DLX6 and MSX1 from saliva samples as potential predictors of mandibular size: A cross-sectional study.

INTRODUCTION: Morphologic features of the mandible are influenced by the genes of each individual. Mandible size is important to orthodontists because the mandible is the mechanism by which the lower face influences facial esthetics and dental function. To date, no biological marker has been identified that indicates eventual mandible size. This study aimed to correlate the expression of DLX5, DLX6, EDN1, HAND2, PRRX1, and MSX1 to mandible size. METHODS: Fifty-nine orthodontic patients aged >6 years who had available cephalometric radiographs were studied. Patients were classified on the basis of condylion-to-gnathion measurements. Messenger RNA was isolated from saliva and subjected to real-time quantitative polymerase chain reaction. RESULTS: Threshold cycle values for subjects with small mandibles (>1 standard deviation [SD] from the mean) had the least expression of DLX6 and MSX1. Threshold cycle values for subjects with large mandibles (>1 SD) had less expression of DLX6 and MSX1 than subjects within 1 SD but more than those with small mandibles. CONCLUSIONS: DLX6 and MSX1 are related to mandible development and size. This finding could be used to improve treatment planning for medical and dental professionals seeking to understand the impact of genetics on bone growth.

The Class II Carriere Motion appliance.

OBJECTIVES: To determine three-dimensional treatment changes produced by the Class II Carriere Motion appliance (CMA) in Class II adolescent patients with Class I and Class II skeletal relationships. MATERIALS AND METHODS: The sample included 59 adolescents (16 boys and 43 girls) with unilateral or bilateral Class II molar and bilateral Class II canine relationship. They were divided into group 1 with skeletal Class I (N = 27; ANB 2.90° ± 1.40°; 13.30 ± 1.53 years) and group 2 with skeletal Class II (N = 32; ANB 6.06° ± 1.64°; 13.26 ± 1.76 years). Cone beam computed tomography images were traced with Invivo software pretreatment (T1) and post-CMA usage (T2). The treatment changes in 36 measurements were calculated in each group, and the changes in 16 measurements were compared between them. RESULTS: In group 1 and 2, maxillary first molars underwent significant distal movement (1.92 mm ± 0.80 mm and 1.67 mm ± 1.56 mm, respectively) with distal tipping and rotation, maxillary canines underwent significant distal movement (2.34 mm ± 1.07 mm and 2.24 mm ± 1.91 mm, respectively) with distal tipping and rotation, and mandibular molars underwent significant mesial movement (-1.37 mm ± 1.23 mm and -2.51 mm ± 1.51 mm, respectively) with mesial tipping. Between the groups, there were significant differences in mandibular molar mesial movement and the U1-SN changes (P < .05). CONCLUSIONS: The CMA corrected Class II malocclusion through distal tipping and rotational movement of maxillary canines and molars and corrected mesial tipping of mandibular molars. Significantly more mandibular molar mesial movement and maxillary incisor flaring were observed in patients with skeletal Class II.

Nasolabial Changes Following Double Jaw Surgery.

BACKGROUND: This study aimed to analyze 3-dimensional nasolabial soft tissue changes following double jaw surgery with Le Fort I maxillary advancement and relate them to underlying surgical movements. METHODS: Pre- and post-surgical cone-beam computed tomography images of 37 maxillomandibular surgery patients with ≥2 mm of advancement at A-point were analyzed. Lateral cephalograms were constructed to determine horizontal and vertical surgical movements and 3-dimensional facial images were created from the CBCTs to calculate linear and angular changes. RESULTS: Alar base width increased 1.9 mm, nasal tip protrusion decreased 0.74 mm, the upper lip flattened 7.71 degree and increased 0.9 mm in length, nasal tip was displaced 2.47 mm superiorly causing an increased concavity of 5.52 degree for the overall dorsal surface and 3.99 degree at the supratip break angle (SBA), nasal tip angle decreased 2.16 degree and nasolabial angle decreased 7.37 degree. Correlations were found between underlying surgical movements and nasal tip protrusion, SBA, nasolabial angle (NLA), and nasal tip elevation (NTE). Multiple linear regression equations were calculated to predict changes in SBA from horizontal change at A-point, NLA from horizontal change at B-point, and NTE from the change at A-point horizontally and U1-tip vertically. CONCLUSION: Double jaw surgery with maxillary advancement has significant effects on nasolabial anatomy.

