Comparative chloroplast genomics, phylogenetic relationships and molecular markers development of Aglaonema commutatum and seven green cultivars of Aglaonema.

Aglaonema commutatum is a famous species in the Aglaonema genus, which has important ornamental and economic value. However, its chloroplast genome information and phylogenetic relationships among popular green cultivars of Aglaonema in southern China have not been reported. Herein, chloroplast genomes of one variety of A. commutatum and seven green cultivars of Aglaonema, namely, A. commutatum 'San Remo', 'Kai Sa', 'Pattaya Beauty', 'Sapphire', 'Silver Queen', 'Snow White', 'White Gem', and 'White Horse Prince', were sequenced and assembled for comparative analysis and phylogeny. These eight genomes possessed a typical quadripartite structure that consisted of a LSC region (90,799-91,486 bp), an SSC region (20,508-21,137 bp) and a pair of IR regions (26,661-26,750 bp). Each genome contained 112 different genes, comprising 79 protein-coding genes, 29 tRNA genes and 4 rRNA genes. The gene orders, GC contents, codon usage frequency, and IR/SC boundaries were highly conserved among these eight genomes. Long repeats, SSRs, SNPs and indels were analyzed among these eight genomes. Comparative analysis of 15 Aglaonema chloroplast genomes identified 7 highly variable regions, including trnH-GUG-exon1-psbA, trnS-GCU-trnG-UCC-exon1, trnY-GUA-trnE-UUC, psbC-trnS-UGA, trnF-GAA-ndhJ, ccsA-ndhD, and rps15-ycf1-D2. Reconstruction of the phylogenetic trees based on chloroplast genomes, strongly supported that Aglaonema was a sister to Anchomanes, and that the Aglaonema genus was classified into two sister clades including clade I and clade II, which corresponded to two sections, Aglaonema and Chamaecaulon, respectively. One variety and five cultivars, including A. commutatum 'San Remo', 'Kai Sa', 'Pattaya Beauty', 'Silver Queen', 'Snow White', and 'White Horse Prince', were classified into clade I; and the rest of the two cultivars, including 'Sapphire' and 'White Gem', were classified into clade II. Positive selection was observed in 34 protein-coding genes at the level of the amino acid sites among 77 chloroplast genomes of the Araceae family. Based on the highly variable regions and SSRs, 4 DNA markers were developed to differentiate the clade I and clade II in Aglaonema. In conclusion, this study provided chloroplast genomic resources for Aglaonema, which were useful for its classification and phylogeny.

Thirteen complete chloroplast genomes of the costaceae family: insights into genome structure, selective pressure and phylogenetic relationships.

