Alleviating heat stress effects in poultry: updates on methods and mechanisms of actions.

Heat stress is a threat that can lead to significant financial losses in the production of poultry in the world's tropical and arid regions. The degree of heat stress (mild, moderate, severe) experienced by poultry depends mainly on thermal radiation, humidity, the animal's thermoregulatory ability, metabolic rate, age, intensity, and duration of the heat stress. Contemporary commercial broiler chickens have a rapid metabolism, which makes them produce higher heat and be prone to heat stress. The negative effect of heat stress on poultry birds' physiology, health, production, welfare, and behaviors are reviewed in detail in this work. The appropriate mitigation strategies for heat stress in poultry are equally explored in this review. Interestingly, each of these strategies finds its applicability at different stages of a poultry's lifecycle. For instance, gene mapping prior to breeding and genetic selection during breeding are promising tools for developing heat-resistant breeds. Thermal conditioning during embryonic development or early life enhances the ability of birds to tolerate heat during their adult life. Nutritional management such as dietary manipulations, nighttime feeding, and wet feeding often, applied with timely and effective correction of environmental conditions have been proven to ameliorate the effect of heat stress in chicks and adult birds. As long as the climatic crises persist, heat stress may continue to require considerable attention; thus, it is imperative to explore the current happenings and pay attention to the future trajectory of heat stress effects on poultry production.

Transcriptomics analysis unveils key potential genes associated with brain development and feeding behavior in the hypothalamus of L-citrulline-fed broiler chickens.

High ambient temperature is a major environmental stressor affecting poultry production, especially in the tropical and subtropical regions of the world. Nutritional interventions have been adopted to combat thermal stress in poultry, including the use of amino acids. L-citrulline is a nonessential amino acid that is involved in nitric oxide generation and thermoregulation, however, the molecular mechanisms behind L-citrulline's regulation of body temperature are still unascertained. This study investigated the global gene expression in the hypothalamus of chickens fed either basal diet or L-citrulline-supplemented diets under different housing temperatures. Ross 308 broilers were fed with basal diet (CON) or 1% L-citrulline diet (LCT) from day-old, and later subjected to 2 environmental temperatures in a 2 by 2 factorial arrangement as follows; basal diet-fed chickens housed at 24°C (CON-TN); L-citrulline diet-fed chickens housed at 24°C (LCT-TN); basal diet-fed chickens housed at 35°C (CON-HS), and L-citrulline diet-fed chickens housed at 35°C (LCT-HS) from 22 to 42 d of age. At 42-days old, hypothalamic tissues were collected for mRNA analyses and RNA sequencing. A total of 1,019 million raw reads were generated and about 82.59 to 82.96% were uniquely mapped to genes. The gene ontology (GO) term between the CON-TN and LCT-TN groups revealed significant enrichments of pathways such as central nervous system development, and Wnt signaling pathway. On the other hand, GO terms between the CON-HS and LCT-HS groups revealed enrichments in the regulation of corticosteroid release, regulation of feeding behavior, and regulation of inflammatory response. Several potential candidate genes were identified to be responsible for central nervous system development (EMX2, WFIKKN2, SLC6A4 Wnt10a, and PHOX2B), and regulation of feed intake (NPY, AgRP, GAL, POMC, and NMU) in chickens. Therefore, this study unveils that L-citrulline can influence transcripts associated with brain development, feeding behavior, energy metabolism, and thermoregulation in chickens raised under different ambient temperatures.

Growth performance and physiological responses of broilers fed Stylosanthes hamata leaf meal in a hot tropical environment.