Long-term stability of maxillary and mandibular arch dimensions when using rapid palatal expansion and edgewise mechanotherapy in growing patients.

OBJECTIVE: The purpose of this study was to assess the long-term stability of rapid palatal expansion (RPE) followed by full fixed edgewise appliances. METHODS: This study included 67 patients treated using Haas-type RPE and non-extraction edgewise appliance therapy at a single orthodontic practice. Serial dental casts were obtained at three different time points: pretreatment (T1), after expansion and fixed appliance therapy (T2), and at long-term recall (T3). The mean duration of the T1-T2 and T2-T3 periods was 4.8 ± 3.5 years and 11.0 ± 5.4 years, respectively. The dental casts were digitized, and the computed measurements were compared with untreated reference data. RESULTS: The majority of treatment-related increases in the maxillary and mandibular arch measurements were statistically significant (p < 0.05) and greater than expected for the untreated controls. Although many measurements decreased postretention (T2-T3), the net gains persisted for all of the measurements evaluated. CONCLUSIONS: The use of RPE therapy followed by full fixed edgewise appliances is an effective method for increasing maxillary and mandibular arch width dimensions in growing patients.

Accuracy of clear aligners: A retrospective study of patients who needed refinement.

INTRODUCTION: The purpose of this study was to determine the accuracy of specific tooth movements with Invisalign (Align Technology, Santa Clara, Calif). METHODS: The study sample included 20 Class I adult patients treated with Invisalign; they completed their first series of aligners and had to have a "refinement" series. Initial and predicted models were obtained from the initial ClinCheck (Align Technology). The starting point of the refinement ClinCheck was used to create the achieved models. Predicted and achieved models were superimposed over the initial ones on posterior teeth using the 3-dimensional Image Analysis open-source software Slicer CMF. Three hundred ninety-eight teeth were measured for vertical, horizontal, and rotational movements, and transverse widths were measured. The amount of predicted tooth movement was compared with the achieved amount for each movement. RESULTS: Horizontal movements of all incisors seemed to be accurate, with small (0.20-0.25 mm) or insignificant differences between predicted and achieved amounts. Vertical movements and particularly intrusions of maxillary central incisors were found to be less accurate, with a median difference of 1.5 mm (P <0.001). All achieved rotations were significantly smaller than those predicted, with the maxillary canines exhibiting the greatest difference of 3.05° (P <0.001). CONCLUSIONS: The most inaccurate movements identified in this study were intrusion of the incisors and rotation of the canines.

Orthodontic treatment of a patient with maxillary lateral incisors with dens invaginatus: 6-year follow-up.

INTRODUCTION: Dens invaginatus is an anomaly of dental development in which calcified tissues, such as enamel and dentin, are invaginated into the pulp cavity. This morphologic alteration is more frequent in maxillary permanent lateral incisors and makes them more susceptible to carious lesions and pulp alterations. METHODS: This case report describes a patient with maxillary lateral incisors affected by dens invaginatus. The maxillary right lateral incisor had already undergone endodontic treatment, and the maxillary left one had a periapical lesion. Additionally, the patient had a Class II Division 1 malocclusion, with anterior open bite, posterior crossbite, and an impacted mandibular left second molar. RESULTS: The orthodontic treatment involved extraction of the maxillary lateral incisors and 2 mandibular premolars, resulting in proper overjet and overbite with good arch coordination and occlusal stability. CONCLUSIONS: Treatment results were stable, as evaluated in a 6-year posttreatment follow-up.

Mandibular asymmetries and associated factors in orthodontic and orthognathic surgery patients.