BACKGROUND: Costaceae, commonly known as the spiral ginger family, consists of approximately 120 species distributed in the tropical regions of South America, Africa, and Southeast Asia, of which some species have important ornamental, medicinal and ecological values. Previous studies on the phylogenetic and taxonomic of Costaceae by using nuclear internal transcribed spacer (ITS) and chloroplast genome fragments data had low resolutions. Additionally, the structures, variations and molecular evolution of complete chloroplast genomes in Costaceae still remain unclear. Herein, a total of 13 complete chloroplast genomes of Costaceae including 8 newly sequenced and 5 from the NCBI GenBank database, representing all three distribution regions of this family, were comprehensively analyzed for comparative genomics and phylogenetic relationships. RESULT: The 13 complete chloroplast genomes of Costaceae possessed typical quadripartite structures with lengths from 166,360 to 168,966 bp, comprising a large single copy (LSC, 90,802 - 92,189 bp), a small single copy (SSC, 18,363 - 20,124 bp) and a pair of inverted repeats (IRs, 27,982 - 29,203 bp). These genomes coded 111 - 113 different genes, including 79 protein-coding genes, 4 rRNA genes and 28 - 30 tRNAs genes. The gene orders, gene contents, amino acid frequencies and codon usage within Costaceae were highly conservative, but several variations in intron loss, long repeats, simple sequence repeats (SSRs) and gene expansion on the IR/SC boundaries were also found among these 13 genomes. Comparative genomics within Costaceae identified five highly divergent regions including ndhF, ycf1-D2, ccsA-ndhD, rps15-ycf1-D2 and rpl16-exon2-rpl16-exon1. Five combined DNA regions (ycf1-D2 + ndhF, ccsA-ndhD + rps15-ycf1-D2, rps15-ycf1-D2 + rpl16-exon2-rpl16-exon1, ccsA-ndhD + rpl16-exon2-rpl16-exon1, and ccsA-ndhD + rps15-ycf1-D2 + rpl16-exon2-rpl16-exon1) could be used as potential markers for future phylogenetic analyses and species identification in Costaceae. Positive selection was found in eight protein-coding genes, including cemA, clpP, ndhA, ndhF, petB, psbD, rps12 and ycf1. Maximum likelihood and Bayesian phylogenetic trees using chloroplast genome sequences consistently revealed identical tree topologies with high supports between species of Costaceae. Three clades were divided within Costaceae, including the Asian clade, Costus clade and South American clade. Tapeinochilos was a sister of Hellenia, and Parahellenia was a sister to the cluster of Tapeinochilos + Hellenia with strong support in the Asian clade. The results of molecular dating showed that the crown age of Costaceae was about 30.5 Mya (95% HPD: 14.9 - 49.3 Mya), and then started to diverge into the Costus clade and Asian clade around 23.8 Mya (95% HPD: 10.1 - 41.5 Mya). The Asian clade diverged into Hellenia and Parahellenia at approximately 10.7 Mya (95% HPD: 3.5 - 25.1 Mya). CONCLUSION: The complete chloroplast genomes can resolve the phylogenetic relationships of Costaceae and provide new insights into genome structures, variations and evolution. The identified DNA divergent regions would be useful for species identification and phylogenetic inference in Costaceae.

Comparative Chloroplast Genomics of 21 Species in Zingiberales with Implications for Their Phylogenetic Relationships and Molecular Dating.

Zingiberales includes eight families and more than 2600 species, with many species having important economic and ecological value. However, the backbone phylogenetic relationships of Zingiberales still remain controversial, as demonstrated in previous studies, and molecular dating based on chloroplast genomes has not been comprehensively studied for the whole order. Herein, 22 complete chloroplast genomes from 21 species in Zingiberales were sequenced, assembled, and analyzed. These 22 genomes displayed typical quadripartite structures, which ranged from 161,303 bp to 163,979 bp in length and contained 111-112 different genes. The genome structures, gene contents, simple sequence repeats, long repeats, and codon usage were highly conserved, with slight differences among these genomes. Further comparative analysis of the 111 complete chloroplast genomes of Zingiberales, including 22 newly sequenced ones and the remaining ones from the national center for biotechnology information (NCBI) database, identified three highly divergent regions comprising ccsA, psaC, and psaC-ndhE. Maximum likelihood and Bayesian inference phylogenetic analyses based on chloroplast genome sequences found identical topological structures and identified a strongly supported backbone of phylogenetic relationships. Cannaceae was sister to Marantaceae, forming a clade that was collectively sister to the clade of (Costaceae, Zingiberaceae) with strong support (bootstrap (BS) = 100%, and posterior probability (PP) = 0.99-1.0); Heliconiaceae was sister to the clade of (Lowiaceae, Strelitziaceae), then collectively sister to Musaceae with strong support (BS = 94-100%, and PP = 0.93-1.0); the clade of ((Cannaceae, Marantaceae), (Costaceae, Zingiberaceae)) was sister to the clade of (Musaceae, (Heliconiaceae, (Lowiaceae, Strelitziaceae))) with robust support (BS = 100%, and PP = 1.0). The results of divergence time estimation of Zingiberales indicated that the crown node of Zingiberales occurred approximately 85.0 Mya (95% highest posterior density (HPD) = 81.6-89.3 million years ago (Mya)), with major family-level lineages becoming from 46.8 to 80.5 Mya. These findings proved that chloroplast genomes could contribute to the study of phylogenetic relationships and molecular dating in Zingiberales, as well as provide potential molecular markers for further taxonomic and phylogenetic studies of Zingiberales.