BACKGROUND: Forage plants are considered an essential source of vitamins, protein and energy and could decrease the intake of the conventional diets by up to 10% if young quality forage plants are offered and about 7% of a broiler's daily protein needs and 3% of their daily calorie needs can be met by pasture. However, there is a paucity of data on the quantification of the herbage intake in broiler chickens. OBJECTIVES: This trial was designed to determine the performance and physiological response of broilers fed diets containing Stylosanthes hamata leaf meal (SHLM) at 0%, 1%, 5%, 10%, 15% and 20% inclusion (weight for weight) in a basal diet of poultry. METHODS: Two hundred and forty-day-old Arbor Acres broiler chicks were used for this trial. They were allocated at random to each of the 6 dietary treatment groups with 4 replicates of 10 chickens each. The study lasted for 35 days, during which data were collected weekly. RESULTS: Results indicated no difference (p > 0.05) in body weight gain, feed consumption and feed conversion ratio of broiler chickens across all ages. Although the plasma triiodothyronine (T3 ) concentration of the birds was influenced at the sixth week of age, there was no consistency in the trend. At the eighth week of age, chickens on 15% SHLM had significantly (p < 0.05) higher cortisol (3.22 ng/mL) concentrations compared to 10% (3.13 ng/mL) and 20% (3.16 ng/mL) SHLM treatment groups. Treatment groups, regardless of the age of the chickens, had similar (p > 0.05) mean plasma creatinine values. CONCLUSIONS: The study concluded that SHLM could be incorporated into broiler feed up to 20% without a deleterious impact on physiology and performance indices.

Global trends and research frontiers on heat stress in poultry from 2000 to 2021: A bibliometric analysis.

Background: Heat stress remains a major environmental factor affecting poultry production. With growing concerns surrounding climate change and its antecedent of global warming, research on heat stress in poultry has gradually gained increased attention. Therefore, this study aimed to examine the current status, identify the research frontiers, and highlight the research trends on heat stress in poultry research using bibliometric analysis. Methods: The literature search was performed on the Web of Science Core Collection database for documents published from 2000 to 2021. The documents retrieved were analyzed for their publication counts, countries, institutions, keywords, sources, funding, and citation records using the bibliometric app on R software. Network analysis for co-authorship, co-occurrence, citation, co-citation, and bibliographic coupling was visualized using the VOSviewer software. Results: A total of 468 publications were retrieved, and over the past two decades, there was a gradual increase in the annual number of publications (average growth rate: 4.56%). China had the highest contribution with respect to the number of publications, top contributing authors, collaborations, funding agencies, and institutions. Nanjing Agricultural University, China was the most prolific institution. Kazim Sahin from Firat University, Turkey contributed the highest number of publications and citations to heat stress in poultry research, and Poultry Science was the most productive and the most cited journal. The top 10 globally cited documents mainly focused on the effects of heat stress, alleviation of heat stress, and the association between heat stress and oxidative stress in poultry. All keywords were grouped into six clusters which included studies on "growth performance", "intestinal morphology", "heat stress", "immune response", "meat quality", and "oxidative stress" as current research hotspots. In addition, topics such as; "antioxidants", "microflora", "intestinal barrier", "rna-seq", "animal welfare", "gene expression", "probiotics", "feed restriction", and "inflammatory pathways" were identified for future research attention. Conclusion: This bibliometric study provides a detailed and comprehensive analysis of the global research trends on heat stress in poultry over the last two decades, and it is expected to serve as a useful reference for potential research that will help address the impacts of heat stress on poultry production globally.

Effects of arginine replacement with L-citrulline on the arginine/nitric oxide metabolism in chickens: An animal model without urea cycle.

