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1.
Carbohydr Polym ; 264: 117983, 2021 Jul 15.
Article in English | MEDLINE | ID: mdl-33910712

ABSTRACT

Cooking-induced conversion of starch, the major carbohydrate in pulses, is crucial for the digestibility of the seed. The gelatinization-melting transition of lentil, bean and chickpea starches was studied using Differential Scanning Calorimetry at different temperatures (T values ranged from 20 to 160 °C) and water contents (X from 0.2 to 3 kg kg-1 db). Gelatinization and melting endotherms were successfully modeled as two desummed Gaussian functions. This modeling enabled to generate the degree of starch conversion for any T and X conditions, a valuable indicator that could be used in predictive cooking models. As previously reported for melting, the temperature of gelatinization was found to depend on moisture in a way that can be modeled using the Flory-Huggins equation. The results suggest that starch undergoes melting transition irrespective of water content. The similar starch conversion diagram for the three pulses suggest that starches have similar thermal behavior.


Subject(s)
Cicer/chemistry , Fabaceae/chemistry , Gelatin/chemistry , Lens Plant/chemistry , Starch/chemistry , Animals , Calorimetry, Differential Scanning/methods , Cooking , Humans , Models, Theoretical , Nutritive Value , Seeds/chemistry , Temperature , Water/chemistry
2.
J Clin Virol ; 54(1): 76-8, 2012 May.
Article in English | MEDLINE | ID: mdl-22336086

ABSTRACT

In European countries, epidemiology of hepatitis E virus (HEV) infection is not well known. Although, seroprevalence of HEV Immunoglobulin G reached a few percent in European women, no acute hepatitis E during pregnancy has been described so far. Here, we report a case of an autochthonous HEV genotype 3 infection in a 41-years-old pregnant woman living in a non-endemic country. The acute hepatitis had a spontaneous good outcome for the mother and the child. In non-endemic areas where Hepatitis E infections are emerging, unexplained cytolysis, whatever its level, in a pregnant woman could be investigated for HEV, using biological molecular and serology tools.


Subject(s)
Hepatitis E virus/isolation & purification , Hepatitis E/diagnosis , Pregnancy Complications, Infectious/diagnosis , RNA, Viral/genetics , Adult , Female , France , Genotype , Hepatitis E virus/classification , Hepatitis E virus/genetics , Humans , Infant , Infant, Newborn , Pregnancy , Treatment Outcome
3.
Euro Surveill ; 15(39): 19676, 2010 Sep 30.
Article in English | MEDLINE | ID: mdl-20929659

ABSTRACT

In September 2010, two cases of autochthonous dengue fever were diagnosed in metropolitan France for the first time. The cases occurring in Nice, southeast France, where Aedes albopictus is established, are evidence of dengue virus circulation in this area. This local transmission of dengue calls for further enhanced surveillance, active case finding and vector control measures to reduce the spread of the virus and the risk of an epidemic.


Subject(s)
Antigens, Viral/blood , Dengue Virus/isolation & purification , Dengue/diagnosis , Adolescent , Dengue/transmission , Dengue Virus/genetics , Dengue Virus/immunology , Enzyme-Linked Immunosorbent Assay , France , Humans , Immunoglobulin G/blood , Immunoglobulin M/blood , Male , Middle Aged , Molecular Typing , Population Surveillance , Reverse Transcriptase Polymerase Chain Reaction , Urban Population
4.
Arch Pediatr ; 15(8): 1283-8, 2008 Aug.
Article in French | MEDLINE | ID: mdl-18586472

ABSTRACT

AIM: To determine the impact of rapid influenza test (RIT) on the prescription of additional tests, antibiotics and oseltamivir, and the influence of oseltamivir on clinical signs and parents' day work stoppage. METHODS: Prospective study in the pediatric emergency department of Nice University Hospital from 29th January 2007 to 3rd March 2007 including children from 1 month to 6 years old with fever greater or equal to 38.5 degrees C for less than 48 h. Virologic research on nasopharyngeal aspiration was: immunofluorescence, cell culture and RIT Quickvue. Clinical informations, additional tests and treatments were registered for each child. An antiviral treatment (oseltamivir) was proposed to children older than 1 year with positive RIT. Evolution at 7 days was evaluated by phone contact. RESULTS: One hundred and seventy-seven children were included (mean age 24 months, sex-ratio 1.88). The RIT was positive in 42.3% (n=75). Compared with cell culture, the sensibility, specificity, positive predictive value and negative predictive value of the RIT were, respectively, 95.6, 91.6, 88 and 97%. Clinical signs significantly correlated to influenza were: impairment, rhinitis and acute otitis media. In the RIT positive group, there were significantly less additional tests (13 versus 36) and particularly urinalysis (5 versus 19), and more spreading in the family (p=0.0002). There was not any significant difference concerning hospitalizations, antibiotic prescriptions, or parents' day work stoppage. CONCLUSION: During influenza epidemic, in a pediatric emergency department, RIT allows a reduction of additional tests in febrile young children, particularly urinalysis.


Subject(s)
Alphainfluenzavirus/isolation & purification , Betainfluenzavirus/isolation & purification , Influenza, Human/diagnosis , Influenza, Human/epidemiology , Age Factors , Antiviral Agents/therapeutic use , Child , Child, Preschool , Chromatography/methods , Data Interpretation, Statistical , Emergency Service, Hospital , Female , France/epidemiology , Humans , Infant , Infant, Newborn , Influenza, Human/drug therapy , Influenza, Human/virology , Male , Oseltamivir/therapeutic use , Prospective Studies , Sex Factors , Time Factors
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