The Longitudinal Relationship between Parent-Child Attachment and Adolescents' Gratitude: The Mediating Role of Perceived Parental Communal Strength.

Previous research has found a significant association between parent-child attachment and gratitude, while the directionality of this association and its potential mechanisms remain unclear. This study examined the longitudinal associations between parent-child attachment, gratitude, and perceived parental communal strength, which is adolescents' perception of their parents' willingness to make sacrifices to provide care and meet their needs. A total of 1348 adolescents and one of their parents (Mage = 12.96, SD = 0.70 years; 51.3% girls) participated in this three-wave study, with a six-month lag. The results of the cross-lagged panel models showed that attachment anxiety and avoidance negatively predicted adolescents' gratitude after six months. Perceived parental communal strength mediated the longitudinal effects of attachment anxiety and avoidance on gratitude. However, the impact of gratitude on perceived parental communal strength and parent-child attachment was non-significant. The findings suggest that adolescents with insecure parent-child attachment have negative perceptions of their parents, which could contribute to their lack of gratitude. Adolescents' negative perceptions of their parents are crucial for understanding the mechanisms underlying the lack of gratitude observed in adolescents with insecure parent-child attachment.

Developing and validating a multidimensional Chinese Parental Psychological Control Scale.

Introduction: This study validated a Chinese Parental Psychological Control Scale (CPPCS) among secondary school students in mainland China. Methods: The item pool consisting of 65 items was constructed based on consultation with existing measures and focus group interviews of 19 Chinese adolescents. After content validation conducted by 14 experts, a total of 40 items were retained and subject to further factorial validation based on a sample of 963 Chinese adolescents (mean age = 13.39 ± 0.72; 52.23% females). Results: Using the two random-split half samples, exploratory and confirmatory factor analyses retained 30 items that loaded on three factors, including "relational induction" (twelve items), "harsh psychological control" (twelve items), and "social comparison shame" (six items). The three-factor structure was invariant across gender (male versus female) and grades (grade 7 versus grade 8) among the whole sample. Cronbach's αs of the three dimensions in maternal and paternal subscales ranged between 0.89 and 0.92, suggesting adequate internal consistency. The three dimensions were significantly correlated with each other, supporting the scale's convergent validity. The concurrent validity of the CPPCS was supported by the positive correlations between subscales and parental rejection, and the negative correlations between subscales and parental warmth. In addition, while the "harsh psychological control" and "social comparison shame" negatively predicted adolescents' well-being, "relational induction" positively predicted adolescents' well-being. Discussion: The findings suggest that the CPPCS is a promising instrument for measuring multidimensional psychological control among Chinese parents and for investigating and comparing individual dimensions' effect on adolescents' development.

The Relationship between Social Participation and Subjective Well-Being among Older People in the Chinese Culture Context: The Mediating Effect of Reciprocity Beliefs.

It is demonstrated that the subjective well-being (SWB) of older people greatly relies on their social participation. However, there are few studies on reciprocity beliefs as a mediating mechanism between social participation and SWB. In this study, 297 participants aged 60 and over in Chengdu, Sichuan Province, China, completed a questionnaire of social participation, reciprocity beliefs, and SWB. We used multiple linear regression and mediation analyses to verify the mediating effect of reciprocity beliefs. Results showed that social participation was positively relative to SWB and reciprocity beliefs, and reciprocity beliefs played an intermediary role in social participation and SWB. These findings suggest the importance of social participation for SWB, with reciprocity beliefs (behaviors) playing a positive mediating role, particularly in China. In conclusion, analysis of the mediating effect of reciprocity beliefs provides us with knowledge that could help in achieving a healthy old age. Additionally, this study opens up new perspectives of research.

Attachment Insecurity and Altruistic Behavior among Chinese Adolescents: Mediating Effect of Different Dimensions of Empathy.

