ABSTRACT
BACKGROUND: Femtosecond laser-assisted in situ keratomileusis (LASIK) has proven to be an efficacious, predictable, and safe procedure for the correction of refractive errors. We examined the early tear protein changes of patients undergoing LASIK surgery in order to better understand the mechanisms and proteins related to laser corneal surgery and initial recovery. METHODS: Corneal flaps were created with Ziemer FEMTO LDV Z6 I femtosecond laser and stroma was ablated using Wavelight EX500 excimer laser. Tear samples were collected preoperatively as well as 1.5 h and 1 month after LASIK treatment using glass microcapillary tubes. Relative quantification of tear proteins was performed with sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS). RESULTS: SWATH-MS revealed that 158 proteins had altered expression levels 1.5 h after the operation. Two-thirds of these proteins, mostly connected to migration and inflammation response, returned to preoperative levels within the first postoperative month. The other proteins, which did not return to baseline levels, included proteins connected to for example epithelial barrier function. We also identified several proteins, which correlated with surgical variables, such as the amount of correction, flap thickness and flap diameter. CONCLUSIONS: The present study showed that an uneventful femtosecond LASIK refractive surgery induced a significant immune cell migration and inflammation-associated changes in tear proteomics profile quickly after the operation, but the expression of most proteins recovered almost completely to the preoperative levels within the first month. The individual proteins identified in our study are potential targets for the follow-up and modification of LASIK-induced biochemical processes.
ABSTRACT
Measurement of nonlinear optical reflectivity of saturable absorber devices is discussed. A setup is described that enables absolute accuracy of reflectivity measurements better than 0.3%. A repeatability within 0.02% is shown for saturable absorbers with few-percent modulation depth. The setup incorporates an in situ knife-edge characterization of beam diameters, making absolute reflectivity estimations and determination of saturation fluences significantly more reliable. Additionally, several measures are discussed to substantially improve the reliability of the reflectivity measurements. At its core, the scheme exploits the limits of state-of-the-art digital lock-in technology but also greatly benefits from a fiber-based master-oscillator power-amplifier source, the use of an integrating sphere, and simultaneous comparison with a linear reflectivity standard.
ABSTRACT
A picosecond GaInP/AlGaInP/GaAs vertical external-cavity surface-emitting laser (VECSEL) at 675 nm is reported. The laser is mode-locked with a GaInP/AlGaInP/GaAs saturable absorber mirror and emitted ~5.1 ps pulses at a 973 MHz repetition rate and an average power of 45 mW. To our knowledge, this is the first demonstration of a passively mode-locked VECSEL emitting fundamental laser radiation at the visible part of the spectrum.
ABSTRACT
We report on optically-pumped vertical-external-cavity surface-emitting lasers passively mode-locked with a semiconductor saturable-absorber mirror. The potential of harmonic mode-locking in producing pulse trains at multigigahertz repetition rates has been explored. The results present first systematic study of multiple pulse formation in passively mode-locked VECSELs.
ABSTRACT
We report on an optically-pumped intracavity frequency doubled GaInNAs/GaAs -based semiconductor disk laser emitting around 615 nm. The laser operates at fundamental wavelength of 1230 nm and incorporates a BBO crystal for light conversion to the red wavelength. Maximum output power of 172 mW at 615 nm was achieved from a single output. Combined power from two outputs was 320 mW. The wavelength of visible emission could be tuned by 4.5 nm using a thin glass etalon inside the cavity.
ABSTRACT
The behavior of transient oscillations has been studied experimentally for the first time in a broadly tunable ytterbium fiber laser. Spectroscopic study of the relaxation frequency allows one to distinguish three- and four-level transitions and provides a useful tool for controlling the dynamics of pulsed lasers. Particularly, the relaxation oscillation frequency depends on the occupation of the terminal level of the laser transition and clearly shows that the laser transition becomes four-level at the long-wavelength tail of the gain spectrum of ytterbium fiber (lambda > 1060 nm). The wavelength dependence of relaxation oscillations can be used to determine the parameters of the gain material such as transition crosssection.
ABSTRACT
A compact fiber laser is demonstrated with use of a Gires-Tournois compensator and a short length (2-4 cm-long) of highly doped ytterbium (Yb) fiber providing net anomalous group-velocity dispersion. With use of a novel semiconductor saturable absorber mirror based on GaInNAs structure, self-started 1.5-ps-pulse mode-locked operation was obtained at 1023 nm with a repetition rate of 95 MHz. A mode-locked Yb-doped fiber laser was developed without the use of any dispersion compensation technique. Overall group-velocity dispersion was minimized by using a short length of highly doped Yb fiber in a compact amplifying loop cavity. Self-started mode-locked operation was obtained in 980-1030-nm wavelength range with a fundamental repetition rate of 140 MHz.
