[ELISA-based determination of natural antibodies to endothelial NO-synthase in human blood and tear]. / Opredelenieestestvennykh antitel k éndotelial'noi NO-sintaze b krovi i sleze cheloveka metodom immunofermentnogo analiza.

Research of natural antibodies (NAA) is a new trend in immunohistochemistry. NAA shape up, in biological fluids, as normal human endogenous low- and high-weight substances, e.g. angiotensin 2, bradykinin and others, and play the regulatory role in homeostasis. Endothelial NO-synthase (e-NOS) is a key enzyme in the path of NO shaping. An e-NOS alteration is important in the pathogenesis of diabetic microangiopathy including retinopathy. We determined, within our case study, the level of NAA to e-NOS in blood and tears of patients with diabetic retinopathy and in donors. The level of NAA to e-NOS was found to be twice higher in tears versus blood in all groups of patients. It depended on specific features and complications of diabetes mellitus.

[Diagnostic implications of detecting antibodies to angiotensin-converting enzyme and its substrates]. / Diagnosticheskaia vozmozhnost' opredeleniia antitel k angiotenzinprevrashchaiushchemu fermentu i ego substratam.

The results of the analysis of diagnostic significance of examination of natural antibodies (Nab) to agiotensin-converting enzyme (ACE) and its substrates in the serum of hypertensive patients indicate that concentration of Nab to ACE differ from this mean concentration in donors. An elevated level of Nab to ACE may be considered as a compensatory reaction to increased content of the enzyme in vascular endothelium and blood flow. The same patients were examined for antibodies to peptide angiotensin II (AT-II). Enzyme immunoassay has shown that a significantly elevated level of antibodies to AT-II was only in 5 examinees. The same patients had also high Nab to ACE. The study of a group of ischemic heart disease patients with adverse effects attributed to bronchial affection treated with enalapril and diroton (ACE inhibitors) demonstrates that deterioration of cough and external respiration function is not related to exacerbation of existing chronic pulmonary inflammation. None of the patients had elevated body temperature or inflammatory changes in the blood, other signs of inflammation. Enzyme immunoassay also proved that the initial level of Nab equaled mean value for donors or was insignificantly lower. Blood serum patients with side effects contained a significantly (p < 0.02) elevated quantities of Nab to bradikinin vs initial values. Thus, the proposed method of solid phase enzyme immunoassay quantifies Nab to ACE and its substrates in the patients.

Antibodies to delta sleep-inducing peptide in ultralow doses: study of the effect by enzyme immunoassay.

The effects of potentiated homeopathic preparations containing antibodies to delta sleep-inducing peptide in ultralow doses were studied by enzyme immunoassay. Experiments were performed with the following immunochemical reagents: antigens of delta sleep-inducing peptide conjugated to various macromolecular carriers and specific antigens. Antibodies to delta sleep-inducing peptide were synthesized in dilutions of C3, C6, C12, C50, and C200. Enzyme immunoassay showed that test preparations of antibodies in dilutions of 1:400-1:3200 produce the combined effect on immune complex formation. The proposed method holds much promise for identification of medicinal preparations in ultralow doses.

[Immunoenzyme analysis of natural antibodies to endogenous bioregulators in patients with systemic lupus erythematosus]. / Immunofermentnyi analiz estestvennykh antitel k éndogennym bioreguliatoram u bol'nykh sistemnoi krasnoi volchankoi.

Serum levels of autoantibodies to endogenous bioregulators (prostaglandin F2 alpha, angiotensin II, epinephrine, bovine serum albumin, dinitrophenol) were measured in patients with systemic and integumental lupus erythematosus and donors and the diagnostic significance of deviations of these levels from the norm was evaluated. A total of 75 patients with lupus erythematosus aged 19-54 years with disease lasting for 0.5 to 18 months were examined. Significant differences between patients and donors were observed as regards virtually all parameters except IgG to angiotensin II.

[Immunoglobulins, binding opioid peptides, biogenic amines, and opiates, in substance abusers]. / Immunoglobuliny, sviazyvaiushchie opiodnye peptidy, biogennye aminy i opiaty, u bol'nykh narkomanie.

