Honey Bee Products as Bio Indicator of Heavy Metals Pollution and Health Risk Assessment Through the Consumption of Multifloral Honey Collected in Azad Kashmir, Pakistan.

The study assessed the health risks associated with heavy metal ingestion and explored the use of honey bee products as a bio-indicator for heavy metal pollution. All honey bee products tested showed heavy metals, but some honey samples had concentrations exceeding permissible limits for Cd, Pb, Ni, and Cr. The mean concentrations of heavy metals (mg/kg) in the honey, propolis, bee wax, and bee pollen were Fe (1.32) > Zn (1.31) > Pb (0.46) > Ni (0.18) > Cr (0.16) > Cu (0.14) > Co (0.12) > Mn (0.05) > Cd (0.03), Fe (8) > Zn (1.13) > Mn (0.59) > Pb (0.13) > Ni (0.07) > Cu (0.06) > Co (0.05) > Cr (0.03) > Cd (0.02), Fe (1.31) > Pb (0.41) > Ni (0.407) > Zn(0.25) > Mn (0.12) > Co(0.10) > Cu (0.07) > Cr (0.05) > Cd (0.002), and Fe (2.2) > Zn (0.75) > Ni (0.25) > Pb (0.16) > Cu (0.05) > Mn (0.045) > Co (0.04) > Cr (0.01) > Cd(0.002), respectively. Similarly, the mean concentration of heavy metals (mg/kg) in the soil, flowers and pine pollen was Fe (539.08) > Zn (89.53) > Mn (66.91) > Ni (58.5) > Co (19.2) > Cr (11.42) > Pb (6.58) > Cu (5.71) > Cd (0.19), Fe (3.12) > Zn (0.95) > Mn (0.72) > Ni (0.29) > Cu (0.16) > Cr (0.14) > Pb (0.059) > Co (0.057) > Cd (0.003) and Fe (2.59) > Zn (1.75) > Mn (0.43) > Pb (0.34) > Co (0.1) > Cr (0.07) > Cu (0.06) > Cd (0.039) > Ni (0.03), respectively. The atomic absorption spectrophotometry procedure was validated through a recovery study and achieved accuracy through the limit of detection (LOD) and limit of quantification (LOQ). The mean Bio concentration factor (BCF) indicated that the transfer from soil to honey was higher than from soil to flower. The metal pollution index (MPI) of the selected indicators was in descending order: soil > honey > flowers > propolis > pine pollen > beeswax > bee pollen. The hazard quotient (HQ) and hazard index (HI) were below one, showing no chronic health risk. The carcinogenic risk (CR) of Cd, Cr, and Ni in honey for children, male and female adults for the consumers exceeds the acceptable level, making Cd, Cr, and Ni the most concerning heavy metals in honey. The study suggests that regular monitoring of heavy metal pollution is essential.

Root system architecture associated zinc variability in wheat (Triticum aestivum L.).

Root system architecture (RSA) plays a fundamental role in nutrient uptake, including zinc (Zn). Wheat grains are inheritably low in Zn. As Zn is an essential nutrient for plants, improving its uptake will not only improve their growth and yield but also the nutritional quality of staple grains. A rhizobox study followed by a pot study was conducted to evaluate Zn variability with respect to RSA and its impact on grain Zn concentration. The grain Zn content of one hundred wheat varieties was determined and grown in rhizoboxes with differential Zn (no Zn and 0.05 mg L-1 ZnSO4). Seedlings were harvested 12 days after sowing, and root images were taken and analyzed by SmartRoot software. Using principal component analysis, twelve varieties were screened out based on vigorous and weaker RSA with high and low grain Zn content. The screened varieties were grown in pots with (11 mg ZnSO4 kg-1 soil) and without Zn application to the soil. Zinc translocation, localization, and agronomic parameters were recorded after harvesting at maturity. In the rhizobox experiment, 4% and 8% varieties showed higher grain Zn content with vigorous and weaker RSA, respectively, while 45% and 43% varieties had lower grain Zn content with vigorous and weaker RSA. However, the pot experiment revealed that varieties with vigorous root system led to higher grain yield, though the grain Zn concentration were variable, while all varieties with weaker root system had lower yield as well as grain Zn concentration. Zincol-16 revealed the highest Zn concentration (28.07 mg kg-1) and grain weight (47.9 g). Comparatively higher level of Zn was localized in the aleurone layer than in the embryonic region and endosperm. It is concluded that genetic variability exists among wheat varieties for RSA and grain Zn content, with a significant correlation. Therefore, RSA attributes are promising targets for the Zn biofortification breeding program. However, Zn localization in endosperm needs to be further investigated to achieve the goal of reducing Zn malnutrition.