Although Western studies showed that attachment insecurity was negatively related to adolescent altruistic behavior, few studies have investigated this issue among Chinese adolescents, and little is known about the mechanisms underlying the impact of attachment avoidance and attachment anxiety on adolescent altruistic behaviors. This study investigated the mediating role of different dimensions of empathy (empathic concern, perspective taking, and personal distress) on the association of attachment avoidance and attachment anxiety with altruistic behavior among Chinese adolescents. A total of 1005 7th and 8th grade Chinese students (Mage = 12.86 years, SD = 0.69) from three middle schools in Chengdu, China completed measures of attachment insecurity, interpersonal reactivity index, and altruistic behavior. Results indicated that attachment avoidance, not attachment anxiety, negatively predicted adolescent altruistic behavior among Chinese adolescents. Moreover, higher attachment avoidance predicted less empathic concern and perspective taking, which in turn predicted less altruistic behavior, while higher attachment anxiety predicted more empathic concern and personal distress, which further predicted more and less altruistic behavior, respectively. These findings highlight the importance of promoting adolescent empathic concern and perspective taking and reducing personal distress to strengthen adolescent altruistic behavior.

Childhood Maltreatment and Psychosocial Flourishing among Emerging Adults: Roles of Psychological Suzhi and Self-Esteem.

Studies have shown that childhood maltreatment can negatively predict psychosocial flourishing among emerging adults. However, few studies have revealed the factors that can protect the psychosocial flourishing of emerging adults who experienced maltreatment during childhood. Based on theoretical and empirical considerations, this study investigated whether and how psychological suzhi (a positive quality that can facilitate individuals' adaptation to environment) plays a protective role in the relationship between childhood maltreatment and psychosocial flourishing among emerging adults. A total of 2863 Chinese emerging adults (Mage = 19.96 years) completed the self-report measures of the Childhood Trauma Questionnaire, Psychological Suzhi Questionnaire, Rosenberg Self-esteem Scale, and Flourishing Scale. The moderated analyses showed that childhood maltreatment had a less negative impact on psychological flourishing in high psychological suzhi emerging adults than in low psychological suzhi emerging adults. Part of the moderating effect of psychological suzhi is mediated through self-esteem. These results demonstrated that psychological suzhi plays a buffering effect between childhood maltreatment and psychosocial flourishing, and part of the effect is achieved by mediated variable self-esteem. These findings are discussed, and practical implications are presented.

After-School Extracurricular Activities Participation and Depressive Symptoms in Chinese Early Adolescents: Moderating Effect of Gender and Family Economic Status.

Although Western studies showed that participation in extracurricular activities was intimately linked to adolescents' psychological adjustment, very few studies have addressed this issue among early adolescents in China. Based on a nationally representative sample of 9672 Chinese junior high school students (Mage = 14.54 years, SD = 0.70 years), this study investigated the relationship between participation in different extracurricular activities and depressive symptoms among Chinese early adolescents, and the moderating role of gender and family economic status. Results indicated that time spent completing homework, attending extracurricular tutoring, and playing online games after school was positively related to students' depressive symptoms, whereas time spent on participating in physical exercise was negatively associated with students' depressive symptoms. Besides, the relationships between after-school activities participation and student depressive symptoms were moderated by gender and family economic status. The theoretical and practical implications for the arrangement of after-school activities for Chinese early adolescents are discussed.

Participation in After-School Extracurricular Activities and Cognitive Ability Among Early Adolescents in China: Moderating Effects of Gender and Family Economic Status.

