Prevalence of condylar morphological changes in individuals with class II malocclusion.

This observational, cross-sectional study with retrospective collection was aimed to evaluate the prevalence of morphological changes in mandibular condyles in individuals with class II malocclusion, classified according to different vertical growth patterns (brachyfacial, mesofacial, and dolichofacial), through cone beam computed tomography (CBCT). Seventy CBCT images (140 mandibular condyles) were selected from individuals without orthodontic treatment, of both sexes, aged between 25 and 50 years. No statistically significant differences were found between facial patterns; however, there was a higher relative prevalence of bone changes in dolichofacial individuals with flattening (62%), sclerosis (44%), and subchondral bone cyst (20%). Erosion and osteophytes prevailed in mesofacial (39%), and brachyfacial individuals (32%), respectively. Thus, there was no statistically significant difference in the prevalence of degenerative changes between the vertical skeletal patterns. Flattening was the most prevalent change, whereas subchondral bone cyst was the least prevalent among the three groups studied. The observational design of this study makes it possible to analyze image banks to verify the correlation of morphological changes in the temporomandibular joint in different facial patterns in patients with class II malocclusion. A limitation of the study is that clinical characteristics were not evaluated.

Genomic studies of the additive and dominant genetic control on production traits of Euterpe edulis fruits.

In forest genetic improvement programs for non-domesticated species, limited knowledge of kinship can compromise or make the estimation of variance components and genetic parameters of traits of interest unfeasible. We used mixed models and genomics (in the latter, considering additive and non-additive effects) to evaluate the genetic architecture of 12 traits in juçaizeiro for fruit production. A population of 275 genotypes without genetic relationship knowledge was phenotyped over three years and genotyped by whole genome SNP markers. We have verified superiority in the quality of the fits, the prediction accuracy for unbalanced data, and the possibility of unfolding the genetic effects into their additive and non-additive terms in the genomic models. Estimates of the variance components and genetic parameters obtained by the additive models may be overestimated since, when considering the dominance effect in the model, there are substantial reductions in them. The number of bunches, fresh fruit mass of bunch, rachis length, fresh mass of 25 fruits, and amount of pulp were strongly influenced by the dominance effect, showing that genomic models with such effect should be considered for these traits, which may result in selective improvements by being able to return more accurate genomic breeding values. The present study reveals the additive and non-additive genetic control of the evaluated traits and highlights the importance of genomic information-based approaches for populations without knowledge of kinship and experimental design. Our findings underscore the critical role of genomic data in elucidating the genetic control architecture of quantitative traits, thereby providing crucial insights for driving species' genetic improvement.

Microbiological evaluation in invisible aligner chemical cleaning methods against Candida albicans and Streptococcus mutans.

INTRODUCTION: This study aimed to assess the efficacy of chemical agents in removing Candida albicans and Streptococcus mutans biofilm from invisible aligners. METHODS: The samples were made of EX30 Invisalign trays, biofilm was cultured by standardized suspensions of C. albicans ATCC strain and S. mutans clinical strain on the sample. The treatments used were 0.5% sodium hypochlorite (NaClO) (20 minutes), 1% NaClO (10 minutes), chlorhexidine (5 minutes), peroxide (15 minutes), and orthophosphoric acid (15 seconds). The control group received phosphate-buffered saline for 10 minutes. The colony-forming units per milliliter of each microorganism were determined by serial dilutions seeded in plates with selective culture mediums for each one. Data were analyzed by the Kruskal-Wallis and Conover-Iman tests at an α of 0.05. RESULTS: For the C. albicans biofilm group, the control group had 9.7 Log10 of microorganism growth, and all treatment groups had statistically significant biofilm reduction, in which chlorhexidine presented the highest inhibition of 3 Log10, followed by alkaline peroxide and orthophosphoric acid both with 2.6 Log10, 1% NaClO (2.5 Log10), and 0.5% NaClO (2 Log10). As for S. mutans, the control group had 8.9 Log10 of growth, and a total microorganism inhibition was reached by chlorhexidine, 1% NaClO, and orthophosphoric acid, whereas alkaline peroxide inhibited growth to 7.9 Log10 and 0.5% NaClO 5.1 Log10. CONCLUSIONS: Within the limitations, chlorhexidine and orthophosphoric acid had greater efficacy in both biofilms. In addition, 1% NaClO and alkaline peroxide also had significant effects; therefore, their incorporation aligners disinfection protocols are valid.

OpenCare5G: O-RAN in Private Network for Digital Health Applications.

