ABSTRACT
It may be possible to achieve insulin sensitivity through the recently identified mitochondrial target of thiazolidinediones (mTOT), thereby avoiding peroxisome proliferator-activated receptor-γ (PPAR-γ)-dependent side effects. In this phase IIb clinical trial, 258 patients with type 2 diabetes completed a 12-week protocol with 50, 100, or 150 mg of MSDC-0160 (an mTOT modulator), 45 mg pioglitazone HCl (a PPAR-γ agonist), or a placebo. The two active treatments lowered fasting glucose levels to the same extent. The decreases in glycated hemoglobin (HbA1c) observed with the two higher doses of MSDC-0160 were not different from those associated with pioglitazone. By contrast, fluid retention as evidenced by reduction in hematocrit, red blood cells, and total hemoglobin was 50% less in the MSDC-0160-treated groups. There was also a smaller increase in high-molecular-weight (HMW) adiponectin with MSDC-0160 than with pioglitazone (P < 0.0001), suggesting that MSDC-0160 produces less expansion of white adipose tissue. Thus, mTOT modulators may have glucose-lowering effects similar to those of pioglitazone but without the adverse effects associated with PPAR-γ agonists.
Subject(s)
Diabetes Mellitus, Type 2/drug therapy , Hypoglycemic Agents/therapeutic use , Insulin Resistance , Mitochondrial Proteins/drug effects , Pyridines/therapeutic use , Thiazolidinediones/therapeutic use , Adiponectin/metabolism , Blood Glucose/drug effects , Diabetes Mellitus, Type 2/blood , Diabetes Mellitus, Type 2/complications , Dose-Response Relationship, Drug , Edema/blood , Edema/complications , Female , Glycated Hemoglobin/metabolism , Humans , Hypoglycemic Agents/adverse effects , Male , Middle Aged , Pioglitazone , Pyridines/adverse effects , Thiazolidinediones/adverse effectsABSTRACT
Apparently favorable effects of antiatherosclerosis drugs as assessed by changes in surrogate markers of cardiovascular disease risk are frequently relied upon for drug approval and labeling. Surrogates must be biologically plausible and adequately validated but are, by definition, imperfect as predictors of ultimate outcome (i.e., serious morbidity and mortality). Surrogate markers utilized in the study of drugs for the treatment of atherosclerotic cardiovascular disease may be classified as laboratory/biochemical, anatomic/morphologic, and functional. The places for various surrogates in all three categories in the development of lipid altering drugs are discussed.
Subject(s)
Coronary Artery Disease/blood , Drug Evaluation , Hypolipidemic Agents/therapeutic use , Lipids/blood , Biomarkers/blood , Clinical Trials as Topic/methods , Coronary Artery Disease/drug therapy , Drug Approval , Drug Evaluation/methods , Humans , Reproducibility of ResultsSubject(s)
Coronary Disease/prevention & control , Hydroxymethylglutaryl-CoA Reductase Inhibitors/therapeutic use , Hypercholesterolemia/drug therapy , Age Factors , Clinical Trials as Topic , Coronary Disease/complications , Female , Humans , Hypercholesterolemia/complications , Male , Recurrence , Risk Factors , Sex FactorsSubject(s)
Arteriosclerosis/etiology , Diabetes Mellitus, Type 2/complications , Lipids/blood , Arteriosclerosis/blood , Arteriosclerosis/drug therapy , Blood Glucose/metabolism , Diabetes Mellitus, Type 2/blood , Diabetes Mellitus, Type 2/drug therapy , Drug Therapy, Combination , Humans , Hyperlipidemias/blood , Hyperlipidemias/complications , Hyperlipidemias/drug therapy , Hypoglycemic Agents/therapeutic use , Hypolipidemic Agents/therapeutic use , Risk FactorsSubject(s)
Arteriosclerosis/drug therapy , Clinical Trials as Topic/standards , Drug Evaluation/standards , Drug and Narcotic Control/legislation & jurisprudence , Legislation, Drug/standards , Arteriosclerosis/blood , Drug Design , Humans , Hypolipidemic Agents/therapeutic use , Safety , United States , United States Food and Drug AdministrationSubject(s)