Spatial variance and estimation of nitrogen dioxide levels as a contributing factor to asthma epidemiology in Rawalpindi, Pakistan.

Asthma prevalence and morbidity are increasing rapidly worldwide, especially in developing countries. Previous studies have shown nitrogen dioxide as an important contributor to asthma prevalence along with extreme temperatures, relative humidity, and land use change. The present study aimed to assess the asthma epidemiology and association of nitrogen dioxide, temperature, and land use as a contributing factor for increasing asthma prevalence in Rawalpindi, Pakistan. Secondary data related to the frequency of asthmatics hospital visits were analyzed to figure out the hotspots of asthma by using Getis ord Gi* statistics in ArcGIS 10.2. Moreover, intraurban variation of nitrogen dioxide concentration was analyzed by passive sampling method and its association with the rate of asthmatics hospital visits in Rawalpindi, Pakistan was also researched. Results revealed the random distribution of disease with significant hotspots along with spatial variability of nitrogen dioxide in urban and rural locations. Indoor and outdoor levels of nitrogen dioxide exceed the national and world health organization standards on asthma high risk areas especially in winter season. Congested housing with poor ventilation, unplanned urbanization, cold temperature, and unclean fuel use are revealed as strong determinants of asthma prevalence in Rawalpindi, Pakistan. Extensive monitoring and interventions are needed for the reduction of both indoor and outdoor nitrogen dioxide levels to overcome the increasing rate of asthma prevalence.

Concentration and Localization of Fe and Zn in Wheat Grain as Affected by Its Application to Soil and Foliage.

Nutritional status of people can be improved by enhancing zinc (Zn) and iron (Fe) content in cereals used as staple mainly in poor resource countries. Zinc and Fe were applied through soil and foliage in a study to biofortify wheat grains. Foliar application of both micronutrients increased the growth and grain vigor as compared to soil application and control. Also, foliar application significantly enhanced Zn and Fe concentration in grain pre-dominantly localized in aleurone layer. Exogeneous application of Fe and Zn was found beneficial for plant growth and enhanced Fe and Zn concentrations in grain, however aleurone layer and embryonic region of the grain showed higher accumulations than that in endosperm. Therefore, understanding of physiological and molecular pathways for uptake and localization of Fe and Zn in wheat grains need to be critically examined to improve their concentration in grain to achieve the biofortification targets.

Scanning electron imaging of mellitophilous and allergenic pollen grain of arid and northern irrigated agroecological zones of Pakistan.

Palynomorphic studies of 65 common mellitophilous and 16 allergenic flora of Arid and Northern irrigated agroecological zones of Pakistan are carried out in this study by using field emission scanning electron microscope (FESEM). Mellitophilous pollen were extracted from honey samples of selected sites. For collection of local allergenic pollen, previously identified allergy-causing plants were selected. Pollen morphological examination was carried out under FESEM. Diverse range of pollen shapes ranging from monad to polyad and sculpturing ranging from psilate to echinate, scabrate to reticulate, bireticulate, or echinolophate were observed. Brassicaceae, Myrtaceae, Asteraceae, Rosaceae, and Poaceae were observed to be dominant allergenic and mellitophilous families of the selected sites.

Ethnoveterinary health management practices using medicinal plants in South Asia - a review.