BACKGROUND: This study examined the efficacy of L-citrulline supplementation on the arginine/nitric oxide metabolism, and intestinal functions of broilers during arginine deficiency. A total of 288 day-old Arbor Acre broilers were randomly assigned to either an arginine deficient basal diet (NC diet), NC diet + 0.50% L-arginine (PC diet), or NC diet + 0.50% L-citrulline (NCL diet). Production performance was recorded, and at 21 days old, chickens were euthanized for tissue collection. RESULTS: The dietary treatments did not affect the growth performance of broilers (P > 0.05), although NC diet increased the plasma alanine aminotransferase, urate, and several amino acids, except arginine (P < 0.05). In contrast, NCL diet elevated the arginine and ornithine concentration higher than NC diet, and it increased the plasma citrulline greater than the PC diet (P < 0.05). The nitric oxide concentration in the kidney and liver tissues, along with the plasma and liver eNOS activities were promoted by NCL diet higher than PC diet (P < 0.05). In the liver, the activities of arginase 1, ASS, and ASL, as well as, the gene expression of iNOS and OTC were induced by PC diet greater than NC diet (P < 0.05). In the kidney, the arginase 1, ASS and ASL enzymes were also increased by PC diet significantly higher than the NC and NCL diets. Comparatively, the kidney had higher abundance of nNOS, ASS, ARG2, and OTC genes than the liver tissue (P < 0.05). In addition, NCL diet upregulated (P < 0.05) the mRNA expression of intestinal nutrient transporters (EAAT3 and PEPT1), tight junction proteins (Claudin 1 and Occludin), and intestinal mucosal defense (MUC2 and pIgR). The intestinal morphology revealed that both PC and NCL diets improved (P < 0.05) the ileal VH/CD ratio and the jejunal VH and VH/CD ratio compared to the NC fed broilers. CONCLUSION: This study revealed that NCL diet supported arginine metabolism, nitric oxide synthesis, and promoted the intestinal function of broilers. Thus, L-citrulline may serve as a partial arginine replacement in broiler's diet without detrimental impacts on the performance, arginine metabolism and gut health of chickens.

Dietary L-citrulline modulates the growth performance, amino acid profile, and the growth hormone/insulin-like growth factor axis in broilers exposed to high temperature.

Heat stress adversely affects the growth performance, muscle development, and protein metabolism in poultry. l-Citrulline (L-Cit), is a non-essential amino acid that is known to stimulate muscle protein synthesis under stress conditions. This study investigated whether L-Cit could influence the growth performance, amino acid profile, and protein metabolism in broilers exposed to high ambient temperature. In a 2 × 2 factorial arrangement, Arbor acre broilers (288 chickens) were fed with basal diet (CON) or 1% L-Cit supplemented diet and later subjected to either thermoneutral (TNZ: 24°C, 24 h/d) or heat stress (HS: 35°C for 8 h/d) environment for 21 days. The results showed that L-Cit diet promoted the body weight and body weight gain of broilers higher than the CON diet, and it further alleviated HS suppression of body weight and feed intake at certain periods (p < 0.05). Plasma urea, uric acid, glucose, and total cholesterol were elevated during HS, whereas, the triglyceride content was decreased (p < 0.05). Serum amino acids including citrulline, alanine, aspartate, and taurine were decreased by HS. L-Cit supplementation restored the citrulline level and alleviated HS induction of 3-methylhistidine (p < 0.05). L-Cit supplementation increased the plasma growth hormone (GH) and insulin-like growth factor-1 (IGF-1) concentration, as well as the GH concentration in the breast muscle (p < 0.05). The mRNA expression showed that HS elicited tissue-specific responses by upregulating some growth factors in the breast muscle, but downregulated the GH receptor, GH binding protein, and IGF-1 expression in the hypothalamus. L-Cit supplementation upregulated the GHRH and IGFBP2 expression in the hypothalamus. L-Cit also upregulated the expression of IGF-1R and IGFBP2 in the breast muscle of HS broilers. The total mTOR protein level in the breast muscle of HS broilers was also increased by L-Cit diet (p < 0.05). Therefore, this study demonstrated that HS negatively affected the growth performance of broilers and dysregulated the expression of growth factors related to protein metabolism. Contrarily, L-Cit promoted the growth responses of broilers via its stimulation of circulating GH/IGF-1 concentration. To certain extents, L-Cit supplementation elicited protective effects on the growth performance of HS broilers by diminishing protein catabolism.

Effects of dietary L-citrulline supplementation on nitric oxide synthesis, immune responses and mitochondrial energetics of broilers during heat stress.