OBJECTIVES: To estimate the prevalence of mandibular asymmetries in orthodontic and orthognathic surgery patients and to investigate demographic and skeletal factors associated with this disharmony. MATERIALS AND METHODS: Cone-beam computed tomography images of 1178 individuals aged 19 through 60 years with complete dentitions were analyzed. Outcomes were classified as relative mandibular symmetry, moderate asymmetry, and severe asymmetry. Factors recorded included sex, age, side of mandibular deviation, sagittal jaw relationship, vertical skeletal pattern, angle of the cranial base, and maxillary asymmetry. Ordinal logistic regression was used to estimate simple and adjusted odds ratios (OR) for the individuals with moderate and severe mandibular asymmetry, as well as 95% confidence intervals. RESULTS: Prevalence values of 55.2%, 27.2%, and 17.6% were observed for relative mandibular symmetry, moderate asymmetry, and severe asymmetry, respectively. An independent association with the side of mandibular deviation and the presence of maxillary asymmetry was observed, both for subjects with moderate mandibular asymmetry (left side: OR = 1.50; 95% CI: 1.01-2.24 / maxillary asymmetry: OR = 2.07; 95% CI: 1.11-3.76) and for those with severe asymmetry (left side: OR = 2.09; 95% CI: 1.27-3.44 / maxillary asymmetry: OR = 4.93; 95% CI: 2.64-9.20). CONCLUSIONS: Moderate and severe mandibular asymmetries were present in 44.8% of the sample, being associated with the side of mandibular deviation and with maxillary asymmetry.

Pharyngeal airway evaluation after isolated mandibular setback surgery using cone-beam computed tomography.

INTRODUCTION: In this study, we investigated volumetric and dimensional changes to the pharyngeal airway space after isolated mandibular setback surgery for patients with Class III skeletal dysplasia. METHODS: Records of 28 patients who had undergone combined orthodontic and mandibular setback surgery were obtained. The sample comprised 17 men and 11 women. Their mean age was 23.88 ± 6.57 years (range, 18-52 years). Cone-beam computed tomography scans were obtained at 3 time points: before surgery, average of 6 months after surgery, and average of 1 year after surgery. Oropharyngeal, hypopharyngeal, and total volumes were calculated. The lateral surface and anteroposterior dimensions at the minimal axial areas for oropharyngeal and hypopharyngeal volumes and mean mandibular setback were determined. RESULTS: The mean mandibular setback was 9.93 ± 5.26 mm. Repeated measures analysis of variance determined an overall significant decrease between the means for 6 months and up to 1 year after surgery for oropharyngeal and hypopharyngeal volumes, anteroposterior at oropharyngeal, lateral surface at oropharyngeal, and anteroposterior at hypopharyngeal. No strong correlation between mandibular setback surgery and pharyngeal airway volumes or dimensions was determined. CONCLUSIONS: After mandibular setback surgery, pharyngeal airway volume, and transverse and anteroposterior dimensions were decreased. Patients undergoing mandibular setback surgery should be evaluated for obstructive sleep apnea and the proposed treatment plan modified according to the risk for potential airway compromise.

Quantitative assessment of the effectiveness of phase 1 orthodontic treatment using the American Board of Orthodontics Discrepancy Index.

INTRODUCTION: This retrospective study included a sample of 300 randomly selected patients from the archived records of Saint Louis University's graduate orthodontic clinic, St. Louis, Mo, from 1990 to 2012. The objective of this study was to quantify the changes obtained in phase 1 of orthodontic treatment and determine how much improvement, if any, has occurred before the initiation of the second phase. METHODS: For the purpose of this study, prephase 1 and prephase 2 records of 300 subjects were gathered. All were measured using the American Board of Ortodontics Discrepancy Index (DI), and a score was given for each phase. The difference of the 2 scores indicated the quantitative change of the complexity of the treatment. Paired t tests were used to compare the scores. Additionally, the sample was categorized into 3 groups according to the Angle classifications, and the same statistics were used to identify significant changes between the 2 scores. Analysis of variance was applied to compare the 3 groups and determine which had the most change. Percentages of change were calculated for the significant scores. RESULTS: The total DI score overall and the scores of all 3 groups were significantly reduced from before to after phase 1. Overall, 42% improvement was observed. The Class I group showed 49.3% improvement, the Class II group 34.5% and the Class III group 58.5%. Most components of the DI improved significantly, but a few showed negative changes. CONCLUSIONS: Significant reductions of DI scores were observed in the total sample and in all Angle classification groups. This indicates that early treatment reduces the complexity of the malocclusions. Only 2 components of the DI showed statistically significant negative changes.