Digital Health is a new way for medicine to work together with computer engineering and ICT to carry out tests and obtain reliable information about the health status of citizens in the most remote places in Brazil in near-real time, applying new technologies and digital tools in the process. InovaHC is the technological innovation core of the Clinics Hospital of the Faculty of Medicine of the University of São Paulo (HCFMUSP). It is the first national medical institution to seek new opportunities offered by 5G technology and test its application in the first private network for Digital Health in the largest hospital complex in Latin America through the OpenCare5G Project. This project uses an Open RAN concept and network disaggregation with lower costs than the traditional concept used by the telecommunications industry. The technological project connected to the 5G network was divided into two phases for proof-of-concept testing: the first with an initial focus on carrying out examinations with portable ultrasound equipment in different locations at HCFMUSP, and the second focusing on carrying out remote examinations with health professionals in other states of Brazil, who will be working in remote areas in other states with little or no ICT infrastructure together with a doctor analyzing exams in real time at HCFMUSP in São Paulo. The objective of the project is to evaluate the connectivity and capacity of the 5G private network in these the proof-of-concept tests for transmitting the volume of data from remote exams with higher speed and lower latency. We are in the first phase of the proof of concept testing to achieve the expected success. This project is a catalyst for innovation in health, connecting resources and entrepreneurs to generate solutions for the innovation ecosystem of organizations. It is coordinated by Deloitte with the participation of the Escola Politécnica da USP (The School of Engineering-University of São Paulo), Airspan, Itaú Bank, Siemens Healthineers, NEC, Telecom Infra Projet, ABDI and IDB. The use of 5G Open RAN technology in public health is concluded to be of extreme social, economic, and fundamental importance for HCFMUSP, citizens, and the development of health research to promote great positive impacts ranging from attracting investment in the country to improving the quality of patient care.

Desafios para o funcionamento dos conselhos dos direitos da pessoa idosa e seus reflexos sociais / Challenges for the operation of councils for the rights of older people and their social consequences

Resumo Objetivo compreender as percepções dos conselheiros acerca do funcionamento dos conselhos dos direitos da pessoa idosa no estado do Rio Grande do Norte. Método Trata-se de um estudo de natureza transversal analítica com abordagem quantitativa realizado com os conselheiros municipais dos direitos da pessoa idosa do estado do Rio Grande do Norte. Participaram 109 conselheiros, havendo predominância de membros titulares, do sexo feminino, de representação governamental e com nível de escolaridade superior. Resultados Foram apontados diversos desafios para atuação e funcionamento dos conselhos, dentre os quais destacam-se: ações isoladas, baixa participação de membros governamentais e representação da sociedade civil, pouco tempo de discussão nas reuniões e necessidade de instrumentos válidos para monitorar as ações dos conselhos. Evidenciaram-se também baixo conhecimento técnico dos conselheiros e aversão às divergências e posicionamentos políticos. Conclusão é imprescindível assegurar políticas intersetoriais, maior independência perante o executivo e, prioritariamente, maior capacitação dos conselheiros e fortalecimento político democrático para que possam não só promover, mas exercer um protagonismo sociopolítico a partir de uma construção coletiva e representatividade social.

Abstract Objective to understand the perceptions of councilors about the functioning of councils for the rights of older people in the state of Rio Grande do Norte. Method This is an analytical cross-sectional study with a quantitative approach carried out with municipal councilors for the rights of older people in the state of Rio Grande do Norte. 109 councilors participated, with a predominance of full members, female, from government representation and with a higher education level. Results Several challenges were identified for the performance and operation of the councils, among which stand out: isolated actions, low participation of government members and representation of civil society, little time for discussion in meetings and the need for valid instruments to monitor the actions of the councils. Low technical knowledge of the councilors and aversion to divergences and political positions were also evidenced. Conclusion it is essential to ensure intersectoral policies, greater independence from the executive and, above all, greater training of councilors and democratic political strengthening so that they can not only promote, but exercise a socio-political protagonism based on a collective construction and social representation.

Aspectos determinantes para construção social da pessoa idosa a partir das políticas públicas no Brasil / Determining aspects for the social construction of the older adult based on public policies in Brazil

Resumo Apesar do envelhecimento ser uma realidade global, há, na velhice, um cerceamento das experiências individuais com a sua subjetivação, na qual o aperfeiçoamento passa a ser uma exigência moral. Objetivamos compreender os aspectos determinantes para a construção social da pessoa idosa a partir das políticas públicas no Brasil. Realizamos uma análise documental por meio da técnica de análise de conteúdo proposta por Laurence Bardin, operacionalizada pelo software IRAMUTEQ. O corpus textual foi constituído por 13 documentos que orientam as políticas destinadas à proteção social da pessoa idosa, posteriores à Constituição Federal de 1988. Observamos que os mecanismos de poder implícitos nas políticas são subordinados aos imperativos capitalistas e à racionalidade biomédica prescritiva, voltada aos aspectos estritamente biológicos que desnaturalizam e homogeneízam o envelhecimento à luz de um ideal de juventude ininterrupta. Ancora-se na "reprivatização" da velhice, culpabilizando os idosos por suas condições de vida. Essa ideologia produtivista estabelece a moralização dos atributos físicos que definem e moldam as subjetividades de uma sociedade refratária à própria velhice. Destacamos a importância de repensar o envelhecimento com base nas problemáticas sociais, ressaltando aspectos singulares da trajetória de vida da pessoa idosa, alheia à ideologia do envelhecimento sem velhice.