Clinical Trials as Topic/methods , Drug Evaluation/methods , Hyperlipidemias/drug therapy , Hypolipidemic Agents/therapeutic use , Lipids/blood , Animals , Arteriosclerosis/blood , Arteriosclerosis/diagnostic imaging , Arteriosclerosis/prevention & control , Female , Guinea Pigs , Humans , Hyperlipidemias/blood , Male , Radiography , Safety , Treatment OutcomeABSTRACT
Tumor glucose use in patients with non-islet-cell tumors has been difficult to measure, particularly in hepatoma, because of hepatic involvement by neoplasm. We studied a patient with nonhepatic recurrence of hepatoma after successful liver transplantation. Tumor tissue contained messenger RNA for insulin-like growth factor-II (IGF-II), and circulating high molecular weight components and E-peptide of IGF-II were increased. Glucose use measured by isotope dilution with [3-3H]glucose was 7.94 mg/kg fat-free mass per min, and splanchnic glucose production was 0.93 mg/kg fat-free mass per min. Glucose uptake and glucose model parameters were independently measured in tissues by positron emission tomography with 18F-fluoro-2-deoxy-D-glucose. Glucose uptake by heart muscle, liver, skeletal muscle, and neoplasm accounted for 0.8, 14, 44, and 15% of total glucose use, respectively. Model parameters in liver and neoplasm were not significantly different, and glucose transport and phosphorylation were twofold and fourfold greater than in muscle. This suggests that circulating IGF-II-like proteins are partial insulin agonists, and that hypoglycemia in hepatoma with IGF-II production is predominantly due to glucose uptake by skeletal muscle and suppression of glucose production.
Subject(s)
Carcinoma, Hepatocellular/metabolism , Glucose/metabolism , Hypoglycemia/metabolism , Liver Neoplasms/metabolism , Adult , Analysis of Variance , Carcinoma, Hepatocellular/complications , Humans , Hypoglycemia/complications , Kinetics , Liver Neoplasms/complications , Liver Transplantation , Male , Tomography, Emission-ComputedABSTRACT
Post-transcriptional regulation of genes important in iron metabolism, ferritin and the transferrin receptor (TfR), is achieved through regulated binding of a cytosolic protein, the iron-responsive element binding protein (IRE-BP), to RNA stem-loop motifs known as iron-responsive elements (IREs). Binding of the IRE-BP represses ferritin translation and represses degradation of the TfR mRNA. The IRE-BP senses iron levels and accordingly modifies binding to IREs through a novel sensing mechanism. An iron-sulfur cluster of the IRE-BP reversibly binds iron; when cytosolic iron levels are depleted, the cluster becomes depleted of iron and the IRE-BP acquires the capacity to bind IREs. When cytosolic iron levels are replete, the IRE-BP loses RNA binding capacity, but acquires enzymatic activity as a functional aconitase. RNA binding and aconitase activity are mutually exclusive activities of the IRE-BP, and the state of the iron-sulfur cluster determines how the IRE-BP will function.
Subject(s)
Ferritins/genetics , Gene Expression Regulation , RNA, Messenger/metabolism , RNA-Binding Proteins/metabolism , Receptors, Transferrin/genetics , Aconitate Hydratase/metabolism , Amino Acid Sequence , Animals , Humans , Iron/metabolism , Iron-Regulatory Proteins , Molecular Sequence Data , RNA-Binding Proteins/chemistry , Receptors, Transferrin/metabolism , Sulfur/metabolismABSTRACT
A clone for the iron-responsive element (IRE)-binding protein (IRE-BP) has been transfected and expressed in mouse fibroblasts. The IRE-BP gene product binds IREs with high affinity and specificity. Amino acid alignments reveal that the IRE-BP is 30% identical to mitochondrial aconitase. The 18 active site residues of mitochondrial aconitase are identical to those in the IRE-BP, suggesting that the IRE-BP may possess aconitase activity. After purification of native IRE-BP and immunoaffinity purification of transfected and expressed IRE-BP, we demonstrate that the purified IRE-BP has aconitase activity.