L-Citrulline is a non-protein amino acid that promotes arginine recycling and muscle protein synthesis. This study investigated whether L-citrulline can exert these functions in heat-stressed chickens. Arbor acre broilers were fed either basal diets (Control) or basal diets supplemented with 1% L-citrulline (L-Cit). At 28 d old, broilers were subjected to two environmental temperatures, 35 °C for 8 h/d (HS) or 24 °C for 24 h/d (TNZ) for 2 weeks. The experiment was designed as a 2 by 2 factorial arrangement. Results showed that HS increased the core body temperature (CBT) and rectal temperature of broilers compared to the TNZ condition. The CBT and mean CBT decreased by ∼0.5oC in TNZ + L-Cit broilers compared to the TNZ + Control group (P < 0.05). L-Cit supplementation at TNZ significantly (P < 0.05) lowered the plasma malondialdehyde content but this was increased during HS. Exposure to HS significantly (P < 0.05) elevated plasma malondialdehyde compared to TNZ condition. Plasma immunoglobulin A, G, and M were increased (P < 0.05) by TNZ + L-Cit compared to the TNZ + Control group. Plasma nitric oxide and endothelial nitric oxide synthase (NOS) activity was increased during HS, whereas the total and inducible NOS enzymes were decreased. In addition, L-Cit supplementation increased both the inducible and endothelial NOS isoforms (P < 0.05). The mRNA expression of peroxisome proliferator-activated receptor γ coactivator 1-α, avian uncoupling protein, cytochrome C oxidase subunit 3, and ATP synthase F1 subunit beta (ATP5ß) in the breast muscle were downregulated (P < 0.05) during HS. However, L-Cit supplementation upregulated the mitochondrial transcription factor A and during HS, L-Cit increased ATP5ß expression similar to TNZ housed broilers. Therefore, this study demonstrates that dietary L-Cit can lower the body temperature, decrease lipid peroxidation and promote the immune status of broilers under thermoneutrality. Also, L-Cit would act to promote muscle ATP generation during heat stress in broilers.

Functional roles of taurine, L-theanine, L-citrulline, and betaine during heat stress in poultry.

Heat stress (HS) is an important environmental stress factor affecting poultry production on a global scale. With the rise in ambient temperature and increasing effects of global warming, it becomes pertinent to understand the effects of HS on poultry production and the strategies that can be adopted to mitigate its detrimental impacts on the performance, health, welfare, immunity, and survival of birds. Amino acids (AAs) have been increasingly adopted as nutritional modifiers in animals to ameliorate the adverse effects of HS. They are essential for protein synthesis, growth, maintenance, reproduction, immunity, stress response, and whole-body homeostasis. However, HS tends to adversely affect the availability, transport, absorption, and utilization of these AAs. Studies have investigated the provision of these AAs to poultry during HS conditions, and variable findings have been reported. Taurine, L-theanine, and L-citrulline are non-essential amino acids that are increasingly gaining attention as nutritional supplements in HS animals. Similarly, betaine is an amino acid derivative that possesses favorable biological properties which contributes to its role as a functional additive during HS. Of particular note, taurine is negligible in plants, while betaine, L-theanine, and L-citrulline can be found in selected plants. These nutrients are barely found in feed ingredients, but their supply has been shown to elicit important physiological roles including anti-stress effects, anti-oxidative, anti-inflammatory, gut promoting, and immunomodulatory functions. The present review provides information on the use of these nutritionally and physiologically beneficial nutrients as functional additives to poultry diets during HS conditions. Presently, although several studies have reported on the positive effects of these additives in human and murine studies, however, there is limited information regarding their utilization during heat stress in poultry nutrition. Therefore, this review aims to expound on the functional properties of these nutrients, their potentials for HS alleviation, and to stimulate further researches on their biological roles in poultry nutrition.

Research Note: Effects of dietary L-arginine on the production performance and gene expression of reproductive hormones in laying hens fed low crude protein diets.