Abstract Despite aging being a global reality, old age has a restriction of individual experiences with their subjectivation, in which improvement becomes a moral requirement. We aim to understand the determinant aspects for the social construction of the older adults based on public policies in Brazil. We performed a document analysis based on the content analysis technique proposed by Laurence Bardin, operationalized by the IRAMUTEQ software. The textual corpus comprised 13 documents that guide policies aimed at the social protection of older adults, posterior to the Federal Constitution of 1988. We observe that the power mechanisms implicit in policies are subordinated to capitalist imperatives and to prescriptive biomedical rationality, focused on strictly biological aspects that denaturalize and homogenize aging in the light of an ideal of uninterrupted youth. It is anchored in the "reprivatization" of old age, which blames the older adults for their living conditions. This productivist ideology establishes the moralization of physical attributes that define and shape the subjectivities of a society refractory to old age itself. We highlight the importance of rethinking aging based on social issues, highlighting singular aspects of the life trajectory of the older person. unfamiliar with the ideology of aging without old age.

Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices / Análise da formação de biofilme por Candida albicans em diferentes tipos de aparelhos e dispositivos ortodônticos fixos

Objective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018"x.025" showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms (AU)

Objetivo: neste estudo, a formação de biofilme por Candida albicans em aparelhos ortodônticos fixos foi avaliada. Material e Métodos: um total de 300 bráquetes metálicos convencionais (MC), cerâmicos (CB), autoligados (SLB), com fios de níquel-titânio (NiTi) e níquel-cromo (NiCr) e tipos de ligaduras foram organizados em trinta grupos (n=10). Bráquetes, fios e ligaduras foram unidos, esterilizados, colocados em placas de 24 poços, contaminados com suspensões padronizadas de C. albicans (107 células/mL) e incubados a 37°C por 48 h para a formação de biofilmes. Os biofilmes formados foram rompidos por meio de um homogeneizador ultrassônico e suspensões foram diluídas e semeadas em ágar Sabouraud-dextrose para determinar as unidades formadoras de colônias por mL. A microscopia eletrônica de varredura foi realizada antes e após a formação do biofilme. Resultados: foi observada menor formação de biofilme no grupo MC em comparação aos grupos CB e SLB (p<0,0001). A formação de biofilme foi semelhante nos grupos SLB e CB (p=0,855). Em geral, os fios de seção transversal .018"x.025" apresentaram maior formação de biofilme quando associados aos três tipos de bráquetes. Os conjuntos com fios de NiCr e ligaduras SSL com bráquetes MC (p=0,0008) e CB (p=0,0003) apresentaram maior formação de biofilme. Conclusão: bráquetes MC com fios de NiTi e NiCr apresentaram menor formação de biofilme, independente da ligadura e secção transversal ou bitola do fio e, braquetes MC e CB com fios de NiCr e ligaduras SSL foram mais propensos a acumular biofilmes.(AU)

Systemic scleroderma: imaging findings of diagnosis and clinical management of temporomandibular joint disorders / Esclerodermia sistêmica: diagnóstico por achados imaginológicos e manejo clínico das disfunções temporomandibulares

Scleroderma, an autoimmune disease, directly affects the production of collagen in the connective tissue. In its systemic form, the disease causes oral manifestations such as: limited mouth opening, xerostomia, periodontal disease, thickening of the periodontal ligament and bone resorption of the mandible. This case report aims to draw attention to the difficulties encountered in providing dental care to patients with scleroderma and also to highlight the imaging findings, with emphasis on the temporomandibular joints, which are of interest to dentists about the disease. In the present case, the patient presented bilateral condylar erosion, in addition to disc displacement without reduction. Due to the systemic condition of the patient, it was decided to make an individualized occlusal splint. The limitation of mouth opening is a limiting factor for the manufacture of prostheses and plates, which is why partial prostheses are indicated and are easily removed by the patient. The decisions taken have a great impact on the health and quality of life of patients in these conditions, so there is a need for multidisciplinary involvement in order to arrive at the best treatment plan. After five years of using the stabilizing plate overnight, the patient reports greater comfort and muscle relaxation upon waking up (AU)

Esclerodermia, uma doença autoimune, afeta diretamente a produção de colágeno do tecido conjuntivo. Na forma sistêmica, a doença causa manifestações bucais, como: limitação de abertura bucal, xerostomia, doença periodontal, espessamento do ligamento periodontal e reabsorção da mandíbula. Este relato de caso tem por objetivo chamar atenção para as dificuldades encontradas ao promover atendimento odontológico para pacientes com esclerodermia e também destacar os achados imaginológicos, com ênfase na articulação temporomandibular, que são da doença e de interessa ao cirurgião-dentista. No presente caso, a paciente apresentava erosão condilar bilateral, com deslocamento de disco sem redução. Devido à condição sistêmica da paciente, foi decidido confeccionar uma placa oclusal individualizada. A limitação de abertura bucal é um fator limitante para confecção de próteses e placas, por isso próteses parciais são indicadas, além de serem de fácil remoção pelo paciente. As decisões tomadas tem grande impacto na saúde e qualidade de vida de pacientes nessas condições, deste modo é necessário uma equipe multidisciplinar envolvidas para chegar no melhor plano de tratamento. Após cinco anos fazendo uso da placa estabilizadora durante a noite, a paciente relata maior conforto e relaxamento muscular ao acordar (AU)

Impact of bruxism on craniomandibular morphology: A cone-beam computed tomographic study.

