ABSTRACT
Three different glucans (PS-I, PS-II, and PS-III) were isolated from the alkaline extract of the fruiting bodies of an edible mushroom Pleurotus florida, cultivar Assam Florida. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of these polysaccharides was established as follows: [Formula: see text].
Subject(s)
Glucans/chemistry , Glucans/isolation & purification , Pleurotus/chemistry , Carbohydrate Sequence , Methylation , Molecular Sequence Data , Periodic Acid/chemistryABSTRACT
A water-soluble polysaccharide (SMPS) isolated from the aqueous extract of the green (unripe) fruits of Solenum melongena (Brinjal) contains D-galactose, D-methyl galacturonate, 3-O-acetyl D-methyl galacturonate, and L-arabinose in a molar proportion of nearly 1:1:1:1. Structural investigation of the polysaccharide was carried out using total acid hydrolysis, methylation analysis, periodate oxidation study, and NMR studies ((1)H, (13)C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of the above experiments the structure of the repeating unit of the polysaccharide (SMPS) was established as -->5)-alpha-L-Araf-(1-->4)-beta-D-Galp-(1-->2)-alpha-D-3-O-Ac-GalpA6Me-(1-->2)alpha-D-galpA6Me-(1--> This molecule showed splenocyte and thymocyte activations.
Subject(s)
Fruit/chemistry , Polysaccharides/chemistry , Solanum melongena/chemistry , Carbohydrate Sequence , Molecular Sequence Data , Nuclear Magnetic Resonance, Biomolecular , Polysaccharides/isolation & purificationABSTRACT
A polysaccharide (PS-I) isolated from the aqueous extract of the unripe (green) tomatoes (Lycopersicon esculentum) consists of D-galactose, D-methyl galacturonate, D-arabinose, L-arabinose, and L-rhamnose. Structural investigation of the polysaccharide was carried out using total acid hydrolysis, methylation analysis, periodate oxidation study, and NMR studies ((1)H, (13)C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of above-mentioned experiments the structure of the repeating unit of the polysaccharide (PS-I) was established as: [structure: see text].
Subject(s)
Polysaccharides/chemistry , Solanum lycopersicum/chemistry , Carbohydrate Sequence , Solanum lycopersicum/growth & development , Magnetic Resonance Spectroscopy , Molecular Sequence Data , Polysaccharides/isolation & purification , Water/chemistryABSTRACT
A water-soluble protein containing glucan was isolated from an edible mushroom, Termitomyces microcarpus (var). It was found to contain 55% of carbohydrate and 40% of protein. The protein part was found to consist of glutamine, arginine, isoleucine, leucine and phenylalanine. The structure of the glucan was analyzed on the basis of total acid hydrolysis, methylation, periodate oxidation and NMR studies (1H, 13C, TOCSY, DQF-COSY, NOESY, and HMQC), and the repeating unit of the glucan is established as: --> 6)-alpha-D-Glcp-(1-->.
Subject(s)
Glucans/chemistry , Termitomyces/chemistry , Carbohydrate Conformation , Glucans/isolation & purification , Nuclear Magnetic Resonance, Biomolecular , SolubilityABSTRACT
A water-soluble polysaccharide, isolated from fruiting bodies of Lagenaria siceraria, is composed of methyl-alpha-d-galacturonate, 3-O-acetyl methyl-alpha-d-galacturonate, and beta-d-galactose in a ratio of nearly 1:1:1. Compositional analysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, 2D-COSY, TOCSY, NOESY, HMQC, and HMBC) revealed the presence of the following repeating unit in the polysaccharide: [carbohydrate structure: see text] This polysaccharide showed cytotoxic activity in vitro against human breast adenocarcinoma cell line (MCF-7).
Subject(s)
Cucurbitaceae/chemistry , Fruit/chemistry , Polysaccharides/chemistry , Polysaccharides/pharmacology , Carbohydrate Sequence , Cell Line, Tumor , Cell Survival/drug effects , Humans , Magnetic Resonance Spectroscopy , Molecular Sequence Data , Polysaccharides/isolation & purificationABSTRACT
A polysaccharide (Fr. II) has been isolated from the hot aqueous extract of the fruit bodies of an edible mushroom, Volvariella diplasia. The polysaccharide contains glucose only. On the basis of total acid hydrolysis, methylation analysis, NMR studies ((1)H, (13)C, 2D-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC), and MALDI-TOFMS analysis, the structure of the repeating unit of the polysaccharide was established as formula see text.
Subject(s)
Glucans/analysis , Volvariella/chemistry , Carbohydrate Sequence , Magnetic Resonance Spectroscopy/standards , Molecular Sequence Data , Molecular Weight , Reference Standards , Solubility , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Water/chemistryABSTRACT
A water-soluble polysaccharide, isolated from the hot aqueous extract of an edible mushroom, Volvariella bombycina, consists of d-glucose, d-mannose, and d-galactose in a molar ratio 2:1:1. On the basis of total hydrolysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC), and MALDI-TOFMS analysis, the repeating unit of the polysaccharide is established as [table in text].