The present study was designed to investigate whether L-arginine (Arg) supplementation would influence the production performance and reproductive traits of laying hens fed low crude protein (LCP) diets. Hy-Line brown laying hens were randomly assigned to dietary treatments of control, LCP, and LCP supplemented with 0.05, 0.10, 0.15, or 0.20% Arg for 7 wk. The results showed no significant variations on the production performance, and relative organ indexes of hens. However, significant transcriptional and structural changes were evident along the HPG axis of hens. Arg supplementation linearly upregulated (P < 0.05) the gonadotropin-releasing hormone 1 (GnRH1), and gonadotropin inhibitory hormone in the hypothalamus. The pituitary growth hormone, GnRH receptor, and follicle-stimulating hormone (FSHß) were also increased (P < 0.05). In the ovary, GnRH1, and estrogen receptor ß were linearly increased by Arg, and the ovarian morphology revealed that LCP induced structural alterations which were minimally recovered by Arg supplementation. Serum insulin-like growth factor 1 (IGF-1) and nitric oxide (NO) were increased (P < 0.05) at higher levels of Arg supplementation. Therefore, supplementing high Arg (0.20%) to LCP hens influenced the ovarian morphology and modulated the gene expression of reproductive hormones in the hypothalamic-pituitary-gonadal axis of laying hens via actions that may be related to NO and IGF-1 activity.

Dietary L-citrulline influences body temperature and inflammatory responses during nitric oxide synthase inhibition and endotoxin challenge in chickens.

Recent studies have revealed the role of L-citrulline (L-CIT) in thermoregulation, but very little is known about the mechanisms involved. In this study, nitric oxide synthase inhibition and endotoxin-induced fever were used to investigate the effects of L-CIT on body temperature and inflammatory responses. In experiment 1, NW-nitro-L-arginine methyl ester (L-NAME, 150 mg/kg BW), was i. p. injected into chicks fed with basal (CON) or L-CIT diets for 14 days. In experiment 2, Lipopolysaccharide (LPS, 2 mg/kg BW) was i. p. injected following 21d feeding with CON or L-CIT diets. In experiment 3, chickens were injected with either L-NAME, LPS, or L-NAME + LPS following 26 days feeding with CON or L-CIT diets. The rectal (RT), ear (ET), and core body temperature (CBT) of chickens were examined. Results showed that L-NAME effectively decreased the RT, ET, CBT, and plasma NO concentration. In contrast, LPS increased NO levels and initiated hyperthermia by increasing RT, ET, CBT, and PGE2 levels. L-CIT diet reduced the mean CBT in experiment 1 and diminished the NO level, PGE2 level, and mean RT in experiment 3. Co-administration of L-CIT + LPS upregulated IL-6 expression, whereas, LPS injection alone induced IL-10, IL-1ß, and TLR4 gene expressions. Therefore, this study reveals that L-CIT-induced hypothermia was related to NO inhibition and a decrease in PGE2 concentration. Conversely, LPS induced hyperthermia was associated with an increase in both NO and PGE2 concentrations.

L-Citrulline Influences the Body Temperature, Heat Shock Response and Nitric Oxide Regeneration of Broilers Under Thermoneutral and Heat Stress Condition.

Heat stress (HS) adversely affects several physiological responses in organisms, but the underlying molecular mechanisms involved are yet to be fully understood. L-Citrulline (L-Cit) is a nutraceutical amino acid that is gaining research interest for its role in body temperature regulation and nitric oxide synthesis. This study investigated whether dietary supplementation with L-Cit (1% of basal diet) could ameliorate the effects of acute HS on thermotolerance, redox balance, and inflammatory responses of broilers. Ross 308 broilers (288 chicks) were subjected to two environments; thermoneutral at 24°C (TNZ) or HS at 35°C for 5 h, and fed two diets; control or L-Cit. The results showed that HS increased the ear, rectal (RT), and core body (CBT) temperatures of broilers, along with higher respiratory rate. The RT and CBT readings were intermittently affected with time effect, whereas, L-Cit supplementation lowered the mean CBT than the control diet. Antioxidant assays showed that superoxide dismutase was increased during HS, while, catalase was promoted by L-Cit supplementation. In addition, L-Cit induced glutathione peroxidase activity compared to the control diet during HS. Hypothalamic heat shock protein (HSP)-90 was upregulated by HS, but L-Cit downregulated heat shock factor (HSF)-1, and HSP 60 mRNA expressions. HSF 3 mRNA expression was downregulated by L-Cit under TNZ condition. More so, HS increased the plasma nitric oxide (NO) concentration but lowered the total NO synthase (tNOS) activity. In contrast, L-Cit supplementation limited NO production but increased the tNOS activity. Arginase activity was increased in the control fed group during HS but L-Cit supplementation lowered this effect. The NOS-COX pathway was significantly affected under TNZ condition, since L-Cit supplementation downregulated the mRNA expression of iNOS-COX2 in the hypothalamus, and further reduced the serum PGE2 concentration. Together, these data indicates that L-Cit influenced the antioxidant defense, heat shock response and nitric oxide regeneration both under thermoneutral and HS conditions; and that L-Cit may be directly and/or indirectly involved in the central regulation of body temperature.

