Single-Cell Phenotyping of Extracellular Electron Transfer via Microdroplet Encapsulation.

Electroactive organisms contribute to metal cycling, pollutant removal, and other redox-driven environmental processes. Studying this phenomenon in high-throughput is challenging since extracellular reduction cannot easily be traced back to its cell of origin within a mixed population. Here, we describe the development of a microdroplet emulsion system to enrich EET-capable organisms. We validated our system using the model electroactive organism S. oneidensis and describe the tooling of a benchtop microfluidic system for oxygen-limited processes. We demonstrated enrichment of EET-capable phenotypes from a mixed wild-type and EET-knockout population. As a proof-of-concept application, bacteria were collected from iron sedimentation from Town Lake (Austin, TX) and subjected to microdroplet enrichment. We observed an increase in EET-capable organisms in the sorted population that was distinct when compared to a population enriched in a bulk culture more closely akin to traditional techniques for discovering EET-capable bacteria. Finally, two bacterial species, C. sakazakii and V. fessus not previously shown to be electroactive, were further cultured and characterized for their ability to reduce channel conductance in an organic electrochemical transistor (OECT) and to reduce soluble Fe(III). We characterized two bacterial species not previously shown to exhibit electrogenic behavior. Our results demonstrate the utility of a microdroplet emulsions for identifying putative EET-capable bacteria and how this technology can be leveraged in tandem with existing methods.

Risk of lead exposure from wild game consumption from cross-sectional studies in Madre de Dios, Peru.

Background: Studies have shown elevated blood lead levels (BLL) in residents of remote communities in the Amazon, yet sources of lead exposure are not fully understood, such as lead ammunition consumed in wild game. Methods: Data was collected during two cross-sectional studies that enrolled 307 individuals in 26 communities. Regression models with community random effects were used to evaluate risk factors for BLLs, including diet, water source, smoking, sex, age, and indigenous status. The All-Ages Lead Model (AALM) from the Environmental Protection Agency (EPA) was used to estimate background and dose from wild game consumption. Findings: Indigenous status and wild game consumption were associated with increased BLLs. Indigenous participants had 2.52 µg/dL (95% CI: 1.95-3.24) higher BLLs compared to non-indigenous. Eating wild game was associated with a 1.41 µg/dL (95% CI: 1.20-1.70) increase in BLLs. Two or more portions per serving were associated with increased BLLs of 1.66 µg/dL (95% CI: 1.10-2.57), compared to smaller servings. Using the AALM, we estimate background lead exposures to be 20 µg/day with consumption of wild game contributing 500 µg/meal. Lastly, we found a strong association between BLLs and mercury exposure. Interpretation: Consumption of wild game hunted with lead ammunition may pose a common source of lead exposure in the Amazon. Communities that rely on wild game and wild fish may face a dual burden of exposure to lead and mercury, respectively.

Development of a reproducible method for monitoring SARS-CoV-2 in wastewater.

Monitoring the genetic signal of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) through RNA titers in wastewater has emerged as a promising strategy for tracking community-scale prevalence of coronavirus disease 2019 (COVID-19). Although many studies of SARS-CoV-2 in wastewater have been conducted around the world, a uniform procedure for concentrating the virus in wastewater is lacking. The goal of this study was to comprehensively evaluate how different methods for concentrating the suspended solids in wastewater affect the associated SARS-CoV-2 RNA signal and the time required for processing samples for wastewater-based epidemiology efforts. We additionally consider the effects of sampling location in the wastewater treatment train (i.e., following preliminary or primary treatment), pasteurization, and RNA extraction method. Comparison of the liquid phase to suspended solids obtained via centrifugation or vacuum filtration suggests that the RNA signal of SARS-CoV-2 preferentially occurs in the solids. Therefore, we assert that the recovery of SARS-CoV-2 from wastewater should focus on suspended solids. Our data indicate that the measured SARS-CoV-2 signal is higher among samples taken from the primary clarifier effluent, as opposed to those taken after preliminary treatment. Additionally, we provide evidence that sample pasteurization at 60 °C for 90 min reduces the SARS-CoV-2 signal by approximately 50-55%. Finally, the results indicate that a magnetic bead approach to RNA extraction leads to a higher SARS-CoV-2 signal than does a silica membrane approach.

Implicit theories of online trolling: Evidence that attention-seeking conceptions are associated with increased psychological resilience.