OBJECTIVE: The main goal of this work was to address craniomorphological characteristics of patients with bruxism when compared to those without bruxism using cone beam computed tomography (CBCT) imaging. METHODS: Seventy CBCT images of an equal number (n = 35) of orthodontic patients with and without bruxism (age range, 18-44 years) were retrospectively analyzed. Sagittal evaluation, mandibular shape, and skeletal asymmetry were systematically assessed in both groups. RESULTS: Significant differences (p < 0.05) were observed between groups for Right Articular Fossa (AF) - Axial Plane (AP) and Left AF - AP (B > NB), Right Gonial Angle (GA), Left GA, Sella-Nasion and Occlusal Plane (B < NB). Age (13%), Condyle-Gonion (18%), AF to AP (67%), and Mental-AP (16%) had the greatest impact on bruxers. CONCLUSION: In this study, the CBCT 3D image showed significant differences in craniofacial morphology, particularly in the mandibular structure of bruxers compared with non-bruxers.

Down syndrome: orofacial pain, masticatory muscle hypotonia, and sleep disorders.

The aim of the present study was to investigate orofacial pain in individuals with Down syndrome (DS) and determine possible associations with masticatory muscle hypotonia (MMH), maximum mouth opening (MMO), and sleep disorders. Twenty-three individuals with DS underwent a standardized clinical examination using Axis I of the Diagnostic Criteria for Temporomandibular Disorders, for the diagnosis of pain in the masseter and temporal muscles and temporomandibular joint (TMJ). MMH was investigated using electromyography of the temporal and masseter muscles and the measurement of maximum bite force (MBF). MMO was measured using an analog caliper. Sleep disorders (obstructive sleep apnea [OSA], snoring index [SI], and sleep bruxism index [SBI]) were investigated using type II polysomnography. Statistical analysis was performed. Nonsignificant differences were found in muscle and TMJ pain between the sexes. However, myalgia and referred myofascial pain in the left masseter muscle were more frequent in males (69%) than females (40%). Electrical activity of the temporal (left: p = .002; right: p = .004) and masseter (left: p = .008) muscles was significantly lower in males than in females. MBF range was lower in males than females, indicating the highest MMH among males. OSA, SI, and SBI were identified in both sexes, but with no statistically significant differences. We concluded that myalgia and referred myofascial pain were found in some individuals with DS, especially in males. Arthralgia was found mainly in females. Temporal and masseter myalgia may have exerted an influence on the severity of MMH in males, particularly on the left side.

Psychological Factors in Temporomandibular Disorders Patients during COVID-19 Pandemic: A Cross-Sectional Study

Abstract Objective: To assess the influence of the COVID-19 pandemic on levels of anxiety, stress, and orofacial pain in individuals with and without TMD. Material and Methods: In this cross-sectional study, the participants were divided into two groups (n=10, each) experimental group (EG) TMD patients and control group (CG), without TMD. The presence of TMD was confirmed using the RDC/TMD. The Beck Anxiety Scale (BAI), Perceived Stress Scale (PSS), and Oral Analog Scale were used for assessment. The Beck Anxiety Scale is composed of 21 multiple-choice questions describing symptoms of anxiety, while the PSS is a measure that evaluates life situations as stressful. An Oral Analogue Scale was applied to each individual to quantify orofacial pain, with 0 (zero) without pain and 10 (ten) being the worst pain ever felt. The comparison between groups was performed using the Mann-Whitney test. Logistic regression was used to verify TMD dependence in relation to BAI and PSS-10. Quantitative variables were correlated with each other by Pearson's correlation coefficient. The level of significance considered for all tests was 5%. Results: The association was statistically significant (p≤0.05) for BAI and PSS with a TMD. Conclusion: Although the COVID 19 pandemic has had a psychological impact on the general population, patients with TMD have higher levels of anxiety and stress than the control group.

Evaluation of the action of LPSF/GQ-16 on cytokines and PPAR-γ gene expression after in vitro irradiation of peripheral blood mononuclear cells.

PURPOSE: Patients submitted to radiotherapy (RT) may present in their healthy tissues surrounding the treated tumor, some typical acute inflammatory reactions induced by ionizing radiation (IR). The manifestation of inflammatory processes is a result of exacerbation of the immune system, as a response to radiation exposure, and this can be a limiting factor for RT protocols. To counteract this, some thiazolidinediones, such as LPSF/GQ-16, may be useful for modulating the patient's radioinduced inflammatory response in normal tissues. In this context, the present work aims to evaluate the activity of LPSF/GQ-16 on the levels of cytokines and the expression of the gene PPARγ in mononuclear cells irradiated in vitro, to analyze the immunomodulatory activity of the molecule and its action on radiomitigation. MATERIALS AND METHODS: For this, blood samples from eight donors were collected and irradiated with 2 Gy, then the PBMC (peripheral blood mononuclear cells) were cultured and treated with LPSF/GQ-16. The levels of cytokines TNF-α, IFN-γ, IL-2 and IL-4 were quantified by CBA, while the genes of TNF-α, IFN-γ and PPARγ were analyzed by RT-PCR. RESULTS: LPSF/GQ-16 significantly reduced the expression of proinflammatory cytokines (IFN-γ and TNF-α) in irradiated and nonirradiated groups. There was no significant reduction of anti-inflammatory cytokines (IL-2 and IL-4) by LPSF/GQ-16. The mRNA expression of PPAR-γ, IFN-γ and TNF-α in the presence of LPSF/GQ-16 was higher in the nonirradiated sample. CONCLUSION: LPSF/GQ-16 showed effective activity after irradiation, with an important immunomodulatory activity in irradiated PBMCs.

