ABSTRACT
The focus of high-throughput drug discovery has progressed through the genome and the transcriptome and is now moving towards more difficult problems in assessing the proteome, glycome and metabolome. Microarrays are currently the major tool in the assessment of gene expression via cDNA or RNA analysis; however, they are also used to screen libraries of proteins and small molecules. Microarrays have helped to extract more information from smaller sample volumes and enabled the incorporation of low-cost high-throughput assays in the drug discovery process. The technology continues to develop and is being rapidly transferred into more challenging areas, with the potential to further aid and enhance the drug discovery process through the development of, for example, proteomic, glycomic and tissue arrays.
Subject(s)
Microarray Analysis/methods , Animals , Gene Expression Profiling , Humans , Microfluidic Analytical Techniques/methods , Nanotechnology , Oligonucleotide Array Sequence Analysis/methods , Protein Array Analysis/methods , Tissue Array Analysis/methodsABSTRACT
The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.
Subject(s)
Biotin/chemistry , Calorimetry/methods , Coated Materials, Biocompatible/chemistry , Polymerase Chain Reaction/instrumentation , Polymerase Chain Reaction/methods , Polypropylenes , Spectrometry, Fluorescence/methods , Streptavidin/chemistry , Equipment Failure Analysis/methods , Macromolecular Substances , Protein Binding , Protein Interaction Mapping/methodsABSTRACT
The focus of high-throughput drug discovery has progressed through the genome and the transcriptome and is now moving towards more difficult problems in assessing the proteome, glycome and metabolome. Microarrays are currently the major tool in the assessment of gene expression via cDNA or RNA analysis; however, they are also used to screen libraries of proteins and small molecules. Microarrays have helped to extract more information from smaller sample volumes and enabled the incorporation of low-cost high-throughput assays in the drug discovery process. The technology continues to develop and is being rapidly transferred into more challenging areas, with the potential to further aid and enhance the drug discovery process through the development of, for example, proteomic, glycomic and tissue arrays.