Three studies were conducted to investigate people's conceptions of online trolls, particularly conceptions associated with psychological resilience to trolling. In Study 1, a factor analysis of participants' ratings of characteristics of online trolls found a replicable bifactor model of conceptions of online trolls, with a general factor of general conceptions towards online trolls being identified, but five group factors (attention-conflict seeking, low self-confidence, viciousness, uneducated, amusement) as most salient. In Study 2, participants evaluated hypothetical profiles of online trolling messages to establish the validity of the five factors. Three constructs (attention-conflict seeking, viciousness, and uneducated) were actively employed when people considered profiles of online trolling scenarios. Study 3 introduced a 20-item 'Conceptions of Online Trolls scale' to examine the extent to which the five group factors were associated with resilience to trolling. Results indicated that viewing online trolls as seeking conflict or attention was associated with a decrease in individuals' negative affect around previous trolling incidents. Overall, the findings suggest that adopting an implicit theories approach can further our understanding and measurement of conceptions towards trolling through the identification of five salient factors, of which at least one factor may act as a resilience strategy.

An evaluation of the EQUIP treatment programme with men who have intellectual or other developmental disabilities.

BACKGROUND: The Equipping Youth to Help One Another Programme (EQUIP) was designed for young offenders to address a developmental delay in moral reasoning, distorted cognitions and social skills. METHODS: The present authors undertook a single case series study and piloted an adapted version of the EQUIP programme with three men with intellectual disabilities and four men with a diagnosis of Asperger Syndrome, all of whom were detained in a medium-secure forensic unit for people with intellectual disabilities. Treatment was delivered over a 12-week period, and participants took part in four-one-hour sessions per week. RESULTS: The results suggested that treatment was successful at increasing moral reasoning ability, reducing distorted cognitions and improving ability to choose effective solutions to problems. However, treatment did not have a significant effect upon anger. CONCLUSIONS: The EQUIP programme is a promising treatment, but further research is needed to investigate its effectiveness with men with intellectual or other developmental disabilities.

Validation of an adaptation of Levenson's locus of control scale with adult male incarcerated sexual offenders.

This article examines the psychometric properties of an adaptation of Levenson's Locus of Control (LoC) measure that is used by the English and Welsh Prison Service as part of the psychometric assessment battery for sexual offenders participating in the Sex Offender Treatment Programme (SOTP). Reliability and validity analyses were conducted on a sample of 2,497 sexual offenders who had completed SOTP. Internal consistency, convergent validity, and relationship to socially desirable responding were investigated. Construct validity was assessed via exploratory factor analysis (EFA) and confirmatory factor analysis (CFA). Test-retest reliability data were collected from an additional sample of 26 sexual offenders. The scale was found to have excellent internal consistency, good test-retest reliability, and weak to moderate convergent validity with measures of self-esteem, attachment styles, emotional loneliness, and social problem solving. There was a moderate correlation between the scale and socially desirable responding. EFA and CFA suggested that a four-factor solution provided an acceptable fit to the data, with the factors relating to constructs of chance, problem solving, powerful others, and internal control. The findings of the present study suggest that the adapted LoC Scale is a useful tool for assessing sexual offenders' locus of control.

The effect on reconviction of an intervention for drink-driving offenders in the community.

This study reports an evaluation of the Drink-Impaired Drivers program in the English and Welsh probation service. Participants were adult male offenders who had been convicted of a drink-driving offence and were serving community sentences. The 1-year drink-drive reconviction rates were compared for offenders who completed the program, offenders who started but did not complete the program, and a comparison group who were not allocated to the program. At 1-year follow-up, there was no reconviction among offenders who had completed the program. Multivariate analysis showed that the noncompleters had a significantly higher rate of reconviction than the completers and comparison group.

Methodological considerations in the evaluation of offender interventions: the problem of attrition.

Attrition from offender interventions presents methodological problems when the effectiveness of the intervention is under evaluation. This article proposes a treatment-received (TR) design, which incorporates one-to-one matching on criminogenic variables. This design permits the comparison of completer, noncompleter, and nonstarter groups with their paired comparisons and, hence, allows a more accurate evaluation of program completion and noncompletion effects. A sample of 173 offenders, referred to one of two general offending behavior cognitive skills programs within community settings, was matched one-to-one by criminogenic variables to offenders from an appropriate comparison group. Reconviction analyses were undertaken using intention to treat (ITT) analysis, TR analysis using the full comparison group, and TR analysis using matched comparison groups. The ITT design found no impact of group membership on reconviction. The TR designs, however, provided evidence of moderate effects of completion: For every three completers, five of the matched comparison group members were reconvicted. The analysis also suggested a noncompletion effect: Noncompleters were twice as likely as their matched comparison group to be reconvicted.