Evaluation of access to different legume pastures on performance and welfare of broiler chickens during dry season under tropical environment.

There have been a plethora of studies on the effects of access to runs on chickens' welfare and behavioural repertoire with a paucity of information on the comparative advantage of various legume pasture and deep litter system. A total of 200-day-old unsexed Marshall Broiler chicks were weighed and assigned randomly into five experimental groups, viz. deep litter without access to run (DL), deep litter with access to Stylosanthes hamata (SH), Stylosanthes guanensis (SG), Mucuna pruriens (MP) and free run (FR) during dry season. Each treatment had 4 replicates of 10 birds. Data were collected on growth performance, behaviour, tonic immobility (TI), gait score and blood parameters. The data obtained were subjected to One-Way Analysis of Variance in a Completely Randomized Design. Results showed that the final body weight of birds in SH was significantly higher (p Ë‚ .05) than those of SH, MP, FR and DL which were comparable. The feed intake of the birds of DL, SH and FR was higher than those of the other treatment groups. The feed conversion ratio (FCR) of the birds on legume pastures was lower than those without access to pasture. The birds of SH, SG and MP spent higher (p Ë‚ .05) time drinking, preening, dust-bathing, spot pecking and walking standing behaviours while those in DL and FR spent more (p Ë‚ .05) time feeding. Generally, the gait score of the birds on the different legume pastures was similar but better than those without access to pasture. Tonic immobility of the DL birds was longer than that of FR whose duration was longer than those of the birds on the pastures. The study concluded that access to different legume pastures, particularly Stylosanthes hamata, improved the welfare of broiler chickens without adverse effect on the performance of the birds.

Blastodermal development, hatchability and chick quality of Marshall® broiler breeders of different flock ages during egg storage.

The blastoderm, which represents the fertilized germinal disc, undergoes cellular events of division, differentiation and organization to achieve embryonic development in chickens. In this study, blastodermal cell counts and hatching performance of Marshall® broiler breeders as influenced by flock age and egg storage were investigated. A total of 1,520 eggs from the flock ages, 43 weeks (younger) and 65 weeks (older), under similar management and nutritional regime were incubated after storage at 16°C with 75% relative humidity for duration of 0 (fresh eggs), 4, 7 and 10 days. Results show that total blastodermal cell counts (BCC) for both ages declined with increasing storage duration, with a sharp regression at 4d storage from younger breeders. Higher percentages of fertility and hatchability were recorded for younger breeders compared to older breeders. Percentage hatchability was statistically similar (p > .05) for both 43 weeks and 65 weeks old broiler breeders under 0, 4 and 7d storage except at 10d storage which was lower for older breeders. Chick lengths were longer with younger breeders whereas older breeders had heavier chick weights. Also, significant associations were found between BCC and fertility, hatchability of fertile eggs and chick weights, respectively, under different conditions. These results indicate that fresh eggs (0d) and short-term stored eggs (4- and 7 d) from 43 weeks old breeders had higher total blastodermal cell counts, fertility, hatchability and increased chick lengths compared to 65 week old breeders which produced heavier chicks. Therefore, egg storage longer than 7 days especially from older breeders resulted in modifications to the blastoderm which subsequently affected fertility, hatchability, embryo liveability and hatched chick quality. Also, detection of BCC in fertile eggs can serve as a predictive tool in fertility and hatchability evaluations.