Although theories and research suggest that participation in extracurricular activities plays an important role in adolescents' cognitive development, few studies have addressed this issue among early adolescents in China. Based on the responses of 9,830 Chinese junior high school students (M age = 14.54 years, SD = 0.70 years), we investigated the relationships between different types of extracurricular activities and cognitive ability among junior high school students and the moderating effects of gender and family economic status. Using multi-level multiple regression analyses, results indicated that while time spent completing homework and physical exercise was positively associated with students' cognitive ability, time spent on extracurricular tutoring, interest classes, watching TV, and surfing online and playing games was negatively related to students' cognitive ability. The observed relationships were also moderated by gender and family economic status. Specifically, time spent on completing homework had a stronger positive relationship with boys' cognitive ability, whereas time spent attending extracurricular tutoring on weekdays had a stronger negative relationship with girls' cognitive ability, and time spent on physical exercise was more strongly related to girls' cognitive ability in a positive manner. Besides, time spent attending interest classes on weekdays had a stronger negative relationship with cognitive ability among students from wealthy families, and time spent watching TV and physical exercise had stronger negative and positive effects on the cognitive ability among students from economically disadvantaged families, respectively. The theoretical and practical implications of the findings regarding the role of extra-curricular activities on adolescent development are discussed.

Psychological suzhi moderates state anxiety and heart rate responses to acute stress in male adolescents.

Psychological suzhi has increasingly been recognized to buffer chronic stress and improve mental health. However, few studies have examined the role of psychological suzhi in the response to acute stress. The current study aimed to investigate whether psychological suzhi can buffer state anxiety and heart rate responses to acute stress in adolescents. Healthy male adolescents (N = 63) were classified into high (N = 30) or low (N = 33) psychological suzhi groups based on the Chinese norm of adolescent psychological suzhi scale-simplified version. They completed both the modified Trier Social Stress Test for Groups and a specific control condition, with heart rate and state anxiety assessed. Data were analysed with mixed-design repeated measures analysis of variance. The results showed that the modified Trier Social Stress Test for Groups condition effectively induced an increase in state anxiety and heart rate. Furthermore, individuals with higher levels of psychological suzhi showed lower state anxiety and heart rate stress responses. The present study indicated that psychological suzhi is an important internal resource against acute stress, which may inform interventions for male adolescents exposed to acute stress.

Parent-Child Discrepancies in Perceived Parent-Child Communication and Depressive Symptoms in Early Adolescents in China.

Although recent studies demonstrated that parent-child discrepancies in the perceived family processes were associated with children's developmental outcomes, few studies have addressed this issue in different types of families in mainland China. The present study investigated that how discrepancies in parents' and adolescents' perceptions of parent-adolescent communication were associated with early adolescent depressive symptoms in a nationally representative sample (N = 15,377) with 7010 father-adolescent dyads (adolescents: Mage = 14.24 years, SD = 1.25 years; 5960 adolescents from two-parent families, 443 adolescents from single-father families) and 8367 mother-adolescent dyads (adolescents: Mage = 14.02 years, SD = 1.18 years; 6670 adolescents from two-parent families, 1362 adolescents from single-mother families) in China. Adolescent respondents completed a measure of depressive symptoms and all informants reported on the perceived levels of parent-adolescent communication. Results indicated that adolescents reported parent-child communication more negatively than did their parents. Father-adolescent discrepancies were also greater in intact families than non-intact families. Polynomial regression analyses indicated that while there was a significant interactive effect of father-reported and adolescent-reported father-adolescent communication in Chinese two-parent families, no significant interaction was found for mother-adolescent dyad. Besides, adolescent-reported mother-child communication interacted with mother-reported communication in Chinese single-mother families only. The findings clarify parent-adolescent discrepancies in parent-child communication in different types of families in China and they have theoretical and practical implications on the role of discrepancies in parents and adolescent children on perceived parent-adolescent communication in early adolescent depressive symptoms.

Cyberbullying victimization and depression among adolescents: A meta-analysis.

Cyberbullying is a category of bullying that occurs in the digital realm/medium of electronic text. In this study, we used meta-analysis to explore the relation between cyberbullying victimization and depression. We investigated 57 empirical studies from 17 countries (74 effect sizes and 105, 440 participants). The results showed that there was a significant positive relation between cyberbullying victimization and depression (r = .291, 95% CI = [.246, .335]). Moreover, we found that gender, age and publication year significantly moderated the relation between cyberbullying victimization and depression.

Parent-Adolescent Communication and Early Adolescent Depressive Symptoms: The Roles of Gender and Adolescents' Age.