Relationships among moral reasoning, empathy, and distorted cognitions in men with intellectual disabilities and a history of criminal offending.

Eighty men, spread equally across 4 groups, were recruited, including men with and without intellectual disabilities. The men were either criminal offenders or nonoffenders. Participants completed measures of moral reasoning, empathy, and distorted cognitions. The results indicated that the moral reasoning abilities of offenders with intellectual disabilities were developmentally delayed but were still more mature than those of nonoffenders with intellectual disabilities. Offenders without intellectual disabilities had less mature moral reasoning abilities than nonoffenders without intellectual disabilities. The differences may be partially accounted for by intellectual ability. The results also indicated that the relationship between empathy and distorted cognitions was mediated by moral reasoning. The findings have implications for the use of psychological interventions with offenders with intellectual disabilities.

The psychometric properties of the Socio-Moral Reflection Measure--Short Form and the Moral Theme Inventory for men with and without intellectual disabilities.

Drawing conclusions from the literature regarding the moral development of people with intellectual disabilities (IDs) is difficult because of the use of unstandardised and idiosyncratic measures. In order to address this short-coming, a moral reasoning production measure (the Socio-Moral Reflection Measure--Short Form; SRM-SF) and a recognition measure (the Moral Theme Inventory; MTI) were presented to men with and without IDs who had no known history of engaging in illegal behaviour. The instruments were completed on two occasions, separated by a two-week interval, in order to investigate their basic psychometric properties. The results indicated that there was a strong relationship between the MTI and the SRM-SF, suggesting that the MTI has convergent validity. The internal consistency of the MTI and the SRM-SF ranged from moderate to substantial for both men with and without IDs. However, the test-retest reliability of the MTI was poor for men with IDs, while it was good for men without IDs. The test-retest reliability of the SRM-SF was good for both men with and without IDs. Comparison of the moral reasoning abilities of men with and without IDs suggested that many of the differences between the two groups could be accounted for by general intellectual functioning. The exception was overall score on the SRM-SF and moral reasoning in relation to the law, where men with IDs scored at stage 2(1), when intelligence was controlled. The results were interpreted by suggesting that the relationship between moral reasoning and illegal behaviour may take an inverted U curve shape, moderated by intelligence.

Substance use, mental health, and relationships: A comparison of male and female offenders serving community sentences.

This study examined criminogenic need in the areas of drug use, alcohol use, mental health, and relationships, in 6453 male and 1045 female probationers, using data from the Offender Assessment System (OASys). It also examined the associations between these four areas. Females had significantly higher levels of need than males for mental health and relationships, and males had significantly higher levels of need for alcohol use. There was no group difference for drug use. For both males and females, there were significant positive correlations for scores between all pairs of needs, except for drug and alcohol use. The strength of the association between relationships and drug use was significantly stronger among females as compared to males. The results are discussed in light of the previous research and their implications for interventions to reduce offending.

Psychometric properties of the Beck Depression Inventory-II with incarcerated male offenders aged 18-21 years.

BACKGROUND: The Beck Depression Inventory - Second Edition (BDI-II) is a self-report measure of depression. Studies have shown it to have good psychometric properties with adult and adolescent clinical and non-clinical populations. However, this research has mostly been conducted with North American samples. AIMS/HYPOTHESES: To examine the psychometric characteristics of the BDI-II with male young adult offenders in the UK. METHODS: The BDI-II was administered to 117 incarcerated male young adult offenders aged 18-21 years from the UK. RESULTS: The BDI-II showed good internal consistency and concurrent validity. Factor analysis revealed two factors, relating to cognitive-affective items and somatic items. The items loading on the two factors were very similar to those found in a North American adolescent (13-17 years) psychiatric inpatient sample. CONCLUSIONS AND IMPLICATIONS FOR FUTURE RESEARCH: The findings suggest that the BDI-II can be used with confidence in young adult male offenders. It would be useful to confirm its psychometric properties in other offender samples and establish offender population norms.

The importance of appropriate allocation to offending behavior programs.

This study examined the impact on reconviction of appropriate allocation to three general offending behavior programs involving adult male offenders in the English and Welsh Probation Service. Appropriate allocation was defined by level of risk for reconviction. There were three allocation groups: too low, appropriate, and too high. Using a quasi-experimental design, the reconviction rates of offenders who were allocated to and completed a program, offenders allocated to a program who failed to start, and a comparison group were compared. It was found that the appropriateness of allocation affected reconviction independently of treatment group. Furthermore, in line with the risk principle, there was an interaction between treatment group and the appropriateness of allocation.

