Perfusion Decellularization of Extrahepatic Bile Duct Allows Tissue-Engineered Scaffold Generation by Preserving Matrix Architecture and Cytocompatibility.

Reconstruction of bile ducts damaged remains a vexing medical problem. Surgeons have few options when it comes to a long segment reconstruction of the bile duct. Biological scaffolds of decellularized biliary origin may offer an approach to support the replace of bile ducts. Our objective was to obtain an extracellular matrix scaffold derived from porcine extrahepatic bile ducts (dECM-BD) and to analyze its biological and biochemical properties. The efficiency of the tailored perfusion decellularization process was assessed through histology stainings. Results from 4'-6-diamidino-2-phenylindole (DAPI), Hematoxylin and Eosin (H&E) stainings, and deoxyribonucleic acid (DNA) quantification showed proper extracellular matrix (ECM) decellularization with an effectiveness of 98%. Immunohistochemistry results indicate an effective decrease in immunogenic marker as human leukocyte antigens (HLA-A) and Cytokeratin 7 (CK7) proteins. The ECM of the bile duct was preserved according to Masson and Herovici stainings. Data derived from scanning electron microscopy (SEM) and thermogravimetric analysis (TGA) showed the preservation of the dECM-BD hierarchical structures. Cytotoxicity of dECM-BD was null, with cells able to infiltrate the scaffold. In this work, we standardized a decellularization method that allows one to obtain a natural bile duct scaffold with hierarchical ultrastructure preservation and adequate cytocompatibility.

Evaluación radiográfica de implantes de zinalco en fémur de perros / Radiographic evaluation of zinalco implants (80 percent Zn - 18 percent Al - 2 percent Cu), zinalco coated with titanium nitride and stainles steel 316L in dogs femur

Este trabajo es la búsqueda de nuevas alternativas para probar materiales con aplicaciones médicas. Se emplearon cuatro perros mestizos a los que se les implantaron cilindros de zinalco y acero 3l6L en los tercios proximal y distal del fémur izquierdo, respectivamente. El propósito fue evaluar la biocompatibilidad de estos materiales desde el punto de vista clínico, para lo cual se llevó a cabo el estudio radiográfico previo y posterior a la cirugía a los 30, 60, 90 y 120 días, evaluándose la densidad ósea, el rechazo al implante, signos de metalosis y reacción perióstica. En todos los casos hubo formación de hueso adyacente a los implantes, observada radiográficamente por reacción perióstica laminar, endostitis y esclerosis del canal medular. No se observaron reacciones periósticas severas e irregulares ni reabsorción ósea. No hubo evidencias de rechazo en los perros implantados a los 120 días de implantación de los materiales evaluados