Subject(s)
Aconitate Hydratase/metabolism , RNA-Binding Proteins/metabolism , Aconitate Hydratase/genetics , Amino Acid Sequence , Animals , Cells, Cultured , Chimera , Cloning, Molecular , DNA/genetics , Ferritins/metabolism , Genes, myc , Humans , Iron-Regulatory Proteins , Mice , Molecular Sequence Data , Open Reading Frames , RNA-Binding Proteins/genetics , Restriction Mapping , Transcription, Genetic , TransfectionABSTRACT
Stimulation of T cells by antigen activates many signalling pathways. The capacity for this range of biochemical responses may reside in the complex structure of the seven-chain T-cell antigen receptor (TCR). In addition to the complexity shared by all TCRs, coexpression of zeta (zeta) and the distinct but related eta (eta) chains creates structural diversity among the TCR complexes expressed on a given cell. In most murine T cells that we have studied, about 90% of the heptameric receptor complexes contain a zeta zeta disulphide homodimer, whereas 10% contain a zeta eta disulphide heterodimer. Recent studies suggest that zeta has a critical role in allowing antigen to activate the cell, whereas eta expression has been correlated with the capacity for antigen-induced phosphoinositide turnover. A third zeta-related protein, the gamma (gamma) chain of the Fc epsilon and some Fc gamma receptors, exists as a disulphide homodimer in those complexes. The structural relatedness of zeta and gamma is emphasized by the recent demonstration of zeta zeta in association with CD16 in TCR-negative natural killer cells. Here we identify T cells lacking Fc receptors but coexpressing zeta, gamma, and eta, document the formation of novel heterodimers between zeta and gamma and between eta and gamma and show their association with the TCR. A greater range of homologous coupling structures than previously thought may be one way of achieving the variety of TCR-mediated (and possibly Fc receptor-mediated) biochemical responses and effector functions.
Subject(s)
Disulfides , Receptors, Antigen, T-Cell/analysis , Receptors, Fc/analysis , T-Lymphocytes/immunology , Animals , Cell Line , DNA Probes , Electrophoresis, Gel, Two-Dimensional , Humans , Hybridomas/analysis , Immunoblotting , Immunosorbent Techniques , Macromolecular Substances , Mast Cells/analysis , Mice , Molecular Weight , Nucleic Acid Hybridization , RNA, Messenger/analysis , Receptors, Antigen, T-Cell/genetics , Receptors, Fc/genetics , T-Lymphocytes, Cytotoxic/analysisABSTRACT
T cell hybridomas that express zeta zeta, but not zeta eta, dimers in their T cell receptors (TCRs) produce interleukin-2 (IL-2) and undergo an inhibition of spontaneous growth when activated by antigen, antibodies to the receptor, or antibodies to Thy-1. Hybridomas without zeta and eta were reconstituted with mutated zeta chains. Cytoplasmic truncations of up to 40% of the zeta molecule reconstituted normal surface assembly of TCRs, but antigen-induced IL-2 secretion and growth inhibition were lost. In contrast, cross-linking antibodies to the TCR activated these cells. A point mutation conferred the same signaling phenotype as did the truncations and caused defective antigen-induced tyrosine kinase activation. Thus zeta allows the binding of antigen/major histocompatibility complex (MHC) to alpha beta to effect TCR signaling.
Subject(s)
Interleukin-2/biosynthesis , Lymphocyte Activation , Peptide Fragments/immunology , Receptors, Antigen, T-Cell/immunology , T-Lymphocytes/immunology , Amino Acid Sequence , Animals , Cross-Linking Reagents , Dose-Response Relationship, Immunologic , Hybridomas , Immunity, Cellular , Immunoblotting , Ligands , Major Histocompatibility Complex , Mice , Molecular Sequence Data , Mutation , Peptide Fragments/genetics , Precipitin Tests , Receptors, Antigen, T-Cell/genetics , Signal Transduction , TransfectionABSTRACT
The zeta (zeta) chain plays a central role in T cell antigen receptor assembly and signal transduction. From previous work in murine T cell hybridomas we have inferred that the zeta subunit is limiting in receptor assembly. Partial receptors made in excess of zeta are assembled in the endoplasmic reticulum, transported through the Golgi, but then rapidly and efficiently degraded in lysosomes. zeta would therefore seem to play a unique role in targeting receptors from the Golgi to the cell surface. To determine directly whether zeta limits receptor assembly we have reconstituted a zeta-deficient T cell line by transfection of the murine zeta cDNA. Transfection results in restoration of expression of surface T cell receptor. In addition, increasing zeta expression results in a commensurate increase in the survival of previously excess subunits. This is reflected in an increased surface expression of complete receptors. Finally, transfection of the zeta cDNA fails to produce detectable zeta-eta heterodimers. The implications of these findings with regard to receptor assembly, and the relationship between zeta and eta, are discussed.