Animal rearing is the major occupation of most population of South Asian countries. Due to lack of resources and limited approach to modern medicine, most of the livestock raisers prefer to use plant-based traditional medicine also referred to as ethnoveterinary medicine (EVM). Indeed, the use of medicinal plants in South Asia dates back to several centuries with documented evidences. However, there is currently a dearth of documentation and compilation of use of medicinal plants for animal diseases in this part of the world. This review aims to provide an up-to-date compilation of common medicinal plants used for the treatment and/or management of common animal diseases in South Asian countries. Extensive literature search was conducted online and relevant data was retrieved from well-known scientific databases. A total of 276 plants belonging to 95 families have been documented to be in common use for managing 14 different categories of animal diseases. Solanaceae, Lamiaceae, Fabaceae, and Leguminosae were most common plant families in terms of their plant species used for EVM. Gastric diseases were commonly reported and accounted for 72 species of plants used for its treatment followed by the miscellaneous disorders category and skin diseases comprising of 65 and 39 plant species respectively. Herbs accounted for 46% of the total plant species, followed by trees (33%), and shrubs (18%). The EVM were applied through different routes of administration; oral administration accounted for 72% followed by topical application 27%, while burning of plant parts to create smoke around animals to repel insects was less common (1%). It is anticipated that the present review will stimulate further ethnoveterinary research among livestock disease management practices in South Asia.

Estimation of Anticipated Performance Index and Air Pollution Tolerance Index and of vegetation around the marble industrial areas of Potwar region: bioindicators of plant pollution response.

Mitigating industrial air pollution is a big challenge, in such scenario screening of plants as a bio monitor is extremely significant. It requires proper selection and screening of sensitive and tolerant plant species which are bio indicator and sink for air pollution. The present study was designed to evaluate the Air Pollution Tolerance Index (APTI) and Anticipated Performance Index (API) of the common flora. Fifteen common plant species from among trees, herb and shrubs i.e. Chenopodium album (Chenopodiaceae), Parthenium hysterophorus (Asteraceae), Amaranthus viridis (Amaranthaceae), Lantana camara (Verbenaceaea), Ziziphus nummulari (Rhamnaceae), Silibum merianum (Asteraceae), Cannabis sativa (Cannabinaceae), Calatropis procera (Asclepediaceae), Ricinus communis (Euphorbiaceae), Melia azadirachta (Meliaceae), Psidium guajava (Myrtaceae), Eucalyptus globules (Myrtaceae), Broussonetia papyrifera (Moraceae), Withania somnifera (Solanaceae) and Sapium sabiferum (Euphorbiaceae) were selected growing frequently in vicinity of Marble industries in Potwar region. APTI and API of selected plant species were analyzed by determining important biochemical parameter i.e. total chlorophyll, ascorbic acid, relative water content and pH etc. Furthermore the selected vegetation was studied for physiological, economic, morphological and biological characteristics. The soil of studied sites was analyzed. It was found that most the selected plant species are sensitive to air pollution. However B. papyrifera, E. globulus and R. communis shows the highest API and therefore recommended for plantation in marble dust pollution stress area.

Evidence that a very brief psychological intervention boosts weight loss in a weight loss program.

Reducing morbidity and mortality associated with being overweight is a crucial public health goal. The aim of the present research was to test the efficacy of a very brief psychological intervention (a volitional help sheet) that could be used as an adjunct to standard weight loss programs to support increased weight loss in an overweight sample. Seventy-two overweight participants currently participating in a weight loss program were randomly allocated to either an intervention (volitional help sheet) condition or a control (distracter task) condition. The main outcome measure was weight at 1-month follow-up. Participants in both conditions lost significant amounts of weight, but those in the intervention condition lost significantly more than those in the control condition (d=0.66). The findings support the efficacy of the volitional help sheet to promote additional weight loss in an overweight sample engaged in a weight loss program. The volitional help sheet therefore represents a very brief, low-cost intervention that could be used to supplement ongoing weight-loss programs.