Subject(s)
Agaricales/chemistry , Magnetic Resonance Spectroscopy/methods , Polysaccharides/chemistry , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , Agaricales/metabolism , Carbohydrate Sequence , Hydrolysis , Methylation , Models, Chemical , Molecular Sequence Data , Monosaccharides/chemistry , Oxygen/chemistry , Periodic Acid/chemistry , Polysaccharides/analysis , Temperature , Water/chemistryABSTRACT
A heteropolysaccharide isolated from the gum (Katira) of Cochlospermum religiosum was found to consist of D-galactose, D-galacturonic acid and L-rhamnose in a molar ratio 2:1:3. Structural assignment of the polysaccharide was carried out using total acid hydrolysis, methylation analysis, periodate oxidation, Smith degradation and NMR studies (1H, 13C, DQF-COSY, TOCSY, NOESY, HMBC and HSQC) and the repeating unit of the polysaccharide was established as [Formula: see text].
Subject(s)
Bixaceae/chemistry , Polysaccharides/chemistry , Polysaccharides/isolation & purification , Carbohydrate Sequence , Galactose/chemistry , Hexuronic Acids/chemistry , Hydrolysis , Methylation , Molecular Sequence Data , Nuclear Magnetic Resonance, Biomolecular , Oxidation-Reduction , Periodic Acid/chemistry , Rhamnose/chemistryABSTRACT
A water-soluble polysaccharide, (Fr. I) isolated from the aqueous extract of an edible mushroom, Volvariella diplasia, is composed of D-glucose, D-mannose, and D-galactose in a molar ratio 3:1:1. Compositional analysis, methylation analysis, periodate oxidation study, Smith degradation, and NMR studies (1H, 13C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) revealed the presence of the following repeating unit in the polysaccharide: [Formula: see text].
Subject(s)
Agaricales/chemistry , Polysaccharides/chemistry , Polysaccharides/isolation & purification , Carbohydrate Sequence , Galactose/chemistry , Glucose/chemistry , Magnetic Resonance Spectroscopy , Mannose/chemistry , Methylation , Molecular Sequence Data , Oxidation-Reduction , Periodic Acid , SolubilityABSTRACT
A water-soluble fucoglucan, isolated from alkali-treated edible mushroom, Termitomyces robustus, consists of L-fucose and D-glucose in a molar proportion of 1:4. Structural investigation of the polysaccharide was carried out by using total hydrolysis, methylation analysis, and periodate oxidation followed by GLC-MS, and the final structure was determined using NMR experiments (1H, 13C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of the above experiments it is concluded that the following repeating unit is present in the polysaccharide: [Formula: see text].
Subject(s)
Agaricales/chemistry , Fucose/chemistry , Glucans/analysis , Polysaccharides/chemistry , Polysaccharides/isolation & purification , Carbohydrate Sequence , Hydrolysis , Magnetic Resonance Spectroscopy , Methylation , Molecular Sequence Data , Oxidation-ReductionABSTRACT
A water-soluble polysaccharide was isolated from an alkaline extract of the fruits of the ectomycorrhizal fungus, Astraeus hygrometricus. It was found to contain D-mannose, D-glucose, and L-fucose in a molar ratio of 1:2:1. On the basis of total hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and NMR studies (1H, 13C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) the structure of the repeating unit of the polysaccharide was established as [Formula: see text]
Subject(s)
Basidiomycota/chemistry , Fruiting Bodies, Fungal/chemistry , Polysaccharides/chemistry , Polysaccharides/isolation & purification , Carbohydrate Sequence , Magnetic Resonance Spectroscopy , Molecular Sequence DataABSTRACT
A water-soluble polysaccharide was isolated from the aqueous extract of the stem of Lagenaria siceraria. The polysaccharide was found to be constituted of methyl d-galacturonate, 2-O-methyl-D-xylose, and d-xylose in a ratio of 1:1:1. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, NMR studies ((1)H, (13)C, 2D-COSY, TOCSY, NOESY, HSQC, and HMBC), and MALDI-TOF MS analysis, the structure of the repeating unit of the polysaccharide is determined as.
Subject(s)
Plants/chemistry , Polysaccharides/chemistry , Carbohydrate Sequence , Magnetic Resonance Spectroscopy , Molecular Sequence Data , Plant Extracts , Solubility , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , WaterABSTRACT
A water-soluble glucan was isolated from an edible mushroom, Termitomyces microcarpus. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation and NMR studies ((1)H, (13)C, TOCSY, DQF-COSY, NOESY and HSQC), the repeating unit of the polysaccharide is established as -->4)-alpha--Glcp-(1-->3)-beta--Glcp-(1-->
Subject(s)
Agaricales/chemistry , Glucans/chemistry , Glucans/isolation & purification , Water/chemistry , Gas Chromatography-Mass Spectrometry , Magnetic Resonance Spectroscopy , SolubilityABSTRACT
A water-soluble polysaccharide was isolated from the aqueous extract of pods of Moringa oleifera. The polysaccharide contains d-galactose, 6-O-Me-D-galactose, D-galacturonic acid, l-arabinose, and l-rhamnose in a molar ratio of 1:1:1:1:1. On the basis of total hydrolysis, methylation analysis, periodate oxidation, and NMR ((1)H, (13)C, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, and HMBC) studies, the repeating unit of the polysaccharide is established as [structure: see text].