Subject(s)
Membrane Glycoproteins/genetics , Receptors, Antigen, T-Cell/genetics , T-Lymphocytes/metabolism , Animals , Cell Line , DNA/metabolism , Gene Expression Regulation , Golgi Apparatus/metabolism , Membrane Glycoproteins/biosynthesis , Membrane Glycoproteins/physiology , Membrane Proteins/biosynthesis , Membrane Proteins/genetics , Membrane Proteins/isolation & purification , Mice , Protein Conformation , Receptors, Antigen, T-Cell/biosynthesis , Receptors, Antigen, T-Cell/physiology , Structure-Activity Relationship , T-Lymphocytes/physiology , TransfectionABSTRACT
The T-cell antigen receptor is a multisubunit complex consisting of at least seven chains. Based upon structural and genetic considerations, we have divided these chains into three groups. The alpha and beta subunits (Ti) are the clonotypic chains responsible for antigen recognition. Three chains that are invariant among all T-cells define the CD3 complex. These include the CD3 gamma, delta, and epsilon chains. The zeta chain is a distinct component that, like the CD3 chains, is invariant among all T-cells. In the majority of receptors, zeta is found as a disulfide-linked homodimer. We have recently shown that approximately 10% of zeta is disulfide-linked to a chain which we have called eta. A preliminary model has been proposed, suggesting that there are two subclasses of receptors, depending upon the presence within the complex of either the zeta-zeta homodimer or the zeta-eta heterodimer. Evidence has been presented that these two subclasses may perform distinct signaling functions. In this paper the eta chain is characterized to determine whether it is structurally related to the zeta chain and, in particular, whether it might represent a post-translational modification of zeta. We can identify specific antigenic epitopes that are shared by both zeta and eta. However, not all antibodies raised against zeta can directly recognize eta. The apparent molecular mass of eta is 22 kDa, whereas zeta has a molecular mass of 16 kDa. We are unable to demonstrate any post-translational covalent modifications of eta to explain the difference in apparent molecular weight. These include phosphorylation, glycosylation, or sulfation. Amino acid incorporation studies demonstrate that the amino acid composition of eta is distinct from that of zeta. All of the eta in a T-cell is found in association with the rest of the components of the T-cell receptor. In addition, our anti-eta antibodies allow us to directly recognize human eta, which has an apparent molecular mass of approximately 23 kDa. Thus, eta and zeta appear to be related but distinct proteins, and we would propose that eta is the second member of the zeta group of components of the T-cell receptor.
Subject(s)
Membrane Glycoproteins , Membrane Proteins , Receptors, Antigen, T-Cell , Amino Acid Sequence , Amino Acids/isolation & purification , Animals , Cross Reactions , Humans , Membrane Glycoproteins/genetics , Membrane Glycoproteins/immunology , Membrane Glycoproteins/metabolism , Mice , Molecular Sequence Data , Protein Processing, Post-Translational , Rabbits , Rats , Receptors, Antigen, T-Cell/genetics , Receptors, Antigen, T-Cell/immunology , Receptors, Antigen, T-Cell/metabolism , Structure-Activity Relationship , Transcription, GeneticABSTRACT
The T-cell antigen receptor (TCR) is a multisubunit receptor complex specific to T cells subserving both antigen recognition and signal transduction functions. The zeta chain of the TCR is a component of all surface receptor complexes. This chain was first identified in murine T cells by virtue of the fact that it coimmunoprecipitates with the TCR complex using antibodies directed against either the clone-specific subunits or invariant CD3 subunits of the receptor. Recently, we have isolated a cDNA encoding the murine zeta. Using this as a probe, we have now isolated cDNAs encoding the human zeta. Sequence analysis of cDNAs encoding human and murine zeta reveals that it is a highly conserved protein. In addition to amino acid homology, there is remarkable interspecies conservation in the nucleotide sequence of the 5' and 3' untranslated regions of the zeta mRNA. The previously characterized invariant delta, epsilon, and gamma chains of the TCR, referred to as the CD3 complex, share significant sequence and structural homology with each other and are all located within 300 kilobases of each other on human chromosome 11 (11q23). zeta has no sequence similarity to the CD3 chains and the localization of the human zeta gene to the centromeric region of chromosome 1 underscores the fact that it is a distinct genetic component of the TCR.