Immunoglobulins binding morphine, biogenic amines, and an opioid peptide dermorphine were measured by solid-phase enzyme immunoassay in patients abusing narcotics. The patients' ages varied from 20 to 40, with the duration of narcotic use 1-17 years. Narcotic dependence was found to involve increased production of immunoglobulins binding opioid and monoamine neuromediators. Enzyme immunoassay of antibodies to opiates detects latent forms of narcomania in cases when the narcotic is not present in the body. These results serve the basis for creating a new method for analysis of narcotic abuse and evaluation of the duration of their use, which can be used in practical medicine and in forensic medical expert evaluations.

[Immunoenzyme technique for detection of natural antibodies to bradykinin in cardiovascular diseases]. / Immunofermentnyi metod vyiavleniia estestvennykh antitel protiv bradikinina pri serdechno-sosudistykh zabolevaniiakh.

A method for detecting natural antibodies to bradykinin by ELISA has been developed. A polymeric matrix model of peptide antigen has been synthesized for this purpose. Optimal conditions for detecting antigen and antibodies in the sera of donors and cardiovascular patients have been chosen. The level of antibodies to bradykinin is constant in donors and shifted in patients.

[Detection of natural anti-angiotensin converting enzyme antibodies in human serum using immunoenzyme technique ]. / Opredelenie estestvennykh antitel k angiotenzinprevrashchaiushchemu frmentu v syvorotke krovi cheloveka immunofermentnym metodom.

Solid-phase enzyme immunoassay (EIA) was developed for detecting natural antibodies to angiotensine-converting enzyme (ACE). Optimal conditions for detecting natural anti-ACE by EIA in the sera of donors and patients with disorders of arterial pressure are selected. The findings indicate that the level of natural anti-ACE is normally constant, while in the patients it is increased in 50% cases.

[Immunoenzyme assay for detection of natural antibodies against vasopressin in systemic lupus erythematosus]. / Vyiavlenie estestvennykh antitel protiv vazopressina pri sistemnoi krasnoi volchanke immunofermentnym metodom.

A solid-phase enzyme immunoassay procedure for detecting natural antibodies to vasopressin (VP) is developed. For this purpose, a VP antigen is synthesized on polymer matrix. Optimal conditions of enzyme immunoassay for detecting antibodies to this antigen in the sera of donors and patients with systemic lupus erythematosus (SLE) are selected. The level of anti-VP antibodies is constant in donors and shifted in SLE patients. Changes in the level of natural antibodies to VP correlate with immunochemical parameters.

[Comparative study on the effect of antiatherosclerotic diet enriched with polyunsaturated omega-3 fatty acids of plant and animal origin on the level of natural antibodies against catecholamines in patients with cardiovascular diseases]. / Sravnitel'nyi analiz vliianiia protivoateroskleroticheskikh diet, obogashchennykh PNZhK omega-3 pastitel'nogo i zhivotnogo proiskhozhdeniia na uroven' estestvennykh antitel k katekholaminam u bol'hykh serdechno-sosudistymi zabolevaniiami.

The levels of natural antibodies against catecholamines in 138 patients with cardiovascular diseases was studied and the comparative analysis of influence of antiatherosclerotic diets with different origin of PUFA omega-3 on dynamic of these parameters was made. For the first time discovered universal action of diets with PUFA omega-3 vegetable and animal origin on parameters of humoral immunity: in case of primary excess of norm of the contents of natural antibodies to adrenaline, noradrenaline and dopamine as a result of treatment these parameters were reduced or did not change; and at is primary a low their level--parameters increased in most cases. The greatest immunocorrection effect was rendered by diet, enriched PUFA omega-3 of freshwater fishes fat.

[Determination of natural antibodies to catecholamines in health and disease]. / Opredelenie estestvennykh antitel k katekholaminam v norme i pri patologii.

AIM: Enzyme immunoassay (EIA) determination of the level of natural antibodies (n-Ab) to catecholamines in patients with metabolic disturbances and cardiovascular diseases, correlation between content of n-Ab to these bioregulators and clinical diagnosis, biochemical indices. MATERIALS AND METHODS: EIA with specially synthetized artificial antigens was used to determine n-Ab to catecholamines (adrenaline, noradrenaline, dopamin) in 41 patients with cardiovascular diseases and metabolic disturbances (ischemic heart disease, essential hypertension, hyperlipoproteinemia) and in 10 healthy subjects (donors, controls). RESULTS: Changes in the levels of antibodies to catecholamines correspond to the clinical diagnosis. Interrelations exist between n-Ab to catecholamines and biochemical parameters in the disease. CONCLUSION: The trends in the amount of n-Ab to catecholamines are a unique marker of metabolic disturbances of these endogenic substances and can be used in diagnosis.