ABSTRACT
Enteroparasitosis is a public health problem in Brazil. Clinical indications and the appropriate stool examination are essential to obtain an adequate result. This study aims to evaluate whether the clinical indications and the choice of coproparasitological tests requested by the medical services may influence the diagnosis of enteroparasitosis. The data was obtained from the records in the Laboratory of Parasitology at the Pedro Ernesto University Hospital (HUPE/ UERJ) of the State University of Rio de Janeiro (UERJ) from 2009 to 2014. The qualitative variables were grouped in medical services (medical surgery, infectious and parasitic diseases, gastroenterology, pediatrics and rheumatology); types of tests requested (parasitological stool examination (PSE), merthiolate-iodine-formaldehyde (MIF), and sodium-acetate acetic acidformaldehyde (SAF)) and clinical indications (anemia, diarrhea, abdominal pain, eosinophilia, routine tests, HTLV patients, HIV patients, parasitosis and transplantation research). The chi square (X²) and the Spearman coefficient correlation tests were performed to calculate the association between the clinical indications and the coproparasitological tests. A significant association was evident in the clinical indication: parasitosis found among the MIF tests and Trichrome Wheatley (ρ = 0.980). In other clinical indications such as anemia, surgery/ transplant, diarrhea, patients with HIV, HTLV and eosinophilia (despite the PSE tests and MIF having presented a strong link (ρ = 0.802), there was no significant association among the tests. Clinical indications are essential and they have a great influence on the parasitological diagnosis, requiring a combination of diagnostic methods for the detection of protozoa and helminths of medical interest.
Subject(s)
Parasitic Diseases , Parasitology , Public HealthABSTRACT
Acanthamoeba polyphaga is a free-living protozoan pathogen, whose infective trophozoite form is capable of causing a blinding keratitis and fatal granulomatous encephalitis in humans. The damage caused by A. polyphaga trophozoites in human corneal or brain infections is the result of several different pathogenic mechanisms that have not yet been elucidated at the molecular level. We performed a comprehensive analysis of the proteins expressed by A. polyphaga trophozoites, based on complementary 2-DE MS/MS and gel-free LC-MS/MS approaches. Overall, 202 non-redundant proteins were identified. An A. polyphaga proteomic map in the pH range 3-10 was produced, with protein identification for 184 of 370 resolved spots, corresponding to 142 proteins. Additionally, 94 proteins were identified by gel-free LC-MS/MS. Functional classification revealed several proteins with potential importance for pathogen survival and infection of mammalian hosts, including surface proteins and proteins related to defense mechanisms. Our study provided the first comprehensive proteomic survey of the trophozoite infective stage of an Acanthamoeba species, and established foundations for prospective, comparative and functional studies of proteins involved in mechanisms of survival, development, and pathogenicity in A. polyphaga and other pathogenic amoebae.