'Candidatus Mycoplasma haemoalbiventris' and tick-borne pathogens screening in white-eared opossums (Didelphis albiventris) from Curitiba and Foz do Iguaçu Cities, Paraná State, southern Brazil.

Hemoplasmas are epierythrocytic bacteria that infect mammals. 'Candidatus Mycoplasma haemoalbiventris' was detected in white-eared opossums (Didelphis albiventris) from southern and central-western Brazil. The present study aimed at: i) screening opossums for tick-borne (TBP) pathogens (Piroplasmida and Anaplasmataceae) and ii) detecting and characterizing hemoplasma species infecting opossums from Curitiba and Foz do Iguaçu cities in the Paraná State, southern Brazil. Thirty blood samples from white-eared opossums were evaluated by PCR assays. Animals were not infested by ectoparasites. The mammalian endogenous gapdh gene was consistently amplified in all samples. All opossums tested negative for Theileria/Babesia spp. and Ehrlichia/Anaplasma spp. by PCR based on 18S rRNA and 16S rRNA genes, respectively. A genus-specific PCR assay based on the 16S rRNA gene of hemoplasmas showed that three/13 (23.08%; CI 95%: 8.18-50.26%) opossums from Foz do Iguaçu were positive for hemotropic Mycoplasma sp. All opossums from Curitiba tested negative for hemoplasmas. Sequencing of both the 16S and 23S rRNA genes revealed that the animals were infected by 'Ca. M. haemoalbiventris'. Although 'Ca. M. haemoalbiventris' is prevalent in opossums in Brazil, clinical signs associated with its infection and its putative vectors remain unknown.

'Candidatus Mycoplasma haemoalbiventris' and tick-borne pathogens screening in white-eared opossums (Didelphis albiventris) from Curitiba and Foz do Iguaçu Cities, Paraná State, southern Brazil / 'Candidatus Mycoplasma haemoalbiventris' e triagem para patógenos transmitidos por carrapatos em gambás de orelha branca (Didelphis albiventris) das cidades de Curitiba e Foz do Iguaçu, Estado do Paraná, sul do Brasil

Abstract Hemoplasmas are epierythrocytic bacteria that infect mammals. 'Candidatus Mycoplasma haemoalbiventris' was detected in white-eared opossums (Didelphis albiventris) from southern and central-western Brazil. The present study aimed at: i) screening opossums for tick-borne (TBP) pathogens (Piroplasmida and Anaplasmataceae) and ii) detecting and characterizing hemoplasma species infecting opossums from Curitiba and Foz do Iguaçu cities in the Paraná State, southern Brazil. Thirty blood samples from white-eared opossums were evaluated by PCR assays. Animals were not infested by ectoparasites. The mammalian endogenous gapdh gene was consistently amplified in all samples. All opossums tested negative for Theileria/Babesia spp. and Ehrlichia/Anaplasma spp. by PCR based on 18S rRNA and 16S rRNA genes, respectively. A genus-specific PCR assay based on the 16S rRNA gene of hemoplasmas showed that three/13 (23.08%; CI 95%: 8.18-50.26%) opossums from Foz do Iguaçu were positive for hemotropic Mycoplasma sp. All opossums from Curitiba tested negative for hemoplasmas. Sequencing of both the 16S and 23S rRNA genes revealed that the animals were infected by 'Ca. M. haemoalbiventris'. Although 'Ca. M. haemoalbiventris' is prevalent in opossums in Brazil, clinical signs associated with its infection and its putative vectors remain unknown.

Resumo Hemoplasmas são bactérias epieritrocíticas que infectam mamíferos. 'Candidatus Mycoplasma haemoalbiventris' foi detectado previamente em gambás-de-orelha-branca (Didelphis albiventris) das regiões sul e centro-oeste do Brasil. O presente estudo objetivou: i) triar os gambás para as doenças transmitidas por carrapatos (Piroplasmida e Anaplasmataceae); e ii) detectar e caracterizar as espécies de hemoplasma que infectam gambás nas cidades de Curitiba e Foz do Iguaçu, no Estado do Paraná, sul do Brasil. Trinta amostras de sangue de gambás-de-orelha-branca foram analisadas por PCR. Os animais não estavam infestados por ectoparasitos. O gene endógeno de mamífero gapdh foi amplificado em todas as amostras. Todos os gambás testaram negativos para Theileria/Babesia spp. e Ehrlichia/Anaplasma spp. por PCR, respectivamente, para os genes 18S rRNA e 16S rRNA. Uma PCR gene-específica, baseada no gene 16S rRNA de hemoplasmas, mostrou que três/13 (23,08%; CI 95%: 8,18-50,26%) gambás de Foz do Iguaçu foram positivos para Mycoplasma sp. hemotrópico. Todos os gambás de Curitiba testaram negativos para hemoplasmas. O sequenciamento de fragmentos dos genes 16S e 23S rRNA revelou que os animais estavam infectados pelo 'Ca. M. haemoalbiventris'. Embora 'Ca. M. haemoalbiventris' seja prevalente em gambás no Brasil, os sinais clínicos associados à infecção e os prováveis vetores permanecem desconhecidos.