Relationships between soft tissues in a posed smile and vertical cephalometric skeletal measurements.

INTRODUCTION: The purposes of this study were to measure the 3-dimensional parameters of the posed smile and to see whether there are any correlations with vertical cephalometric skeletal measurements. METHODS: Pretreatment records from a sample of 110 white girls between the ages of 12 and 18 years were gathered. The measurements of SN-GoGn, anterior facial height, and lower and upper facial height percentages were obtained from tracing lateral cephalograms. Superimposing the repose and the posed smile facial scans allowed for measurements to be obtained showing the movements in the x, y, and z dimensions of the upper and lower lips, the commissures, and the Cupid's bows. Correlations and multiple linear regression analyses were run to check for associations and predictive relationships between the cephalometric skeletal measurements and soft tissue changes. RESULTS: We found significant moderate correlations and weak correlations. Significant multiple regression models were found for intercommissural width, smile index, and lower lip in the y and z dimensions. CONCLUSIONS: There were moderate correlations showing that as SN-GoGn and anterior facial height increased, the interlabial gap increased as the smile index decreased. Significant relationships were found between certain hard tissue cephalometric measurements and the width of the smile as well as the movements of the lower lip.

Long-term stability of rapid palatal expansion in the mixed dentition vs the permanent dentition.

INTRODUCTION: Palatal expansion has been a popular and proven technique for transverse discrepancies used in orthodontics for decades. The short-term effectiveness of the technique is understood, yet questions remain regarding the long-term stability, with much debate surrounding the optimum treatment timing to initiate expansion for the most beneficial and stable results, especially with regard to mixed dentition treatment. The purpose of this study was to determine the long-term stability of palatal expansion performed in the mixed dentition vs the permanent dentition. METHODS: Fifty-four subjects were grouped as follows: 24 in the mixed dentition and 30 in the permanent dentition at the start of treatment. All patients had been treated with the Haas type of palatal expansion appliance followed by nonextraction fixed edgewise mechanics. Digitized dental casts were evaluated at 3 times: before treatment (T1), after treatment (T2), and at the long-term retention (T3). Intermolar widths were computed by subtracting measurements between time points, and comparisons between the groups were made to determine expansion and stability differences. RESULTS: Significant intermolar width increases were observed from T1 to T2 with significant relapses from T2 to T3, and an overall net gain remained at T3 in each group. No significant differences were found for relapse between the mixed and permanent dentitions. CONCLUSIONS: There are no differences for the long-term intermolar width stability in patients treated with palatal expansion in the mixed dentition vs the permanent dentition.

Posttreatment evaluation of maxillary canine positions in 15-year-old subjects.

INTRODUCTION: Esthetic improvement is a primary reason that people seek orthodontic treatment. The maxillary canine is considered by many to have great importance for both function and esthetics. Limited information is available about the position of the maxillary canine in relation to skeletal landmarks and whether the position can influence esthetic perceptions. The purposes of this study were to evaluate the normal maxillary canine position in relation to skeletal landmarks, to determine posttreatment 3-dimensional maxillary canine position with cone-beam computed tomography images, and to see whether maxillary canine position influences esthetic perceptions. METHODS: The Bolton standard template was used as the control sample, and the maxillary canine position was determined by implementing a Cartesian coordinate system. The right and left maxillary canines of 96 treated patients (48 boys, 48 girls; 15 years old) were analyzed by digitization of the cone-beam computed tomography volumes. The subjects' posttreatment smile photographs were ranked and quantified by 9 orthodontic residents who completed a Q-sort. Correlations were determined between canine positions and esthetic outcomes. RESULTS: The only difference between the right and left canine positions was the anteroposterior position of the root apex. Statistically significant sex differences were found for the superoinferior position of the right and left canine cusp tips, the mediolateral right and left canine root apices, and the mediolateral left canine cusp tips. No correlation was determined between the maxillary canine position and the esthetic perception. CONCLUSIONS: The maxillary canine position in relation to skeletal landmarks was determined and does not appear to significantly impact the esthetic perception, according to this study.