Positive parent-adolescent communication has been found to be negatively related to adolescent depressive symptoms; however, few studies have investigated the moderating effects of adolescent gender and age on this relationship, especially during early adolescence in China. The present study investigated the joint moderating effects of adolescent gender and age on the linkage of father-adolescent and mother-adolescent communication with adolescents' depressive symptoms. A total of 11,455 Chinese junior high school students (M age = 14.15 years, SD = 1.22 years; 49.86% boys; N grade7 = 5712, N grade9 = 5743) completed ad hoc questionnaires of parent-adolescent communication and depressive symptoms. Multiple linear regression analyses were conducted. Results indicated that gender and age jointly moderated the association between parental communication and adolescent depressive symptoms. Specifically, for girls, the negative effects of both father-adolescent and mother-adolescent communication on depressive symptoms were stronger in 9th grade students than in 7th grade students, while for boys, the negative effects were not different between 7th grade students and 9th grade students. These findings suggest that in China, the protective effects of positive parent-adolescent communication on adolescents' depressive symptoms may be most salient among senior-grade girls in junior high school.

Mediating Effect of Dark Triad Personality Traits on the Relationship Between Parental Emotional Warmth and Aggression.

Previous research has indicated that positive parenting practices, such as parental emotional warmth, are associated with less negative outcomes in children. These negative outcomes can present during adolescence and during emerging adulthood. One negative consequence can be aggression, a problematic outcome with its own myriad consequences. The current study aimed to examine the effect of parental emotional warmth on adolescent and young adult aggression and the mediating effect of Dark Triad personality traits (Machiavellianism, psychopathy, and narcissism) on this relationship. The sample included 530 Chinese student participants who completed an online questionnaire survey: 241 adolescents aged 15 to 18 years (M = 17.24, SD = 1.00) and 289 young adults aged 19 to 23 years (M = 20.12, SD = 1.03). The results revealed that the association between parental emotional warmth and aggression was fully mediated by Machiavellianism and psychopathy, and the mediating effects of Machiavellianism and psychopathy had no age differences between adolescents and young adults. These results may clinically suggest that paying attention to parental emotional warmth, Machiavellianism, and psychopathy in adolescents and young adults could yield a better understanding of their current and continuous functioning, especially for aggression.

Mediating effect of psychological suzhi on the relationship between perceived social support and self-esteem.

This study examined the mediating role of psychological suzhi on the relationship between perceived social support and self-esteem in a sample of 612 Chinese secondary school students (grades 7-12, mean age 15.20 ± 1.73 years). Structural equation modeling revealed that the association between perceived social support and self-esteem was partially mediated by psychological suzhi, and perceived social support not only directly affected students' self-esteem but also indirectly affected students' self-esteem through psychological suzhi. Moreover, the mediating effect of psychological suzhi showed transgender and cross school section stability. These findings can help us understand how perceived social support affects self-esteem, from the perspective of psychological suzhi.

Relationship between study weariness, self compassion and problem behaviors of left behind adolescents / 中国学校卫生

Objective@#To analyze the association of study weariness with self compassion and problem behaviors among left behind adolescents, and to explore the moderating role of self compassion in this association.@*Methods@#By using convenient sampling method, 1 285 middle school students from two ordinary middle schools in a county of Dazhou City, Sichuan Province were selected, including 895 left behind adolescents. Study weariness, self compassion and problem behavior were assessed through questionnaire survey.@*Results@#The scores of self compassion and externalizing behaviors in boys were higher than that of girls, while the scores of internalizing behaviors of girls were higher than that of boys ( t =2.13, 1.98, -2.05, P <0.05); the scores of study weariness and internalizing behaviors among left behind adolescents were higher than those of non left behind adolescents, while the scores of self compassion of nonleft behind adolescents were higher than those of left behind adolescents ( t =2.01, 2.08, -1.99, P <0.05). The scores of externalizing behaviors among only one child and junior high school students were higher than that of non only one children and high school students, while the scores of internalization problem behaviors of high school students were higher than that of junior high school students ( t =1.95, 2.03, -2.02, P <0.05). There was a positive correlation between study weariness and problem behavior ( t =0.37， P <0.01), a negative correlation between self compassion and problem behavior ( t =-0.49， P < 0.01 ), and between study weariness and self compassin ( t =-0.46， P <0.01).@*Conclusion@#There is a positive correlation between study weariness and behaviral problems among left behind teenagers. The cultivation of self compassion ability of left behind teenagers should be encouraged and promoted.

