ABSTRACT
The direct methods for diagnosis of bovine brucellosis have several limitations, therefore serological tests are the basis for the diagnosis of the disease. However, a meta-analysis estimating the diagnostic sensitivity (DSe) and diagnostic specificity (DSp) on the main tests used in bovine brucellosis control programs worldwide has not been performed. This systematic review and meta-analysis aimed to estimate the DSe, DSp and thereby accuracy of serological tests individually used in the diagnosis of bovine brucellosis. The databases CABI, Cochrane Library, PubMed/MEDLINE, SciELO, Scopus and Web of Science were used to select articles. The search resulted in 5308 studies, of which 71 were selected for systematic review using quality assessment tools and 65 studies were included in the meta-analysis. For the meta-analysis, 178 assays and 11 different serological tests were considered. To estimate DSe and DSp of the tests, studies were divided according to animal selection for the studies: (1) studies that carried out a random or consecutive selection of participants (noncasecontrol studies) and (2) all studies, including casecontrol studies. Considering only the non-case-control studies to estimate the DSe, the tests that exhibited the best and worst performance were the iELISA test (indirect enzyme immunoassay - bacterial suspension as antigen - BS) (96.5%, 95% CI: 94.1-97.9%) and 2ME (2- mercaptoethanol test) (85.0%, 95% CI: 79.6-89.1%), respectively; while for DSp, the FPA (fluorescence polarization assay) (99, 7%, 95% CI: 99.5-99.8%) and PCFIA tests (protein concentration fluorescence immunoassay) (78.5%, 95% CI: 70.0-85.1%) showed better and worse performance, respectively. Overall, our results showed an overestimation in the DSe and DSp of the eleven serological tests assessed when casecontrol studies were included in the meta-analysis, which is a concern considering its impacts on the time and costs associated with populational diagnosis of the diseases, since several of these tests are routinely used in the control and eradication programs of bovine brucellosis worldwide. Furthermore, the tests that exhibited the best DSe and DSp, iELISA (BS) and FPA, respectively, are relatively easy to perform and interpret and the test which showed the best overall accuracy was FPA.
Subject(s)
Brucellosis, Bovine , Brucellosis , Cattle Diseases , Cattle , Animals , Sensitivity and Specificity , Brucellosis, Bovine/diagnosis , Fluorescence Polarization Immunoassay/methods , Fluorescence Polarization Immunoassay/veterinary , Enzyme-Linked Immunosorbent Assay/veterinary , Enzyme-Linked Immunosorbent Assay/methods , Serologic Tests/veterinary , Brucellosis/diagnosis , Brucellosis/veterinary , Antibodies, BacterialABSTRACT
OBJECTIVE: To compare the major outcomes of use of metformin and glyburide in treatment of gestational diabetes mellitus. METHODS: Studies published in English, in the last 10 years, in the databases MEDLINE®, SciELO, LILACS and Cochrane Library were analyzed, and randomized controlled trials were selected. Health Sciences Descriptors were used to compose the search phrase, and the keywords "Gestational diabetes", "Glyburide", "Metformin" and their variations were searched in the Medical Subject Headings. PRISMA systematization was used to prepare this review, and a meta-analysis was conducted aiming to mathematically show the results of fasting blood glucose, postprandial blood glucose, birth weight and weight gain during pregnancy after using metformin and glyburide. RESULTS: The studies evaluated birth weight, neonatal hypoglycemia, mode of delivery, need for intensive care, Apgar score, macrosomia, fasting glucose, postprandial glucose and weight gain during pregnancy. In 60% of studies, there were no statistically significant differences regarding safety and efficacy of administration of metformin and glyburide. Meta-analysis demonstrated the absence of statistical differences between these drugs in fasting blood glucose (p=0.821), postprandial blood glucose (p=0.217) and birth weight (p=0.194). However, significant differences were shown in weight gain during pregnancy (p=0.036). CONCLUSION: The methods are effective, but the adverse effects of glyburide are more common; therefore, the use of metformin should be recommended, if in monotherapy.
Subject(s)
Diabetes, Gestational , Metformin , Blood Glucose , Diabetes, Gestational/drug therapy , Female , Glyburide/adverse effects , Glyburide/therapeutic use , Humans , Hypoglycemic Agents/therapeutic use , Infant, Newborn , Insulin/therapeutic use , Metformin/adverse effects , Metformin/therapeutic use , PregnancyABSTRACT
ABSTRACT Objective To compare the major outcomes of use of metformin and glyburide in treatment of gestational diabetes mellitus. Methods Studies published in English, in the last 10 years, in the databases MEDLINE®, SciELO, LILACS and Cochrane Library were analyzed, and randomized controlled trials were selected. Health Sciences Descriptors were used to compose the search phrase, and the keywords "Gestational diabetes", "Glyburide", "Metformin" and their variations were searched in the Medical Subject Headings. PRISMA systematization was used to prepare this review, and a meta-analysis was conducted aiming to mathematically show the results of fasting blood glucose, postprandial blood glucose, birth weight and weight gain during pregnancy after using metformin and glyburide. Results The studies evaluated birth weight, neonatal hypoglycemia, mode of delivery, need for intensive care, Apgar score, macrosomia, fasting glucose, postprandial glucose and weight gain during pregnancy. In 60% of studies, there were no statistically significant differences regarding safety and efficacy of administration of metformin and glyburide. Meta-analysis demonstrated the absence of statistical differences between these drugs in fasting blood glucose (p=0.821), postprandial blood glucose (p=0.217) and birth weight (p=0.194). However, significant differences were shown in weight gain during pregnancy (p=0.036). Conclusion The methods are effective, but the adverse effects of glyburide are more common; therefore, the use of metformin should be recommended, if in monotherapy.