Use of Moringa oleifera leaves in broiler production chain. 1 - Effect on Sasso breeder hens performances, internal quality of hatching eggs and serum lipids.

An experiment was conducted to determine the effect of Moringa oleifera leaves (MOL) on breeder hen performance, hatching egg quality and blood parameters. A total of 180 Sasso broiler breeder hens were used for the study. They were allotted to two groups each containing 3 replicates of 30 hens of 18 weeks of age. Dietary treatments were a control diet (basal diet without moringa leaves) and a diet containing 10g of moringa leaves in 1kg of control diet (1% w/w). The experiment lasted 40 weeks. The following production parameters; feed intake, egg weight, laying rate and feed conversion ratio were measured for the whole experimental period while egg quality and blood parameters were assessed at different ages. Results showed that laying rate and hatching egg weight were improved by moringa meal diet for the whole experimental period. Feed intake and feed conversation ratio were decreased (p < .05) by incorporation of moringa leaves. Differences (p < .01) were observed in albumen and yolk ratio at 55th week of age. Yolk colour was (p < .001) improved by moringa meal at 31st, 43rd and 55th week of age. At the end of the experiment, serum concentrations of triglycerides, total cholesterol, low-density lipoprotein cholesterol and atherogenic index were decreased (p < .05) while high-density lipoprotein cholesterol was unaffected (p > .05) by incorporation of moringa in the diet. It was concluded that the use of moringa leaves as feed additive in Sasso breeders diet improved productivity and quality of hatching eggs.

Prevalence of mange among West African Dwarf sheep and goats and associated haematological and biochemical parameters.

This study was carried out to assess the prevalence of mange in sheep and goats in five local government areas (LGAs) of Ogun State in South Western Nigeria. A total of 4,973 sheep and 7,902 goats of West African Dwarf breeds were sampled of which only 4 (0.08%) of sheep and 42 (0.53%) of goats were confirmed positive for mange infestation. In all the LGAs sampled, Odeda LGA had the highest concentration of sheep (39%) and goats (51%) while Abeokuta South had the least percentage of sheep (1%) and goats (1%). All the mange-infested cases in sheep were recorded in just one LGA (Ewekoro) which constituted 0.24% of the population within the location. For goats, three out of five LGAs namely: Obafemi Owode, Ewekoro and Abeokuta North had prevalence of 1.46%, 0.53% and 0.72% respectively. There was no significant (P > 0.05) difference between non-infested and infested animals in terms of packed cell volume (31.79 ± 0.87% vs. 31.41 ± 1.13%), haemoglobin (10.51 ± 0.21 g/100 ml vs. 10.28 ± 0.37 g/100 ml) and red blood cells (8.71 ± 0.29 × 10(12)/l vs. 9.40 ± 0.37 × 10(12)/l). The infested group however showed significantly (P < 0.01) higher white blood cells count (7.60 ± 0.22 × 10(9)/l) than the non-infested animals (6.81 ± 0.17 × 10(9)/l). Neutrophil, eosinophil, basophil and monocyte as well as chloride were not significantly affected by the health status of the animals. There existed significant (P < 0.01) difference between non-infested and infested animals in terms of total protein (5.42 ± 0.16 vs. 4.75 ± 0.20 g/dl), sodium (137.98 ± 1.53 vs. 128.92 ± 1.92 mEq/l) and potassium (4.04 ± 0.14 vs. 3.46 ± 0.18 mEq/l). It can be concluded from this study that the prevalence of mange mite infestation varied with location and was generally low or absent in some of the LGAs considered. Goats were particularly more susceptible to mange infestation than sheep. More concerted effort is needed to control mange in order to avoid spread since it is a contagious disease.