The relationship between moral reasoning, provictim attitudes, and interpersonal aggression among imprisoned young offenders.

This study examined the relationship between moral reasoning, provictim attitudes, and interpersonal aggression among imprisoned young offenders. The participants were 60 imprisoned male young offenders from a young offender institution or remand centre. Using the Direct and Indirect Prisoner Behaviour Checklist, the offenders were categorised as one of four groups: perpetrator, victim, perpetrator-victim, or not involved. Participants in the four groups were compared on measures of provictim attitudes and sociomoral reasoning. The results showed that the majority of the participants were involved in victimising behaviours, with 43.3% falling into the perpetrator-victim category. Victims had significantly more empathic attitudes toward victims than did those in the perpetrator-victim group. No significant differences were found among the four groups on the sociomoral reasoning measure, nor was there a significant correlation between sociomoral reasoning and provictim attitudes. The results are discussed in terms of previous research and their implications for practice.

Depression, hopelessness and suicide ideation among vulnerable prisoners.

BACKGROUND: Self-harm among prisoners is high, and suicide rates increasing. Assessment of depressive characteristics is easy. To what extent are these linked with previous self-harm? AIMS: To compare depressive characteristics of prisoners who report previous self-harm with those who do not. METHODS: Twenty-four new arrivals at an adult male category B local prison who reported previous episodes of suicidal behaviour (including self-harm and/or explicit attempted suicide) were assessed using the Beck Hopelessness Scale, the Beck Depression Inventory-II, and the Beck Scale for Suicide Ideation. A further 24 new arrivals were matched as closely as possible with them on sociodemographic and offending characteristics. RESULTS: Mean scores on the Beck Hopelessness Scale, the Beck Depression Inventory-II, and the Beck Scale for Suicide Ideation were significantly higher among the prisoners with a history of self-harm. DISCUSSION: Prisoners with a previous history of self-harm are more likely than those without to show a range of depressive symptoms than their imprisoned peers without such a history, suggesting a continued vulnerability to self-harm and perhaps suicide.

Level of Service Inventory-Revised profiles of violent and nonviolent prisoners.

Level of Service Inventory-Revised (LSI-R) assessments were conducted on 251 male prisoners beginning prison sentences in six English prisons. The prisoners who were classified as violent, on the basis of either current or previous convictions, scored significantly higher than the nonviolent prisoners on the four LSI-R subscales of Criminal History, Companions, Education and Employment, and Alcohol and Drugs. In addition, those prisoners with a record of violent offenses gave higher total LSI-R scores, indicating an overall higher risk of recidivism. Multivariate analysis using the LSI-R subscales to classify the higher risk violent prisoners and minimum-risk nonviolent prisoners basically confirmed the findings of the univariate analysis. The implications of the findings for informing practice with violent offenders are discussed in terms of screening for intensity of service delivery and identification of targets for change. It is suggested that screening for drug and alcohol problems should be a prerequisite to working with violent offenders.

Victimizing behaviour among juvenile and young offenders: how different are perpetrators?

This study examined the victimizing behaviours of incarcerated juvenile and young offenders. A total of 108 juveniles and young offenders completed questionnaires relating to victimizing behaviours and were administered the Custodial Adjustment Questionnaire (CAQ: Thornton, 1987 In Applying Psychology to Imprisonment: Theory and Practice, McGurk B.J., Thornton, D. and Williams, M. (Eds). London: HMSO, pp. 445-465) and Hostility and Direction of Hostility Questionnaire (HDHQ: Caine et al., 1967. Manual of the Hostility and Direction of Hostility Questionnaire. London: University of London press). It was found that 50.9% of all respondents reported victimizing others, with verbal assaults and threats being the most common form of such behaviours. Furthermore, staff-identified "victimizers" were significantly more likely to report victimizing behaviour than the rest of the sample. Those who had been in custody longer were more likely to report victimizing others, as were younger offenders (15-17-year olds as compared to 18-21-year olds). Victimizers were also more likely to report having previously experienced victimization themselves. On the psychometric measures, self-reported victimizers scored significantly higher on the deviance sub-scale of the CAQ, and on a number of the sub-scales of the HDHQ. These findings are discussed in terms of previous literature and their practical implications considered.