Surgical treatment of adolescent internal condylar resorption (AICR) with articular disc repositioning and orthognathic surgery in the growing patient--a pilot study.

BACKGROUND: The aim of this study was to better understand how surgical repositioning and stabilization of anteriorly displaced articular discs using the Mitek mini-anchor technique affects condylar growth in growing patients with adolescent internal condylar resorption (AICR). METHODS: Twenty-two adolescent patients diagnosed with AICR and anterior temporomandibular disc displacement were compared to untreated control subjects without AICR matched for age, sex, and Angle classification. Pre-surgical (T1 and T2) and post-surgical (T3 and T4) mandibular tracings were superimposed on natural stable structures to evaluate the horizontal, vertical, and total changes in the position of condylion. RESULTS: The treated group showed an overall decrease in condylar height pre-surgically and statistically significant changes in condylar growth direction between the pre- and post-surgical observation periods. Pre-surgically, the treated group showed significantly more posterior condylar growth than the control group; they also showed inferior condylar growth, while the controls showed superior growth. Controls and patients in the treated group showed no significant differences in condylar growth post-surgically. CONCLUSIONS: Adolescent patients diagnosed with AICR and anterior disc displacement treated with mandibular ramus and maxillary osteotomies, along with Mitek anchors to reposition internally deranged discs, showed post-surgical normalization of condylar growth.

Extraction vs no treatment: Long-term facial profile changes.

INTRODUCTION: Long-term soft tissue response to extraction orthodontic treatment has been a subject of interest for years. The purposes of this study were to investigate long-term soft tissue profile changes in an extraction sample and to compare them with profile changes in an untreated sample. METHODS: A premolar extraction-treated sample (n = 47) and an untreated control sample (n = 57) were studied. Descriptive statistics were collected, and individual t tests were used for comparison and contrast of the treated and untreated samples. RESULTS: We found that the untreated soft tissue profile changed in the downward and forward direction. The treated soft tissue profile change was similar, but with more of a forward component than in the untreated sample. Most noteworthy was the finding that the soft tissue profiles of both the untreated and the treated samples were similar at the end point. CONCLUSIONS: The following conclusions were derived from the study. (1) There was no substantive difference in the soft tissue profiles of the samples, but there were some differences in the directional changes between them. (2) The changes for the untreated sample were the greatest for the lips and the chin, with the change occurring in the downward and forward direction. (3) The soft tissue profiles of the extraction sample also had the greatest measurable changes in the lips and the chin, but the changes had more of a forward component than they did in the untreated sample. (4) Extraction treatment does not adversely impact soft tissue profile changes over time.

Mandibular rotation during the transitional dentition.

OBJECTIVE: To determine whether dentoalveolar changes or vertical condylar growth is more closely related to the true forward rotation of the mandible during the transition between the late primary and early mixed dentition stages of development. MATERIALS AND METHODS: The sample included 50 subjects (25 males and 25 females) with Class I (N  =  25) and Class II (N  =  25) molar relationships. They were selected based on the availability of lateral cephalograms at two developmental stages: T1: last film with complete primary dentition (5.8 ± 0.4 years) and T2: first film with permanent incisors and permanent molars fully erupted (8.0 ± 0.2 years). Seventeen landmarks were identified and 22 measurements were calculated. The mandibles at T1 and T2 were superimposed using natural reference structures in order to measure true mandibular rotation. RESULTS: The mandible underwent -2.4° ± 2.6° of true rotation, 1.9° ± 2.4° of remodeling, and -0.6° ± 1.8° of apparent rotation. There were no significant sex or Class differences in true rotation, remodeling, and apparent rotation. There was a moderate correlation (r  =  0.76) between true rotation and remodeling and a moderately low correlation (r  =  0.40) between true rotation and apparent rotation. There was a weak correlation between true rotation and SNA (r  =  0.28). True rotation was most closely associated with the increases in U1/S-N (r  =  -0.34), increases in U1/PP (r  =  -0.36), and decreases in Id-Me (r  =  0.36). CONCLUSIONS: Independent of sex and Class, the true mandibular rotation that occurred between the late primary and early mixed dentition was mostly masked by angular remodeling, resulting in limited amounts of apparent rotation. True rotation was significantly related to anterior dentoalveolar changes but not to the vertical growth changes that occurred.