Subject(s)
Humans , Female , Pregnancy , Diabetes, Gestational/drug therapy , Metformin/adverse effects , Metformin/therapeutic use , Blood Glucose , Glyburide/adverse effects , Glyburide/therapeutic use , Hypoglycemic Agents/therapeutic use , Insulin/therapeutic useABSTRACT
As infecções parasitárias intestinais são amplamente distribuídas em todo o mundo e a população infantil é a mais afetada. A prevalência das infecções parasitárias intestinais materna e infantil de pacientes atendidos em uma unidade de saúde pública do município de Lagoa Santa, Minas Gerais foi avaliada pela técnica de centrífugo sedimentação em formol-éter. A positividade foi de 41,7% para gestantes, 37,3% para mães e 35,7% em crianças. Endolimax nana (17,1%) e Giardia intestinalis (17,1%) foram os parasitos mais frequentemente detectados em crianças com idade inferior a dois anos e em gestantes (33,3%). Para as mães, G. intestinalis (20,3%) foi o parasito mais prevalente seguido de Entamoeba coli (17%). A infecção simultânea de mãe e filho foi detectada em 7 das 25 crianças infectadas. Apesar das ações de educação em saúde, melhoria no nível educacional e acesso a serviços médicos, a frequência de infecções por endoparasitas permanece alta. A literatura é escassa em relação ao tema e acredita-se que a deficiência na aplicação de medidas de educação sanitária possa fazer com que mães infectadas possam contribuir para a infecção parasitária de seus filhos, assim como os filhos podem ser fonte de infecção para suas mães.
Intestinal parasitic infections are widely distributed throughout the world and children are the most affected population. The objective of the present study was to evaluate the prevalence of parasitic maternal and infant intestinal infection of patients attended at a public health unit in the municipality of Lagoa Santa, Minas Gerais. The centrifugal-sedimentation in formol-ether was the technica used for stool analysis. Positivity was observed in 41.7% for pregnant women, 37.3% for mothers and 35.7% for children. Endolimax nana (17.1%) and Giardia intestinalis (17.1%) were the most frequently detected parasites in children under two-years old and pregnant women (33.3%). For mothers, G. intestinalis (20.3%) was the most prevalent parasite followed by Entamoeba coli (17%). Simultaneous infection of mother and child was detected in 7 of 25 infected children. Despite health educations actions, improvement in educational level, and access to medical services, the frequency of endoparasite infections remains high. Literature is scarce in relation to the present theme and it is believed that infected mothers can contribute for parasitic infection of their kids, just as children can be a source of infection for their mothers.
Subject(s)
Female , Humans , Pregnancy , Infant, Newborn , Parasitic Diseases/epidemiology , Protozoan Infections/epidemiology , Intestines/parasitology , Brazil , Endolimax , Entamoeba , Giardia lamblia , Public HealthABSTRACT
As infecções parasitárias intestinais são amplamente distribuídas em todo o mundo e a população infantil é a mais afetada. A prevalência das infecções parasitárias intestinais materna e infantil de pacientes atendidos em uma unidade de saúde pública do município de Lagoa Santa, Minas Gerais foi avaliada pela técnica de centrífugo sedimentação em formol-éter. A positividade foi de 41,7% para gestantes, 37,3% para mães e 35,7% em crianças. Endolimax nana (17,1%) e Giardia intestinalis(17,1%) foram os parasitos mais frequentemente detectados em crianças com idade inferior a dois anos e em gestantes (33,3%). Para as mães, G. intestinalis (20,3%) foi o parasito mais prevalente seguido de Entamoeba coli (17%). A infecção simultânea de mãe e filho foi detectada em 7 das 25 crianças infectadas. Apesar das ações de educação em saúde, melhoria no nível educacional e acesso a serviços médicos, a frequência de infecções por endoparasitas permanece alta. A literatura é escassa em relação ao tema e acredita-se que a deficiência na aplicação de medidas de educação sanitária possa fazer com que mães infectadas possam contribuir para a infecção parasitária de seus filhos, assim como os filhos podem ser fonte de infecção para suas mães. (AU)
Intestinal parasitic infections are widely distributed throughout the world and children are the most affected population. The objective of the present study was to evaluate the prevalence of parasitic maternal and infant intestinal infection of patients attended at a public health unit in the municipality of Lagoa Santa, Minas Gerais. The centrifugal-sedimentation in formol-ether was the technica used for stool analysis. Positivity was observed in 41.7% for pregnant women, 37.3% for mothers and 35.7% for children. Endolimax nana (17.1%) and Giardia intestinalis(17.1%) were the most frequently detected parasites in children under two-years old and pregnant women (33.3%). For mothers, G. intestinalis (20.3%) was the most prevalent parasite followed by Entamoeba coli (17%). Simultaneous infection of mother and child was detected in 7 of 25 infected children. Despite health educations actions, improvement in educational level, and access to medical services, the frequency of endoparasite infections remains high. Literature is scarce in relation to the present theme and it is believed that infected mothers can contribute for parasitic infection of their kids, just as children can be a source of infection for their mothers. (AU)