Modelo conceitual de cuidado a partir do protagonismo sociopolítico do idoso na atenção primária à saúde / Conceptual model of care based on the sociopolitical protagonism of the elderly in primary health care

Mesmo diante de uma sociedade envelhecida, os idosos concorrem a um cerceamento das experiências individuais a partir da indução de uma velhice universal, na qual o prolongamento da capacidade funcional e a necessidade de justificar sua utilidade são premissas de aprovação social. Objetivo: Desenvolver um modelo conceitual de cuidado a partir do protagonismo sociopolítico do idoso na Atenção Primária à Saúde. Métodos: Trata-se de uma pesquisa qualitativa exploratória com abordagem analítica e documental, constituída por cinco etapas: (1) pesquisa documental dos fatores intervenientes ao processo de formulação das políticas públicas de proteção ao idoso. Buscamos compreender as diretrizes que estabelecem seu funcionamento e as relações de poder intrínsecas aos diferentes interesses sociopolíticos e econômicos sob a prerrogativa da biopolítica; (2) pesquisa documental para compreendermos as relações de poder que determinam a construção social do idoso a partir das Políticas Nacional de Promoção da Saúde; (3) Revisão de Escopo, seguindo as diretrizes estabelecidas pelo Joanna Briggs Institute's (JBI)´s a partir da seguinte pergunta de pesquisa: quais iniciativas relevantes para promover a qualidade na Atenção Primária à Saúde do idoso em consonância com a dimensão da qualidade Cuidado Centrado no Paciente; (4) pesquisa qualitativa a partir de dados secundários colhidos no Conselho Estadual dos Direitos da Pessoa Idosa do estado do Rio Grande do Norte para compreendermos os entraves associados ao funcionamento dos conselhos de direito dos idosos e (5) estudo qualitativo considerando as etapas pregressas para o desenvolvimento do modelo conceitual de cuidado do idoso com ênfase na dimensão da qualidade Cuidado Centrado no Paciente e Atenção Primária à Saúde. Resultados e discussão: Constatamos que os mecanismos de poder implícitos nas políticas tutelam a velhice para melhor gestão e eficiência a partir dos interesses capitalistas. Desnaturaliza-se a luz de um ideal de juventude ininterrupta materializada pelo lucro e produtividade. Assim, o ideário neoliberal a partir de uma despolitização e mensagens individualistas ignora os aspectos sociais relacionados a própria trajetória do idoso para induzir, segundo uma racionalidade biomédica prescritiva e regulatória, a utopia de alcançarmos o envelhecimento sem velhice e legitimar a desresponsabilização do Estado como promotor de saúde e cuidado do idoso. Considerações finais: Em face do exposto, construímos um modelo conceitual para o cuidado do idoso contrapondo-se aos preceitos neoliberais entorno da mercantilização da saúde, em detrimento ao protagonismo sociopolítico do idoso e, portanto, ao reconhecimento dos aspectos multidimensionais como instrumentos sociais imprescindíveis de emancipação política e promoção da saúde (AU).

Even in the face of an aging society, the elderly contribute to a restriction of individual experiences from the induction of a universal old age, in which the extension of functional capacity and the need to justify its usefulness are premises of social approval. Objective: To develop a conceptual model of care based on the sociopolitical role of the elderly in Primary Health Care. Methods: This is an exploratory qualitative research with an analytical and documental approach, consisting of five stages: (1) documental research of the intervening factors to the process of formulating public policies for the protection of the elderly. We seek to understand the guidelines that establish its functioning and the power relations intrinsic to the different socio-political and economic interests under the prerogative of biopolitics; (2) documentary research to understand the power relations that determine the social construction of the elderly based on the National Health Promotion Policies; (3) Scoping Review, following the guidelines established by the Joanna Briggs Institute's (JBI)'s based on the following research question: which initiatives are relevant to promote quality in Primary Health Care for the elderly in line with the dimension of quality Person-Centered Care? (4) qualitative research based on secondary data collected at the State Council for the Rights of the Elderly Person in the state of Rio Grande do Norte to understand the obstacles associated with the functioning of the elderly legal councils and (5) qualitative study considering the previous steps to the development of the conceptual model of care for the elderly with an emphasis on the quality dimension Patient-Centered Care and Primary Health Care. Results and discussion: We found that the mechanisms of power implicit in policies protect old age for better management and efficiency based on interests capitalists. The light of an ideal of uninterrupted youth materialized by profit and productivity is denatured. Thus, the neoliberal ideology based on depoliticization and individualistic messages ignores the social aspects related to the elderly's own trajectory to induce, according to a prescriptive and regulatory biomedical rationality, the utopia of achieving aging without old age and legitimizing the State's disaccountability as promoter of health and care for the elderly. Final considerations: In view of the above, we built a conceptual model for the care of the elderly, in opposition to neoliberal precepts surrounding the commodification of health, to the detriment of the sociopolitical role of the elderly and, therefore, the recognition of multidimensional aspects as essential social instruments of political emancipation and health promotion (AU).

