Attitudes and prevention towards malaria in the context of COVID-19 pandemic in urban community in Benin, West Africa.

BACKGROUND: The COVID-19 pandemic and its damages have severely impacted the global healthcare system even in countries with the best systems. In sub-Saharan Africa (SSA), it could worsen the malaria situation in endemic countries such as Benin. This study was conducted to describe the potential effects of the pandemic on urban dwellers attitudes, prevention and treatment against malaria in four major cities of Benin. METHODS: A cross-sectional questionnaire survey was conducted in Cotonou, Bohicon, Parakou and Natitingou, four urban cities of Benin. A total of 800 randomly selected households were interviewed. The questionnaire consisted of four parts: sociodemographic characteristics, knowledge, attitude, and practice. Descriptive statistics and binomial logistic regression analysis were used in the statistical analysis. RESULTS: More than 90% of the participants interviewed had a good level of knowledge about the transmission and prevention of malaria in the cities surveyed. In contrast, low proportions of participants reported visiting health-care facilities when they suspected malaria. Compared to the proportions observed at Parakou and Natitingou, the low proportion of participants was statistically significant at Cotonou (Parakou: X2 = 31.345, df = 1, P < 0.0001; Natitingou: X2 = 17.471, df = 1, P < 0.0001). Among the reasons for not seeking care, these related to COVID-19 were the most mentioned. Moreover, the good education level of the participants was one of the factors associated with the non-use of healthcare facilities due to over-knowledgeable about Covid-19, which might have increased the fear to go to the health facilities. Finally, high proportions of self-medication practice were mentioned with high use of malaria drugs to treat both malaria and to protect against COVID-19. CONCLUSIONS: The data show a negative impact of COVID-19 on visits to healthcare facilities for malarial treatment and malaria drugs usage by the population. It is, therefore, necessary to rebuild malaria programmes by integrating measures adapted to health crises such as the COVID-19 pandemic.

Diversity of larval habitats of Anopheles mosquitoes in urban areas of Benin and influence of their physicochemical and bacteriological characteristics on larval density.

BACKGROUND: The implementation of anti-larval strategies in the fight against malaria vectors requires fundamental knowledge of their oviposition sites. The aim of this study was to assess the spatial and temporal distribution of Anopheles breeding sites as well as the influence of abiotic and biotic factors on the proliferation of larvae in urban and non-urban areas of Benin. METHODS: Sampling of Anopheles larvae was carried out during the rainy and dry seasons in urbanized and non-urbanized areas of the cities of Cotonou, Bohicon, Parakou, and Natitingou in Benin. The Anopheles larval breeding sites were georeferenced and characterized by their nature, type, physicochemical (pH, temperature, dissolved oxygen, conductivity, turbidity, salinity) and biological attributes (larval density and coliform density). RESULTS: A total of 198 positive breeding sites for Anopheles larvae were identified, comprising 163 (82.3%) in the rainy season and 35 (17.7%) in the dry season. Out of these larval habitats, 61.9% were located in urbanized areas, and were predominantly puddles. Principal component analysis revealed a high positive correlation of larval density with temperature and dissolved oxygen, and with salinity in the coastal zone. In addition, cross-sectional analysis of the microbiological results with larval density showed a significant negative correlation between larval productivity and faecal coliform load. CONCLUSIONS: This study indicated the presence of multiple larval habitats of Anopheles in the urban areas which were created through human activities, and associations between larval density and intrinsic factors of the habitats such as temperature, dissolved oxygen and faecal coliform load. This type of information may be useful for the implementation of appropriate control strategies in urban areas, including regulation of the human activities that lead to the creation of breeding sites, proper environmental management and targeted larvicidal use.

Effects of KMnO4 amounts on antibacterial properties of activated carbon for efficient treatment of northern Benin hospital wastewater in a fixed bed column system.

In this study, the wastewater from the Departmental Hospital Center of Atacora in Benin was characterized and then treated with activated carbon/potassium permanganate (AC/KMnO4) composite in a fixed bed column system. The AC/KMnO4 composites with impregnation ratios range 0.025-0.100 were prepared from peanut shell activated carbon and potassium permanganate. The wastewater characteristics revealed that 75% of Escherichia coli strains identified were extended-spectrum lactamases (ESBL) with CTX-M dominance, while 25% of Staphylococcus aureus strains produced Panton and Valentine leucocidin and 77.80% of Salmonella typhi strains were resistant to Trimethoprim/Sulfamethoxazole. The fixed bed column system results showed removal efficiency of 72.18 ± 4.98% turbidity, 63.12 ± 4.11% COD, 0.70 ± 0.04 log10 against E. coli and 3.82 ± 0.01 log10 against S. typhi strains using activated carbon as adsorbent with 0.7 cm bed depth after 3 h of treatment. The composite adsorbent AC/KMnO4 significantly increased the effectiveness of treatment due to the strong oxidant power of KMnO4 in the composite material. The results depicted a removal rate of 83.88 ± 5.00%, 89 ± 1.95%, 90 ± 0.65% turbidity, 66.47 ± 1.62%, 69.82 ± 2.00%, 78.20 ± 2.82% COD, 2.0 ± 0.08 log10, 5.0 ± 0.07 log10 against E. coli and 3.82 ± 0.01 log10 against S. typhi strains using AC/KMnO4 with 0.025, 0.050 and 0.100 ratios respectively at 0.7 cm bed depth. Finally, AC/KMnO4 revealed more adsorption potential and antibacterial property than AC, hence, the composite material could be used as a cost-effective adsorbent for efficient removing of multi-resistant bacteria from hospital wastewater.