Maxillary growth and maturation during infancy and early childhood.

OBJECTIVE: To describe maxillary growth and maturation during infancy and early childhood. MATERIALS AND METHODS: Serial cephalograms (N=210) of 30 subjects (15 females and 15 males) from the Bolton-Brush Growth Study were analyzed. Each subject had a series of six consecutive cephalograms taken between birth and 5 years of age, as well as one adult cephalogram. Twelve maxillary measurements (eight linear and four angular) and seven landmarks were used to characterize maxillary growth. Maturation of the linear measures was described as a percentage of adult status. RESULTS: Maxillary and anterior cranial base size increased in both sexes between 0.4 and 5 years of age. The linear anteroposterior (AP) measures (S-SE, SE-N, ANS-PNS) increased almost as much as the vertical measures (S-PNS, SE-PNS, N-A, N-ANS) over the first 5 years. After 5 years of age there was significantly more vertical than AP growth. The size and shape changes that occurred were greatest between 0.4 and 1 years; yearly velocities decelerated regularly thereafter. Overall linear growth changes that occurred between 0.5 and 5 years of age (a span of 4.5 years) were generally greater than the changes in maxillary growth that occurred between 5 and 16 years (a span of 11 years). The linear measures showed a gradient of maturation, with the AP measures being more mature than the vertical measures. Male maxillae were less mature than female maxillae at every age. CONCLUSIONS: The maxilla undergoes its greatest postnatal growth change during infancy and early childhood, when relative AP growth and maturation are emphasized.

Facial divergence and mandibular crowding in treated subjects.

OBJECTIVE: To understand the relationships between facial divergence, vertical growth, and postretention mandibular crowding. MATERIALS AND METHODS: Seventy-five white extraction patients were evaluated immediately posttreatment (15.4 years) and again 16.6 years later. Hyperdivergent subjects, subjects with open bite or severe deep bites were not evaluated. Changes in incisor irregularity and tooth-size arch-length discrepancies (TSALD) were evaluated and correlated with measures of divergence and skeletal growth. RESULTS: Incisor irregularity increased 0.9 mm and TSALD increased 0.7 mm after treatment; 68% of the subjects had less than 3.5 mm incisor irregularity at postretention. Male patients showed significantly more growth than female patients did. Female patients, who were significantly more hyperdivergent than male patients, showed weak to moderately weak associations between posttreatment facial divergence and crowding. Female posttreatment changes in anterior face height, lower incisor eruption, and changes in arch depth were also related to crowding; male patients showed moderate relationships between posttreatment changes in arch width and crowding. CONCLUSIONS: Greater vertical growth, incisor eruption, and especially facial divergence are related to greater posttreatment mandibular crowding.

Stability changes of miniscrew implants over time.

PURPOSE: To quantify in vivo changes in miniscrew implant (MSI) stability over time using resonance frequency analysis, and to determine if pilot holes and placement sites affect changes in MSI stability. MATERIALS AND METHODS: Twenty-two self-tapping MSIs (1.6 mm wide and 9 mm long) were placed in the maxillae of 2 adult beagle dogs (20 months old). The Osstell Mentor was used to measure the implant stability quotient (ISQ) weekly for 8 weeks. A split-mouth design was used to evaluate the effects of 1.1-mm wide, 3-mm deep pilot holes. RESULTS: The MSIs that failed showed significantly (P < .05) greater decreases in ISQ values during the first 3 weeks than the MSIs that remained stable. All of the MSIs that failed (41%) had been placed in nonkeratinized tissue. MSIs that remained stable throughout the study also showed significant decreases in ISQ values during the first 3 weeks, followed by increases during the fourth and fifth weeks. Changes in ISQ values of MSIs inserted into bone with and without pilot holes were comparable (P > .05). CONCLUSION: Stability of unloaded MSIs decreased during the first 3 weeks and increased thereafter. Although the effects of pilot holes on stability could not be confirmed, placement of MSIs into nonkeratinized tissue negatively affected their stability and increased the likelihood of failures.