essential oils (EOs) from the leaves of Cymbopogon schoenanthus, Cymbopogon citratus, Cymbopogon nardus, and their pair combinations were investigated. Antifungal susceptibility and the efficacy of paired combinations of EOs were assessed using agar microdilution and checkerboard methods, respectively. Identification and quantification of chemical components of the EOs were carried out by gas chromatography-mass spectrometry and gas chromatography-flame ionization detector (GC-MS and GC-FID), respectively. Aflatoxins were separated and identified by High-Performance Liquid Chromatography (HPLC) and then quantified by spectrofluorescence. The EO of C. nardus exhibited the highest inhibitory activity against Aspergillus flavus and Aspergillus parasiticus. The combination of C. citratus and C. nardus and that of C. nardus and C. schoenanthus exhibited a synergistic effect against Aspergillus flavus and Aspergillus, respectively. Both C. citratus and C. schoenanthus EOs totally inhibited the synthesis of aflatoxin B1 at 1 µL/mL. C. citratus blocked the production of aflatoxins B2 and G2 at 0.5 µL/mL. Both C. citratus and C. schoenanthus totally hampered the production of the aflatoxin G1 at 0.75 µL/mL. The combination of C. citratus and C. schoenanthus completely inhibited the production of the four aflatoxins. The study shows that the combinations can be used to improve their antifungal and antiaflatoxinogenic activities.