Subject(s)
Antioxidants/chemistry , Indole Alkaloids/chemistry , Monoterpenes/chemistry , Rubiaceae/chemistry , Antioxidants/isolation & purification , Antioxidants/pharmacology , Crystallography, X-Ray , Indole Alkaloids/isolation & purification , Indole Alkaloids/pharmacology , Models, Molecular , Molecular Structure , Monophenol Monooxygenase/antagonists & inhibitors , Monoterpenes/isolation & purification , Monoterpenes/pharmacology , Plant Bark/chemistry , Plant Extracts/chemistry , Plant Extracts/isolation & purification , Plant Extracts/pharmacologyABSTRACT
Phytochemical investigation of the seeds of Salacia longipes var. camerunensis led to the isolation of four sesquiterpenoid derivatives, salaterpene A (1) (1α,2ß,8ß-triacetoxy-6ß,9ß-dibenzoyloxy-4ß-hydroxy-dihydro-ß-agarofuran), salaterpene B (2) (1α,2ß,8ß-triacetoxy-9ß-benzoyloxy-6ß-cinnamoyloxy-4ß-hydroxy-dihydro-ß-agarofuran), salaterpene C (3) (1α,2ß-diacetoxy-6ß,9ß-dibenzoyloxy-4ß-hydroxy-dihydro-ß-agarofuran) and salaterpene D (4) (2ß-acetoxy-1α,6ß-dibenzoyloxy-4ß-hydroxy-9ß-nicotinoyloxy-dihydro-ß-agarofuran) together with two known compounds (5 and 6). The structures of the compounds were established by means of NMR spectroscopy. Compounds 1-4 and 6 were tested in vitro for their antiplasmodial activity against Plasmodium falciparum chloroquine-resistant strain W2. All the tested compounds exhibited a moderate potency with IC50 below 2.7 µM.
Subject(s)
Chloroquine/pharmacology , Plasmodium falciparum/drug effects , Salacia/chemistry , Sesquiterpenes/isolation & purification , Sesquiterpenes/pharmacology , Cameroon , Disease Resistance/drug effects , Erythrocytes/drug effects , Humans , Inhibitory Concentration 50 , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Seeds/chemistry , Sesquiterpenes/chemistryABSTRACT
Phytochemical investigation of the figs of Ficus mucuso led to the isolation of three new isoflavone dimer derivatives, mucusisoflavones A-C (1-3), together with 16 known compounds. Some of the isolates were tested in vitro for their inhibitory properties toward ß-glucuronidase and Plasmodium falciparum enoyl-ACP reductase (PfENR) enzymes. Compound 1 (IC50) 0.68 µM) showed inhibitory activity on ß-glucuronidase enzyme, while 3 (IC50) 7.69 µM) exhibited a weak inhibitory activity against P. falciparum enoyl-ACP reductase (PfENR).
Subject(s)
Antimalarials/isolation & purification , Antimalarials/pharmacology , Enoyl-(Acyl-Carrier-Protein) Reductase (NADH)/antagonists & inhibitors , Ficus/chemistry , Glucuronidase/antagonists & inhibitors , Isoflavones/isolation & purification , Isoflavones/pharmacology , Plasmodium falciparum/drug effects , Plasmodium falciparum/enzymology , Antimalarials/chemistry , Cameroon , Inhibitory Concentration 50 , Isoflavones/chemistry , Molecular StructureABSTRACT
A phytochemical study of the stem bark of Vismia laurentii resulted in the isolation of a tetracyclic triterpene, tirucalla-7,24-dien-3-one (1), and seven other known compounds: 3-geranyloxyemodin (2), vismiaquinone A (3), vismiaquinone B (4), bivismiaquinone (5), epifriedelinol (6), betulinic acid (7) and stigmasta-7,22-dien-3-ol (8). The structure of all these compounds was elucidated by spectroscopic means. The stem bark extract and compounds 1 and 3 showed good antimalarial activity against the W2 strain of Plasmodium falciparum.
Subject(s)
Antimalarials/isolation & purification , Clusiaceae/chemistry , Plant Stems/chemistry , Anthracenes/chemistry , Anthracenes/isolation & purification , Anthracenes/toxicity , Antimalarials/chemistry , Antimalarials/toxicity , Cameroon , Erythrocytes/drug effects , Erythrocytes/physiology , HumansABSTRACT
Two anthraquinones, zenkequinones A and B were isolated from the stem bark of Stereospermum zenkeri together with known sterequinone-F, p-coumaric acid, sitosterol-3-O-beta-D-glucopyranoside and 3beta-hydroxyolean-12-en-28-O-beta-D-glucopyranoside. Their structures were established by spectroscopic methods. The antimicrobial activity of the isolated compounds was evaluated against six multiresistant strains of pathogens. Zenkequinone B showed the best antibacterial activity (MIC 9.50 microg/ml) against gram-negative Pseudomonas aeruginosa.