Subject(s)
Antigens, Differentiation, T-Lymphocyte/analysis , Chromosome Mapping , Cloning, Molecular , Membrane Glycoproteins/genetics , Membrane Proteins , Receptors, Antigen, T-Cell/analysis , Receptors, Antigen, T-Cell/genetics , Amino Acid Sequence , Antigens, Differentiation, T-Lymphocyte/genetics , Base Sequence , CD3 Complex , Chromosome Banding , Chromosomes, Human, Pair 11 , DNA/analysis , Humans , Molecular Sequence Data , Nucleic Acid HybridizationABSTRACT
The effects of lesions of the suprachiasmatic nucleus (SCN) on the circadian rhythms in melatonin and cortisol were examined in the rhesus monkey. The concentrations of the two hormones were monitored in cerebrospinal fluid (CSF) withdrawn from two sham-operated animals, two animals with complete bilateral SCN lesions, and two animals with partial SCN damage at 4 and 8 months after surgery. In the sham-operated animals, as in the intact animal, the daily melatonin rhythm was entrained to the daily light-dark cycle, was suppressed in constant light, and persisted in constant darkness. In contrast, neither animal with complete SCN ablation exhibited a daily pattern of CSF melatonin in diurnal lighting at 4 months after surgery nor were their melatonin levels at constant low values. Furthermore, CSF melatonin concentrations were not suppressed in either animal by constant light. Surprisingly, at 8 months after surgery, spectral analysis revealed a 24-hr component to the melatonin patterns for each animal with complete SCN ablation in both diurnal lighting and constant darkness. The two animals with partial SCN damage exhibited a daily melatonin rhythm in diurnal lighting, but constant light did not suppress CSF melatonin concentrations consistently. Daily rhythms persisted in both for a 6 1/2-d period of study in constant darkness. In contrast to the alterations in the melatonin rhythm after SCN damage, there was no apparent effect of either partial or complete SCN ablation on the daily CSF cortisol rhythm. These data indicate that, in the rhesus monkey, the SCN is important for the generation, photic entrainment, and photic suppression of the melatonin rhythm. However, circadian oscillators located outside of the SCN region may control the normal daily cortisol rhythm and perhaps the melatonin rhythm in the absence of the SCN.
Subject(s)
Circadian Rhythm , Hydrocortisone/cerebrospinal fluid , Hypothalamus/physiology , Melatonin/cerebrospinal fluid , Supraoptic Nucleus/physiology , Amino Acids/metabolism , Animals , Autoradiography , Macaca mulatta , Male , Supraoptic Nucleus/cytology , TritiumABSTRACT
The effects of alterations in environmental lighting on the daily rhythm in cerebrospinal fluid concentrations of melatonin were studied in the rhesus monkey. It was found that acute exposure to darkness during the day did not markedly increase normally low daytime CSF melatonin levels, that light suppressed the normally high CSF melatonin values at night, and that 12-h phase shifts in the diurnal lighting cycle caused 12-h phase shifts in the rhythm. The daily rhythm persisted for 6.5 days of study in constant darkness and the phase of the rhythm was not affected in constant darkness by a 12-h phase shift in the daily delivery of food and daily care of the animals. These results support the notion that the melatonin rhythm in this primate species is endogenous in nature, and that light can act to both coordinate the rhythm to the 24-h day and to acutely suppress melatonin production.
Subject(s)
Circadian Rhythm , Melatonin/cerebrospinal fluid , Animals , Darkness , Light , Lighting , Macaca mulatta , MicroclimateABSTRACT
The ontogeny of the pineal melatonin rhythm was determined in three rodent species. Pineal glands were obtained either during the day or during the expected peak in melatonin at night. In all species the rhythm was first detected during the second week of life. Investigations of the photic regulation of pineal melatonin revealed that light could inhibit the nocturnal increase in melatonin in all species by the end of the second week of life. These studies indicate that for these species the development of the rhythm in pineal melatonin and the development of the mechanism involved in the photic suppression of the nocturnal increase in melatonin are independent of the role the pineal gland plays in regulating reproductive function in response to changes in environmental lighting.