ABSTRACT
We studied morphological changes in the prostate ventral lobe, proliferative activity of the epithelium in prostate acini, and the levels of prolactin and prostate-specific antigen in the blood serum of Sprague-Dawley rats after repeated injections of sulpiride in a dose of 40 mg/ kg over 30 and 60 days and in 10 and 30 days after withdrawal. Morphological and morphometrical analysis of hyperplastic changes in the prostate ventral lobe was performed. Ki-67+ proliferating epithelial cells in the acini were counted. The dynamics of serum concentrations of prolactin and prostate-specific antigen was evaluated by ELISA. Morphological and morphometrical analysis and evaluation of the content of Ki-67+ cells demonstrated epithelium hyperplasia in the prostate ventral lobe after sulpiride treatment for 30 or 60 days and in 10 days after withdrawal, but serum level of prostate-specific antigen did not differ from the control. After 60-day sulpiride treatment and in 30 days after withdrawal, pronounced hyperplastic changes of prostate and elevated concentrations of prostate-specific antigen (but not prolactin) were observed. Thus, administration of sulpiride (40 mg/kg) to Sprague-Dawley rats for 60 days allows, by morphological criteria and serum level of prostate-specific antigen, to model stable hyperplastic changes in the prostate corresponding to benign prostatic hyperplasia in humans.
Subject(s)
Dopamine Antagonists/administration & dosage , Prolactin/genetics , Prostate-Specific Antigen/genetics , Prostate/drug effects , Prostatic Hyperplasia/pathology , Sulpiride/administration & dosage , Acinar Cells/drug effects , Acinar Cells/metabolism , Acinar Cells/pathology , Animals , Disease Models, Animal , Epithelial Cells/drug effects , Epithelial Cells/metabolism , Epithelial Cells/pathology , Gene Expression , Humans , Injections, Intramuscular , Ki-67 Antigen/genetics , Ki-67 Antigen/metabolism , Male , Prolactin/blood , Prostate/metabolism , Prostate/pathology , Prostate-Specific Antigen/blood , Prostatic Hyperplasia/blood , Prostatic Hyperplasia/chemically induced , Prostatic Hyperplasia/genetics , Rats , Rats, Sprague-Dawley , Testosterone/bloodABSTRACT
Our research with Sprague-Dawley rats demonstrates protective properties of recombinant human heat shock protein 70 kDa (exogenous rhHSP70) as a prevent therapy agent for gram-positive sepsis. In this study we investigate acute toxicity of rhHSP70 on CD-1 mice and demonstrate very low dangerous of the substance.
Subject(s)
HSP70 Heat-Shock Proteins/therapeutic use , Recombinant Proteins/therapeutic use , Sepsis/prevention & control , Animals , Disease Models, Animal , Humans , Lipopolysaccharides/immunology , Male , Mice , Mice, Inbred Strains , Rats , Rats, Sprague-Dawley , Staphylococcus aureus/immunology , Teichoic Acids/immunologySubject(s)
HSP70 Heat-Shock Proteins/therapeutic use , Recombinant Proteins/therapeutic use , Sepsis/drug therapy , Staphylococcal Infections/drug therapy , Staphylococcus aureus , Animals , Disease Models, Animal , HSP70 Heat-Shock Proteins/genetics , Humans , Lipopolysaccharides/immunology , Male , Rats , Rats, Sprague-Dawley , Recombinant Proteins/genetics , Teichoic Acids/immunologyABSTRACT
The protective effects of 70-kDa heat shock proteins (HSP70) were studied following the intravenous administration in rats with endotoxic shock. The antitoxic effects of both bovine HSP70 (bHSP70) and human recombinant HSP70 (hrHSP70) were compared. The preventive uptake of HSP70 decreased the toxic influence of the E. coll endotoxin on the rats' bodies and significantly increased the survival of the animals during the experiment.