Study protocol for a network meta-analysis of digital-technology-based psychotherapies for PTSD in adults.

INTRODUCTION: Studies on various types of digital-technology-based psychotherapies (DTPs) have indicated that they are effective for post-traumatic stress disorder (PTSD) symptom relief among adults. The intervention efficacy or effectiveness hierarchy, however, is still not clear. Therefore, we propose to conduct a network meta-analysis to assess the relative effectiveness of various types of DTPs. We aim to establish the differential effectiveness of these therapies in terms of symptom reduction and provide high-quality evidence for treating PTSD. METHODS AND ANALYSES: We will search Embase, CINAHL, MEDLINE, HealthSTAR, the Cochrane Library, PsycINFO, PubMed, the Chinese Biomedical Literature Database, clinical trials (eg, ClinicalTrials.gov) and other academic platforms for relevant studies, mainly in English and Chinese (as we plan to conduct a trial on PTSD patients in Wuhan, China, based on the results of this network meta-analysis), from inception to October 2020. Randomised controlled trials (RCTs) and meta-analyses investigating the effectiveness of any DTPs for PTSD patients for any controlled condition will be included. The number of intervention sessions and the research duration are unlimited; the effects for different durations will be tested via sensitivity analysis. For this project, the primary measure of outcome will be PTSD symptoms at the end of treatment using raw scores for one widely used PTSD scale, PCL-5. Secondary outcome measures will include (1) dropout rate; (2) effectiveness at longest follow-up, but not more than 12 months and (3) patients' functional recovery ratio (such as the return-to-work ratio or percentage of sick leave). Bayesian network meta-analysis will be conducted for all relative outcome measures. We will perform subgroup analysis and sensitivity analysis to see whether the results are influenced by study characteristics. The Grading of Recommendations, Assessments, Development, and Evaluation framework will be adopted to evaluate the quality of evidence contributing to network estimates of the primary outcome. ETHICS AND DISSEMINATION: The researchers of the primary trials already have had ethical approval for the data used in our study. We will present the results of this meta-analysis at academic conferences and publish them in peer-reviewed journals. PROSPERO REGISTRATION NUMBER: CRD42020173253.

Longitudinal association between school climate and depressive symptoms: The mediating role of psychological suzhi.

The protective role of students' perceptions of school climate against mental health problems has been supported in previous research, yet relatively little is known about the mechanism underlying school climate's influence on Chinese youths' depressive symptoms. Guided by the process-person-context-time model, this study examined the mediating effect of psychological suzhi (a Chinese cultural construct comprising a hierarchical, integrated set of positive psychological qualities) on the longitudinal association between perceived school climate and depressive symptoms among Chinese adolescents. Students (N = 1,151; 52.2% boys; mean [M]age = 16.24, standard deviation [SD] = 0.70) from one Chinese high school participated in a 3-wave (each wave was 6 months apart) longitudinal study. Multilevel mediation models were used to analyze between- and within-person effects on the longitudinal association between perceived school climate and depressive symptoms. The results indicated that students who perceived a more positive school climate reported lower depressive symptoms than students who perceived a more negative school climate (between-person effect); however, students who perceived their school climate positively did not always directly report lower depressive symptoms across time (within-person effect). Psychological suzhi mediated the association between perceived school climate and depressive symptoms at both the between- and within-person levels. Students who perceived a more positive school climate had increased psychological suzhi, which, in turn, decreased their depressive symptoms. The findings provided implications for school-based mental health prevention services by highlighting the need for promoting both positive school climate and students' psychological suzhi. (PsycInfo Database Record (c) 2020 APA, all rights reserved).