ABSTRACT
OBJECTIVE: To explore the effect of comprehensive nursing on negative emotion and prognosis of patients with sepsis. METHODS: As a prospective study, 104 patients with sepsis were randomized into the observation group (n=52) and the control group (n=52). The patients in the control group underwent routine nursing, whereas the patients in the observation group underwent comprehensive nursing care in addition to routine nursing. The level of negative emotions, patients' prognosis, quality of life (QOL), Acute Physiology and Chronic Health Evaluation (APACHE) ll score, clinical indicators, and patient satisfaction with nursing were compared between the two groups. RESULTS: Compared with the control group, the observation group had lower Self-Rating Anxiety Scale score, Self-Rating Depression Scale score, and APACHE ll score (all P<0.001). The scores of physical functioning, general health perceptions, social role functioning, emotional role functioning, and mental health of the observation group were all higher than those of the control group (all P<0.01). The duration of mechanical ventilation, hospitalization expenses, and the length of stay in the Intensive Care Unit (ICU) in the observation group were lower than those in the control group (all P<0.01). Moreover, the observation group had a lower total incidence of shock, multiple organ dysfunction syndrome, and death and higher patient satisfaction with the nursing care than the control group (all P<0.05). CONCLUSION: Comprehensive nursing care can alleviate anxiety and depression in patients with sepsis and can improve the prognosis and QOL of patients. Also, it can shorten the length of stay in the ICU, lower treatment costs, and improve patient satisfaction; all of which can be recommended for clinical application.
ABSTRACT
OBJECTIVE: Emerging evidence has revealed that exosomal microRNAs (miRNAs) are implicated in human diseases. However, role of exosomal miR-125b-5p in sepsis-induced acute lung injury (ALI) remains further explored. We focused on the effect of exosomal miR-125b-5p on ALI progression via targeting topoisomerase II alpha (TOP2A). METHODS: The ALI mouse models were established by cecal ligation and perforation, which were then treated with miR-125b-5p agomir or overexpressed TOP2A. Next, the pathological structure of ALI mouse lung tissues were observed, miR-125b-5p, TOP2A and vascular endothelial growth factor (VEGF) expression was determined, and the lung water content, inflammatory response, protein content in bronchoalveolar lavage fluid (BALF) and cell apoptosis in ALI mouse lung tissues were assessed. Exosomes were extracted from endothelial cells (ECs) and identified, which were then injected into the modeled mice to observe their roles in ALI. The targeting relationship between miR-125b-5p and TOP2A was confirmed. RESULTS: MiR-125b-5p was downregulated while TOP2A was upregulated in ALI mice. MiR-125b-5p elevation or ECs-derived exosomes promoted VEGF expression, improved pathological changes and restrained lung water content, inflammatory response, protein content in BALF and cell apoptosis in lung tissues ALI mice. TOP2A overexpression reversed the repressive role of miR-125b-5p upregulation in ALI, while downregulated miR-125b-5p abrogated the effect of ECs-derived exosomes on ALI. TOP2A was confirmed as a direct target gene of miR-125b-5p. CONCLUSION: Our study indicates that ECs-derived exosomes overexpressed miR-125b-5p to protect from sepsis-induced ALI by inhibiting TOP2A, which may contribute to ALI therapeutic strategies.