Genetic diversity of Euterpe edulis martius based on fruit traits / Diversidade genética de Euterpe edulis martius com base em características de frutos

Juçara (Euterpe edulis Martius) is a palm species from the Atlantic Forest whose fruits are important as a source of food to several individuals from the fauna of the region. Despite its ecological importance, juçara is found in the list of endangered species, due to the fragmentation of the forests and the illegal extraction of the heart of palm. We aimed to evaluate the inter- and intra-populational genetic diversity of E. edulis based on fruit and seed traits in forest fragments of the Espírito Santo State in Brazil. The aim was to generate information to be used in E. edulis breeding programs, or in the delineation of more efficient management and reforestation strategies. The study was carried out in 20 forest fragments and 198 fruit plants were sampled. Positive genetic association was observed between the evaluated traits, with the longitudinal diameter of the fruit (LDF) and the seed mass (SM) greatly affecting fruit mass (FM). The existence of inter- and intra-populational genetic divergence was proved. The genetic divergence found in E. edulis suggests that there is genetic material that can be explored in breeding programs and this information may also contribute to management strategies that can increase the species genetic diversity

Juçara (Euterpe edulis Martius) é uma espécie de palmeira da Mata Atlântica, que é importante como fonte de alimento para vários indivíduos da fauna. Apesar de sua importância ecológica, juçara é encontrada na lista de espécies ameaçadas à extinção, devido à fragmentação florestal e à extração ilegal do palmito. Nós tivemos como objetivo avaliar a diversidade genética inter e intra populacional de E. edulis com base em caracteres de frutos e sementes, em fragmentos florestais do Estado do Espírito Santo no Brasil, com o objetivo de gerar informações a serem utilizadas nos programas de melhoramento de E. edulis ou na delimitação de estratégias de manejo e reflorestamento mais eficientes. O estudo foi realizado em 20 fragmentos florestais e 198 plantas frutíferas foram amostradas. Foi observada uma grande associação genética positiva entre os caracteres avaliados, com as características diâmetro longitudinal dos frutos (LDF) e massa de sementes (SM) apresentando efeitos maiores do que a massa característica da fruta (FM). A existência de divergência genética inter e intrapopulacional foi comprovada. A divergência genética encontrada neste trabalho para E. edulis sugere a presença de material genético que vale a pena ser explorado em programas de melhoramento e essa informação também pode contribuir com estratégias de manejo para aumentar a diversidade genética das espécies.

Molecular Detection of Dengue Virus in Mosquitoes as an Early Indicator to Aid in the Prevention of Human Infection in Endemic Areas.

Human cases of dengue virus based on the National Dengue Control Plan were compared with the molecular detection of the dengue virus in trapped mosquitoes, verifying the prediction and efficacy potentials of vector control between the two methodologies in a city with three endemic frontiers. Molecular detection of dengue virus in trapped mosquitoes was significantly higher than in human cases (p = 0.0435). Thus, molecular detection could be used as an early indicator to help prevent more human cases of dengue.

Os efeitos da Punica granatum L. em diferentes concentrações sobre duas linhagens celulares: estudo in vitro / The effects of Punica granatum L. at different concentrations on two cell lines: in vitro study

Introdução: A Punica granatum L. (PG), utilizada como medicamento fitoterápico, apresenta propriedades terapêuticas, anti-inflamatórias e antioxidante. Embora diversos estudos já tenham sido realizados com este fitoterápico, seus possíveis efeitos citotóxicos nos tecidos humanos ainda não são claros. Objetivo: Avaliar a citotoxicidade da PG por meio de cultura celular com duas linhagens: fibroblastos humanos de mucosa oral (FLM) e células de carcinoma epidermoide oral humano (KB). Material e método: As células foram submetidas ao teste de viabilidade celular por 24 horas nas concentrações da PG 1%, 0,50%, 0,25%, 0,125%, 0,062% e 0,031%, e aos testes de citotoxicidade celular em 4 horas, 1, 3, 5 e 7 dias, em diferentes concentrações, realizados em triplicata. Foi utilizado um controle negativo (Triton 1%) e um controle positivo sem o extrato de PG. Os dados obtidos foram submetidos à ANOVA (p < 0,05). Resultado: Foi possível observar que o extrato da PG possui efeitos inibitórios, apresentando-se maior nas células KB em relação às FLM. Os testes de citotoxicidade e viabilidade mostraram inibição e morte das células KB e FLM nas concentrações 1%, 0,50% e 0,25%. Conclusão: O efeito inibitório da PG foi dose-dependentes, mostrando-se maior nas células KB em relação às FLM.

Introduction: Punica granatum L. (PG), used as a herbal medicine, has therapeutic, anti-inflammatory and antioxidant properties, although several studies have already been carried out with this herbal medicine, its possible cytotoxic effects on human tissues are still unclear. Objective: To evaluate the cytotoxicity of PG through cell culture with two strains: human oral mucosa fibroblasts (LFM) and human oral squamous cell carcinoma (KB) cells. Material and method: The cells were submitted to the cell viability test for 24 hours in the concentrations of PG 1%, 0.50%, 0.25%, 0.125%, 0.062% and 0.031% and the cell cytotoxicity tests in 4 hours, 1, 3, 5 and 7 days in different concentrations, performed in triplicate. A negative control (Triton 1%) and a positive control without the PG extract were used. The data obtained were submitted to ANOVA (p <0.05). Result: it was possible to observe that the PG extract has inhibitory effects, being higher in KB cells in relation to LFM. The cytotoxicity and viability tests showed inhibition and death of KB and FLM cells at concentrations of 1%, 0.50% and 0.25%. Conclusion: The inhibitory effect of PG was dose dependent, showing itself to be greater in KB cells compared to LMB.

Comparative study between different groups of esthetic component of the Index of Orthodontic Treatment Need and eye tracking.

INTRODUCTION: The objective of this study was to determine how different people perceive distinct malocclusions with the use of eye tracking and to compare the results with scores from the subjective visual attractiveness scale (VAS) following 3 grades obtained from the Aesthetic Component of the Index of Orthodontic Treatment Need (AC-IOTN) scale. METHODS: Ninety participants were equally distributed into 3 groups-dentists, orthodontists, and laypeople-and underwent 1 eye-tracking session. After being positioned and calibrated, they were shown 3 dental images. The images reflected the AC-IOTN grades of 1 (close to ideal), 5 (borderline need for treatment), and 8 (definite need for treatment). During the viewing, eye-tracker data were collected for fixation points, duration, and attractiveness (according to the VAS) for each image. The variables were statistically tested with the use of 2-way analysis of variance and complete factorial model (between groups, grades, and for the interactions between them). RESULTS: Grade 1 received the highest subjective score independently from the participant group. This was in contrast to grades 5 and 8, which received similar scores, although the means of fixation points and duration were not significantly different among the grades (P > 0.05). The scores obtained from the dentist group were significantly higher than those from the other groups when the comparison was independent from the grade. In terms of fixation points, there were differences between dentists and orthodontists and between orthodontists and laypeople. The fixation point mean was higher for orthodontists than for dentists and was similar to that for laypeople. CONCLUSIONS: Orthodontists were able to closely analyze the occlusal problems presenting with more severe malocclusions, and the specialists noticed more fixation points. Duration times, however, were not significantly different, because the orthodontists had greater interest, even in situations of unfavorable esthetics.

Pelvimetry of multiparous Nellore cows in the cycling and early puerperal stages / Pelvimetria de vacas pluríparas da raça Nellore em estágio cíclico e início do puerpério

Pelvis is an osteoligamentous complex, which is classified as dolichopellic in ruminants. Impairments linked to incompatibility of pelvic canal and fetal size is directly related to pelvic anatomy and disposition. Heritability of pelvic area characteristics varies from moderate to high, demonstrating its importance for animal selection. Pelvimetry can be performed through direct and indirect methods that were accessed in this study aiming to establish a correlation between internal and external pelvic dimensions in multiparous Nellore cows (26 animals) at cycling and early puerperal (up to 30 days postpartum) reproductive stages. Pelvic dimensions measured by descriptive analysis were higher at early puerperal stage. Strong Pearson's correlation was determined between internal and external pelvic dimensions, between reproductive stages, and between pelvic girdle area and internal pelvic area. Significant Tukey's test differences between animals in internal and external dimensions were also found. According to the results, the pelvic anatomy of Nellore cows varies according to their reproductive stage. We detach that and provide data regarding the use of internal pelvic area for animal selection studies on this breed is determined and should be measured using the proposed formula, since it can confirm statistical differences in the areas of pelvic components between animals in the cycling and early puerperal stages.(AU)

A pelve é considerada um complexo osteoligamentoso, classificada como dolicopélvica em ruminantes. Prejuízos ligados à incompatibilidade canal pélvico/tamanho fetal relacionam-se diretamente à anatomia e disposição pélvica. A característica de área pélvica apresenta herdabilidade variando de moderada a alta, indicando sua importância na seleção animal. A pelvimetria inclui formas direta e indireta. Neste estudo utilizaram-se vinte e seis vacas multíparas, da raça Nelore, agrupadas de acordo com o estágio reprodutivo, cíclico e em início de estágio puerperal (até 30 dias pós-parto). Através deste, objetivou-se evidenciar a relação das medidas pélvicas internas e externas nos diferentes períodos reprodutivos e coletar obter dados referentes a utilização da área interna do hexágono no estudo pelvimétrico dessa espécie em estágio cíclico ou puerperal. Para tanto, realizou-se, a pelvimetria direta e indireta. Os resultados obtidos, pela análise descritiva, foram maiores para animais em início de estágio puerperal. Por meio da correlação de Pearson podem-se observar fortes correlações entre mensurações pélvicas internas e externas, também entre os grupos propostos, e forte correlação entre elipse pélvica e área interna do hexágono. Pelo teste Tukey diferenças significativas tanto em medidas internas e externas puderam ser demonstradas. Conclui-se que as condições anatômicas da pelve variam de acordo com o estágio reprodutivo do animal, que aferição da área pélvica utilizando a formula proposta para área do hexágono interno é relevante pois foi possível comprovar a diferenças estatísticas de áreas nas fases cíclica e puerperal.(AU)